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1b2s

STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE

Method: X-RAY DIFFRACTION
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1. Protein Identity and Related Structures Protein Identity & Related Structures

PROTEIN (BARNASE)

Bacillus amyloliquefaciens

UniProt P00648

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 2 PROTEIN (BARSTAR) × 1 （P11540） water × 2 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 2 PROTEIN (BARSTAR) × 1 （P11540） SULFATE ION × 2 water × 2 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 2 PROTEIN (BARSTAR) × 1 （P11540） water × 2 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1a2p 1a2p 1a2p 1b20 1b20 1b20 1b21 1b21 1b21 1b2x 1b2x 1b2x

89 assemblies; showing the first 12

Homodimer
2f56 3kch 3kch 3kch

4 assemblies

Homotetramer

None

Other homooligomers
1yvs 1yvs

2 assemblies

Heteromeric or nucleic-acid complex
1b27 1b27 1b27 1b2s 1b2s 1b2s 1b2u 1b2u 1b2u 1b3s 1b3s 1b3s

43 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name RNBR_BACAM
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–110； UniProt 48–157 Author chain B； PDBConstruct 1–110； UniProt 48–157 Author chain C； PDBConstruct 1–110； UniProt 48–157

PROTEIN (BARSTAR)

Bacillus amyloliquefaciens

UniProt P11540

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 2 PROTEIN (BARNASE) × 1 （P00648） water × 2 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 2 PROTEIN (BARNASE) × 1 （P00648） SULFATE ION × 2 water × 2 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 2 PROTEIN (BARNASE) × 1 （P00648） water × 2 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1ab7 1bta 1btb 1l1k 2hxx 2hxx 2za4 2za4

8 assemblies

Homodimer
1a19

1 assemblies

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1ay7 1b27 1b27 1b27 1b2s 1b2s 1b2s 1b2u 1b2u 1b2u 1b3s 1b3s

42 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name BARS_BACAM
Isoform —
PDB entities 2
Chains and sequence ranges Author chain D； PDBConstruct 2–90； UniProt 1–89 Author chain E； PDBConstruct 2–90； UniProt 1–89 Author chain F； PDBConstruct 2–90； UniProt 1–89

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id1b2s
Deposition date deposition_date1998-11-30
Structure title titleSTRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE
Keywords keywordsRNASE-INHIBITOR COMPLEX, INTERFACIAL DOUBLE MUTANT, HYDROLASE-HYDROLASE INHIBITOR COMPLEX; HYDROLASE/HYDROLASE INHIBITOR
Experimental Method methodX-RAY DIFFRACTION
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1b2s__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1b2s__assembly_1__model_1 | I(q)

10-2 10-1 105 106 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

1b2s__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）18.38 Å
Rg (electron density)17.49 Å
Total Rg18.51 Å
Atom count1598
Residues200
Excluded volume28309 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 1b2s__assembly_1__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 1b2s__assembly_2__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 1b2s__assembly_3__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (4)

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6. Fold Classification (SCOP + CATH) 12 domains

SCOP 2.08 (6 domains)

Domain ID domain_idd1b2sa_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.1 — Microbial ribonucleases
Superfamily Superfamily superfamilyd.1.1 — Microbial ribonucleases
Family Family familyd.1.1.2 — Bacterial ribonucleases
Domain ID domain_idd1b2sb_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.1 — Microbial ribonucleases
Superfamily Superfamily superfamilyd.1.1 — Microbial ribonucleases
Family Family familyd.1.1.2 — Bacterial ribonucleases
Domain ID domain_idd1b2sc_
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.1 — Microbial ribonucleases
Superfamily Superfamily superfamilyd.1.1 — Microbial ribonucleases
Family Family familyd.1.1.2 — Bacterial ribonucleases
Domain ID domain_idd1b2sd_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.9 — Barstar-like
Superfamily Superfamily superfamilyc.9.1 — Barstar-related
Family Family familyc.9.1.1 — Barstar-related
Domain ID domain_idd1b2se_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.9 — Barstar-like
Superfamily Superfamily superfamilyc.9.1 — Barstar-related
Family Family familyc.9.1.1 — Barstar-related
Domain ID domain_idd1b2sf_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.9 — Barstar-like
Superfamily Superfamily superfamilyc.9.1 — Barstar-related
Family Family familyc.9.1.1 — Barstar-related

CATH v4.4 (6 domains)

Domain ID domain_id1b2sA00
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology450 — Nuclear Transport Factor 2; Chain: A,
Homologous superfamily homologous superfamily30 — Microbial ribonucleases
Domain ID domain_id1b2sB00
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology450 — Nuclear Transport Factor 2; Chain: A,
Homologous superfamily homologous superfamily30 — Microbial ribonucleases
Domain ID domain_id1b2sC00
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology450 — Nuclear Transport Factor 2; Chain: A,
Homologous superfamily homologous superfamily30 — Microbial ribonucleases
Domain ID domain_id1b2sD00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology370 — Barnase; Chain D
Homologous superfamily homologous superfamily10 — Barstar-like
Domain ID domain_id1b2sE00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology370 — Barnase; Chain D
Homologous superfamily homologous superfamily10 — Barstar-like
Domain ID domain_id1b2sF00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology370 — Barnase; Chain D
Homologous superfamily homologous superfamily10 — Barstar-like
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7. Citations (6)