7aav

Human pre-Bact-2 spliceosome core structure

Method: ELECTRON MICROSCOPY

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1. Protein Identity and Related Structures Protein Identity & Related Structures

116 kDa U5 small nuclear ribonucleoprotein component

OrganismNot specified

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 4	Protein BUD31 homolog × 1 （P41223） Cell division cycle 5-like protein × 1 （Q99459） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Microfibrillar-associated protein 1 × 1 （P55081） Pleiotropic regulator 1 × 1 （O43660） MINX M3 pre-mRNA × 1 Pre-mRNA-processing factor 17 × 1 （O60508） Pre-mRNA-splicing factor 38A × 1 （Q8NAV1） Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） SNW domain-containing protein 1 × 1 （Q13573） Zinc finger matrin-type protein 2 × 1 （Q96NC0） U2 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 Ubiquitin-like protein 5 × 1 （Q9BZL1） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 D-chiro inositol hexakisphosphate × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	U5S1_HUMAN
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain r； PDBConstruct 1–972； UniProt 1–972

Protein BUD31 homolog

OrganismNot specified

UniProt P41223

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 4	116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） Cell division cycle 5-like protein × 1 （Q99459） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Microfibrillar-associated protein 1 × 1 （P55081） Pleiotropic regulator 1 × 1 （O43660） MINX M3 pre-mRNA × 1 Pre-mRNA-processing factor 17 × 1 （O60508） Pre-mRNA-splicing factor 38A × 1 （Q8NAV1） Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） SNW domain-containing protein 1 × 1 （Q13573） Zinc finger matrin-type protein 2 × 1 （Q96NC0） U2 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 Ubiquitin-like protein 5 × 1 （Q9BZL1） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 D-chiro inositol hexakisphosphate × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	BUD31_HUMAN
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain Q； PDBConstruct 1–144； UniProt 1–144

Cell division cycle 5-like protein

OrganismNot specified

UniProt Q99459

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 4	116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） Protein BUD31 homolog × 1 （P41223） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Microfibrillar-associated protein 1 × 1 （P55081） Pleiotropic regulator 1 × 1 （O43660） MINX M3 pre-mRNA × 1 Pre-mRNA-processing factor 17 × 1 （O60508） Pre-mRNA-splicing factor 38A × 1 （Q8NAV1） Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） SNW domain-containing protein 1 × 1 （Q13573） Zinc finger matrin-type protein 2 × 1 （Q96NC0） U2 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 Ubiquitin-like protein 5 × 1 （Q9BZL1） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 D-chiro inositol hexakisphosphate × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CDC5L_HUMAN
Isoform	—
PDB entities	3
Chains and sequence ranges	Author chain L； PDBConstruct 1–802； UniProt 1–802

Spliceosome-associated protein CWC15 homolog

OrganismNot specified

UniProt Q9P013

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 4	116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） Protein BUD31 homolog × 1 （P41223） Cell division cycle 5-like protein × 1 （Q99459） Microfibrillar-associated protein 1 × 1 （P55081） Pleiotropic regulator 1 × 1 （O43660） MINX M3 pre-mRNA × 1 Pre-mRNA-processing factor 17 × 1 （O60508） Pre-mRNA-splicing factor 38A × 1 （Q8NAV1） Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） SNW domain-containing protein 1 × 1 （Q13573） Zinc finger matrin-type protein 2 × 1 （Q96NC0） U2 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 Ubiquitin-like protein 5 × 1 （Q9BZL1） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 D-chiro inositol hexakisphosphate × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	CWC15_HUMAN
Isoform	—
PDB entities	4
Chains and sequence ranges	Author chain R； PDBConstruct 1–229； UniProt 1–229

Microfibrillar-associated protein 1

OrganismNot specified

UniProt P55081

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 4	116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） Protein BUD31 homolog × 1 （P41223） Cell division cycle 5-like protein × 1 （Q99459） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Pleiotropic regulator 1 × 1 （O43660） MINX M3 pre-mRNA × 1 Pre-mRNA-processing factor 17 × 1 （O60508） Pre-mRNA-splicing factor 38A × 1 （Q8NAV1） Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） SNW domain-containing protein 1 × 1 （Q13573） Zinc finger matrin-type protein 2 × 1 （Q96NC0） U2 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 Ubiquitin-like protein 5 × 1 （Q9BZL1） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 D-chiro inositol hexakisphosphate × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	MFAP1_HUMAN
Isoform	—
PDB entities	5
Chains and sequence ranges	Author chain K； PDBConstruct 1–439； UniProt 1–439

