Crystal structure of GeoCas9 HNH domain bound to S78A mutant anti-CRISPR AcrIIC1

entry_id	10vb
deposition_date	2026-02-10
last_revision	2026-04-29
title	Crystal structure of GeoCas9 HNH domain bound to S78A mutant anti-CRISPR AcrIIC1
keywords	Nuclease, Cas9, Inhibitor, Anti-CRISPR, CRISPR, mutant, DNA BINDING PROTEIN; DNA BINDING PROTEIN
method	X-RAY DIFFRACTION

rg_guinier	39.95 Å
rg_electron	39.71 Å
i0	275790000.00
molecular_weight	134050.0 kDa
excluded_volume	167300 ų
envelope_volume	233940 ų
shell_volume	49887 ų
envelope_diameter	141.3 Å
shell_rg	45.01 Å
envelope_rg	38.41 Å
shape_rg	39.71 Å
total_rg	40.02 Å
total_atoms	9444
n_residues	1159
n_harmonics	20
q_range	— – 0.5000 Å−1
n_points	101
shell_type	directional
solvent_density	0.3340 e/ų
contrast_shell	0.0300 e/ų
crysol_version	4.1.3

dmax	132.0 Å
rg_real	39.89 Å
rg_real_error	1.17 Å
i0_real	2.7580e+08
i0_real_error	4.1040e+06
rg_reciprocal	39.93 Å
i0_reciprocal	275800000.0000
total_estimate	0.8725
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	52.8 Å
skewness	0.250
kurtosis	-0.275
angular_range	— – 0.2000 Å−1
current_alpha	0.0000
highest_alpha	13960000.0000
n_real_points	41
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.825; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.998; Smooth: 0.865

Unit Cell

length_a	83.478 Å
length_b	90.469 Å
length_c	87.218 Å
angle_alpha	90.00 °
angle_beta	93.82 °
angle_gamma	90.00 °
z_pdb	12

Symmetry

space_group	P 1 21 1
int_tables_number	4
crystal_system	monoclinic

Refinement

resolution	2.300 Å
resolution_low	45.230 Å
r_factor	0.2130
r_work	0.2094
r_free	0.2792

Diffraction

temperature

100.0 K

Files (7)

• pr_distribution /app/data/pr_computation/0v/10vb/pr_distribution.json
• pr_output /app/data/pr_computation/0v/10vb/10vb.pr.out
• pr_plot /app/data/visualization/pr_computation/0v/10vb/10vb_pr_distribution.png
• saxs_curve_dat /app/data/saxs_computation/0v/10vb/10vb.dat
• saxs_fit /app/data/saxs_computation/0v/10vb/10vb.fit
• saxs_log /app/data/saxs_computation/0v/10vb/10vb.log
• saxs_plot /app/data/visualization/saxs_computation/0v/10vb/10vb_saxs_profile.png

Curves (3)

fit	/app/data/saxs_computation/0v/10vb/10vb.fit
pddf	/app/data/pr_computation/0v/10vb/10vb.pr.out
profile	/app/data/saxs_computation/0v/10vb/10vb.dat