5mqf

Cryo-EM structure of a human spliceosome activated for step 2 of splicing (C* complex)

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Pre-mRNA-processing-splicing factor 8

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PRP8_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–2335； UniProt 1–2335

116 kDa U5 small nuclear ribonucleoprotein component

OrganismNot specified

UniProt Q15029

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	U5S1_HUMAN
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–972； UniProt 1–972

SNW domain-containing protein 1

OrganismNot specified

UniProt Q13573

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SNW1_HUMAN
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain C； PDBConstruct 1–536； UniProt 1–536

Pleiotropic regulator 1

OrganismNot specified

UniProt O43660

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PLRG1_HUMAN
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain D； PDBConstruct 1–515； UniProt 1–514

Pre-mRNA-processing factor 17

OrganismNot specified

UniProt O60508

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PRP17_HUMAN
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain E； PDBConstruct 1–579； UniProt 1–579

U5 small nuclear ribonucleoprotein 40 kDa protein

OrganismNot specified

UniProt Q96DI7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SNR40_HUMAN
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain F； PDBConstruct 1–357； UniProt 1–357

Pre-mRNA-processing factor 19

OrganismNot specified

UniProt Q9UMS4

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PRP19_HUMAN
Isoform	—
PDB entities	7
Chains and sequence ranges	Author chain G； PDBConstruct 1–504； UniProt 1–504 Author chain H； PDBConstruct 1–504； UniProt 1–504 Author chain I； PDBConstruct 1–504； UniProt 1–504 Author chain J； PDBConstruct 1–504； UniProt 1–504

Pre-mRNA-splicing factor SPF27

OrganismNot specified

UniProt O75934

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SPF27_HUMAN
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain K； PDBConstruct 1–225； UniProt 1–225

Cell division cycle 5-like protein

OrganismNot specified

UniProt Q99459

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CDC5L_HUMAN
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain L； PDBConstruct 1–802； UniProt 1–802

Pre-mRNA-splicing factor SYF1

OrganismNot specified

UniProt Q9HCS7

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SYF1_HUMAN
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain M； PDBConstruct 1–855； UniProt 1–855

Pre-mRNA-splicing factor SYF2

OrganismNot specified

UniProt O95926

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SYF2_HUMAN
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain N； PDBConstruct 1–243； UniProt 1–243

Crooked neck-like protein 1

OrganismNot specified

UniProt Q9BZJ0

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CRNL1_HUMAN
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain O； PDBConstruct 1–848； UniProt 1–848

Pre-mRNA-splicing factor RBM22

OrganismNot specified

UniProt Q9NW64

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RBM22_HUMAN
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain P； PDBConstruct 1–420； UniProt 1–420

Protein BUD31 homolog

OrganismNot specified

UniProt P41223

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	BUD31_HUMAN
Isoform	—
PDB entities	14
Chains and sequence ranges	Author chain Q； PDBConstruct 1–144； UniProt 1–144

Spliceosome-associated protein CWC15 homolog

OrganismNot specified

UniProt Q9P013

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CWC15_HUMAN
Isoform	—
PDB entities	15
Chains and sequence ranges	Author chain R； PDBConstruct 1–229； UniProt 1–229

Serine/arginine repetitive matrix protein 2

OrganismNot specified

UniProt Q9UQ35

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SRRM2_HUMAN
Isoform	—
PDB entities	16
Chains and sequence ranges	Author chain S； PDBConstruct 1–2752； UniProt 1–2752

Pre-mRNA-splicing factor CWC22 homolog

OrganismNot specified

UniProt Q9HCG8

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CWC22_HUMAN
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain T； PDBConstruct 1–908； UniProt 1–908

Intron-binding protein aquarius

OrganismNot specified

UniProt O60306

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	AQR_HUMAN
Isoform	—
PDB entities	18
Chains and sequence ranges	Author chain U； PDBConstruct 1–1485； UniProt 1–1485

Peptidyl-prolyl cis-trans isomerase-like 1

OrganismNot specified

UniProt Q9Y3C6

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PPIL1_HUMAN
Isoform	—
PDB entities	19
Chains and sequence ranges	Author chain V； PDBConstruct 1–166； UniProt 1–166

;U2 small nuclear ribonucleoprotein A' ;

OrganismNot specified

UniProt P09661

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RU2A_HUMAN
Isoform	—
PDB entities	20
Chains and sequence ranges	Author chain W； PDBConstruct 1–255； UniProt 1–255

;U2 small nuclear ribonucleoprotein B'' ;

OrganismNot specified

UniProt P08579

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RU2B_HUMAN
Isoform	—
PDB entities	21
Chains and sequence ranges	Author chain X； PDBConstruct 1–225； UniProt 1–225

Small nuclear ribonucleoprotein Sm D2

OrganismNot specified

UniProt P62316

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SMD2_HUMAN
Isoform	—
PDB entities	22
Chains and sequence ranges	Author chain a； PDBConstruct 1–118； UniProt 1–118 Author chain h； PDBConstruct 1–118； UniProt 1–118

Small nuclear ribonucleoprotein F

OrganismNot specified

UniProt P62306

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RUXF_HUMAN
Isoform	—
PDB entities	23
Chains and sequence ranges	Author chain b； PDBConstruct 1–86； UniProt 1–86 Author chain i； PDBConstruct 1–86； UniProt 1–86

Small nuclear ribonucleoprotein E

OrganismNot specified

UniProt P62304

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RUXE_HUMAN
Isoform	—
PDB entities	24
Chains and sequence ranges	Author chain c； PDBConstruct 1–92； UniProt 1–92 Author chain j； PDBConstruct 1–92； UniProt 1–92

Small nuclear ribonucleoprotein G

OrganismNot specified

UniProt P62308

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RUXG_HUMAN
Isoform	—
PDB entities	25
Chains and sequence ranges	Author chain d； PDBConstruct 1–76； UniProt 1–76 Author chain k； PDBConstruct 1–76； UniProt 1–76

Small nuclear ribonucleoprotein Sm D3

OrganismNot specified

UniProt P62318

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SMD3_HUMAN
Isoform	—
PDB entities	26
Chains and sequence ranges	Author chain e； PDBConstruct 1–126； UniProt 1–126 Author chain l； PDBConstruct 1–126； UniProt 1–126

