BARNASE
Bacillus amyloliquefaciens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count | Chain A； UniProt 48–157 | Not recorded | BARSTAR × 1 （P11540） | X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions | Resolution 2.00 Å |
| 2 | Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count | Chain B； UniProt 48–157 | Not recorded | BARSTAR × 1 （P11540） | X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions | Resolution 2.00 Å |
| 3 | Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count | Chain C； UniProt 48–157 | Not recorded | BARSTAR × 1 （P11540） | X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions | Resolution 2.00 Å |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 1BRS | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1A2P BARNASE WILDTYPE STRUCTURE AT 1.5 ANGSTROMS RESOLUTION Deposited 1998-01-07 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 1.50 Å R-free 0.174 |
| 1A2P BARNASE WILDTYPE STRUCTURE AT 1.5 ANGSTROMS RESOLUTION Deposited 1998-01-07 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 1.50 Å R-free 0.174 |
| 1A2P BARNASE WILDTYPE STRUCTURE AT 1.5 ANGSTROMS RESOLUTION Deposited 1998-01-07 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；pH 7.5
|
Resolution 1.50 Å R-free 0.174 |
| 1B20 DELETION OF A BURIED SALT-BRIDGE IN BARNASE Deposited 1998-12-03 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Mutation：R69S | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；DROP: 8-12 MG/ML PROTEIN 6 MM ZNSO4 0.6 M (NH4)2SO4 WELL: 2.58-2.73 M AMMONIUM PHOSPHATE BUFFER, PH 7.5 1-2 MM ZNSO4 0.15-0.30 M (NH4)2SO4 5-10 MM NH4OH
|
Resolution 1.70 Å R-free 0.217 |
| 1B20 DELETION OF A BURIED SALT-BRIDGE IN BARNASE Deposited 1998-12-03 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Mutation：R69S | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；DROP: 8-12 MG/ML PROTEIN 6 MM ZNSO4 0.6 M (NH4)2SO4 WELL: 2.58-2.73 M AMMONIUM PHOSPHATE BUFFER, PH 7.5 1-2 MM ZNSO4 0.15-0.30 M (NH4)2SO4 5-10 MM NH4OH
|
Resolution 1.70 Å R-free 0.217 |
| 1B20 DELETION OF A BURIED SALT-BRIDGE IN BARNASE Deposited 1998-12-03 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Mutation：R69S | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；DROP: 8-12 MG/ML PROTEIN 6 MM ZNSO4 0.6 M (NH4)2SO4 WELL: 2.58-2.73 M AMMONIUM PHOSPHATE BUFFER, PH 7.5 1-2 MM ZNSO4 0.15-0.30 M (NH4)2SO4 5-10 MM NH4OH
|
Resolution 1.70 Å R-free 0.217 |
| 1B21 DELETION OF A BURIED SALT BRIDGE IN BARNASE Deposited 1998-12-03 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Mutation：R69S, D93N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；DROP: 8-12 MG/ML PROTEIN 6 MM ZNSO4 0.6 M (NH4)2SO4 WELL: 2.58-2.73 M AMMONIUM PHOSPHATE BUFFER, PH 7.5 1-2 MM ZNSO4 0.15-0.30 M (NH4)2SO4 5-10 MM NH4OH
|
Resolution 2.00 Å R-free 0.268 |
| 1B21 DELETION OF A BURIED SALT BRIDGE IN BARNASE Deposited 1998-12-03 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Mutation：R69S, D93N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；DROP: 8-12 MG/ML PROTEIN 6 MM ZNSO4 0.6 M (NH4)2SO4 WELL: 2.58-2.73 M AMMONIUM PHOSPHATE BUFFER, PH 7.5 1-2 MM ZNSO4 0.15-0.30 M (NH4)2SO4 5-10 MM NH4OH
|
Resolution 2.00 Å R-free 0.268 |
| 1B21 DELETION OF A BURIED SALT BRIDGE IN BARNASE Deposited 1998-12-03 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Mutation：R69S, D93N | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；DROP: 8-12 MG/ML PROTEIN 6 MM ZNSO4 0.6 M (NH4)2SO4 WELL: 2.58-2.73 M AMMONIUM PHOSPHATE BUFFER, PH 7.5 1-2 MM ZNSO4 0.15-0.30 M (NH4)2SO4 5-10 MM NH4OH
|
Resolution 2.00 Å R-free 0.268 |
| 1B27 STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-04 | Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；18-24% PEG-8K 0.2 M AMMONIUM SULPHATE 0.1 M TRIS PH8.0
|
Resolution 2.10 Å R-free 0.259 |
| 1B27 STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-04 | Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；18-24% PEG-8K 0.2 M AMMONIUM SULPHATE 0.1 M TRIS PH8.0
|
