|
1B34
CRYSTAL STRUCTURE OF THE D1D2 SUB-COMPLEX FROM THE HUMAN SNRNP CORE DOMAIN
Deposited 1998-12-17
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain A
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.6；pH 5.6
|
Resolution 2.50 Å
R-free 0.286
|
|
3CW1
Crystal Structure of Human Spliceosomal U1 snRNP
Deposited 2008-04-21
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 9
PDB declaration: decameric
|
Chain B
1–119（119 aa）
|
Not recorded
|
ZN ZINC ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；38-40% MPD, 0.3M KCl, 0.1M MES.KOH, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 5.49 Å
|
|
3CW1
Crystal Structure of Human Spliceosomal U1 snRNP
Deposited 2008-04-21
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein–RNA
Heteromer；Protein × 9
PDB declaration: decameric
|
Chain M
1–119（119 aa）
|
Not recorded
|
ZN ZINC ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；38-40% MPD, 0.3M KCl, 0.1M MES.KOH, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 5.49 Å
|
|
3CW1
Crystal Structure of Human Spliceosomal U1 snRNP
Deposited 2008-04-21
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 3
Protein–RNA
Heteromer；Protein × 9
PDB declaration: decameric
|
Chain N
1–119（119 aa）
|
Not recorded
|
ZN ZINC ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；38-40% MPD, 0.3M KCl, 0.1M MES.KOH, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 5.49 Å
|
|
3CW1
Crystal Structure of Human Spliceosomal U1 snRNP
Deposited 2008-04-21
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 4
Protein–RNA
Heteromer；Protein × 9
PDB declaration: decameric
|
Chain O
1–119（119 aa）
|
Not recorded
|
ZN ZINC ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.6；277 K；38-40% MPD, 0.3M KCl, 0.1M MES.KOH, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 5.49 Å
|
|
3JCR
3D structure determination of the human*U4/U6.U5* tri-snRNP complex
Deposited 2016-01-21
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 33
PDB declaration: 36-meric
|
Chain P
1–119（119 aa）
Chain p
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
20 mM HEPES, pH 7.9, 100 mM KCl, 5 mM MgCl2, 0.1 mM EDTA；pH 7.9；20 mM HEPES, pH 7.9, 100 mM KCl, 5 mM MgCl2, 0.1 mM EDTA
cryo-EM vitrification conditions
Cryogen ETHANE；Plunged into liquid ethane (FEI VITROBOT MARK IV).
|
Resolution 7.00 Å
|
|
3PGW
Crystal structure of human U1 snRNP
Deposited 2010-11-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Other combination
Heteromer；Protein × 9
PDB declaration: undecameric
|
Chain X
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.6；277 K；8-10% PEG 4000, 0.1 M Trisodium citrate, 2 mM EDTA, 100 mM NaCl, 1-2% Anapoe X-305, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 4.40 Å
R-free 0.348
|
|
3PGW
Crystal structure of human U1 snRNP
Deposited 2010-11-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Other combination
Heteromer；Protein × 9
PDB declaration: undecameric
|
Chain U
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.6；277 K；8-10% PEG 4000, 0.1 M Trisodium citrate, 2 mM EDTA, 100 mM NaCl, 1-2% Anapoe X-305, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K
|
Resolution 4.40 Å
R-free 0.348
|
|
4PJO
Minimal U1 snRNP
Deposited 2014-05-12
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 9
PDB declaration: Undecameric
|
Chain C
2–85（84 aa）
|
Mutation：M1V
|
ZN ZINC ION × 1
EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1
MG MAGNESIUM ION × 8
K POTASSIUM ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.4；277 K；Minimal U1 snRNP at 4 mg/ml in 300mM KCl, 20mM KHepes, pH 7.5, 5mM MgCl2, 1mM DTT, 0.15 mM polyamine-9 mixed 1:1 with 7% MPD, 180mM KCl, 5mM MgSO4, 50mM NaHepes pH 6.4. Streak seeded.
|
Resolution 3.30 Å
R-free 0.255
|
|
4PJO
Minimal U1 snRNP
Deposited 2014-05-12
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein–RNA
Heteromer；Protein × 9
PDB declaration: Undecameric
|
Chain c
2–85（84 aa）
|
Mutation：M1V
|
ZN ZINC ION × 1
EPE 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID × 1
MG MAGNESIUM ION × 5
K POTASSIUM ION × 2
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.4；277 K；Minimal U1 snRNP at 4 mg/ml in 300mM KCl, 20mM KHepes, pH 7.5, 5mM MgCl2, 1mM DTT, 0.15 mM polyamine-9 mixed 1:1 with 7% MPD, 180mM KCl, 5mM MgSO4, 50mM NaHepes pH 6.4. Streak seeded.