;Small nuclear ribonucleoprotein-associated proteins B and B' ;

OrganismNot specified

UniProt P14678

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RSMB_HUMAN
Isoform	—
PDB entities	27
Chains and sequence ranges	Author chain f； PDBConstruct 1–240； UniProt 1–240 Author chain m； PDBConstruct 1–240； UniProt 1–240

Small nuclear ribonucleoprotein Sm D1

OrganismNot specified

UniProt P62314

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SMD1_HUMAN
Isoform	—
PDB entities	28
Chains and sequence ranges	Author chain g； PDBConstruct 1–119； UniProt 1–119 Author chain n； PDBConstruct 1–119； UniProt 1–119

Peptidyl-prolyl cis-trans isomerase E

OrganismNot specified

UniProt Q9UNP9

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Eukaryotic initiation factor 4A-III × 1 （P38919） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PPIE_HUMAN
Isoform	—
PDB entities	29
Chains and sequence ranges	Author chain o； PDBConstruct 1–301； UniProt 1–301

Eukaryotic initiation factor 4A-III

OrganismNot specified

UniProt P38919

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） ATP-dependent RNA helicase DHX8 × 1 （Q14562） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	IF4A3_HUMAN
Isoform	—
PDB entities	30
Chains and sequence ranges	Author chain p； PDBConstruct 1–411； UniProt 1–411

ATP-dependent RNA helicase DHX8

OrganismNot specified

UniProt Q14562

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 41 RNA 5	Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） 116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） SNW domain-containing protein 1 × 1 （Q13573） Pleiotropic regulator 1 × 1 （O43660） Pre-mRNA-processing factor 17 × 1 （O60508） U5 small nuclear ribonucleoprotein 40 kDa protein × 1 （Q96DI7） Pre-mRNA-processing factor 19 × 4 （Q9UMS4） Pre-mRNA-splicing factor SPF27 × 1 （O75934） Cell division cycle 5-like protein × 1 （Q99459） Pre-mRNA-splicing factor SYF1 × 1 （Q9HCS7） Pre-mRNA-splicing factor SYF2 × 1 （O95926） Crooked neck-like protein 1 × 1 （Q9BZJ0） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Serine/arginine repetitive matrix protein 2 × 1 （Q9UQ35） Pre-mRNA-splicing factor CWC22 homolog × 1 （Q9HCG8） Intron-binding protein aquarius × 1 （O60306） Peptidyl-prolyl cis-trans isomerase-like 1 × 1 （Q9Y3C6） ;U2 small nuclear ribonucleoprotein A' ; × 1 （P09661） ;U2 small nuclear ribonucleoprotein B'' ; × 1 （P08579） Small nuclear ribonucleoprotein Sm D2 × 2 （P62316） Small nuclear ribonucleoprotein F × 2 （P62306） Small nuclear ribonucleoprotein E × 2 （P62304） Small nuclear ribonucleoprotein G × 2 （P62308） Small nuclear ribonucleoprotein Sm D3 × 2 （P62318） ;Small nuclear ribonucleoprotein-associated proteins B and B' ; × 2 （P14678） Small nuclear ribonucleoprotein Sm D1 × 2 （P62314） Peptidyl-prolyl cis-trans isomerase E × 1 （Q9UNP9） Eukaryotic initiation factor 4A-III × 1 （P38919） MINX pre-mRNA (intron) × 2 Human gene for small nuclear RNA U2 (snRNA U2) × 1 Homo sapiens U5 A small nuclear RNA × 1 Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA × 1	Consistent with protein count

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	DHX8_HUMAN
Isoform	—
PDB entities	31
Chains and sequence ranges	Author chain q； PDBConstruct 1–1218； UniProt 1–1220

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	5mqf
Deposition date deposition_date	2016-12-20
Structure title title	Cryo-EM structure of a human spliceosome activated for step 2 of splicing (C* complex)
Keywords keywords	Spliceosome, pre-mRNA splicing, macromolecular complex, splicing; SPLICING
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

5mqf__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

5mqf__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

5mqf__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	95.49 Å
Rg (electron density)	98.80 Å
Total Rg	118.40 Å
Atom count	52549
Residues	9175
Excluded volume	1218200 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`5mqf__assembly_1__model_1`	41-meric （41）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (35)

Entity 1 Entity 1 polymer

Description description	Pre-mRNA-processing-splicing factor 8
Molecular weight formula_weight	273974.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	55236

MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Entity 2 Entity 2 polymer

Description description	116 kDa U5 small nuclear ribonucleoprotein component
Molecular weight formula_weight	109560.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLHEDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLADLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVGIKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLNTERLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTDENLILSPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKAPTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVCKKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTKMYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIEVNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPSELPKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVVTFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQTKYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLCDELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPADCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSVFHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLELAKQDVVLNYPM

Entity 3 Entity 3 polymer

Description description	SNW domain-containing protein 1
Molecular weight formula_weight	61610.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	C

MALTSFLPAPTQLSQDQLEAEEKARSQRSRQTSLVSSRREPPPYGYRKGWIPRLLEDFGDGGAFPEIHVAQYPLDMGRKKKMSNALAIQVDSEGKIKYDAIARQGQSKDKVIYSKYTDLVPKEVMNADDPDLQRPDEEAIKEITEKTRVALEKSVSQKVAAAMPVRAADKLAPAQYIRYTPSQQGVAFNSGAKQRVIRMVEMQKDPMEPPRFKINKKIPRGPPSPPAPVMHSPSRKMTVKEQQEWKIPPCISNWKNAKGYTIPLDKRLAADGRGLQTVHINENFAKLAEALYIADRKAREAVEMRAQVERKMAQKEKEKHEEKLREMAQKARERRAGIKTHVEKEDGEARERDEIRHDRRKERQHDRNLSRAAPDKRSKLQRNENRDISEVIALGVPNPRTSNEVQYDQRLFNQSKGMDSGFAGGEDEIYNVYDQAWRGGKDMAQSIYRPSKNLDKDMYGDDLEARIKTNRFVPDKEFSGSDRRQRGREGPVQFEEDPFGLDKFLEEAKQHGGSKRPSDSSRPKEHEHEGKKRRKE

Entity 4 Entity 4 polymer

Description description	Pleiotropic regulator 1
Molecular weight formula_weight	57379.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	D

