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1a9n

;CRYSTAL STRUCTURE OF THE SPLICEOSOMAL U2B''-U2A' PROTEIN COMPLEX BOUND TO A FRAGMENT OF U2 SMALL NUCLEAR RNA ;

Method: X-RAY DIFFRACTION D_max: 110.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1a9n
deposition_date	1998-04-08
title	;CRYSTAL STRUCTURE OF THE SPLICEOSOMAL U2B''-U2A' PROTEIN COMPLEX BOUND TO A FRAGMENT OF U2 SMALL NUCLEAR RNA ;
keywords	COMPLEX (NUCLEAR PROTEIN-RNA), RNA, SNRNP, RIBONUCLEOPROTEIN, RNA BINDING PROTEIN-RNA COMPLEX; RNA BINDING PROTEIN/RNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	33.54 Å
rg_electron	31.12 Å
i0	110527000.00
molecular_weight	74666.0 kDa
excluded_volume	89723 Å³
envelope_volume	116400 Å³
shell_volume	32538 Å³
envelope_diameter	112.4 Å
shell_rg	36.42 Å
envelope_rg	31.05 Å
shape_rg	30.96 Å
total_rg	31.99 Å
total_atoms	5188
n_residues	571
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	110.2 Å
rg_real	33.75 Å
rg_real_error	0.90 Å
i0_real	1.1050e+08
i0_real_error	1.7160e+06
rg_reciprocal	33.62 Å
i0_reciprocal	110500000.0000
total_estimate	0.8422
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	32.8 Å
skewness	0.477
kurtosis	-0.439
angular_range	— – 0.2350 Å⁻¹
current_alpha	0.0000
highest_alpha	7379000.0000
n_real_points	48
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.846; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.809; Smooth: 0.598

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	;RNA (5'-R(CPCPUPGPGPUPAPUPUPGPCPAPGPUPAPCPCPUPCPCPAPGP* GP*U)-3') ;
formula_weight	7622.5 kDa
src_method	man
entity_count	2

Polymer

type	polyribonucleotide
strand_id	Q,R
atom_count	5188

CCUGGUAUUGCAGUACCUCCAGGU

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606

Entity 2 polymer

description	U2A'
formula_weight	20226.6 kDa
mutation	C89D, S119C
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,C

MVKLTAELIEQAAQYTNAVRDRELDLRGYKIPVIENLGATLDQFDAIDFSDNEIRKLDGFPLLRRLKTLLVNNNRICRIGEGLDQALPDLTELILTNNSLVELGDLDPLASLKSLTYLCILRNPVTNKKHYRLYVIYKVPQVRVLDFQKVKLKERQEAEKMFKGKRGAQLAKDIAR

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli BL21(DE3)

Entity 3 polymer

description	;SPLICEOSOMAL U2B'' ;
formula_weight	11149.1 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	B,D

MDIRPNHTIYINNMNDKIKKEELKRSLYALFSQFGHVVDIVALKTMKMRGQAFVIFKELGSSTNALRQLQGFPFYGKPMRIQYAKTDSDIISKMRG

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli BL21(DE3)

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6. Citations (1)

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length_a	98.370 Å
length_b	128.240 Å
length_c	66.650 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	P 21 21 2
int_tables_number	18
crystal_system	—

resolution	2.380 Å
resolution_low	15.000 Å
r_factor	—
r_work	0.2820
r_free	0.3280

fit	/app/data/saxs_computation/a9/1a9n/1a9n.fit
pddf	/app/data/pr_computation/a9/1a9n/1a9n.pr.out
profile	/app/data/saxs_computation/a9/1a9n/1a9n.dat

;CRYSTAL STRUCTURE OF THE SPLICEOSOMAL U2B''-U2A' PROTEIN COMPLEX BOUND TO A FRAGMENT OF U2 SMALL NUCLEAR RNA ;

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 polymer

Polymer

Source

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)