|
Resolution 3.30 Å
R-free 0.255
|
|
4PJO
Minimal U1 snRNP
Deposited 2014-05-12
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 3
Protein–RNA
Heteromer；Protein × 9
PDB declaration: Undecameric
|
Chain Q
2–85（84 aa）
|
Mutation：M1V
|
ZN ZINC ION × 1
MG MAGNESIUM ION × 4
K POTASSIUM ION × 3
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.4；277 K；Minimal U1 snRNP at 4 mg/ml in 300mM KCl, 20mM KHepes, pH 7.5, 5mM MgCl2, 1mM DTT, 0.15 mM polyamine-9 mixed 1:1 with 7% MPD, 180mM KCl, 5mM MgSO4, 50mM NaHepes pH 6.4. Streak seeded.
|
Resolution 3.30 Å
R-free 0.255
|
|
4PJO
Minimal U1 snRNP
Deposited 2014-05-12
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 4
Protein–RNA
Heteromer；Protein × 9
PDB declaration: Undecameric
|
Chain q
2–85（84 aa）
|
Mutation：M1V
|
ZN ZINC ION × 1
MG MAGNESIUM ION × 3
EOH ETHANOL × 1
CL CHLORIDE ION × 1
SO4 SULFATE ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6.4；277 K；Minimal U1 snRNP at 4 mg/ml in 300mM KCl, 20mM KHepes, pH 7.5, 5mM MgCl2, 1mM DTT, 0.15 mM polyamine-9 mixed 1:1 with 7% MPD, 180mM KCl, 5mM MgSO4, 50mM NaHepes pH 6.4. Streak seeded.
|
Resolution 3.30 Å
R-free 0.255
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain AI
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 10
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain BQ
1–119（119 aa）
|
Not recorded
|
SO4 SULFATE ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 11
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain BY
1–119（119 aa）
|
Not recorded
|
SO4 SULFATE ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 12
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain Bg
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 13
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain Bo
1–119（119 aa）
|
Not recorded
|
SO4 SULFATE ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 14
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain Bw
1–119（119 aa）
|
Not recorded
|
SO4 SULFATE ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 15
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain CA
1–119（119 aa）
|
Not recorded
|
SO4 SULFATE ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 16
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain CI
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 17
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain CQ
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 18
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain CY
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 19
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain Cg
1–119（119 aa）
|
Not recorded
|
SO4 SULFATE ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain AA
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 20
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain Co
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 3
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain AQ
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 4
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain AY
1–119（119 aa）
|
Not recorded
|
SO4 SULFATE ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 5
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain Ag
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 6
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain Ao
1–119（119 aa）
|
Not recorded
|
SO4 SULFATE ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 7
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain Aw
1–119（119 aa）
|
Not recorded
|
SO4 SULFATE ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 8
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain BA
1–119（119 aa）
|
Not recorded
|
SO4 SULFATE ION × 1
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4V98
The 8S snRNP Assembly Intermediate
Deposited 2012-05-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 9
Protein heterocomplex
Heteromer；Protein × 8
PDB declaration: octameric
|
Chain BI
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；289 K；24% PEG 4000, 10% Ethanol, 150mM NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
|
Resolution 3.10 Å
R-free 0.256
|
|
4WZJ
Spliceosomal U4 snRNP core domain
Deposited 2014-11-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 7
PDB declaration: octameric
|
Chain C
1–118（118 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å
R-free 0.224
|
|
4WZJ
Spliceosomal U4 snRNP core domain
Deposited 2014-11-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 10
Protein–RNA
Heteromer；Protein × 7
PDB declaration: octameric
|
Chain CCCC
1–118（118 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å
R-free 0.224
|
|
4WZJ
Spliceosomal U4 snRNP core domain
Deposited 2014-11-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 11
Protein–RNA
Heteromer；Protein × 7
PDB declaration: octameric
|
Chain JJJJ
1–118（118 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å
R-free 0.224
|
|
4WZJ
Spliceosomal U4 snRNP core domain
Deposited 2014-11-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 12
Protein–RNA
Heteromer；Protein × 7
PDB declaration: octameric
|
Chain QQQQ
1–118（118 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å
R-free 0.224
|
|
4WZJ
Spliceosomal U4 snRNP core domain
Deposited 2014-11-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 2
Protein–RNA
Heteromer；Protein × 7
PDB declaration: octameric
|
Chain J
1–118（118 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å
R-free 0.224
|
|
4WZJ
Spliceosomal U4 snRNP core domain
Deposited 2014-11-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 3
Protein–RNA
Heteromer；Protein × 7
PDB declaration: octameric
|
Chain Q
1–118（118 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å