MVEEVQKHSVHTLVFRSLKRTHDMFVADNGKPVPLDEESHKRKMAIKLRNEYGPVLHMPTSKENLKEKGPQNATDSYVHKQYPANQGQEVEYFVAGTHPYPPGPGVALTADTKIQRMPSESAAQSLAVALPLQTKADANRTAPSGSEYRHPGASDRPQPTAMNSIVMETGNTKNSALMAKKAPTMPKPQWHPPWKLYRVISGHLGWVRCIAVEPGNQWFVTGSADRTIKIWDLASGKLKLSLTGHISTVRGVIVSTRSPYLFSCGEDKQVKCWDLEYNKVIRHYHGHLSAVYGLDLHPTIDVLVTCSRDSTARIWDVRTKASVHTLSGHTNAVATVRCQAAEPQIITGSHDTTIRLWDLVAGKTRVTLTNHKKSVRAVVLHPRHYTFASGSPDNIKQWKFPDGSFIQNLSGHNAIINTLTVNSDGVLVSGADNGTMHLWDWRTGYNFQRVHAAAVQPGSLDESGIFACAFDQSESRLLTAEADKTIKVYREDDDTATEETHPVSWKPEIIKRKRF

Entity 5 Entity 5 polymer

Description description	Pre-mRNA-processing factor 17
Molecular weight formula_weight	65612.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	E

MSAAIAALAASYGSGSGSESDSDSESSRCPLPAADSLMHLTKSPSSKPSLAVAVDSAPEVAVKEDLETGVHLDPAVKEVQYNPTYETMFAPEFGPENPFRTQQMAAPRNMLSGYAEPAHINDFMFEQQRRTFATYGYALDPSLDNHQVSAKYIGSVEEAEKNQGLTVFETGQKKTEKRKKFKENDASNIDGFLGPWAKYVDEKDVAKPSEEEQKELDEITAKRQKKGKQEEEKPGEEKTILHVKEMYDYQGRSYLHIPQDVGVNLRSTMPPEKCYLPKKQIHVWSGHTKGVSAVRLFPLSGHLLLSCSMDCKIKLWEVYGERRCLRTFIGHSKAVRDICFNTAGTQFLSAAYDRYLKLWDTETGQCISRFTNRKVPYCVKFNPDEDKQNLFVAGMSDKKIVQWDIRSGEIVQEYDRHLGAVNTIVFVDENRRFVSTSDDKSLRVWEWDIPVDFKYIAEPSMHSMPAVTLSPNGKWLACQSMDNQILIFGAQNRFRLNKKKIFKGHMVAGYACQVDFSPDMSYVISGDGNGKLNIWDWKTTKLYSRFKAHDKVCIGAVWHPHETSKVITCGWDGLIKLWD

Entity 6 Entity 6 polymer

Description description	U5 small nuclear ribonucleoprotein 40 kDa protein
Molecular weight formula_weight	39359.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	F

MIEQQKRKGPELPLVPVKRQRHELLLGAGSGPGAGQQQATPGALLQAGPPRCSSLQAPIMLLSGHEGEVYCCKFHPNGSTLASAGFDRLILLWNVYGDCDNYATLKGHSGAVMELHYNTDGSMLFSASTDKTVAVWDSETGERVKRLKGHTSFVNSCYPARRGPQLVCTGSDDGTVKLWDIRKKAAIQTFQNTYQVLAVTFNDTSDQIISGGIDNDIKVWDLRQNKLTYTMRGHADSVTGLSLSSEGSYLLSNAMDNTVRVWDVRPFAPKERCVKIFQGNVHNFEKNLLRCSWSPDGSKIAAGSADRFVYVWDTTSRRILYKLPGHAGSINEVAFHPDEPIIISASSDKRLYMGEIQ

Entity 7 Entity 7 polymer

Description description	Pre-mRNA-processing factor 19
Molecular weight formula_weight	55245.5 kDa
EC 编号 ec_number	6.3.2.-
Source method src_method	nat
Copy count entity_count	4

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G,H,I,J

MSLICSISNEVPEHPCVSPVSNHVYERRLIEKYIAENGTDPINNQPLSEEQLIDIKVAHPIRPKPPSATSIPAILKALQDEWDAVMLHSFTLRQQLQTTRQELSHALYQHDAACRVIARLTKEVTAAREALATLKPQAGLIVPQAVPSSQPSVVGAGEPMDLGELVGMTPEIIQKLQDKATVLTTERKKRGKTVPEELVKPEELSKYRQVASHVGLHSASIPGILALDLCPSDTNKILTGGADKNVVVFDKSSEQILATLKGHTKKVTSVVFHPSQDLVFSASPDATIRIWSVPNASCVQVVRAHESAVTGLSLHATGDYLLSSSDDQYWAFSDIQTGRVLTKVTDETSGCSLTCAQFHPDGLIFGTGTMDSQIKIWDLKERTNVANFPGHSGPITSIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQLDNNFEVKSLIFDQSGTYLALGGTDVQIYICKQWTEILHFTEHSGLTTGVAFGHHAKFIASTGMDRSLKFYSL

Entity 8 Entity 8 polymer

Description description	Pre-mRNA-splicing factor SPF27
Molecular weight formula_weight	26163.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K

MAGTGLVAGEVVVDALPYFDQGYEAPGVREAAAALVEEETRRYRPTKNYLSYLTAPDYSAFETDIMRNEFERLAARQPIELLSMKRYELPAPSSGQKNDITAWQECVNNSMAQLEHQAVRIENLELMSQHGCNAWKVYNENLVHMIEHAQKELQKLRKHIQDLNWQRKNMQLTAGSKLREMESNWVSLVSKNYEIERTIVQLENEIYQIKQQHGEANKENIRQDF

Entity 9 Entity 9 polymer

Description description	Cell division cycle 5-like protein
Molecular weight formula_weight	92406.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L

MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKKTEWSREEEEKLLHLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKAAQRDNEEETTDDPRKLKPGEIDPNPETKPARPDPIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEARRLAALQKRRELRAAGIEIQKKRKRKRGVDYNAEIPFEKKPALGFYDTSEENYQALDADFRKLRQQDLDGELRSEKEGRDRKKDKQHLKRKKESDLPSAILQTSGVSEFTKKRSKLVLPAPQISDAELQEVVKVGQASEIARQTAEESGITNSASSTLLSEYNVTNNSVALRTPRTPASQDRILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQVVQTPNTVLSTPFRTPSNGAEGLTPRSGTTPKPVINSTPGRTPLRDKLNINPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQEREKELQHRYADLLLEKETLKSKF

