← Back to search

1ay7

RIBONUCLEASE SA COMPLEX WITH BARSTAR

Method: X-RAY DIFFRACTION D_max: 60.0 Å Quality: GOOD

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	1ay7
deposition_date	1997-11-14
title	RIBONUCLEASE SA COMPLEX WITH BARSTAR
keywords	RIBONUCLEASE, INHIBITOR, STREPTOMYCES AUREOFACIENS, COMPLEX (ENZYME-INHIBITOR), COMPLEX (ENZYME-INHIBITOR) complex; COMPLEX (ENZYME/INHIBITOR)
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	18.32 Å
rg_electron	17.38 Å
i0	8108840.00
molecular_weight	20774.0 kDa
excluded_volume	25886 Å³
envelope_volume	29690 Å³
shell_volume	14945 Å³
envelope_diameter	59.9 Å
shell_rg	22.68 Å
envelope_rg	17.53 Å
shape_rg	17.32 Å
total_rg	18.42 Å
total_atoms	1466
n_residues	185
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	60.0 Å
rg_real	18.33 Å
rg_real_error	0.41 Å
i0_real	8.1090e+06
i0_real_error	1.0380e+05
rg_reciprocal	18.33 Å
i0_reciprocal	8109000.0000
total_estimate	0.8775
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	21.1 Å
skewness	0.390
kurtosis	-0.256
angular_range	— – 0.4350 Å⁻¹
current_alpha	0.0000
highest_alpha	1855000.0000
n_real_points	75
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.816; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.996; Smooth: 0.960

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (3)

Entity 1 polymer

description	GUANYL-SPECIFIC RIBONUCLEASE SA
formula_weight	10582.5 kDa
ec_number	3.1.27.3
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	1678

DVSGTVCLSALPPEATDTLNLIASDGPFPYSQDGVVFQNRESVLPTQSYGYYHEYTVITPGARTRGTRRIITGEATQEDYYTGDHYATFSLIDQTC

Source

organism	Streptomyces aureofaciens
ncbi_taxonomy_id	1894
host_organism	Escherichia coli

Entity 2 polymer

description	BARSTAR
formula_weight	10221.5 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

KKAVINGEQIRSISDLHQTLKKELALPEYYGENLDALWDCLTGWVEYPLVLEWRQFEQSKQLTENGAESVLQVFREAKAEGCDITIILS

Source

organism	Bacillus amyloliquefaciens
ncbi_taxonomy_id	1390
host_organism	Escherichia coli

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	190

▶

6. Citations (1)

▶

length_a	56.950 Å
length_b	56.950 Å
length_c	135.800 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 65
int_tables_number	170
crystal_system	—

resolution	1.700 Å
resolution_low	25.000 Å
r_factor	0.1620
r_work	0.1600
r_free	0.1900

fit	/app/data/saxs_computation/ay/1ay7/1ay7.fit
pddf	/app/data/pr_computation/ay/1ay7/1ay7.pr.out
profile	/app/data/saxs_computation/ay/1ay7/1ay7.dat

RIBONUCLEASE SA COMPLEX WITH BARSTAR

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Source

Entity 3 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)