R-free 0.224
|
|
4WZJ
Spliceosomal U4 snRNP core domain
Deposited 2014-11-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 4
Protein–RNA
Heteromer；Protein × 7
PDB declaration: octameric
|
Chain CC
1–118（118 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å
R-free 0.224
|
|
4WZJ
Spliceosomal U4 snRNP core domain
Deposited 2014-11-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 5
Protein–RNA
Heteromer；Protein × 7
PDB declaration: octameric
|
Chain JJ
1–118（118 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å
R-free 0.224
|
|
4WZJ
Spliceosomal U4 snRNP core domain
Deposited 2014-11-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 6
Protein–RNA
Heteromer；Protein × 7
PDB declaration: octameric
|
Chain QQ
1–118（118 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å
R-free 0.224
|
|
4WZJ
Spliceosomal U4 snRNP core domain
Deposited 2014-11-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 7
Protein–RNA
Heteromer；Protein × 7
PDB declaration: octameric
|
Chain CCC
1–118（118 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å
R-free 0.224
|
|
4WZJ
Spliceosomal U4 snRNP core domain
Deposited 2014-11-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 8
Protein–RNA
Heteromer；Protein × 7
PDB declaration: octameric
|
Chain JJJ
1–118（118 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å
R-free 0.224
|
|
4WZJ
Spliceosomal U4 snRNP core domain
Deposited 2014-11-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 9
Protein–RNA
Heteromer；Protein × 7
PDB declaration: octameric
|
Chain QQQ
1–118（118 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；4-10% PEG 550 MME,
0.1M KSCN or (NH4)2SO4,
0.1 M TRIS PH 8.0-8.5
10 MM MGCL2,
2 MM CYCLEN.
|
Resolution 3.60 Å
R-free 0.224
|
|
5MQF
Cryo-EM structure of a human spliceosome activated for step 2 of splicing (C* complex)
Deposited 2016-12-20
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 41
PDB declaration: 41-meric
|
Chain g
1–119（119 aa）
Chain n
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 6.4
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.90 Å
|
|
5O9Z
Cryo-EM structure of a pre-catalytic human spliceosome primed for activation (B complex)
Deposited 2017-06-20
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 52
PDB declaration: 57-meric
|
Chain Z
1–119（119 aa）
Chain g
1–119（119 aa）
Chain n
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å
|
|
5XJC
Cryo-EM structure of the human spliceosome just prior to exon ligation at 3.6 angstrom
Deposited 2017-04-30
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 46
PDB declaration: 50-meric
|
Chain c
1–119（119 aa）
Chain j
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 11
ADP ADENOSINE-5'-DIPHOSPHATE × 2
ZN ZINC ION × 7
ATP ADENOSINE-5'-TRIPHOSPHATE × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å
|
|
5XJL
Crystal Structure of the Gemin2-binding domain of SMN, Gemin2 in Complex with SmD1/D2/F/E/G from Human
Deposited 2017-05-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 7
PDB declaration: heptameric
|
Chain A
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.8；293 K；1%PEG8000, 100mM Tris-HCl
|
Resolution 2.50 Å
R-free 0.296
|
|
5XJQ
Crystal Structure of the Gemin2-binding domain of SMN, Gemin2 in Complex with SmD1(1-82)/D2/F/E/G from Human
Deposited 2017-05-04
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 7
PDB declaration: heptameric
|
Chain A
1–82（82 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；6% PEG8000, 100mM Tris.HCl
|
Resolution 3.28 Å
R-free 0.262
|
|
5XJR
Crystal Structure of the Gemin2-binding domain of SMN, Gemin2dN39 in Complex with SmD1(1-82)/D2/F/E/G from Human
Deposited 2017-05-04
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 7
PDB declaration: heptameric
|
Chain A
1–82（82 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；1% PEG8000, 100mM Tris.HCl
|
Resolution 3.12 Å
R-free 0.242
|
|
5XJS
Crystal Structure of the Gemin2-binding domain of SMN, Gemin2dN39 in Complex with SmD1(1-82)/D2/F/E from Human
Deposited 2017-05-04
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 6
PDB declaration: hexameric
|
Chain A
1–82（82 aa）
Fragment：UNP residues 1-82
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；4% PEG8000, 100mM Tris.HCl
|
Resolution 3.38 Å
R-free 0.248
|
|
5XJT
Crystal Structure of the Gemin2-binding domain of SMN, Gemin2 in Complex with SmD1(1-82)/D2.R61A/F/E/G from Human
Deposited 2017-05-04
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 7
PDB declaration: heptameric
|
Chain A
1–82（82 aa）
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；1% PEG8000, 100mM Tris.HCl
|
Resolution 2.92 Å
R-free 0.281
|
|
5XJU
Crystal Structure of the Gemin2-binding domain of SMN, Gemin2dN39 in Complex with SmD1(1-82)/D2.R61A/F/E/G from Human
Deposited 2017-05-04
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental method
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 7
PDB declaration: heptameric
|
Chain A
1–82（82 aa）
Fragment：UNP residues 1-82
|
Not recorded
|
No recorded non-water small molecule
|
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；1% PEG8000, 100mM Tris.HCl
|
Resolution 2.58 Å
R-free 0.258
|
|
5YZG
The Cryo-EM Structure of Human Catalytic Step I Spliceosome (C complex) at 4.1 angstrom resolution
Deposited 2017-12-14
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 51
PDB declaration: 55-meric
|
Chain c
1–119（119 aa）
Chain j
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
ADP ADENOSINE-5'-DIPHOSPHATE × 2
ZN ZINC ION × 7
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å
|
|
5Z56
cryo-EM structure of a human activated spliceosome (mature Bact) at 5.1 angstrom.