Entity 10 Entity 10 polymer

Description description	Pre-mRNA-splicing factor SYF1
Molecular weight formula_weight	100148.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	M

MVVMARLSRPERPDLVFEEEDLPYEEEIMRNQFSVKCWLRYIEFKQGAPKPRLNQLYERALKLLPCSYKLWYRYLKARRAQVKHRCVTDPAYEDVNNCHERAFVFMHKMPRLWLDYCQFLMDQGRVTHTRRTFDRALRALPITQHSRIWPLYLRFLRSHPLPETAVRGYRRFLKLSPESAEEYIEYLKSSDRLDEAAQRLATVVNDERFVSKAGKSNYQLWHELCDLISQNPDKVQSLNVDAIIRGGLTRFTDQLGKLWCSLADYYIRSGHFEKARDVYEEAIRTVMTVRDFTQVFDSYAQFEESMIAAKMETASELGREEEDDVDLELRLARFEQLISRRPLLLNSVLLRQNPHHVHEWHKRVALHQGRPREIINTYTEAVQTVDPFKATGKPHTLWVAFAKFYEDNGQLDDARVILEKATKVNFKQVDDLASVWCQCGELELRHENYDEALRLLRKATALPARRAEYFDGSEPVQNRVYKSLKVWSMLADLEESLGTFQSTKAVYDRILDLRIATPQIVINYAMFLEEHKYFEESFKAYERGISLFKWPNVSDIWSTYLTKFIARYGGRKLERARDLFEQALDGCPPKYAKTLYLLYAQLEEEWGLARHAMAVYERATRAVEPAQQYDMFNIYIKRAAEIYGVTHTRGIYQKAIEVLSDEHAREMCLRFADMECKLGEIDRARAIYSFCSQICDPRTTGAFWQTWKDFEVRHGNEDTIKEMLRIRRSVQATYNTQVNFMASQMLKVSGSATGTVSDLAPGQSGMDDMKLLEQRAEQLAAEAERDQPLRAQSKILFVRSDASREELAELAQQVNPEEIQLGEDEDEDEMDLEPNEVRLEQQSVPAAVFGSLKED

Entity 11 Entity 11 polymer

Description description	Pre-mRNA-splicing factor SYF2
Molecular weight formula_weight	28780.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	N

MAAIAASEVLVDSAEEGSLAAAAELAAQKREQRLRKFRELHLMRNEARKLNHQEVVEEDKRLKLPANWEAKKARLEWELKEEEKKKECAARGEDYEKVKLLEISAEDAERWERKKKRKNPDLGFSDYAAAQLRQYHRLTKQIKPDMETYERLREKHGEEFFPTSNSLLHGTHVPSTEEIDRMVIDLEKQIEKRDKYSRRRPYNDDADIDYINERNAKFNKKAERFYGKYTAEIKQNLERGTAV

Entity 12 Entity 12 polymer

Description description	Crooked neck-like protein 1
Molecular weight formula_weight	100610.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	O

MTATVENLTFQKDTLGNAVDKNTSRLELRSYSLAGRHGSTEPLVLAWSSQFRRLTWGCALDALHRSPCVAASQHGVTHLIRSSRTPHSTRCRKEDAQPGHHGNGAASVTAQARGQRSVLQVPLPVPRSCLFSESFVVSVSSQSRFLASVPGTGVQRSTAADMAASTAAGKQRIPKVAKVKNKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELNDYKLRKRKTFEDNIRKNRTVISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQVNHARNIWDRAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFELRYKEVDRARTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFAKFEENQKEFERVRVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQYEEEVKANPHNYDAWFDYLRLVESDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINYALYEELEAKDPERTRQVYQASLELIPHKKFTFAKMWILYAQFEIRQKNLSLARRALGTSIGKCPKNKLFKVYIELELQLREFDRCRKLYEKFLEFGPENCTSWIKFAELETILGDIDRARAIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRLLQRTQHVKVWISFAQFELSSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDKERVDKLMPEKVKKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPDEDVDESES

Entity 13 Entity 13 polymer

Description description	Pre-mRNA-splicing factor RBM22
Molecular weight formula_weight	46959.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	P

MATSLGSNTYNRQNWEDADFPILCQTCLGENPYIRMTKEKYGKECKICARPFTVFRWCPGVRMRFKKTEVCQTCSKLKNVCQTCLLDLEYGLPIQVRDAGLSFKDDMPKSDVNKEYYTQNMEREISNSDGTRPVGMLGKATSTSDMLLKLARTTPYYKRNRPHICSFWVKGECKRGEECPYRHEKPTDPDDPLADQNIKDRYYGINDPVADKLLKRASTMPRLDPPEDKTITTLYVGGLGDTITETDLRNHFYQFGEIRTITVVQRQQCAFIQFATRQAAEVAAEKSFNKLIVNGRRLNVKWGRSQAARGKEKEKDGTTDSGIKLEPVPGLPGALPPPPAAEEEASANYFNLPPSGPPAVVNIALPPPPGIAPPPPPGFGPHMFHPMGPPPPFMRAPGPIHYPSQDPQRMGAHAGKHSSP

Entity 14 Entity 14 polymer

Description description	Protein BUD31 homolog
Molecular weight formula_weight	17032.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Q

MPKVKRSRKAPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYIFDLFYKRKAISRELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG

Entity 15 Entity 15 polymer

Description description	Spliceosome-associated protein CWC15 homolog
Molecular weight formula_weight	26674.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	R

MTTAARPTFEPARGGRGKGEGDLSQLSKQYSSRDLPSHTKIKYRQTTQDAPEEVRNRDFRRELEERERAAAREKNRDRPTREHTTSSSVSKKPRLDQIPAANLDADDPLTDEEDEDFEEESDDDDTAALLAELEKIKKERAEEQARKEQEQKAEEERIRMENILSGNPLLNLTGPSQPQANFKVKRRWDDDVVFKNCAKGVDDQKKDKRFVNDTLRSEFHKKFMEKYIK

