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10vc

Crystal structure of GeoCas9 HNH domain bound to anti-CRISPR AcrIIC1

Method: X-RAY DIFFRACTION D_max: 130.7 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	10vc
deposition_date	2026-02-10
last_revision	2026-04-29
title	Crystal structure of GeoCas9 HNH domain bound to anti-CRISPR AcrIIC1
keywords	Nuclease, Cas9, Inhibitor, Anti-CRISPR, CRISPR, DNA BINDING PROTEIN; DNA BINDING PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	39.55 Å
rg_electron	39.32 Å
i0	277250000.00
molecular_weight	134140.0 kDa
excluded_volume	167350 Å³
envelope_volume	230700 Å³
shell_volume	49562 Å³
envelope_diameter	141.6 Å
shell_rg	44.66 Å
envelope_rg	38.13 Å
shape_rg	39.32 Å
total_rg	39.64 Å
total_atoms	9450
n_residues	1159
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	130.7 Å
rg_real	39.48 Å
rg_real_error	1.31 Å
i0_real	2.7720e+08
i0_real_error	5.1440e+06
rg_reciprocal	39.53 Å
i0_reciprocal	277300000.0000
total_estimate	0.8719
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	52.3 Å
skewness	0.243
kurtosis	-0.282
angular_range	— – 0.2000 Å⁻¹
current_alpha	0.0000
highest_alpha	12450000.0000
n_real_points	41
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.820; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.870

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	CRISPR-associated endonuclease Cas9
formula_weight	13078.8 kDa
ec_number	3.1.-.-
src_method	man
entity_count	6

Polymer

type	polypeptide(L)
strand_id	A,C,E,G,I,K
atom_count	10744

SGGTGHDIVKFKLWSEQNGRCAYSLQPIEIERLLEPGYVEVDHVIPYSRSLDDSYTNKVLVLTRENREKGNRIPAEYLGVGTERWQQFETFVLTNKQFSKKKRDRLLRLHY

Source

organism	Geobacillus stearothermophilus
ncbi_taxonomy_id	1422
host_organism	Escherichia coli BL21(DE3)

Entity 2 polymer

description	Anti-CRISPR protein (AcrIIC1)
formula_weight	9637.9 kDa
src_method	man
entity_count	6

Polymer

type	polypeptide(L)
strand_id	B,D,F,H,J,L

MANKTYKIGKNAGYDGCGLCLAAISENEAIKVKYLRDICPDYDGDDKAEDWLRWGTDSRVKAAALEMEQYAYTSVGMASCWEFVEL

Source

organism	Neisseria meningitidis
ncbi_taxonomy_id	487
host_organism	Escherichia coli BL21(DE3)

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	1294

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6. Citations (1)

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length_a	83.529 Å
length_b	89.252 Å
length_c	86.940 Å
angle_alpha	90.00 °
angle_beta	93.65 °
angle_gamma	90.00 °
z_pdb	12

space_group	P 1 21 1
int_tables_number	4
crystal_system	monoclinic

resolution	1.790 Å
resolution_low	29.590 Å
r_factor	0.1963
r_work	0.1944
r_free	0.2330

fit	/app/data/saxs_computation/0v/10vc/10vc.fit
pddf	/app/data/pr_computation/0v/10vc/10vc.pr.out
profile	/app/data/saxs_computation/0v/10vc/10vc.dat

Crystal structure of GeoCas9 HNH domain bound to anti-CRISPR AcrIIC1

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)