Deposited 2018-01-17
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 55
PDB declaration: 59-meric
|
Chain c
1–119（119 aa）
Chain j
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 6
ZN ZINC ION × 13
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9；20 mM HEPES-KOH, 150 mM NaCl, 1.5 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.10 Å
|
|
5Z57
Cryo-EM structure of the human activated spliceosome (late Bact) at 6.5 angstrom
Deposited 2018-01-17
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 53
PDB declaration: 57-meric
|
Chain c
1–119（119 aa）
Chain j
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
ALA ALANINE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 6
ZN ZINC ION × 10
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9；20 mM HEPES-KOH, pH 7.9, 150 mM NaCl, 1.5 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.50 Å
|
|
5Z58
Cryo-EM structure of a human activated spliceosome (early Bact) at 4.9 angstrom.
Deposited 2018-01-17
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 42
PDB declaration: 46-meric
|
Chain c
1–119（119 aa）
Chain j
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 6
ZN ZINC ION × 4
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9；20 mM HEPES-KOH, pH 7.9, 150 mM NaCl, 1.5 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.90 Å
|
|
6AH0
The Cryo-EM Structure of the Precusor of Human Pre-catalytic Spliceosome (pre-B complex)
Deposited 2018-08-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 52
PDB declaration: 57-meric
|
Chain V
1–119（119 aa）
Chain g
1–119（119 aa）
Chain j
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.70 Å
|
|
6AHD
The Cryo-EM Structure of Human Pre-catalytic Spliceosome (B complex) at 3.8 angstrom resolution
Deposited 2018-08-17
|
Different construct
Different mutation/modification
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 58
PDB declaration: 63-meric
|
Chain V
1–119（119 aa）
Chain b
1–119（119 aa）
Chain j
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å
|
|
6FF7
human Bact spliceosome core structure
Deposited 2018-01-03
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 57
PDB declaration: 61-meric
|
Chain g
1–119（119 aa）
Chain n
1–119（119 aa）
|
Not recorded
|
ZN ZINC ION × 11
MG MAGNESIUM ION × 5
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ADP ADENOSINE-5'-DIPHOSPHATE × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.6
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.50 Å
|
|
6ICZ
Cryo-EM structure of a human post-catalytic spliceosome (P complex) at 3.0 angstrom
Deposited 2018-09-07
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 47
PDB declaration: 51-meric
|
Chain c
1–119（119 aa）
Chain j
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 9
ZN ZINC ION × 7
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å
|
|
6ID0
Cryo-EM structure of a human intron lariat spliceosome prior to Prp43 loaded (ILS1 complex) at 2.9 angstrom resolution
Deposited 2018-09-07
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 38
PDB declaration: 42-meric
|
Chain c
1–119（119 aa）
Chain j
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
ZN ZINC ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.90 Å
|
|
6ID1
Cryo-EM structure of a human intron lariat spliceosome after Prp43 loaded (ILS2 complex) at 2.9 angstrom resolution
Deposited 2018-09-07
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 39
PDB declaration: 43-meric
|
Chain c
1–119（119 aa）
Chain j
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
ZN ZINC ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.86 Å
|
|
6QDV
Human post-catalytic P complex spliceosome
Deposited 2019-01-03
|
Different construct
Different oligomeric state
Different ligand/ion
Different experimental conditions
|
Assembly 1
Protein–RNA
Heteromer；Protein × 49
PDB declaration: 54-meric
|
Chain h
1–80（80 aa）
Chain l
1–80（80 aa）
|
Not recorded
|
MG MAGNESIUM ION × 7
K POTASSIUM ION × 1
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ZN ZINC ION × 7
IHP INOSITOL HEXAKISPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；3 uL sample was applied to the grid, left for 25s, then blotted for 2.5-3.5s and immediately plunged into liquid ethane.
|
Resolution 3.30 Å
|
|
6QW6
Structure of the human U5.U4/U6 tri-snRNP at 2.9A resolution.
Deposited 2019-03-05
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 36
PDB declaration: 39-meric
|
Chain 41
1–119（119 aa）
Chain 51
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
MG MAGNESIUM ION × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ZN ZINC ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE；Wait 30s, blot for 2s to 3s.
|
Resolution 2.92 Å
|
|
6QX9
Structure of a human fully-assembled precatalytic spliceosome (pre-B complex).