Entity 16 Entity 16 polymer

Description description	Serine/arginine repetitive matrix protein 2
Molecular weight formula_weight	300255.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	S

MYNGIGLPTPRGSGTNGYVQRNLSLVRGRRGERPDYKGEEELRRLEAALVKRPNPDILDHERKRRVELRCLELEEMMEEQGYEEQQIQEKVATFRLMLLEKDVNPGGKEETPGQRPAVTETHQLAELNEKKNERLRAAFGISDSYVDGSSFDPQRRAREAKQPAPEPPKPYSLVRESSSSRSPTPKQKKKKKKKDRGRRSESSSPRRERKKSSKKKKHRSESESKKRKHRSPTPKSKRKSKDKKRKRSRSTTPAPKSRRAHRSTSADSASSSDTSRSRSRSAAAKTHTTALAGRSPSPASGRRGEGDAPFSEPGTTSTQRPSSPETATKQPSSPYEDKDKDKKEKSATRPSPSPERSSTGPEPPAPTPLLAERHGGSPQPLATTPLSQEPVNPPSEASPTRDRSPPKSPEKLPQSSSSESSPPSPQPTKVSRHASSSPESPKPAPAPGSHREISSSPTSKNRSHGRAKRDKSHSHTPSRRMGRSRSPATAKRGRSRSRTPTKRGHSRSRSPQWRRSRSAQRWGRSRSPQRRGRSRSPQRPGWSRSRNTQRRGRSRSARRGRSHSRSPATRGRSRSRTPARRGRSRSRTPARRRSRSRTPTRRRSRSRTPARRGRSRSRTPARRRSRTRSPVRRRSRSRSPARRSGRSRSRTPARRGRSRSRTPARRGRSRSRTPARRSGRSRSRTPARRGRSRSRTPRRGRSRSRSLVRRGRSHSRTPQRRGRSGSSSERKNKSRTSQRRSRSNSSPEMKKSRISSRRSRSLSSPRSKAKSRLSLRRSLSGSSPCPKQKSQTPPRRSRSGSSQPKAKSRTPPRRSRSSSSPPPKQKSKTPSRQSHSSSSPHPKVKSGTPPRQGSITSPQANEQSVTPQRRSCFESSPDPELKSRTPSRHSCSGSSPPRVKSSTPPRQSPSRSSSPQPKVKAIISPRQRSHSGSSSPSPSRVTSRTTPRRSRSVSPCSNVESRLLPRYSHSGSSSPDTKVKPETPPRQSHSGSISPYPKVKAQTPPGPSLSGSKSPCPQEKSKDSLVQSCPGSLSLCAGVKSSTPPGESYFGVSSLQLKGQSQTSPDHRSDTSSPEVRQSHSESPSLQSKSQTSPKGGRSRSSSPVTELASRSPIRQDRGEFSASPMLKSGMSPEQSRFQSDSSSYPTVDSNSLLGQSRLETAESKEKMALPPQEDATASPPRQKDKFSPFPVQDRPESSLVFKDTLRTPPRERSGAGSSPETKEQNSALPTSSQDEELMEVVEKSEEPAGQILSHLSSELKEMSTSNFESSPEVEERPAVSLTLDQSQSQASLEAVEVPSMASSWGGPHFSPEHKELSNSPLRENSFGSPLEFRNSGPLGTEMNTGFSSEVKEDLNGPFLNQLETDPSLDMKEQSTRSSGHSSSELSPDAVEKAGMSSNQSISSPVLDAVPRTPSRERSSSASSPEMKDGLPRTPSRRSRSGSSPGLRDGSGTPSRHSLSGSSPGMKDIPRTPSRGRSECDSSPEPKALPQTPRPRSRSPSSPELNNKCLTPQRERSGSESSVDQKTVARTPLGQRSRSGSSQELDVKPSASPQERSESDSSPDSKAKTRTPLRQRSRSGSSPEVDSKSRLSPRRSRSGSSPEVKDKPRAAPRAQSGSDSSPEPKAPAPRALPRRSRSGSSSKGRGPSPEGSSSTESSPEHPPKSRTARRGSRSSPEPKTKSRTPPRRRSSRSSPELTRKARLSRRSRSASSSPETRSRTPPRHRRSPSVSSPEPAEKSRSSRRRRSASSPRTKTTSRRGRSPSPKPRGLQRSRSRSRREKTRTTRRRDRSGSSQSTSRRRQRSRSRSRVTRRRRGGSGYHSRSPARQESSRTSSRRRRGRSRTPPTSRKRSRSRTSPAPWKRSRSRASPATHRRSRSRTPLISRRRSRSRTSPVSRRRSRSRTSVTRRRSRSRASPVSRRRSRSRTPPVTRRRSRSRTPTTRRRSRSRTPPVTRRRSRSRTPPVTRRRSRSRTSPITRRRSRSRTSPVTRRRSRSRTSPVTRRRSRSRTSPVTRRRSRSRTPPAIRRRSRSRTPLLPRKRSRSRSPLAIRRRSRSRTPRTARGKRSLTRSPPAIRRRSASGSSSDRSRSATPPATRNHSGSRTPPVALNSSRMSCFSRPSMSPTPLDRCRSPGMLEPLGSSRTPMSVLQQAGGSMMDGPGPRIPDHQRTSVPENHAQSRIALALTAISLGTARPPPSMSAAGLAARMSQVPAPVPLMSLRTAPAANLASRIPAASAAAMNLASARTPAIPTAVNLADSRTPAAAAAMNLASPRTAVAPSAVNLADPRTPTAPAVNLAGARTPAALAALSLTGSGTPPTAANYPSSSRTPQAPASANLVGPRSAHATAPVNIAGSRTAAALAPASLTSARMAPALSGANLTSPRVPLSAYERVSGRTSPPLLDRARSRTPPSAPSQSRMTSERAPSPSSRMGQAPSQSLLPPAQDQPRSPVPSAFSDQSRCLIAQTTPVAGSQSLSSGAVATTTSSAGDHNGMLSVPAPGVPHSDVGEPPASTGAQQPSALAALQPAKERRSSSSSSSSSSSSSSSSSSSSSSSSSGSSSSDSEGSSLPVQPEVALKRVPSPTPAPKEAVREGRPPEPTPAKRKRRSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSPSPAKPGPQALPKPASPKKPPPGERRSRSPRKPIDSLRDSRSLSYSPVERRRPSPQPSPRDQQSSSSERGSRRGQRGDSRSPSHKRRRETPSPRPMRHRSSRSP