Resolution 2.10 Å R-free 0.259 |
| 1B27 STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-04 | Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；18-24% PEG-8K 0.2 M AMMONIUM SULPHATE 0.1 M TRIS PH8.0
|
Resolution 2.10 Å R-free 0.259 |
| 1B2S STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-11-30 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
48–157（110 aa）
|
Mutation：K27A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；18% PEG-4K 0.1M TRIS PH8.0 0.2M LI2SO4
|
Resolution 1.82 Å R-free 0.249 |
| 1B2S STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-11-30 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
48–157（110 aa）
|
Mutation：K27A | SO4 SULFATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；18% PEG-4K 0.1M TRIS PH8.0 0.2M LI2SO4
|
Resolution 1.82 Å R-free 0.249 |
| 1B2S STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-11-30 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
48–157（110 aa）
|
Mutation：K27A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；18% PEG-4K 0.1M TRIS PH8.0 0.2M LI2SO4
|
Resolution 1.82 Å R-free 0.249 |
| 1B2U STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-01 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
48–157（110 aa）
|
Mutation：K27A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；21% PEG-8K 0.2 M AMMONIUM SULPHATE 0.1 M NA CACODYLATE PH6.5
|
Resolution 2.10 Å R-free 0.276 |
| 1B2U STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-01 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
48–157（110 aa）
|
Mutation：K27A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；21% PEG-8K 0.2 M AMMONIUM SULPHATE 0.1 M NA CACODYLATE PH6.5
|
Resolution 2.10 Å R-free 0.276 |
| 1B2U STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-01 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
48–157（110 aa）
|
Mutation：K27A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；21% PEG-8K 0.2 M AMMONIUM SULPHATE 0.1 M NA CACODYLATE PH6.5
|
Resolution 2.10 Å R-free 0.276 |
| 1B2X BARNASE WILDTYPE STRUCTURE AT PH 7.5 FROM A CRYO_COOLED CRYSTAL AT 100K Deposited 1998-12-03 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；DROP: 8-12 MG/ML PROTEIN 6 MM ZNSO4 0.6 M (NH4)2SO4 WELL: 2.58-2.73 M AMMONIUM PHOSPHATE BUFFER, PH 7.5 1-2 MM ZNSO4 0.15-0.30 M (NH4)2SO4 5-10 MM NH4OH
|
Resolution 1.80 Å R-free 0.236 |
| 1B2X BARNASE WILDTYPE STRUCTURE AT PH 7.5 FROM A CRYO_COOLED CRYSTAL AT 100K Deposited 1998-12-03 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；DROP: 8-12 MG/ML PROTEIN 6 MM ZNSO4 0.6 M (NH4)2SO4 WELL: 2.58-2.73 M AMMONIUM PHOSPHATE BUFFER, PH 7.5 1-2 MM ZNSO4 0.15-0.30 M (NH4)2SO4 5-10 MM NH4OH
|
Resolution 1.80 Å R-free 0.236 |
| 1B2X BARNASE WILDTYPE STRUCTURE AT PH 7.5 FROM A CRYO_COOLED CRYSTAL AT 100K Deposited 1998-12-03 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；DROP: 8-12 MG/ML PROTEIN 6 MM ZNSO4 0.6 M (NH4)2SO4 WELL: 2.58-2.73 M AMMONIUM PHOSPHATE BUFFER, PH 7.5 1-2 MM ZNSO4 0.15-0.30 M (NH4)2SO4 5-10 MM NH4OH
|
Resolution 1.80 Å R-free 0.236 |
| 1B2Z DELETION OF A BURIED SALT BRIDGE IN BARNASE Deposited 1998-12-03 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Mutation：D93N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；DROP: 8-12 MG/ML PROTEIN 6 MM ZNSO4 0.6 M (NH4)2SO4 WELL: 2.58-2.73 M AMMONIUM PHOSPHATE BUFFER, PH 7.5 1-2 MM ZNSO4 0.15-0.30 M (NH4)2SO4 5-10 MM NH4OH
|
Resolution 2.03 Å R-free 0.228 |
| 1B2Z DELETION OF A BURIED SALT BRIDGE IN BARNASE Deposited 1998-12-03 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Mutation：D93N | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；DROP: 8-12 MG/ML PROTEIN 6 MM ZNSO4 0.6 M (NH4)2SO4 WELL: 2.58-2.73 M AMMONIUM PHOSPHATE BUFFER, PH 7.5 1-2 MM ZNSO4 0.15-0.30 M (NH4)2SO4 5-10 MM NH4OH
|
Resolution 2.03 Å R-free 0.228 |
| 1B2Z DELETION OF A BURIED SALT BRIDGE IN BARNASE Deposited 1998-12-03 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Mutation：D93N | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 7.5；DROP: 8-12 MG/ML PROTEIN 6 MM ZNSO4 0.6 M (NH4)2SO4 WELL: 2.58-2.73 M AMMONIUM PHOSPHATE BUFFER, PH 7.5 1-2 MM ZNSO4 0.15-0.30 M (NH4)2SO4 5-10 MM NH4OH
|
Resolution 2.03 Å R-free 0.228 |
| 1B3S STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-01 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
48–157（110 aa）
|
Mutation：H102A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；0.2M (NH4)2SO4; 0.1M TRIS/HCL PH 8.0: 22% PEG-8000
|
Resolution 2.39 Å R-free 0.324 |
| 1B3S STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-01 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
48–157（110 aa）
|
Mutation：H102A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；0.2M (NH4)2SO4; 0.1M TRIS/HCL PH 8.0: 22% PEG-8000
|
Resolution 2.39 Å R-free 0.324 |
| 1B3S STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE Deposited 1998-12-01 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
50–157（108 aa）
|
Mutation：H102A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；0.2M (NH4)2SO4; 0.1M TRIS/HCL PH 8.0: 22% PEG-8000
|
Resolution 2.39 Å R-free 0.324 |
| 1BAN THE CONTRIBUTION OF BURIED HYDROGEN BONDS TO PROTEIN STABILITY: THE CRYSTAL STRUCTURES OF TWO BARNASE MUTANTS Deposited 1993-05-19 | Different oligomeric state Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1BAN THE CONTRIBUTION OF BURIED HYDROGEN BONDS TO PROTEIN STABILITY: THE CRYSTAL STRUCTURES OF TWO BARNASE MUTANTS Deposited 1993-05-19 | Different oligomeric state Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1BAN THE CONTRIBUTION OF BURIED HYDROGEN BONDS TO PROTEIN STABILITY: THE CRYSTAL STRUCTURES OF TWO BARNASE MUTANTS Deposited 1993-05-19 | Different oligomeric state Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1BAO THE CONTRIBUTION OF BURIED HYDROGEN BONDS TO PROTEIN STABILITY: THE CRYSTAL STRUCTURES OF TWO BARNASE MUTANTS Deposited 1993-05-19 | Different oligomeric state Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1BAO THE CONTRIBUTION OF BURIED HYDROGEN BONDS TO PROTEIN STABILITY: THE CRYSTAL STRUCTURES OF TWO BARNASE MUTANTS Deposited 1993-05-19 | Different oligomeric state Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1BAO THE CONTRIBUTION OF BURIED HYDROGEN BONDS TO PROTEIN STABILITY: THE CRYSTAL STRUCTURES OF TWO BARNASE MUTANTS Deposited 1993-05-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1BGS RECOGNITION BETWEEN A BACTERIAL RIBONUCLEASE, BARNASE, AND ITS NATURAL INHIBITOR, BARSTAR Deposited 1993-11-02 | Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.60 Å |
| 1BGS RECOGNITION BETWEEN A BACTERIAL RIBONUCLEASE, BARNASE, AND ITS NATURAL INHIBITOR, BARSTAR Deposited 1993-11-02 | Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.60 Å |
| 1BGS RECOGNITION BETWEEN A BACTERIAL RIBONUCLEASE, BARNASE, AND ITS NATURAL INHIBITOR, BARSTAR Deposited 1993-11-02 | Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.60 Å |
| 1BNE BARNASE A43C/S80C DISULFIDE MUTANT Deposited 1995-03-31 | Different oligomeric state Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.10 Å |
| 1BNE BARNASE A43C/S80C DISULFIDE MUTANT Deposited 1995-03-31 | Different oligomeric state Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.10 Å |
| 1BNE BARNASE A43C/S80C DISULFIDE MUTANT Deposited 1995-03-31 | Different oligomeric state Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.10 Å |
| 1BNF BARNASE T70C/S92C DISULFIDE MUTANT Deposited 1995-03-31 | Different oligomeric state | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BNF BARNASE T70C/S92C DISULFIDE MUTANT Deposited 1995-03-31 | Different oligomeric state | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BNF BARNASE T70C/S92C DISULFIDE MUTANT Deposited 1995-03-31 | Different oligomeric state | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BNG BARNASE S85C/H102C DISULFIDE MUTANT Deposited 1995-03-31 | Different oligomeric state Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.10 Å |
| 1BNG BARNASE S85C/H102C DISULFIDE MUTANT Deposited 1995-03-31 | Different oligomeric state Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.10 Å |
| 1BNG BARNASE S85C/H102C DISULFIDE MUTANT Deposited 1995-03-31 | Different oligomeric state Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.10 Å |
| 1BNI BARNASE WILDTYPE STRUCTURE AT PH 6.0 Deposited 1995-05-17 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6；pH 6.0
|
Resolution 2.10 Å |
| 1BNI BARNASE WILDTYPE STRUCTURE AT PH 6.0 Deposited 1995-05-17 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6；pH 6.0
|
Resolution 2.10 Å |
| 1BNI BARNASE WILDTYPE STRUCTURE AT PH 6.0 Deposited 1995-05-17 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6；pH 6.0
|
Resolution 2.10 Å |
| 1BNJ BARNASE WILDTYPE STRUCTURE AT PH 9.0 Deposited 1995-05-17 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 9；pH 9.0
|
Resolution 2.10 Å |
| 1BNJ BARNASE WILDTYPE STRUCTURE AT PH 9.0 Deposited 1995-05-17 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 9；pH 9.0
|
Resolution 2.10 Å |
| 1BNJ BARNASE WILDTYPE STRUCTURE AT PH 9.0 Deposited 1995-05-17 | Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 9；pH 9.0
|
Resolution 2.10 Å |
| 1BNR BARNASE Deposited 1995-03-31 | Different oligomeric state Different experimental method Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | SOLUTION NMR mmCIF provides none of the parsed conditions | Resolution not provided |
| 1BNS STRUCTURAL STUDIES OF BARNASE MUTANTS Deposited 1994-04-11 | Different oligomeric state Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.05 Å |
| 1BNS STRUCTURAL STUDIES OF BARNASE MUTANTS Deposited 1994-04-11 | Different oligomeric state Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.05 Å |
| 1BNS STRUCTURAL STUDIES OF BARNASE MUTANTS Deposited 1994-04-11 | Different oligomeric state Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.05 Å |
| 1BRG CRYSTALLOGRAPHIC ANALYSIS OF PHE->LEU SUBSTITUTION IN THE HYDROPHOBIC CORE OF BARNASE Deposited 1994-03-30 | Different construct Different oligomeric state Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
50–157（108 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1BRG CRYSTALLOGRAPHIC ANALYSIS OF PHE->LEU SUBSTITUTION IN THE HYDROPHOBIC CORE OF BARNASE Deposited 1994-03-30 | Different construct Different oligomeric state Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
50–157（108 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1BRG CRYSTALLOGRAPHIC ANALYSIS OF PHE->LEU SUBSTITUTION IN THE HYDROPHOBIC CORE OF BARNASE Deposited 1994-03-30 | Different construct Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
50–157（108 aa）
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1BRH BARNASE MUTANT WITH LEU 14 REPLACED BY ALA Deposited 1995-03-09 | Different oligomeric state | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BRH BARNASE MUTANT WITH LEU 14 REPLACED BY ALA Deposited 1995-03-09 | Different oligomeric state | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BRH BARNASE MUTANT WITH LEU 14 REPLACED BY ALA Deposited 1995-03-09 | Different oligomeric state Different ligand/ion | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BRI BARNASE MUTANT WITH ILE 76 REPLACED BY ALA Deposited 1995-03-09 | Different oligomeric state Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 1BRI BARNASE MUTANT WITH ILE 76 REPLACED BY ALA Deposited 1995-03-09 | Different oligomeric state Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 1BRI BARNASE MUTANT WITH ILE 76 REPLACED BY ALA Deposited 1995-03-09 | Different oligomeric state Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 1BRJ BARNASE MUTANT WITH ILE 88 REPLACED BY ALA Deposited 1995-03-09 | Different oligomeric state | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BRJ BARNASE MUTANT WITH ILE 88 REPLACED BY ALA Deposited 1995-03-09 | Different oligomeric state | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BRJ BARNASE MUTANT WITH ILE 88 REPLACED BY ALA Deposited 1995-03-09 | Different oligomeric state Different ligand/ion | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BRK BARNASE MUTANT WITH ILE 96 REPLACED BY ALA Deposited 1995-03-09 | Different oligomeric state Different ligand/ion | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BRK BARNASE MUTANT WITH ILE 96 REPLACED BY ALA Deposited 1995-03-09 | Different oligomeric state Different ligand/ion | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BRK BARNASE MUTANT WITH ILE 96 REPLACED BY ALA Deposited 1995-03-09 | Different oligomeric state Different ligand/ion | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | ZN ZINC ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BRN SUBSITE BINDING IN AN RNASE: STRUCTURE OF A BARNASE-TETRANUCLEOTIDE COMPLEX AT 1.76 ANGSTROMS RESOLUTION Deposited 1993-11-17 | Different oligomeric state Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric |
Chain L
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.76 Å |
| 1BRN SUBSITE BINDING IN AN RNASE: STRUCTURE OF A BARNASE-TETRANUCLEOTIDE COMPLEX AT 1.76 ANGSTROMS RESOLUTION Deposited 1993-11-17 | Different oligomeric state Different structure-quality metrics | Assembly 2 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric |
Chain M
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.76 Å |
| 1BSA CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE Deposited 1993-07-19 | Different oligomeric state | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BSA CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE Deposited 1993-07-19 | Different oligomeric state | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BSA CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE Deposited 1993-07-19 | Different oligomeric state | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BSB CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE Deposited 1993-07-19 | Different oligomeric state | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BSB CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE Deposited 1993-07-19 | Different oligomeric state | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BSB CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE Deposited 1993-07-19 | Different oligomeric state | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BSC CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE Deposited 1993-07-19 | Different oligomeric state | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BSC CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE Deposited 1993-07-19 | Different oligomeric state | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BSC CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE Deposited 1993-07-19 | Different oligomeric state | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BSD CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE Deposited 1993-07-19 | Different oligomeric state Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.30 Å |
| 1BSD CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE Deposited 1993-07-19 | Different oligomeric state Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.30 Å |
| 1BSD CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE Deposited 1993-07-19 | Different oligomeric state Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.30 Å |
| 1BSE CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE Deposited 1993-07-19 | Different oligomeric state | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BSE CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE Deposited 1993-07-19 | Different oligomeric state | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1BSE CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE Deposited 1993-07-19 | Different oligomeric state | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.00 Å |
| 1FW7 NMR STRUCTURE OF 15N-LABELED BARNASE Deposited 2000-09-22 | Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.5；303 K；Ionic strength (raw mmCIF value) 0.04；Pressure 1
NMR sample composition
15N-labeled barnase, 1mM | 10mM potassium-phosphate buffer
in 90% H20/10% D2O
NMR sample composition
15N-labeled barnase, 1mM | 10mM potassium-phosphate buffer
in D2O
|
Resolution not provided |
| 1RNB CRYSTAL STRUCTURE OF A BARNASE-D(*GP*C) COMPLEX AT 1.9 ANGSTROMS RESOLUTION Deposited 1991-03-19 | Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein–DNA Monomer；Protein × 1 PDB declaration: dimeric |
Chain A
48–157（110 aa）
|
Not recorded | SO4 SULFATE ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 1X1U Water-mediate interaction at aprotein-protein interface Deposited 2005-04-13 | Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.30 Å R-free 0.270 |
| 1X1U Water-mediate interaction at aprotein-protein interface Deposited 2005-04-13 | Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.30 Å R-free 0.270 |
| 1X1U Water-mediate interaction at aprotein-protein interface Deposited 2005-04-13 | Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.30 Å R-free 0.270 |
| 1X1W Water-mediate interaction at aprotein-protein interface Deposited 2005-04-14 | Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.10 Å R-free 0.265 |
| 1X1W Water-mediate interaction at aprotein-protein interface Deposited 2005-04-14 | Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.10 Å R-free 0.265 |
| 1X1W Water-mediate interaction at aprotein-protein interface Deposited 2005-04-14 | Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 2.10 Å R-free 0.265 |
| 1X1X Water-mediate interaction at aprotein-protein interface Deposited 2005-04-15 | Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.30 Å R-free 0.237 |
| 1X1X Water-mediate interaction at aprotein-protein interface Deposited 2005-04-15 | Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.30 Å R-free 0.237 |
| 1X1X Water-mediate interaction at aprotein-protein interface Deposited 2005-04-15 | Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
48–157（110 aa）
|
Not recorded | No recorded non-water small molecule | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.30 Å R-free 0.237 |
| 1X1Y Water-mediate interaction at aprotein-protein interface Deposited 2005-04-15 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
48–157（110 aa）
|
Mutation：Q2A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.90 Å R-free 0.250 |
| 1X1Y Water-mediate interaction at aprotein-protein interface Deposited 2005-04-15 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
48–157（110 aa）
|
Mutation：Q2A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.90 Å R-free 0.250 |
| 1X1Y Water-mediate interaction at aprotein-protein interface Deposited 2005-04-15 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
48–157（110 aa）
|
Mutation：Q2A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP
|
Resolution 1.90 Å R-free 0.250 |
| 1YVS Trimeric domain swapped barnase Deposited 1998-12-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
48–157（110 aa）
|
Not recorded | SO4 SULFATE ION × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 4.5；pH 4.5
|
Resolution 2.20 Å R-free 0.244 |
| 1YVS Trimeric domain swapped barnase Deposited 1998-12-10 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 6 PDB declaration: hexameric |
Chain A
48–157（110 aa）
|
Not recorded | SO4 SULFATE ION × 12 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 4.5；pH 4.5
|
Resolution 2.20 Å R-free 0.244 |
| 2C4B Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102A Deposited 2005-10-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
49–157（109 aa）
|
Mutation：YES | 2PE NONAETHYLENE GLYCOL × 2 GOL GLYCEROL × 1 EDO 1,2-ETHANEDIOL × 4 FMT FORMIC ACID × 2 SO4 SULFATE ION × 7 UNX UNKNOWN LIGAND × 15 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；1.3 M AMMONIUM SULPHATE, 7% PEG400 (V/V), 0.1 M MES PH 6.5 AS RESERVOIR SOLUTION. DROPLETS MIXED FROM 8 UL PROTEIN (30 MG/ML) AND 4 UL RESERVOIR. SITTING DROP VAPOR DIFFUSION. 4 DEG. C.