Deposited 2019-03-07
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 65
PDB declaration: 71-meric
|
Chain 11
1–119（119 aa）
Chain 21
1–119（119 aa）
Chain 41
1–119（119 aa）
Chain 51
1–119（119 aa）
|
Not recorded
|
ZN ZINC ION × 6
MG MAGNESIUM ION × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
IHP INOSITOL HEXAKISPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.28 Å
|
|
6Y53
human 17S U2 snRNP low resolution part
Deposited 2020-02-24
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 18
PDB declaration: nonadecameric
|
Chain n
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.10 Å
|
|
6Y5Q
human 17S U2 snRNP
Deposited 2020-02-25
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 21
PDB declaration: 22-meric
|
Chain n
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.10 Å
|
|
7A5P
Human C Complex Spliceosome - Medium-resolution PERIPHERY
Deposited 2020-08-21
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 44
PDB declaration: 48-meric
|
Chain c
1–119（119 aa）
Chain n
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.00 Å
|
|
7ABG
Human pre-Bact-1 spliceosome
Deposited 2020-09-07
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 54
PDB declaration: 58-meric
|
Chain g
1–119（119 aa）
Chain n
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
GTG 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-GUANOSINE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.80 Å
|
|
7ABI
Human pre-Bact-2 spliceosome
Deposited 2020-09-07
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 52
PDB declaration: 56-meric
|
Chain g
1–119（119 aa）
Chain n
1–119（119 aa）
|
Not recorded
|
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
IHP INOSITOL HEXAKISPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.00 Å
|
|
7B0Y
Structure of a transcribing RNA polymerase II-U1 snRNP complex
Deposited 2020-11-23
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Other combination
Heteromer；Protein × 21
PDB declaration: 25-meric
|
Chain k
1–119（119 aa）
|
Not recorded
|
ZN ZINC ION × 8
MG MAGNESIUM ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å
|
|
7DVQ
Cryo-EM Structure of the Activated Human Minor Spliceosome (minor Bact Complex)
Deposited 2021-01-14
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 45
PDB declaration: 49-meric
|
Chain c
1–119（119 aa）
Chain j
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 5
K POTASSIUM ION × 2
G5J 5'-O-[(S)-hydroxy{[(R)-hydroxy{[(S)-hydroxy(methoxy)phosphoryl]oxy}phosphoryl]oxy}phosphoryl]guanosine × 1
ZN ZINC ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.89 Å
|
|
7EVO
The cryo-EM structure of the human 17S U2 snRNP
Deposited 2021-05-21
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 20
PDB declaration: 21-meric
|
Chain g
1–119（119 aa）
|
Not recorded
|
9B0 [(2~{S},3~{S},4~{E},6~{S},7~{R},10~{R})-3,7-dimethyl-2-[(2~{E},4~{E},6~{R})-6-methyl-6-oxidanyl-7-[(2~{R},3~{R})-3-[(2~{R},3~{S})-3-oxidanylpentan-2-yl]oxiran-2-yl]hepta-2,4-dien-2-yl]-7,10-bis(oxidanyl)-12-oxidanylidene-1-oxacyclododec-4-en-6-yl] 4-cycloheptylpiperazine-1-carboxylate × 1
ZN ZINC ION × 4
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.50 Å
|
|
7QTT
Structural organization of a late activated human spliceosome (Baqr, core region)
Deposited 2022-01-15
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 33
PDB declaration: 37-meric
|
Chain k
1–119（119 aa）
|
Not recorded
|
ZN ZINC ION × 12
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9；All solutions were sterile filtered using a 0.22um vacuum filtration unit.
cryo-EM vitrification conditions
Cryogen ETHANE；Volumes of 4 ul of the concentrated sample were applied to one side of glow-discharged UltrAuFoil 200 2/2 grids (Quantifoil) in a Vitrobot Mark IV (FEI) operating at 4 degrees Celsius and 100% humidity. The grids were blotted for 2s with blotting force 5 and immediately frozen by plunging into liquid ethane.