Entity 17 Entity 17 polymer

Description description	Pre-mRNA-splicing factor CWC22 homolog
Molecular weight formula_weight	105646.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	T

MKSSVAQIKPSSGHDRRENLNSYQRNSSPEDRYEEQERSPRDRDYFDYSRSDYEHSRRGRSYDSSMESRNRDREKRRERERDTDRKRSRKSPSPGRRNPETSVTQSSSAQDEPATKKKKDELDPLLTRTGGAYIPPAKLRMMQEQITDKNSLAYQRMSWEALKKSINGLINKVNISNISIIIQELLQENIVRGRGLLSRSVLQAQSASPIFTHVYAALVAIINSKFPQIGELILKRLILNFRKGYRRNDKQLCLTASKFVAHLINQNVAHEVLCLEMLTLLLERPTDDSVEVAIGFLKECGLKLTQVSPRGINAIFERLRNILHESEIDKRVQYMIEVMFAVRKDGFKDHPIILEGLDLVEEDDQFTHMLPLEDDYNPEDVLNVFKMDPNFMENEEKYKAIKKEILDEGDTDSNTDQDAGSSEEDEEEEEEEGEEDEEGQKVTIHDKTEINLVSFRRTIYLAIQSSLDFEECAHKLLKMEFPESQTKELCNMILDCCAQQRTYEKFFGLLAGRFCMLKKEYMESFEGIFKEQYDTIHRLETNKLRNVAKMFAHLLYTDSLPWSVLECIKLSEETTTSSSRIFVKIFFQELCEYMGLPKLNARLKDETLQPFFEGLLPRDNPRNTRFAINFFTSIGLGGLTDELREHLKNTPKVIVAQKPDVEQNKSSPSSSSSASSSSESDSSDSDSDSSDSSSESSSEESDSSSISSHSSASANDVRKKGHGKTRSKEVDKLIRNQQTNDRKQKERRQEHGHQETRTERERRSEKHRDQNSSGSNWRDPITKYTSDKDVPSERNNYSRVANDRDQEMHIDLENKHGDPKKKRGERRNSFSENEKHTHRIKDSENFRRKDRSKSKEMNRKHSGSRSDEDRYQNGAERRWEKSSRYSEQSRESKKNQDRRREKSPAKQK

Entity 18 Entity 18 polymer

Description description	Intron-binding protein aquarius
Molecular weight formula_weight	173003.1 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	U

MAAPAQPKKIVAPTVSQINAEFVTQLACKYWAPHIKKKSPFDIKVIEDIYEKEIVKSRFAIRKIMLLEFSQYLENYLWMNYSPEVSSKAYLMSICCMVNEKFRENVPAWEIFKKKPDHFPFFFKHILKAALAETDGEFSLHEQTVLLLFLDHCFNSLEVDLIRSQVQQLISLPMWMGLQLARLELELKKTPKLRKFWNLIKKNDEKMDPEAREQAYQERRFLSQLIQKFISVLKSVPLSEPVTMDKVHYCERFIELMIDLEALLPTRRWFNTILDDSHLLVHCYLSNLVRREEDGHLFSQLLDMLKFYTGFEINDQTGNALTENEMTTIHYDRITSLQRAAFAHFPELYDFALSNVAEVDTRESLVKFFGPLSSNTLHQVASYLCLLPTLPKNEDTTFDKEFLLELLVSRHERRISQIQQLNQMPLYPTEKIIWDENIVPTEYYSGEGCLALPKLNLQFLTLHDYLLRNFNLFRLESTYEIRQDIEDSVSRMKPWQSEYGGVVFGGWARMAQPIVAFTVVEVAKPNIGENWPTRVRADVTINLNVRDHIKDEWEGLRKHDVCFLITVRPTKPYGTKFDRRRPFIEQVGLVYVRGCEIQGMLDDKGRVIEDGPEPRPNLRGESRTFRVFLDPNQYQQDMTNTIQNGAEDVYETFNIIMRRKPKENNFKAVLETIRNLMNTDCVVPDWLHDIILGYGDPSSAHYSKMPNQIATLDFNDTFLSIEHLKASFPGHNVKVTVEDPALQIPPFRITFPVRSGKGKKRKDADVEDEDTEEAKTLIVEPHVIPNRGPYPYNQPKRNTIQFTHTQIEAIRAGMQPGLTMVVGPPGTGKTDVAVQIISNIYHNFPEQRTLIVTHSNQALNQLFEKIMALDIDERHLLRLGHGEEELETEKDFSRYGRVNYVLARRIELLEEVKRLQKSLGVPGDASYTCETAGYFFLYQVMSRWEEYISKVKNKGSTLPDVTEVSTFFPFHEYFANAPQPIFKGRSYEEDMEIAEGCFRHIKKIFTQLEEFRASELLRSGLDRSKYLLVKEAKIIAMTCTHAALKRHDLVKLGFKYDNILMEEAAQILEIETFIPLLLQNPQDGFSRLKRWIMIGDHHQLPPVIKNMAFQKYSNMEQSLFTRFVRVGVPTVDLDAQGRARASLCNLYNWRYKNLGNLPHVQLLPEFSTANAGLLYDFQLINVEDFQGVGESEPNPYFYQNLGEAEYVVALFMYMCLLGYPADKISILTTYNGQKHLIRDIINRRCGNNPLIGRPNKVTTVDRFQGQQNDYILLSLVRTRAVGHLRDVRRLVVAMSRARLGLYIFARVSLFQNCFELTPAFSQLTARPLHLHIIPTEPFPTTRKNGERPSHEVQIIKNMPQMANFVYNMYMHLIQTTHHYHQTLLQLPPAMVEEGEEVQNQETELETEEEAMTVQADIIPSPTDTSCRQETPAFQTDTTPSETGATSTPEAIPALSETTPTVVGAVSAPAEANTPQDATSAPEETK

Entity 19 Entity 19 polymer

Description description	Peptidyl-prolyl cis-trans isomerase-like 1
Molecular weight formula_weight	18257.8 kDa
EC 编号 ec_number	5.2.1.8
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	V