Pleiotropic regulator 1

OrganismNot specified

UniProt O43660

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 4	116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） Protein BUD31 homolog × 1 （P41223） Cell division cycle 5-like protein × 1 （Q99459） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Microfibrillar-associated protein 1 × 1 （P55081） MINX M3 pre-mRNA × 1 Pre-mRNA-processing factor 17 × 1 （O60508） Pre-mRNA-splicing factor 38A × 1 （Q8NAV1） Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） SNW domain-containing protein 1 × 1 （Q13573） Zinc finger matrin-type protein 2 × 1 （Q96NC0） U2 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 Ubiquitin-like protein 5 × 1 （Q9BZL1） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 D-chiro inositol hexakisphosphate × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PLRG1_HUMAN
Isoform	—
PDB entities	6
Chains and sequence ranges	Author chain G； PDBConstruct 1–514； UniProt 1–514

Pre-mRNA-processing factor 17

OrganismNot specified

UniProt O60508

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 4	116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） Protein BUD31 homolog × 1 （P41223） Cell division cycle 5-like protein × 1 （Q99459） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Microfibrillar-associated protein 1 × 1 （P55081） Pleiotropic regulator 1 × 1 （O43660） MINX M3 pre-mRNA × 1 Pre-mRNA-splicing factor 38A × 1 （Q8NAV1） Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） SNW domain-containing protein 1 × 1 （Q13573） Zinc finger matrin-type protein 2 × 1 （Q96NC0） U2 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 Ubiquitin-like protein 5 × 1 （Q9BZL1） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 D-chiro inositol hexakisphosphate × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PRP17_HUMAN
Isoform	—
PDB entities	8
Chains and sequence ranges	Author chain 8； PDBConstruct 1–579； UniProt 1–579

Pre-mRNA-splicing factor 38A

OrganismNot specified

UniProt Q8NAV1

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 4	116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） Protein BUD31 homolog × 1 （P41223） Cell division cycle 5-like protein × 1 （Q99459） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Microfibrillar-associated protein 1 × 1 （P55081） Pleiotropic regulator 1 × 1 （O43660） MINX M3 pre-mRNA × 1 Pre-mRNA-processing factor 17 × 1 （O60508） Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） SNW domain-containing protein 1 × 1 （Q13573） Zinc finger matrin-type protein 2 × 1 （Q96NC0） U2 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 Ubiquitin-like protein 5 × 1 （Q9BZL1） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 D-chiro inositol hexakisphosphate × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PR38A_HUMAN
Isoform	—
PDB entities	9
Chains and sequence ranges	Author chain I； PDBConstruct 1–312； UniProt 1–312

Pre-mRNA-processing-splicing factor 8

OrganismNot specified

UniProt Q6P2Q9

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 4	116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） Protein BUD31 homolog × 1 （P41223） Cell division cycle 5-like protein × 1 （Q99459） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Microfibrillar-associated protein 1 × 1 （P55081） Pleiotropic regulator 1 × 1 （O43660） MINX M3 pre-mRNA × 1 Pre-mRNA-processing factor 17 × 1 （O60508） Pre-mRNA-splicing factor 38A × 1 （Q8NAV1） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） SNW domain-containing protein 1 × 1 （Q13573） Zinc finger matrin-type protein 2 × 1 （Q96NC0） U2 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 Ubiquitin-like protein 5 × 1 （Q9BZL1） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 D-chiro inositol hexakisphosphate × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	PRP8_HUMAN
Isoform	—
PDB entities	10
Chains and sequence ranges	Author chain A； PDBConstruct 1–2335； UniProt 1–2335

Pre-mRNA-splicing factor RBM22

OrganismNot specified

UniProt Q9NW64

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 4	116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） Protein BUD31 homolog × 1 （P41223） Cell division cycle 5-like protein × 1 （Q99459） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Microfibrillar-associated protein 1 × 1 （P55081） Pleiotropic regulator 1 × 1 （O43660） MINX M3 pre-mRNA × 1 Pre-mRNA-processing factor 17 × 1 （O60508） Pre-mRNA-splicing factor 38A × 1 （Q8NAV1） Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） SNW domain-containing protein 1 × 1 （Q13573） Zinc finger matrin-type protein 2 × 1 （Q96NC0） U2 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 Ubiquitin-like protein 5 × 1 （Q9BZL1） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 D-chiro inositol hexakisphosphate × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	RBM22_HUMAN
Isoform	—
PDB entities	11
Chains and sequence ranges	Author chain P； PDBConstruct 1–420； UniProt 1–420

SNW domain-containing protein 1

OrganismNot specified

UniProt Q13573

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 4	116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） Protein BUD31 homolog × 1 （P41223） Cell division cycle 5-like protein × 1 （Q99459） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Microfibrillar-associated protein 1 × 1 （P55081） Pleiotropic regulator 1 × 1 （O43660） MINX M3 pre-mRNA × 1 Pre-mRNA-processing factor 17 × 1 （O60508） Pre-mRNA-splicing factor 38A × 1 （Q8NAV1） Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） Zinc finger matrin-type protein 2 × 1 （Q96NC0） U2 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 Ubiquitin-like protein 5 × 1 （Q9BZL1） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 D-chiro inositol hexakisphosphate × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	SNW1_HUMAN
Isoform	—
PDB entities	12
Chains and sequence ranges	Author chain v； PDBConstruct 1–536； UniProt 1–536

Zinc finger matrin-type protein 2

OrganismNot specified

UniProt Q96NC0

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 4	116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） Protein BUD31 homolog × 1 （P41223） Cell division cycle 5-like protein × 1 （Q99459） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Microfibrillar-associated protein 1 × 1 （P55081） Pleiotropic regulator 1 × 1 （O43660） MINX M3 pre-mRNA × 1 Pre-mRNA-processing factor 17 × 1 （O60508） Pre-mRNA-splicing factor 38A × 1 （Q8NAV1） Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） SNW domain-containing protein 1 × 1 （Q13573） U2 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 Ubiquitin-like protein 5 × 1 （Q9BZL1） GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 D-chiro inositol hexakisphosphate × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	ZMAT2_HUMAN
Isoform	—
PDB entities	13
Chains and sequence ranges	Author chain N； PDBConstruct 1–199； UniProt 1–199

Ubiquitin-like protein 5

OrganismNot specified

UniProt Q9BZL1

State in the Current Structure

Assembly	Physical composition	Protein state	Molecular copy count	Associated components	Data consistency
1	Protein–RNA	Heteromer	Protein 13 RNA 4	116 kDa U5 small nuclear ribonucleoprotein component × 1 （Q15029） Protein BUD31 homolog × 1 （P41223） Cell division cycle 5-like protein × 1 （Q99459） Spliceosome-associated protein CWC15 homolog × 1 （Q9P013） Microfibrillar-associated protein 1 × 1 （P55081） Pleiotropic regulator 1 × 1 （O43660） MINX M3 pre-mRNA × 1 Pre-mRNA-processing factor 17 × 1 （O60508） Pre-mRNA-splicing factor 38A × 1 （Q8NAV1） Pre-mRNA-processing-splicing factor 8 × 1 （Q6P2Q9） Pre-mRNA-splicing factor RBM22 × 1 （Q9NW64） SNW domain-containing protein 1 × 1 （Q13573） Zinc finger matrin-type protein 2 × 1 （Q96NC0） U2 snRNA × 1 U5 snRNA × 1 U6 snRNA × 1 GUANOSINE-5'-TRIPHOSPHATE × 1 MAGNESIUM ION × 1 D-chiro inositol hexakisphosphate × 1	Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence

UniProt name	UBL5_HUMAN
Isoform	—
PDB entities	17
Chains and sequence ranges	Author chain q； PDBConstruct 1–73； UniProt 1–73

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	7aav
Deposition date deposition_date	2020-09-04
Structure title title	Human pre-Bact-2 spliceosome core structure
Keywords keywords	Complex, spliceosome, catalytic activation, splicing; SPLICING
Experimental Method method	ELECTRON MICROSCOPY

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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

7aav__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

7aav__assembly_1__model_1 | I(q)

100 plotted points; both axes use logarithmic scales.

7aav__assembly_1__model_1 | P(r) · Pending

P(r) will be calculated and displayed separately for the same assembly/model.

Rg（Guinier）	53.74 Å
Rg (electron density)	53.62 Å
Total Rg	53.62 Å
Atom count	25867
Residues	4151
Excluded volume	421440 Å³
Maximum q	0.500 Å⁻¹

Assembly	Model	Structure unit	Oligomeric description	Status	CRYSOL	Actions
1	1	`7aav__assembly_1__model_1`	heptadecameric （17）	Success	4.1.3-1-20251215 (887e7ef)	View Download

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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (20)

Entity 1 Entity 1 polymer

Description description	116 kDa U5 small nuclear ribonucleoprotein component
Molecular weight formula_weight	109560.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	r
Atom count atom_count	25867

MDTDLYDEFGNYIGPELDSDEDDDELGRETKDLDEMDDDDDDDDVGDHDDDHPGMEVVLHEDKKYYPTAEEVYGPEVETIVQEEDTQPLTEPIIKPVKTKKFTLMEQTLPVTVYEMDFLADLMDNSELIRNVTLCGHLHHGKTCFVDCLIEQTHPEIRKRYDQDLCYTDILFTEQERGVGIKSTPVTVVLPDTKGKSYLFNIMDTPGHVNFSDEVTAGLRISDGVVLFIDAAEGVMLNTERLIKHAVQERLAVTVCINKIDRLILELKLPPTDAYYKLRHIVDEVNGLISMYSTDENLILSPLLGNVCFSSSQYSICFTLGSFAKIYADTFGDINYQEFAKRLWGDIYFNPKTRKFTKKAPTSSSQRSFVEFILEPLYKILAQVVGDVDTSLPRTLDELGIHLTKEELKLNIRPLLRLVCKKFFGEFTGFVDMCVQHIPSPKVGAKPKIEHTYTGGVDSDLGEAMSDCDPDGPLMCHTTKMYSTDDGVQFHAFGRVLSGTIHAGQPVKVLGENYTLEDEEDSQICTVGRLWISVARYHIEVNRVPAGNWVLIEGVDQPIVKTATITEPRGNEEAQIFRPLKFNTTSVIKIAVEPVNPSELPKMLDGLRKVNKSYPSLTTKVEESGEHVILGTGELYLDCVMHDLRKMYSEIDIKVADPVVTFCETVVETSSLKCFAETPNKKNKITMIAEPLEKGLAEDIENEVVQITWNRKKLGEFFQTKYDWDLLAARSIWAFGPDATGPNILVDDTLPSEVDKALLGSVKDSIVQGFQWGTREGPLCDELIRNVKFKILDAVVAQEPLHRGGGQIIPTARRVVYSAFLMATPRLMEPYYFVEVQAPADCVSAVYTVLARRRGHVTQDAPIPGSPLYTIKAFIPAIDSFGFETDLRTHTQGQAFSLSVFHHWQIVPGDPLDKSIVIRPLEPQPAPHLAREFMIKTRRRKGLSEDVSISKFFDDPMLLELAKQDVVLNYPM

Entity 2 Entity 2 polymer

Description description	Protein BUD31 homolog
Molecular weight formula_weight	17032.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	Q

MPKVKRSRKAPPDGWELIEPTLDELDQKMREAETEPHEGKRKVESLWPIFRIHHQKTRYIFDLFYKRKAISRELYEYCIKEGYADKNLIAKWKKQGYENLCCLRCIQTRDTNFGTNCICRVPKSKLEVGRIIECTHCGCRGCSG

Entity 3 Entity 3 polymer

Description description	Cell division cycle 5-like protein
Molecular weight formula_weight	92406.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	L

MPRIMIKGGVWRNTEDEILKAAVMKYGKNQWSRIASLLHRKSAKQCKARWYEWLDPSIKKTEWSREEEEKLLHLAKLMPTQWRTIAPIIGRTAAQCLEHYEFLLDKAAQRDNEEETTDDPRKLKPGEIDPNPETKPARPDPIDMDEDELEMLSEARARLANTQGKKAKRKAREKQLEEARRLAALQKRRELRAAGIEIQKKRKRKRGVDYNAEIPFEKKPALGFYDTSEENYQALDADFRKLRQQDLDGELRSEKEGRDRKKDKQHLKRKKESDLPSAILQTSGVSEFTKKRSKLVLPAPQISDAELQEVVKVGQASEIARQTAEESGITNSASSTLLSEYNVTNNSVALRTPRTPASQDRILQEAQNLMALTNVDTPLKGGLNTPLHESDFSGVTPQRQVVQTPNTVLSTPFRTPSNGAEGLTPRSGTTPKPVINSTPGRTPLRDKLNINPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQEREKELQHRYADLLLEKETLKSKF

Entity 4 Entity 4 polymer

Description description	Spliceosome-associated protein CWC15 homolog
Molecular weight formula_weight	26674.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	R

MTTAARPTFEPARGGRGKGEGDLSQLSKQYSSRDLPSHTKIKYRQTTQDAPEEVRNRDFRRELEERERAAAREKNRDRPTREHTTSSSVSKKPRLDQIPAANLDADDPLTDEEDEDFEEESDDDDTAALLAELEKIKKERAEEQARKEQEQKAEEERIRMENILSGNPLLNLTGPSQPQANFKVKRRWDDDVVFKNCAKGVDDQKKDKRFVNDTLRSEFHKKFMEKYIK

Entity 5 Entity 5 polymer

Description description	Microfibrillar-associated protein 1
Molecular weight formula_weight	52050.5 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	K

MSVPSALMKQPPIQSTAGAVPVRNEKGEISMEKVKVKRYVSGKRPDYAPMESSDEEDEEFQFIKKAKEQEAEPEEQEEDSSSDPRLRRLQNRISEDVEERLARHRKIVEPEVVGESDSEVEGDAWRMEREDSSEEEEEEIDDEEIERRRGMMRQRAQERKNEEMEVMEVEDEGRSGEESESESEYEEYTDSEDEMEPRLKPVFIRKKDRVTVQEREAEALKQKELEQEAKRMAEERRKYTLKIVEEETKKELEENKRSLAALDALNTDDENDEEEYEAWKVRELKRIKRDREDREALEKEKAEIERMRNLTEEERRAELRANGKVITNKAVKGKYKFLQKYYHRGAFFMDEDEEVYKRDFSAPTLEDHFNKTILPKVMQVKNFGRSGRTKYTHLVDQDTTSFDSAWGQESAQNTKFFKQKAAGVRDVFERPSAKKRKTT

Entity 6 Entity 6 polymer

Description description	Pleiotropic regulator 1
Molecular weight formula_weight	57280.8 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	G

MVEEVQKHSVHTLVFRSLKRTHDMFVADNGKPVPLDEESHKRKMAIKLRNEYGPVLHMPTSKENLKEKGPQNATDSYVHKQYPANQGQEVEYFVAGTHPYPPGPGVALTADTKIQRMPSESAAQSLAVALPLQTKADANRTAPSGSEYRHPGASDRPQPTAMNSIVMETGNTKNSALMAKKAPTMPKPQWHPPWKLYRVISGHLGWVRCIAVEPGNQWFVTGSADRTIKIWDLASGKLKLSLTGHISTVRGVIVSTRSPYLFSCGEDKQVKCWDLEYNKVIRHYHGHLSAVYGLDLHPTIDVLVTCSRDSTARIWDVRTKASVHTLSGHTNAVATVRCQAAEPQIITGSHDTTIRLWDLVAGKTRVTLTNHKKSVRAVVLHPRHYTFASGSPDNIKQWKFPDGSFIQNLSGHNAIINTLTVNSDGVLVSGADNGTMHLWDWRTGYNFQRVHAAVQPGSLDSESGIFACAFDQSESRLLTAEADKTIKVYREDDTATEETHPVSWKPEIIKRKRF

Entity 7 Entity 7 polymer

Description description	MINX M3 pre-mRNA
Molecular weight formula_weight	73712.4 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	Z

GGGAGACGGAAUUCGAGCUCGCCCACUCUUGGAUCGGAAACCCGUCGGCCUCCGAACGGUAAGAGCCUAGCAUGUAGAACUGGUUACCUGCAGCCCAAGCUUGCUGCACGUCUAGGGCGCAGUAGUCCAGGGUUUCCUUGAUGAUGUCAUACUUAUCCUGUCCCUUUUUUUUCCACAGCUCGCGGUUGAGGACAAACUCUUCGCGGUCUUUCCAGUGGGGAUCCAAUAUC

Entity 8 Entity 8 polymer

Description description	Pre-mRNA-processing factor 17
Molecular weight formula_weight	65612.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	8

MSAAIAALAASYGSGSGSESDSDSESSRCPLPAADSLMHLTKSPSSKPSLAVAVDSAPEVAVKEDLETGVHLDPAVKEVQYNPTYETMFAPEFGPENPFRTQQMAAPRNMLSGYAEPAHINDFMFEQQRRTFATYGYALDPSLDNHQVSAKYIGSVEEAEKNQGLTVFETGQKKTEKRKKFKENDASNIDGFLGPWAKYVDEKDVAKPSEEEQKELDEITAKRQKKGKQEEEKPGEEKTILHVKEMYDYQGRSYLHIPQDVGVNLRSTMPPEKCYLPKKQIHVWSGHTKGVSAVRLFPLSGHLLLSCSMDCKIKLWEVYGERRCLRTFIGHSKAVRDICFNTAGTQFLSAAYDRYLKLWDTETGQCISRFTNRKVPYCVKFNPDEDKQNLFVAGMSDKKIVQWDIRSGEIVQEYDRHLGAVNTIVFVDENRRFVSTSDDKSLRVWEWDIPVDFKYIAEPSMHSMPAVTLSPNGKWLACQSMDNQILIFGAQNRFRLNKKKIFKGHMVAGYACQVDFSPDMSYVISGDGNGKLNIWDWKTTKLYSRFKAHDKVCIGAVWHPHETSKVITCGWDGLIKLWD

Entity 9 Entity 9 polymer

Description description	Pre-mRNA-splicing factor 38A
Molecular weight formula_weight	37563.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	I

MANRTVKDAHSIHGTNPQYLVEKIIRTRIYESKYWKEECFGLTAELVVDKAMELRFVGGVYGGNIKPTPFLCLTLKMLQIQPEKDIIVEFIKNEDFKYVRMLGALYMRLTGTAIDCYKYLEPLYNDYRKIKSQNRNGEFELMHVDEFIDELLHSERVCDIILPRLQKRYVLEEAEQLEPRVSALEEDMDDVESSEEEEEEDEKLERVPSPDHRRRSYRDLDKPRRSPTLRYRRSRSRSPRRRSRSPKRRSPSPRRERHRSKSPRRHRSRSRDRRHRSRSKSPGHHRSHRHRSHSKSPERSKKSHKKSRRGNE

Entity 10 Entity 10 polymer

Description description	Pre-mRNA-processing-splicing factor 8
Molecular weight formula_weight	273974.2 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A

MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Entity 11 Entity 11 polymer

Description description	Pre-mRNA-splicing factor RBM22
Molecular weight formula_weight	46959.6 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	P

MATSLGSNTYNRQNWEDADFPILCQTCLGENPYIRMTKEKYGKECKICARPFTVFRWCPGVRMRFKKTEVCQTCSKLKNVCQTCLLDLEYGLPIQVRDAGLSFKDDMPKSDVNKEYYTQNMEREISNSDGTRPVGMLGKATSTSDMLLKLARTTPYYKRNRPHICSFWVKGECKRGEECPYRHEKPTDPDDPLADQNIKDRYYGINDPVADKLLKRASTMPRLDPPEDKTITTLYVGGLGDTITETDLRNHFYQFGEIRTITVVQRQQCAFIQFATRQAAEVAAEKSFNKLIVNGRRLNVKWGRSQAARGKEKEKDGTTDSGIKLEPVPGLPGALPPPPAAEEEASANYFNLPPSGPPAVVNIALPPPPGIAPPPPPGFGPHMFHPMGPPPPFMRAPGPIHYPSQDPQRMGAHAGKHSSP

Entity 12 Entity 12 polymer

Description description	SNW domain-containing protein 1
Molecular weight formula_weight	61610.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	v

MALTSFLPAPTQLSQDQLEAEEKARSQRSRQTSLVSSRREPPPYGYRKGWIPRLLEDFGDGGAFPEIHVAQYPLDMGRKKKMSNALAIQVDSEGKIKYDAIARQGQSKDKVIYSKYTDLVPKEVMNADDPDLQRPDEEAIKEITEKTRVALEKSVSQKVAAAMPVRAADKLAPAQYIRYTPSQQGVAFNSGAKQRVIRMVEMQKDPMEPPRFKINKKIPRGPPSPPAPVMHSPSRKMTVKEQQEWKIPPCISNWKNAKGYTIPLDKRLAADGRGLQTVHINENFAKLAEALYIADRKAREAVEMRAQVERKMAQKEKEKHEEKLREMAQKARERRAGIKTHVEKEDGEARERDEIRHDRRKERQHDRNLSRAAPDKRSKLQRNENRDISEVIALGVPNPRTSNEVQYDQRLFNQSKGMDSGFAGGEDEIYNVYDQAWRGGKDMAQSIYRPSKNLDKDMYGDDLEARIKTNRFVPDKEFSGSDRRQRGREGPVQFEEDPFGLDKFLEEAKQHGGSKRPSDSSRPKEHEHEGKKRRKE

Entity 13 Entity 13 polymer

Description description	Zinc finger matrin-type protein 2
Molecular weight formula_weight	23664.0 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	N

MASGSGTKNLDFRRKWDKDEYEKLAEKRLTEEREKKDGKPVQPVKRELLRHRDYKVDLESKLGKTIVITKTTPQSEMGGYYCNVCDCVVKDSINFLDHINGKKHQRNLGMSMRVERSTLDQVKKRFEVNKKKMEEKQKDYDFEERMKELREEEEKAKAYKKEKQKEKKRRAEEDLTFEEDDEMAAVMGFSGFGSTKKSY

Entity 14 Entity 14 polymer

Description description	U2 snRNA
Molecular weight formula_weight	60186.4 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	2

AUCGCUUCUCGGCCUUUUGGCUAAGAUCAAGUGUAGUAUCUGUUCUUAUCAGUUUAAUAUCUGAUACGUCCUCUAUCCGAGGACAAUAUAUUAAAUGGAUUUUUGGAGCAGGGAGAUGGAAUAGGAGCUUGCUCCGUCCACUCCACGCAUCGACCUGGUAUUGCAGUACCUCCAGGAACGGUGCACCC

Entity 15 Entity 15 polymer

Description description	U5 snRNA
Molecular weight formula_weight	36908.7 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	5

AUACUCUGGUUUCUCUUCAGAUCGCAUAAAUCUUUCGCCUUUUACUAAAGAUUUCCGUGGAGAGGAACAACUCUGAGUCUUAACCCAAUUUUUUGAGCCUUGCCUUGGCAAGGCUA

Entity 16 Entity 16 polymer

Description description	U6 snRNA
Molecular weight formula_weight	34098.3 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polyribonucleotide
Chain IDs strand_id	6

GUGCUCGCUUCGGCAGCACAUAUACUAAAAUUGGAACGAUACAGAGAAGAUUAGCAUGGCCCCUGCGCAAGGAUGACACGCAAAUUCGUGAAGCGUUCCAUAUUUU

Entity 17 Entity 17 polymer

Description description	Ubiquitin-like protein 5
Molecular weight formula_weight	8560.9 kDa
Source method src_method	nat
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	q

MIEVVCNDRLGKKVRVKCNTDDTIGDLKKLIAAQTGTRWNKIVLKKWYTIFKDHVSLGDYEIHDGMNLELYYQ

Entity 18 Entity 18 non-polymer

Description description	GUANOSINE-5'-TRIPHOSPHATE
Molecular weight formula_weight	523.2 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 19 Entity 19 non-polymer

Description description	MAGNESIUM ION
Molecular weight formula_weight	24.3 kDa
Source method src_method	syn
Copy count entity_count	1

Entity 20 Entity 20 non-polymer

Description description	D-chiro inositol hexakisphosphate
Molecular weight formula_weight	660.0 kDa
Source method src_method	syn
Copy count entity_count	1

▶

Resolution resolution	4.20 Å
Resolution测定Method resolution_method	—
颗粒数 num_particles	39336

Human pre-Bact-2 spliceosome core structure

1. Protein Identity and Related Structures Protein Identity & Related Structures

116 kDa U5 small nuclear ribonucleoprotein component

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Protein BUD31 homolog

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Cell division cycle 5-like protein

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Spliceosome-associated protein CWC15 homolog

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Microfibrillar-associated protein 1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pleiotropic regulator 1

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pre-mRNA-processing factor 17

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pre-mRNA-splicing factor 38A

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pre-mRNA-processing-splicing factor 8

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer

Other homooligomers

Heteromeric or nucleic-acid complex

Pre-mRNA-splicing factor RBM22

State in the Current Structure

Other States of the Same Protein in the Database

Monomer

Homodimer

Homotetramer