|
Resolution 3.10 Å
|
|
7VPX
The cryo-EM structure of the human pre-A complex
Deposited 2021-10-18
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 31
PDB declaration: 35-meric
|
Chain g
1–119（119 aa）
Chain h
1–119（119 aa）
|
Not recorded
|
ZN ZINC ION × 6
SJT spliceostatin A (form II) × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å
|
|
7W59
The cryo-EM structure of human pre-C*-I complex
Deposited 2021-11-29
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 45
PDB declaration: 50-meric
|
Chain c
1–119（119 aa）
Chain j
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 9
ZN ZINC ION × 6
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å
|
|
7W5A
The cryo-EM structure of human pre-C*-II complex
Deposited 2021-11-29
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 49
PDB declaration: 54-meric
|
Chain c
1–119（119 aa）
Chain j
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 9
ZN ZINC ION × 7
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.60 Å
|
|
7W5B
The cryo-EM structure of human C* complex
Deposited 2021-11-29
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 50
PDB declaration: 55-meric
|
Chain c
1–119（119 aa）
Chain j
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 9
ZN ZINC ION × 7
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å
|
|
8C6J
Human spliceosomal PM5 C* complex
Deposited 2023-01-12
|
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 58
PDB declaration: 63-meric
|
Chain c
1–119（119 aa）
Chain l
1–119（119 aa）
|
Not recorded
|
K POTASSIUM ION × 2
MG MAGNESIUM ION × 7
ATP ADENOSINE-5'-TRIPHOSPHATE × 1
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ZN ZINC ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.80 Å
|
|
8CH6
Structure of a late-stage activated spliceosome (BAqr) arrested with a dominant-negative Aquarius mutant (state B complex).
Deposited 2023-02-07
|
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 55
PDB declaration: 59-meric
|
Chain 5
1–119（119 aa）
Chain k
1–119（119 aa）
|
Not recorded
|
ZN ZINC ION × 12
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9；All solutions were sterile-filtered using a 0.22um vacuum filtration unit.
cryo-EM vitrification conditions
Cryogen ETHANE；Volumes of 4 ul of the concentrated sample were applied to one side of glow-discharged UltrAuFoil 200 2/2 grids (Quantifoil) in a Vitrobot Mark IV (FEI), operated at 4 degrees Celsius and 100% humidity. The grids were blotted for 2s with blotting force 5 and immediately frozen by plunging into liquid ethane.
|
Resolution 5.90 Å
|
|
8H6E
Cryo-EM structure of human exon-defined spliceosome in the late pre-B state.
Deposited 2022-10-17
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 56
PDB declaration: 61-meric
|
Chain 2b
1–119（119 aa）
Chain 4b
1–119（119 aa）
Chain 5b
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
MG MAGNESIUM ION × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ZN ZINC ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å
|
|
8H6J
Cryo-EM structure of human exon-defined spliceosome in the mature pre-B state.
Deposited 2022-10-18
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 56
PDB declaration: 61-meric
|
Chain 2b
1–119（119 aa）
Chain 4b
1–119（119 aa）
Chain 5b
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
MG MAGNESIUM ION × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ZN ZINC ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.25 Å
|
|
8H6K
Cryo-EM structure of human exon-defined spliceosome in the mature B state.
Deposited 2022-10-18
|
Different construct
Different mutation/modification
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 58
PDB declaration: 63-meric
|
Chain 2b
1–119（119 aa）
Chain 4b
1–119（119 aa）
Chain 5b
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
ZN ZINC ION × 2
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å
|
|
8H6L
Cryo-EM structure of human exon-defined spliceosome in the early B state.
Deposited 2022-10-18
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 54
PDB declaration: 59-meric
|
Chain 2b
1–119（119 aa）
Chain 4b
1–119（119 aa）
Chain 5b
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
ZN ZINC ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.60 Å
|
|
8HK1
The cryo-EM structure of human pre-17S U2 snRNP
Deposited 2022-11-24
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 20
PDB declaration: 21-meric
|
Chain g
1–119（119 aa）
|
Not recorded
|
9B0 [(2~{S},3~{S},4~{E},6~{S},7~{R},10~{R})-3,7-dimethyl-2-[(2~{E},4~{E},6~{R})-6-methyl-6-oxidanyl-7-[(2~{R},3~{R})-3-[(2~{R},3~{S})-3-oxidanylpentan-2-yl]oxiran-2-yl]hepta-2,4-dien-2-yl]-7,10-bis(oxidanyl)-12-oxidanylidene-1-oxacyclododec-4-en-6-yl] 4-cycloheptylpiperazine-1-carboxylate × 1
ZN ZINC ION × 4
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 2.70 Å
|
|
8I0P
The cryo-EM structure of human pre-Bact complex
Deposited 2023-01-11
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 47
PDB declaration: 51-meric
|
Chain b
1–119（119 aa）
Chain n
1–119（119 aa）
|
Not recorded
|
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 1
ZN ZINC ION × 4
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å
|
|
8I0R
The cryo-EM structure of human Bact-I complex
Deposited 2023-01-11
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 51
PDB declaration: 55-meric
|
Chain b
1–119（119 aa）
Chain n
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 6
ZN ZINC ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å
|
|
8I0S
The cryo-EM structure of human Bact-II complex
Deposited 2023-01-11
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 46
PDB declaration: 50-meric
|
Chain b
1–119（119 aa）
Chain n
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
ZN ZINC ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.20 Å
|
|
8I0T
The cryo-EM structure of human Bact-III complex
Deposited 2023-01-11
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 52
PDB declaration: 56-meric
|
Chain b
1–119（119 aa）
Chain n
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
ZN ZINC ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å
|
|
8I0U
The cryo-EM structure of human Bact-IV complex
Deposited 2023-01-11
|
Different oligomeric state
Different ligand/ion
|
Assembly 1
Protein–RNA
Heteromer；Protein × 49
PDB declaration: 53-meric
|
Chain b
1–119（119 aa）
Chain n
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
ZN ZINC ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.30 Å
|
|
8I0V
The cryo-EM structure of human post-Bact complex
Deposited 2023-01-11
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 47
PDB declaration: 51-meric
|
Chain b
1–119（119 aa）
Chain n
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 7
ZN ZINC ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å
|
|
8I0W
The cryo-EM structure of human C complex
Deposited 2023-01-11
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 53
PDB declaration: 58-meric
|
Chain b
1–119（119 aa）
Chain j
1–119（119 aa）
|
Not recorded
|
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
MG MAGNESIUM ION × 5
ZN ZINC ION × 7
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å
|
|
8Q7Q
Structure of the recycling U5 snRNP bound to chaperones CD2BP2 and TSSC4 (State 2)
Deposited 2023-08-16
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 14
PDB declaration: 15-meric
|
Chain a
1–119（119 aa）
|
Not recorded
|
MG MAGNESIUM ION × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å
|
|
8Q7V
Structure of the recycling U5 snRNP bound to chaperones CD2BP2 and TSSC4 (State 1)
Deposited 2023-08-17
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 14
PDB declaration: 15-meric
|
Chain a
1–119（119 aa）
|
Not recorded
|
MG MAGNESIUM ION × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.80 Å
|
|
8Q7W
Structure of the recycling U5 snRNP bound to chaperone CD2BP2 (State 3)
Deposited 2023-08-17
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 14
PDB declaration: 15-meric
|
Chain a
1–119（119 aa）
|
Not recorded
|
MG MAGNESIUM ION × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.90 Å
|
|
8Q7X
Structure of the recycling U5 snRNP bound to chaperone CD2BP2 (State 4)
Deposited 2023-08-17
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 14
PDB declaration: 15-meric
|
Chain a
1–119（119 aa）
|
Not recorded
|
MG MAGNESIUM ION × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.60 Å
|
|
8Q91
Structure of the human 20S U5 snRNP core
Deposited 2023-08-19
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 13
PDB declaration: 14-meric
|
Chain i
1–119（119 aa）
|
Not recorded
|
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.10 Å
|
|
8QO9
Cryo-EM structure of a human spliceosomal B complex protomer
Deposited 2023-09-28
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 66
PDB declaration: 71-meric
|
Chain 21
1–119（119 aa）
Chain 41
1–119（119 aa）
Chain 51
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.29 Å
|
|
8QXD
Cryo-EM structure of the cross-exon pre-B complex
Deposited 2023-10-24
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 56
PDB declaration: 61-meric
|
Chain 21
1–119（119 aa）
Chain 41
1–119（119 aa）
Chain 51
1–82（82 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 9.60 Å
|
|
8QZS
Cryo-EM structure of the cross-exon B-like complex
Deposited 2023-10-29
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 61
PDB declaration: 68-meric
|
Chain 21
1–119（119 aa）
Chain 41
1–119（119 aa）
Chain 51
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.10 Å
|
|
8R08
Cryo-EM structure of the cross-exon pre-B+AMPPNP complex
Deposited 2023-10-31
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 64
PDB declaration: 71-meric
|
Chain 11
1–119（119 aa）
Chain 21
1–119（119 aa）
Chain 41
1–119（119 aa）
Chain 51
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.10 Å
|
|
8R09
Cryo-EM structure of the cross-exon pre-B+5'ss+ATPgammaS complex
Deposited 2023-10-31
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 54
PDB declaration: 60-meric
|
Chain 21
1–119（119 aa）
Chain 41
1–119（119 aa）
Chain 51
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.30 Å
|
|
8R0A
Cryo-EM structure of the cross-exon pre-B+5'ss complex
Deposited 2023-10-31
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 56
PDB declaration: 62-meric
|
Chain 21
1–119（119 aa）
Chain 41
1–119（119 aa）
Chain 51
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.80 Å
|
|
8R0B
Cryo-EM structure of the cross-exon pre-B+ATP complex
Deposited 2023-10-31