MAAIPPDSWQPPNVYLETSMGIIVLELYWKHAPKTCKNFAELARRGYYNGTKFHRIIKDFMIQGGDPTGTGRGGASIYGKQFEDELHPDLKFTGAGILAMANAGPDTNGSQFFVTLAPTQWLDGKHTIFGRVCQGIGMVNRVGMVETNSQDRPVDDVKIIKAYPSG

Entity 20 Entity 20 polymer

Description description	;U2 small nuclear ribonucleoprotein A' ;
Molecular weight formula_weight	28484.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	W

MVKLTAELIEQAAQYTNAVRDRELDLRGYKIPVIENLGATLDQFDAIDFSDNEIRKLDGFPLLRRLKTLLVNNNRICRIGEGLDQALPDLTELILTNNSLVELGDLDPLASLKSLTYLCILRNPVTNKKHYRLYVIYKVPQVRVLDFQKVKLKERQEAEKMFKGKRGAQLAKDIARRSKTFNPGAGLPTDKKKGGPSPGDVEAIKNAIANASTLAEVERLKGLLQSGQIPGRERRSGPTDDGEEEMEEDTVTNGS

Entity 21 Entity 21 polymer

Description description	;U2 small nuclear ribonucleoprotein B'' ;
Molecular weight formula_weight	25524.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	X

MDIRPNHTIYINNMNDKIKKEELKRSLYALFSQFGHVVDIVALKTMKMRGQAFVIFKELGSSTNALRQLQGFPFYGKPMRIQYAKTDSDIISKMRGTFADKEKKKEKKKAKTVEQTATTTNKKPGQGTPNSANTQGNSTPNPQVPDYPPNYILFLNNLPEETNEMMLSMLFNQFPGFKEVRLVPGRHDIAFVEFENDGQAGAARDALQGFKITPSHAMKITYAKK

Entity 22 Entity 22 polymer

Description description	Small nuclear ribonucleoprotein Sm D2
Molecular weight formula_weight	13551.9 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	a,h

MSLLNKPKSEMTPEELQKREEEEFNTGPLSVLTQSVKNNTQVLINCRNNKKLLGRVKAFDRHCNMVLENVKEMWTEVPKSGKGKKKSKPVNKDRYISKMFLRGDSVIVVLRNPLIAGK

Entity 23 Entity 23 polymer

Description description	Small nuclear ribonucleoprotein F
Molecular weight formula_weight	9734.2 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	b,i

MSLPLNPKPFLNGLTGKPVMVKLKWGMEYKGYLVSVDGYMNMQLANTEEYIDGALSGHLGEVLIRCNNVLYIRGVEEEEEDGEMRE

Entity 24 Entity 24 polymer

Description description	Small nuclear ribonucleoprotein E
Molecular weight formula_weight	10817.6 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	c,j

MAYRGQGQKVQKVMVQPINLIFRYLQNRSRIQVWLYEQVNMRIEGCIIGFDEYMNLVLDDAEEIHSKTKSRKQLGRIMLKGDNITLLQSVSN

Entity 25 Entity 25 polymer

Description description	Small nuclear ribonucleoprotein G
Molecular weight formula_weight	8508.1 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	d,k

MSKAHPPELKKFMDKKLSLKLNGGRHVQGILRGFDPFMNLVIDECVEMATSGQQNNIGMVVIRGNSIIMLEALERV

Entity 26 Entity 26 polymer

Description description	Small nuclear ribonucleoprotein Sm D3
Molecular weight formula_weight	13940.3 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	e,l

MSIGVPIKVLHEAEGHIVTCETNTGEVYRGKLIEAEDNMNCQMSNITVTYRDGRVAQLEQVYIRGSKIRFLILPDMLKNAPMLKSMKNKNQGSGAGRGKAAILKAQVAARGRGRGMGRGNIFQKRR

Entity 27 Entity 27 polymer

Description description	;Small nuclear ribonucleoprotein-associated proteins B and B' ;
Molecular weight formula_weight	24642.1 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	f,m

MTVGKSSKMLQHIDYRMRCILQDGRIFIGTFKAFDKHMNLILCDCDEFRKIKPKNSKQAEREEKRVLGLVLLRGENLVSMTVEGPPPKDTGIARVPLAGAAGGPGIGRAAGRGIPAGVPMPQAPAGLAGPVRGVGGPSQQVMTPQGRGTVAAAAAAATASIAGAPTQYPPGRGGPPPPMGRGAPPPGMMGPPPGMRPPMGPPMGIPPGRGTPMGMPPPGMRPPPPGMRGPPPPGMRPPRP

Entity 28 Entity 28 polymer

Description description	Small nuclear ribonucleoprotein Sm D1
Molecular weight formula_weight	13310.7 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	g,n

MKLVRFLMKLSHETVTIELKNGTQVHGTITGVDVSMNTHLKAVKMTLKNREPVQLETLSIRGNNIRYFILPDSLPLDTLLVDVEPKVKSKKREAVAGRGRGRGRGRGRGRGRGRGGPRR

Entity 29 Entity 29 polymer

Description description	Peptidyl-prolyl cis-trans isomerase E
Molecular weight formula_weight	33475.8 kDa
EC 编号 ec_number	5.2.1.8
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	o

MATTKRVLYVGGLAEEVDDKVLHAAFIPFGDITDIQIPLDYETEKHRGFAFVEFELAEDAAAAIDNMNESELFGRTIRVNLAKPMRIKEGSSRPVWSDDDWLKKFSGKTLEENKEEEGSEPPKAETQEGEPIAKKARSNPQVYMDIKIGNKPAGRIQMLLRSDVVPMTAENFRCLCTHEKGFGFKGSSFHRIIPQFMCQGGDFTNHNGTGGKSIYGKKFDDENFILKHTGPGLLSMANSGPNTNGSQFFLTCDKTDWLDGKHVVFGEVTEGLDVLRQIEAQGSKDGKPKQKVIIADCGEYV

Entity 30 Entity 30 polymer

Description description	Eukaryotic initiation factor 4A-III
Molecular weight formula_weight	46931.0 kDa
EC 编号 ec_number	3.6.4.13
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	p

MATTATMATSGSARKRLLKEEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVVLISATLPHEILEMTNKFMTDPIRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI

Entity 31 Entity 31 polymer

Description description	ATP-dependent RNA helicase DHX8
Molecular weight formula_weight	139252.0 kDa
EC 编号 ec_number	3.6.4.13
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	q

MAVAVAMAGALIGSEPGPAEELAKLEYLSLVSKVCTELDNHLGINDKDLAEFVISLAEKNTTFDTFKASLVKNGAEFTDSLISNLLRLIQTMRPPAKPSTSKDPVVKPKTEKEKLKELFPVLCQPDNPSVRTMLDEDDVKVAVDVLKELEALMPSAAGQEKQRDAEHRDRTKKKKRSRSRDRNRDRDRDRERNRDRDHKRRHRSRSRSRSRTRERNKVKSRYRSRSRSQSPPKDRKDRDKYGERNLDRWRDKHVDRPPPEEPTIGDIYNGKVTSIMQFGCFVQLEGLRKRWEGLVHISELRREGRVANVADVVSKGQRVKVKVLSFTGTKTSLSMKDVDQETGEDLNPNRRRNLVGETNEETSMRNPDRPTHLSLVSAPEVEDDSLERKRLTRISDPEKWEIKQMIAANVLSKEEFPDFDEETGILPKVDDEEDEDLEIELVEEEPPFLRGHTKQSMDMSPIKIVKNPDGSLSQAAMMQSALAKERRELKQAQREAEMDSIPMGLNKHWVDPLPDAEGRQIAANMRGIGMMPNDIPEWKKHAFGGNKASYGKKTQMSILEQRESLPIYKLKEQLVQAVHDNQILIVIGETGSGKTTQITQYLAEAGYTSRGKIGCTQPRRVAAMSVAKRVSEEFGCCLGQEVGYTIRFEDCTSPETVIKYMTDGMLLRECLIDPDLTQYAIIMLDEAHERTIHTDVLFGLLKKTVQKRQDMKLIVTSATLDAVKFSQYFYEAPIFTIPGRTYPVEILYTKEPETDYLDASLITVMQIHLTEPPGDILVFLTGQEEIDTACEILYERMKSLGPDVPELIILPVYSALPSEMQTRIFDPAPPGSRKVVIATNIAETSLTIDGIYYVVDPGFVKQKVYNSKTGIDQLVVTPISQAQAKQRAGRAGRTGPGKCYRLYTERAYRDEMLTTNVPEIQRTNLASTVLSLKAMGINDLLSFDFGSPMETLITAMEQLYTLGALDDEGLLTRLGRRMAEFPLEPMLCKMLIMSVHLGCSEEMLTIVSMLSVQNVFYRPKDKQALADQKKAKFHQTEGDHLTLLAVYNSWKNNKFSNPWCYENFIQARSLRRAQDIRKQMLGIMDRHKLDVVSCGKSTVRVQKAICSGFFRNAAKKDPQEGYRTLIDQQVVYIHPSSALFNRQPEWVVYHELVLTTKEYMREVTTIDPRWLVEFAPAFFKVSDPTKLSKQKKQQRLEPLYNRYEEPNAWRISRAFRRR

Entity 32 Entity 32 polymer

Description description	MINX pre-mRNA (intron)
Molecular weight formula_weight	104019.2 kDa
Source method src_method	nat
Copy count entity_count	2

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	Y,Z

GGGAGACGGAAUUCGAGCUCGCCCACUCUUGGAUCGGAAACCCGUCGGCCUCCGAACGGUAAGAGCCUAGCAUGUAGAACUGGUUACCUGCAGCCCAAGCUUGCUGCACGUCUAGGGCGCAGUAGUCCAGGGUUUCCUUGAUGAUGUCAUACUUAUGCUGUCCCUUUUUUUUCCACAGCUCGCGGUUGAGGACAAACUCUUCGCGGUCUUUCCAGUGGGGAUCCGAUAUCCGUACACCAUCAGGGUACGAGCUAGCCCAUGGCGUACACCAUCAGGGUACGACUAGUAGAUCUCGUACACCAUCAGGGUACGGAAUUCUCUAGA

Entity 33 Entity 33 polymer

Description description	Human gene for small nuclear RNA U2 (snRNA U2)
Molecular weight formula_weight	60186.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	2

AUCGCUUCUCGGCCUUUUGGCUAAGAUCAAGUGUAGUAUCUGUUCUUAUCAGUUUAAUAUCUGAUACGUCCUCUAUCCGAGGACAAUAUAUUAAAUGGAUUUUUGGAGCAGGGAGAUGGAAUAGGAGCUUGCUCCGUCCACUCCACGCAUCGACCUGGUAUUGCAGUACCUCCAGGAACGGUGCACCC

Entity 34 Entity 34 polymer

Description description	Homo sapiens U5 A small nuclear RNA
Molecular weight formula_weight	36908.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	5

AUACUCUGGUUUCUCUUCAGAUCGCAUAAAUCUUUCGCCUUUUACUAAAGAUUUCCGUGGAGAGGAACAACUCUGAGUCUUAACCCAAUUUUUUGAGCCUUGCCUUGGCAAGGCUA

Entity 35 Entity 35 polymer

Description description	Homo sapiens RNA, U6 small nuclear 1 (RNU6-1), small nuclear RNA
Molecular weight formula_weight	34098.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	6

GUGCUCGCUUCGGCAGCACAUAUACUAAAAUUGGAACGAUACAGAGAAGAUUAGCAUGGCCCCUGCGCAAGGAUGACACGCAAAUUCGUGAAGCGUUCCAUAUUUU

▶

Resolution resolution	5.90 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	136534

Cryo-EM structure of a human spliceosome activated for step 2 of splicing (C* complex)

1. Protein Identity and Related Structures Protein Identity & Related Structures

Pre-mRNA-processing-splicing factor 8

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

116 kDa U5 small nuclear ribonucleoprotein component

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

SNW domain-containing protein 1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pleiotropic regulator 1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pre-mRNA-processing factor 17

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

U5 small nuclear ribonucleoprotein 40 kDa protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pre-mRNA-processing factor 19

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pre-mRNA-splicing factor SPF27

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Cell division cycle 5-like protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pre-mRNA-splicing factor SYF1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer