|
3E9L
Crystal Structure of Human Prp8, Residues 1755-2016
Deposited 2008-08-22
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1760–2016（257 aa）
Fragment：endonuclease domain
|
Not recorded
|
NA SODIUM ION × 2
CL CHLORIDE ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；298 K；pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.95 Å
R-free 0.248
|
|
3ENB
Crystal Structure of PRP8 core domain IV
Deposited 2008-09-25
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1769–1990（222 aa）
Fragment：UNP residues 1769-1990
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；2.5 M NaCl, 100 mM Tris-HCl pH 7.0, 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 1.85 Å
R-free 0.243
|
|
3ENB
Crystal Structure of PRP8 core domain IV
Deposited 2008-09-25
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
1769–1990（222 aa）
Fragment：UNP residues 1769-1990
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；296 K；2.5 M NaCl, 100 mM Tris-HCl pH 7.0, 100 mM MgCl2, VAPOR DIFFUSION, HANGING DROP, temperature 296K
|
Resolution 1.85 Å
R-free 0.243
|
|
3JCR
3D structure determination of the human*U4/U6.U5* tri-snRNP complex
Deposited 2016-01-21
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 33
PDB declaration: 36-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
20 mM HEPES, pH 7.9, 100 mM KCl, 5 mM MgCl2, 0.1 mM EDTA；pH 7.9；20 mM HEPES, pH 7.9, 100 mM KCl, 5 mM MgCl2, 0.1 mM EDTA
cryo-EM vitrification conditions
Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 7.00 Å
|
|
3LRU
hPRP8 Non-Native Subdomain
Deposited 2010-02-11
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein homooligomer
Homooligomer；Protein × 2
PDB declaration: dimeric
|
Chain A
1831–1990（160 aa）
Fragment：UNP residues 1831-1990
Chain B
1831–1990（160 aa）
Fragment：UNP residues 1831-1990
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
vapour diffusion；pH 6；298 K；12% PEG4000, Tris Ph8, 1,3 diamino-propane, pH 6, vapour diffusion, temperature 298K
|
Resolution 1.85 Å
R-free 0.254
|
|
4JK7
Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1769–1990（222 aa）
Fragment：PRP8 RNase H-like domain, UNP residues 1769-1990
|
Not recorded
|
GOL GLYCEROL × 2
CL CHLORIDE ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, VAPOR DIFFUSION, temperature 298K
|
Resolution 1.40 Å
R-free 0.177
|
|
4JK7
Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
1769–1990（222 aa）
Fragment：PRP8 RNase H-like domain, UNP residues 1769-1990
|
Not recorded
|
CL CHLORIDE ION × 2
MG MAGNESIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, VAPOR DIFFUSION, temperature 298K
|
Resolution 1.40 Å
R-free 0.177
|
|
4JK8
Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1769–1990（222 aa）
Fragment：PRP8 RNase H-like domain, UNP residues 1769-1990
|
Mutation：R1865A
|
GOL GLYCEROL × 2
CL CHLORIDE ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.15 Å
R-free 0.160
|
|
4JK8
Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
1769–1990（222 aa）
Fragment：PRP8 RNase H-like domain, UNP residues 1769-1990
|
Mutation：R1865A
|
CL CHLORIDE ION × 3
MG MAGNESIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.15 Å
R-free 0.160
|
|
4JK9
Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Co ion
Deposited 2013-03-09
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1769–1990（222 aa）
Fragment：PRP8 RNase H-like domain, UNP residues 1769-1990
|
Not recorded
|
CO COBALT (II) ION × 2
GOL GLYCEROL × 1
CL CHLORIDE ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.50 Å
R-free 0.205
|
|
4JK9
Open and closed forms of wild-type human PRP8 RNase H-like domain with bound Co ion
Deposited 2013-03-09
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
1769–1990（222 aa）
Fragment：PRP8 RNase H-like domain, UNP residues 1769-1990
|
Not recorded
|
CO COBALT (II) ION × 4
CL CHLORIDE ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.50 Å
R-free 0.205
|
|
4JKA
Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Co ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1769–1990（222 aa）
Fragment：PRP8 RNase H-like domain, UNP residues 1769-1990
|
Mutation：R1865A
|
CO COBALT (II) ION × 3
CL CHLORIDE ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.32 Å
R-free 0.166
|
|
4JKA
Open and closed forms of R1865A human PRP8 RNase H-like domain with bound Co ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
1769–1990（222 aa）
Fragment：PRP8 RNase H-like domain, UNP residues 1769-1990
|
Mutation：R1865A
|
CO COBALT (II) ION × 3
CL CHLORIDE ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.32 Å
R-free 0.166
|
|
4JKB
Open and closed forms of V1788D human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1769–1990（222 aa）
Fragment：PRP8 RNase H-like domain, UNP residues 1769-1990
|
Mutation：V1788D
|
GOL GLYCEROL × 2
CL CHLORIDE ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.30 Å
R-free 0.145
|
|
4JKB
Open and closed forms of V1788D human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
1769–1990（222 aa）
Fragment：PRP8 RNase H-like domain, UNP residues 1769-1990
|
Mutation：V1788D
|
GOL GLYCEROL × 1
CL CHLORIDE ION × 2
MG MAGNESIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.30 Å
R-free 0.145
|
|
4JKC
Open and closed forms of T1800E human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1769–1990（222 aa）
Fragment：;PRP8 RNase H-like domain, UNP residues 1769-1990'
;
|
Mutation：T1800E
|
CL CHLORIDE ION × 2
GOL GLYCEROL × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.50 Å
R-free 0.164
|
|
4JKC
Open and closed forms of T1800E human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
1769–1990（222 aa）
Fragment：;PRP8 RNase H-like domain, UNP residues 1769-1990'
;
|
Mutation：T1800E
|
CL CHLORIDE ION × 2
MG MAGNESIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.50 Å
R-free 0.164
|
|
4JKD
Open and closed forms of I1790Y human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1769–1990（222 aa）
Fragment：;PRP8 RNase H-like domain, UNP residues 1769-1990'
;
|
Mutation：I1790Y
|
MG MAGNESIUM ION × 1
CL CHLORIDE ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.55 Å
R-free 0.188
|
|
4JKD
Open and closed forms of I1790Y human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
1769–1990（222 aa）
Fragment：;PRP8 RNase H-like domain, UNP residues 1769-1990'
;
|
Mutation：I1790Y
|
MG MAGNESIUM ION × 1
CL CHLORIDE ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.55 Å
R-free 0.188
|
|
4JKE
Open and closed forms of T1789P human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1769–1990（222 aa）
Fragment：;PRP8 RNase H-like domain, UNP residues 1769-1990'
;
|
Mutation：T1789P
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.65 Å
R-free 0.203
|
|
4JKE
Open and closed forms of T1789P human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
1769–1990（222 aa）
Fragment：;PRP8 RNase H-like domain, UNP residues 1769-1990'
;
|
Mutation：T1789P
|
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.65 Å
R-free 0.203
|
|
4JKF
Open and closed forms of T1791P+R1865A human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1769–1990（222 aa）
Fragment：;PRP8 RNase H-like domain, UNP residues 1769-1990'
;
|
Mutation：T1791P R1865A
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.95 Å
R-free 0.226
|
|
4JKF
Open and closed forms of T1791P+R1865A human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
1769–1990（222 aa）
Fragment：;PRP8 RNase H-like domain, UNP residues 1769-1990'
;
|
Mutation：T1791P R1865A
|
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.95 Å
R-free 0.226
|
|
4JKG
Open and closed forms of mixed T1789P+R1865A and R1865A human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1769–1990（222 aa）
Fragment：PRP8 RNase H-like domain, UNP residues 1769-1990
|
Mutation：R1865A
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.80 Å
R-free 0.234
|
|
4JKG
Open and closed forms of mixed T1789P+R1865A and R1865A human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
1769–1990（222 aa）
Fragment：PRP8 RNase H-like domain, UNP residues 1769-1990
|
Mutation：T1789P, R1865A
|
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.80 Å
R-free 0.234
|
|
4JKH
Open and closed forms of D1781E human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain A
1769–1990（222 aa）
Fragment：;PRP8 RNase H-like domain, UNP residues 1769-1990'
;
|
Mutation：D1781E
|
CL CHLORIDE ION × 2
GOL GLYCEROL × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.80 Å
R-free 0.219
|
|
4JKH
Open and closed forms of D1781E human PRP8 RNase H-like domain with bound Mg ion
Deposited 2013-03-09
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein monomer
Monomer；Protein × 1
PDB declaration: monomeric
|
Chain B
1769–1990（222 aa）
Fragment：;PRP8 RNase H-like domain, UNP residues 1769-1990'
;
|
Mutation：D1781E
|
CL CHLORIDE ION × 2
MG MAGNESIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.5；298 K；15% PEG 4000, 300mM MgCl2, 100mM Tris, pH 7.5, vapor diffusion, temperature 298K
|
Resolution 1.80 Å
R-free 0.219
|
|
4KIT
Crystal structure of human Brr2 in complex with the Prp8 Jab1/MPN domain
Deposited 2013-05-02
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain C
2064–2335（272 aa）
Fragment：Jab1/MPN domain (UNP residues 2064-2335)
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 2
MG MAGNESIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.9；277 K；0.1 M HEPES-NaOH, pH 7.9, 0.2 M magnesium chloride, 12% ethanol, Silver Bullets Bio condition 91 (Hampton Research) as additive, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 3.60 Å
R-free 0.235
|
|
5MQF
Cryo-EM structure of a human spliceosome activated for step 2 of splicing (C* complex)
Deposited 2016-12-20
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 41
PDB declaration: 41-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.90 Å
|
|
5O9Z
Cryo-EM structure of a pre-catalytic human spliceosome primed for activation (B complex)
Deposited 2017-06-20
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 52
PDB declaration: 57-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å
|
|
5XJC
Cryo-EM structure of the human spliceosome just prior to exon ligation at 3.6 angstrom
Deposited 2017-04-30
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 46
PDB declaration: 50-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 11
ADP ADENOSINE-5'-DIPHOSPHATE × 2
ZN ZINC ION × 7
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å
|
|
5YZG
The Cryo-EM Structure of Human Catalytic Step I Spliceosome (C complex) at 4.1 angstrom resolution
Deposited 2017-12-14
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 51
PDB declaration: 55-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
ADP ADENOSINE-5'-DIPHOSPHATE × 2
ZN ZINC ION × 7
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å
|
|
5Z56
cryo-EM structure of a human activated spliceosome (mature Bact) at 5.1 angstrom.
Deposited 2018-01-17
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 55
PDB declaration: 59-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 6
ZN ZINC ION × 13
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9；20 mM HEPES-KOH, 150 mM NaCl, 1.5 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.10 Å
|
|
5Z57
Cryo-EM structure of the human activated spliceosome (late Bact) at 6.5 angstrom
Deposited 2018-01-17
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 53
PDB declaration: 57-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
ALA ALANINE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 6
ZN ZINC ION × 10
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9；20 mM HEPES-KOH, pH 7.9, 150 mM NaCl, 1.5 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.50 Å
|
|
5Z58
Cryo-EM structure of a human activated spliceosome (early Bact) at 4.9 angstrom.
Deposited 2018-01-17
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 42
PDB declaration: 46-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 6
ZN ZINC ION × 4
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9；20 mM HEPES-KOH, pH 7.9, 150 mM NaCl, 1.5 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.90 Å
|
|
6AH0
The Cryo-EM Structure of the Precusor of Human Pre-catalytic Spliceosome (pre-B complex)
Deposited 2018-08-15
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 52
PDB declaration: 57-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.70 Å
|
|
6AHD
The Cryo-EM Structure of Human Pre-catalytic Spliceosome (B complex) at 3.8 angstrom resolution
Deposited 2018-08-17
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 58
PDB declaration: 63-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å
|
|
6FF7
human Bact spliceosome core structure
Deposited 2018-01-03
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 57
PDB declaration: 61-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
ZN ZINC ION × 11
MG MAGNESIUM ION × 5
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ADP ADENOSINE-5'-DIPHOSPHATE × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å
|
|
6ICZ
Cryo-EM structure of a human post-catalytic spliceosome (P complex) at 3.0 angstrom
Deposited 2018-09-07
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 47
PDB declaration: 51-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 9
ZN ZINC ION × 7
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å
|
|
6ID0
Cryo-EM structure of a human intron lariat spliceosome prior to Prp43 loaded (ILS1 complex) at 2.9 angstrom resolution
Deposited 2018-09-07
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 38
PDB declaration: 42-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
ZN ZINC ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å
|
|
6ID1
Cryo-EM structure of a human intron lariat spliceosome after Prp43 loaded (ILS2 complex) at 2.9 angstrom resolution
Deposited 2018-09-07
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 39
PDB declaration: 43-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
ZN ZINC ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.86 Å
|
|
6QDV
Human post-catalytic P complex spliceosome
Deposited 2019-01-03
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 49
PDB declaration: 54-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
MG MAGNESIUM ION × 7
K POTASSIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ZN ZINC ION × 7
IHP INOSITOL HEXAKISPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；3 uL sample was applied to the grid, left for 25s, then blotted for 2.5-3.5s and immediately plunged into liquid ethane.
|
Resolution 3.30 Å
|
|
6QW6
Structure of the human U5.U4/U6 tri-snRNP at 2.9A resolution.
Deposited 2019-03-05
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 36
PDB declaration: 39-meric
|
Chain 5A
25–2335（2311 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
MG MAGNESIUM ION × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ZN ZINC ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Wait 30s, blot for 2s to 3s.
|
Resolution 2.92 Å
|
|
6QX9
Structure of a human fully-assembled precatalytic spliceosome (pre-B complex).
Deposited 2019-03-07
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 65
PDB declaration: 71-meric
|
Chain 5A
25–2335（2311 aa）
|
Not recorded
|
ZN ZINC ION × 6
MG MAGNESIUM ION × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
IHP INOSITOL HEXAKISPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.28 Å
|
|
6S8Q
Human Brr2 Helicase Region in complex with C-tail deleted Jab1
Deposited 2019-07-10
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain J
2064–2320（257 aa）
|
Not recorded
|
SO4 SULFATE ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291.15 K；0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350
|
Resolution 2.39 Å
R-free 0.253
|
|
6S9I
Human Brr2 Helicase Region D534C/N1866C in complex with C-tail deleted Jab1
Deposited 2019-07-13
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain J
2064–2320（257 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291.15 K；0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350
|
Resolution 2.60 Å
R-free 0.278
|
|
6ZYM
Human C Complex Spliceosome - High-resolution CORE
Deposited 2020-08-02
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
|
Assembly 1
Protein–RNA
Heteromer；Protein × 21
PDB declaration: 26-meric
|
Chain A
1–1755（1755 aa）
|
Not recorded
|
MG MAGNESIUM ION × 5
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ZN ZINC ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å
|
|
7A5P
Human C Complex Spliceosome - Medium-resolution PERIPHERY
Deposited 2020-08-21
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 44
PDB declaration: 48-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.00 Å
|
|
7AAV
Human pre-Bact-2 spliceosome core structure
Deposited 2020-09-04
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 13
PDB declaration: heptadecameric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
KGN D-chiro inositol hexakisphosphate × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å
|
|
7ABF
Human pre-Bact-1 spliceosome core structure
Deposited 2020-09-07
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 12
PDB declaration: pentadecameric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å
|
|
7ABG
Human pre-Bact-1 spliceosome
Deposited 2020-09-07
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 54
PDB declaration: 58-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
GTG 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-GUANOSINE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.80 Å
|
|
7ABI
Human pre-Bact-2 spliceosome
Deposited 2020-09-07
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 52
PDB declaration: 56-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
IHP INOSITOL HEXAKISPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.00 Å
|
|
7BDI
Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and ATPgammaS
Deposited 2020-12-21
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain J
2064–2320（257 aa）
|
Not recorded
|
AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 2
MG MAGNESIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291.15 K；0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350
|
Resolution 2.80 Å
R-free 0.297
|
|
7BDJ
Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and mant-ATPgammaS
Deposited 2020-12-21
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain J
2064–2320（257 aa）
|
Not recorded
|
TGB [[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3-[2-(methylamino)phenyl]carbonyloxy-4-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-sulfanyl-phosphinic acid × 2
MG MAGNESIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291.15 K；0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350
|
Resolution 2.59 Å
R-free 0.289
|
|
7BDK
Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and ADP
Deposited 2020-12-21
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain J
2064–2320（257 aa）
|
Not recorded
|
ADP ADENOSINE-5'-DIPHOSPHATE × 2
MG MAGNESIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291.15 K；0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350
|
Resolution 2.52 Å
R-free 0.284
|
|
7BDL
Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and mant-ADP
Deposited 2020-12-21
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain J
2064–2320（257 aa）
|
Not recorded
|
TG8 [(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-2-[[oxidanyl(phosphonooxy)phosphoryl]oxymethyl]oxolan-3-yl] 2-(methylamino)benzoate × 2
MG MAGNESIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291.15 K；0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350
|
Resolution 2.69 Å
R-free 0.292
|
|
7DVQ
Cryo-EM Structure of the Activated Human Minor Spliceosome (minor Bact Complex)
Deposited 2021-01-14
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 45
PDB declaration: 49-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 5
K POTASSIUM ION × 2
G5J 5'-O-[(S)-hydroxy{[(R)-hydroxy{[(S)-hydroxy(methoxy)phosphoryl]oxy}phosphoryl]oxy}phosphoryl]guanosine × 1
ZN ZINC ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.89 Å
|
|
7OS2
Cryo-EM structure of Brr2 in complex with Jab1/MPN and C9ORF78
Deposited 2021-06-07
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain J
2064–2335（272 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.76 Å
|
|
7PJH
Crystal structure of the human spliceosomal maturation factor AAR2 bound to the RNAse H domain of PRPF8
Deposited 2021-08-24
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain B
1758–2016（259 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；0.1 M HEPES pH 7.0, 10% PEG 6000, 5%(v/v) 2-methyl-2,4- pentanediol
|
Resolution 2.35 Å
R-free 0.235
|
|
7PX3
Structure of U5 snRNP assembly and recycling factor TSSC4 in complex with BRR2 and Jab1 domain of PRPF8
Deposited 2021-10-07
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 3
PDB declaration: trimeric
|
Chain J
2064–2320（257 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.05 Å
|
|
7QTT
Structural organization of a late activated human spliceosome (Baqr, core region)
Deposited 2022-01-15
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 33
PDB declaration: 37-meric
|
Chain a
1–2335（2335 aa）
|
Not recorded
|
ZN ZINC ION × 12
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9；All solutions were sterile filtered using a 0.22um vacuum filtration unit.
cryo-EM vitrification conditions
Cryogen ETHANE；Volumes of 4 ul of the concentrated sample were applied to one side of glow-discharged UltrAuFoil 200 2/2 grids (Quantifoil) in a Vitrobot Mark IV (FEI) operating at 4 degrees Celsius and 100% humidity. The grids were blotted for 2s with blotting force 5 and immediately frozen by plunging into liquid ethane.
|
Resolution 3.10 Å
|
|
7W59
The cryo-EM structure of human pre-C*-I complex
Deposited 2021-11-29
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 45
PDB declaration: 50-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 9
ZN ZINC ION × 6
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å
|
|
7W5A
The cryo-EM structure of human pre-C*-II complex
Deposited 2021-11-29
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 49
PDB declaration: 54-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 9
ZN ZINC ION × 7
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å
|
|
7W5B
The cryo-EM structure of human C* complex
Deposited 2021-11-29
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 50
PDB declaration: 55-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 9
ZN ZINC ION × 7
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å
|
|
8BC8
Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and compound 18
Deposited 2022-10-15
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain J
2064–2320（257 aa）
|
Not recorded
|
QB6 3-azanyl-4-oxidanyl-benzenesulfonamide × 4
EDO 1,2-ETHANEDIOL × 16
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291.15 K；0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350
|
Resolution 2.39 Å
R-free 0.245
|
|
8BC9
Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and compound 24
Deposited 2022-10-15
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain J
2064–2320（257 aa）
|
Not recorded
|
A09 N-hydroxybenzenesulfonamide × 1
EDO 1,2-ETHANEDIOL × 11
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291.15 K；0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350
|
Resolution 2.30 Å
R-free 0.249
|
|
8BCA
Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and compound 26
Deposited 2022-10-15
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain J
2064–2320（257 aa）
|
Not recorded
|
Q96 3-azanyl-~{N}-methyl-4-(methylamino)benzenesulfonamide × 2
EDO 1,2-ETHANEDIOL × 9
GOL GLYCEROL × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291.15 K；0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350
|
Resolution 2.80 Å
R-free 0.258
|
|
8BCB
Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and compound 34
Deposited 2022-10-15
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain J
2064–2320（257 aa）
|
Not recorded
|
EDO 1,2-ETHANEDIOL × 16
SAN SULFANILAMIDE × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291.15 K；0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350
|
Resolution 2.38 Å
R-free 0.258
|
|
8BCC
Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and compound 39
Deposited 2022-10-15
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain J
2064–2320（257 aa）
|
Not recorded
|
QAU 3-oxidanylbenzenesulfonamide × 3
EDO 1,2-ETHANEDIOL × 10
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291.15 K；0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350
|
Resolution 2.35 Å
R-free 0.258
|
|
8BCD
Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and compound 50
Deposited 2022-10-15
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain J
2064–2320（257 aa）
|
Not recorded
|
QB0 phenylsulfonylcarbamodithioic acid × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291.15 K；0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350
|
Resolution 3.50 Å
R-free 0.279
|
|
8BCE
Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and compound 76
Deposited 2022-10-15
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain J
2064–2320（257 aa）
|
Not recorded
|
B09 N-methoxybenzenesulfonamide × 1
EDO 1,2-ETHANEDIOL × 12
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291.15 K；0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350
|
Resolution 2.05 Å
R-free 0.267
|
|
8BCF
Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and compound 78
Deposited 2022-10-15
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain J
2064–2320（257 aa）
|
Not recorded
|
EDO 1,2-ETHANEDIOL × 10
Q8Z ~{N}-methoxy-~{N}-methyl-benzenesulfonamide × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；291.15 K；0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350
|
Resolution 2.42 Å
R-free 0.275
|
|
8BCG
Human Brr2 Helicase Region in complex with C-tail deleted Jab1 and compound 86
Deposited 2022-10-15
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain J
2064–2320（257 aa）
|
Not recorded
|
Q9I 4-chloranyl-~{N}-methoxy-~{N}-methyl-benzenesulfonamide × 1
EDO 1,2-ETHANEDIOL × 9
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 8；291.15 K；0.1M Hepes-NaOH, 0.1M MgCl2, 8% PEG 3350
|
Resolution 2.39 Å
R-free 0.269
|
|
8C6J
Human spliceosomal PM5 C* complex
Deposited 2023-01-12
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 58
PDB declaration: 63-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
K POTASSIUM ION × 2
MG MAGNESIUM ION × 7
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ZN ZINC ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å
|
|
8CH6
Structure of a late-stage activated spliceosome (BAqr) arrested with a dominant-negative Aquarius mutant (state B complex).
Deposited 2023-02-07
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 55
PDB declaration: 59-meric
|
Chain a
1–2335（2335 aa）
|
Not recorded
|
ZN ZINC ION × 12
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9；All solutions were sterile-filtered using a 0.22um vacuum filtration unit.
cryo-EM vitrification conditions
Cryogen ETHANE；Volumes of 4 ul of the concentrated sample were applied to one side of glow-discharged UltrAuFoil 200 2/2 grids (Quantifoil) in a Vitrobot Mark IV (FEI), operated at 4 degrees Celsius and 100% humidity. The grids were blotted for 2s with blotting force 5 and immediately frozen by plunging into liquid ethane.
|
Resolution 5.90 Å
|
|
8H6E
Cryo-EM structure of human exon-defined spliceosome in the late pre-B state.
Deposited 2022-10-17
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 56
PDB declaration: 61-meric
|
Chain 5B
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
MG MAGNESIUM ION × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ZN ZINC ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å
|
|
8H6J
Cryo-EM structure of human exon-defined spliceosome in the mature pre-B state.
Deposited 2022-10-18
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 56
PDB declaration: 61-meric
|
Chain 5B
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
MG MAGNESIUM ION × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ZN ZINC ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.25 Å
|
|
8H6K
Cryo-EM structure of human exon-defined spliceosome in the mature B state.
Deposited 2022-10-18
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 58
PDB declaration: 63-meric
|
Chain 5B
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
ZN ZINC ION × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å
|
|
8H6L
Cryo-EM structure of human exon-defined spliceosome in the early B state.
Deposited 2022-10-18
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 54
PDB declaration: 59-meric
|
Chain 5B
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
ZN ZINC ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å
|
|
8I0P
The cryo-EM structure of human pre-Bact complex
Deposited 2023-01-11
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
|
Assembly 1
Protein–RNA
Heteromer；Protein × 47
PDB declaration: 51-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
ZN ZINC ION × 4
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å
|
|
8I0R
The cryo-EM structure of human Bact-I complex
Deposited 2023-01-11
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 51
PDB declaration: 55-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 6
ZN ZINC ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å
|
|
8I0S
The cryo-EM structure of human Bact-II complex
Deposited 2023-01-11
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 46
PDB declaration: 50-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
ZN ZINC ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å
|
|
8I0T
The cryo-EM structure of human Bact-III complex
Deposited 2023-01-11
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 52
PDB declaration: 56-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
ZN ZINC ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å
|
|
8I0U
The cryo-EM structure of human Bact-IV complex
Deposited 2023-01-11
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 49
PDB declaration: 53-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
ZN ZINC ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å
|
|
8I0V
The cryo-EM structure of human post-Bact complex
Deposited 2023-01-11
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 47
PDB declaration: 51-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
ZN ZINC ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å
|
|
8I0W
The cryo-EM structure of human C complex
Deposited 2023-01-11
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
|
Assembly 1
Protein–RNA
Heteromer；Protein × 53
PDB declaration: 58-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 5
ZN ZINC ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å
|
|
8Q7N
cryo-EM structure of the human spliceosomal B complex protomer (tri-snRNP core region)
Deposited 2023-08-16
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 17
PDB declaration: 21-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å
|
|
8Q7Q
Structure of the recycling U5 snRNP bound to chaperones CD2BP2 and TSSC4 (State 2)
Deposited 2023-08-16
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 14
PDB declaration: 15-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
MG MAGNESIUM ION × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å
|
|
8Q7V
Structure of the recycling U5 snRNP bound to chaperones CD2BP2 and TSSC4 (State 1)
Deposited 2023-08-17
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 14
PDB declaration: 15-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
MG MAGNESIUM ION × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å
|
|
8Q7W
Structure of the recycling U5 snRNP bound to chaperone CD2BP2 (State 3)
Deposited 2023-08-17
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 14
PDB declaration: 15-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
MG MAGNESIUM ION × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å
|
|
8Q7X
Structure of the recycling U5 snRNP bound to chaperone CD2BP2 (State 4)
Deposited 2023-08-17
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 14
PDB declaration: 15-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
MG MAGNESIUM ION × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å
|
|
8Q91
Structure of the human 20S U5 snRNP core
Deposited 2023-08-19
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 13
PDB declaration: 14-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å
|
|
8QO9
Cryo-EM structure of a human spliceosomal B complex protomer
Deposited 2023-09-28
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 66
PDB declaration: 71-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.29 Å
|
|
8QOZ
Cryo-EM Structure of Pre-B+5'ss+ATPgammaS Complex (core part)
Deposited 2023-09-29
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 13
PDB declaration: 17-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å
|
|
8QP8
Cryo-EM Structure of Pre-B Complex (core part)
Deposited 2023-09-30
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 12
PDB declaration: 15-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å
|
|
8QP9
Cryo-EM Structure of Pre-B+AMPPNP Complex (core part)
Deposited 2023-09-30
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 13
PDB declaration: 16-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å
|
|
8QPA
Cryo-EM Structure of Pre-B+5'ssLNG Complex (core part)
Deposited 2023-10-01
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 13
PDB declaration: heptadecameric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å
|
|
8QPB
Cryo-EM Structure of Pre-B+ATP Complex (core part)
Deposited 2023-10-01
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 13
PDB declaration: 17-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.70 Å
|
|
8QPE
Cryo-EM Structure of Pre-B-like Complex (core part)
Deposited 2023-10-01
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 15
PDB declaration: 20-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å
|
|
8QPK
Cryo-EM Structure of Pre-B+5'ss Complex (core part)
Deposited 2023-10-02
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 12
PDB declaration: hexadecameric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å
|
|
8QXD
Cryo-EM structure of the cross-exon pre-B complex
Deposited 2023-10-24
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 56
PDB declaration: 61-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.60 Å
|
|
8QZS
Cryo-EM structure of the cross-exon B-like complex
Deposited 2023-10-29
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 61
PDB declaration: 68-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å
|
|
8R08
Cryo-EM structure of the cross-exon pre-B+AMPPNP complex
Deposited 2023-10-31
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 64
PDB declaration: 71-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.10 Å
|
|
8R09
Cryo-EM structure of the cross-exon pre-B+5'ss+ATPgammaS complex
Deposited 2023-10-31
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 54
PDB declaration: 60-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å
|
|
8R0A
Cryo-EM structure of the cross-exon pre-B+5'ss complex
Deposited 2023-10-31
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 56
PDB declaration: 62-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.80 Å
|
|
8R0B
Cryo-EM structure of the cross-exon pre-B+ATP complex
Deposited 2023-10-31
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 54
PDB declaration: 60-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å
|
|
8RC0
Structure of the human 20S U5 snRNP
Deposited 2023-12-05
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 14
PDB declaration: 15-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å
|
|
8RM5
Cryo-EM structure of the cross-exon pre-B+5'ssLNG+ATPyS complex
Deposited 2024-01-05
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 54
PDB declaration: 60-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.90 Å
|
|
8RO2
Integrative Structure of the human intron lariat Spliceosome (ILS'')
Deposited 2024-01-11
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 36
PDB declaration: 40-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ZN ZINC ION × 3
IHP INOSITOL HEXAKISPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å
|
|
8Y6O
Cryo-EM Structure of the human minor pre-B complex (pre-precatalytic spliceosome) U11 and tri-snRNP part
Deposited 2024-02-02
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 41
PDB declaration: 46-meric
|
Chain C
1–2335（2335 aa）
|
Not recorded
|
MG MAGNESIUM ION × 2
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ZN ZINC ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen NITROGEN
|
Resolution 3.38 Å
|
|
9FMD
Integrative model of the human post-catalytic spliceosome (P-complex)
Deposited 2024-06-05
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 58
PDB declaration: 63-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
MG MAGNESIUM ION × 6
K POTASSIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ZN ZINC ION × 6
IHP INOSITOL HEXAKISPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å
|
|
9R3D
Cryo-EM structure of the human pre-Bact-OTS complex (whole map)
Deposited 2025-05-04
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 14
PDB declaration: 17-meric
|
Chain A
1–2335（2335 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.12 Å
|