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 54
PDB declaration: 60-meric
|
Chain 21
1–119（119 aa）
Chain 41
1–119（119 aa）
Chain 51
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 4.40 Å
|
|
8R7N
Overall structure of the U11 snRNP
Deposited 2023-11-26
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 12
PDB declaration: 13-meric
|
Chain h
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9；20 mM HEPES-KOH, pH 7.9
200 mM KCl
2 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.40 Å
|
|
8RC0
Structure of the human 20S U5 snRNP
Deposited 2023-12-05
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 14
PDB declaration: 15-meric
|
Chain i
1–119（119 aa）
|
Not recorded
|
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.8
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å
|
|
8RM5
Cryo-EM structure of the cross-exon pre-B+5'ssLNG+ATPyS complex
Deposited 2024-01-05
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 54
PDB declaration: 60-meric
|
Chain 21
1–119（119 aa）
Chain 41
1–119（119 aa）
Chain 51
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 6.90 Å
|
|
8RO2
Integrative Structure of the human intron lariat Spliceosome (ILS'')
Deposited 2024-01-11
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 36
PDB declaration: 40-meric
|
Chain c
1–119（119 aa）
|
Not recorded
|
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ZN ZINC ION × 3
IHP INOSITOL HEXAKISPHOSPHATE × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å
|
|
8Y6O
Cryo-EM Structure of the human minor pre-B complex (pre-precatalytic spliceosome) U11 and tri-snRNP part
Deposited 2024-02-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 41
PDB declaration: 46-meric
|
Chain b
1–119（119 aa）
Chain i
1–119（119 aa）
Chain p
1–119（119 aa）
|
Not recorded
|
MG MAGNESIUM ION × 2
IHP INOSITOL HEXAKISPHOSPHATE × 1
GTP GUANOSINE-5'-TRIPHOSPHATE × 1
ZN ZINC ION × 6
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen NITROGEN
|
Resolution 3.38 Å
|
|
8Y7E
Cryo-EM Structure of the human minor pre-B complex (pre-precatalytic spliceosome) U12 snRNP part
Deposited 2024-02-04
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 15
PDB declaration: heptadecameric
|
Chain j
1–119（119 aa）
|
Not recorded
|
ZN ZINC ION × 4
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen NITROGEN
|
Resolution 4.66 Å
|
|
9E3B
Cryo-EM structure of PRMT5/WDR77 in complex with 6S complex
Deposited 2024-10-23
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 16
PDB declaration: hexadecameric
|
Chain M
1–119（119 aa）
Chain N
1–119（119 aa）
Chain O
1–119（119 aa）
Chain P
1–119（119 aa）
|
Not recorded
|
SFG SINEFUNGIN × 4
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；30 mM HEPES pH 7.4, 150 mM NaCl, 3 mM TCEP, 3.33 mM sinefungin
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.06 Å
|
|
9E3C
Cryo-EM structure of PRMT5/WDR77 in complex with 6S complex (GRG local refine)
Deposited 2024-10-23
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein heterocomplex
Heteromer；Protein × 2
PDB declaration: dimeric
|
Chain M
1–119（119 aa）
|
Not recorded
|
SFG SINEFUNGIN × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.4；30 mM HEPES pH 7.4, 150 mM NaCl, 3 mM TCEP, 3.33 mM sinefungin
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.19 Å
|
|
9GBW
Overall structure of the substrate-bound U11 snRNP
Deposited 2024-07-31
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 12
PDB declaration: 14-meric
|
Chain h
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9；20 mM HEPES-KOH, pH 7.9
200 mM KCl
2 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å
|
|
9GC0
3'-lobe of the substrate-bound U11 snRNP
Deposited 2024-07-31
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 10
PDB declaration: 11-meric
|
Chain h
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9；20 mM HEPES-KOH, pH 7.9
200 mM KCl
2 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.20 Å
|
|
9GCL
Structure of the U11 snRNP C-lobe
Deposited 2024-08-02
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 9
PDB declaration: decameric
|
Chain h
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9；20 mM HEPES-KOH, pH 7.9
200 mM KCl
2 mM MgCl2
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.00 Å
|
|
9QEQ
Structure of the transcribing Pol II-DSIF-SPT6-U1 snRNP complex
Deposited 2025-03-10
|
Different construct
Different mutation/modification
Different oligomeric state
Different ligand/ion
Different experimental conditions
Different structure-quality metrics
|
Assembly 1
Other combination
Heteromer；Protein × 24
PDB declaration: 28-meric
|
Chain k
1–119（119 aa）
|
Not recorded
|
ZN ZINC ION × 8
MG MAGNESIUM ION × 1
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.5；20 mM Hepes, 100 mM NaCl, 3 mM MgCl2, 0.5 mM TCEP
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 3.50 Å
|
|
9R8V
Cryo-EM structure of the human pre-Bact-OTS complex (whole map)
Deposited 2025-05-17
|
Different oligomeric state
Different ligand/ion
Different structure-quality metrics
|
Assembly 1
Protein–RNA
Heteromer；Protein × 59
PDB declaration: 63-meric
|
Chain 21
1–119（119 aa）
Chain 51
1–119（119 aa）
|
Not recorded
|
No recorded non-water small molecule
|
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.50 Å
|