|
Resolution 1.30 Å R-free 0.164 |
| 2C4B Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102A Deposited 2005-10-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
49–157（109 aa）
|
Mutation：YES | 2PE NONAETHYLENE GLYCOL × 2 EDO 1,2-ETHANEDIOL × 6 FMT FORMIC ACID × 1 SO4 SULFATE ION × 10 MES 2-(N-MORPHOLINO)-ETHANESULFONIC ACID × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；277 K；1.3 M AMMONIUM SULPHATE, 7% PEG400 (V/V), 0.1 M MES PH 6.5 AS RESERVOIR SOLUTION. DROPLETS MIXED FROM 8 UL PROTEIN (30 MG/ML) AND 4 UL RESERVOIR. SITTING DROP VAPOR DIFFUSION. 4 DEG. C.
|
Resolution 1.30 Å R-free 0.164 |
| 2F4Y Barnase cross-linked with glutaraldehyde Deposited 2005-11-24 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
50–157（108 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；300 K；AMMONIUM SULFATE, ZINC CHLORIDE, HEPES BUFFER
PROTEIN AT 20MG/ML, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 2.15 Å |
| 2F4Y Barnase cross-linked with glutaraldehyde Deposited 2005-11-24 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
50–157（108 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；300 K；AMMONIUM SULFATE, ZINC CHLORIDE, HEPES BUFFER
PROTEIN AT 20MG/ML, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 2.15 Å |
| 2F4Y Barnase cross-linked with glutaraldehyde Deposited 2005-11-24 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
50–157（108 aa）
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；300 K；AMMONIUM SULFATE, ZINC CHLORIDE, HEPES BUFFER
PROTEIN AT 20MG/ML, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 2.15 Å |
| 2F56 Barnase cross-linked with glutaraldehyde soaked in 6M urea Deposited 2005-11-25 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
50–157（108 aa）
|
Not recorded | URE UREA × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；300 K；AMMONIUM SULFATE, HEPES BUFFER. PROTEIN 20 MG/ML, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 1.96 Å |
| 2F56 Barnase cross-linked with glutaraldehyde soaked in 6M urea Deposited 2005-11-25 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
50–157（108 aa）
|
Not recorded | URE UREA × 6 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；300 K；AMMONIUM SULFATE, HEPES BUFFER. PROTEIN 20 MG/ML, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 1.96 Å |
| 2F56 Barnase cross-linked with glutaraldehyde soaked in 6M urea Deposited 2005-11-25 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
50–157（108 aa）
|
Not recorded | URE UREA × 2 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；300 K；AMMONIUM SULFATE, HEPES BUFFER. PROTEIN 20 MG/ML, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 1.96 Å |
| 2F56 Barnase cross-linked with glutaraldehyde soaked in 6M urea Deposited 2005-11-25 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
50–157（108 aa）
Chain C
50–157（108 aa）
|
Not recorded | URE UREA × 5 ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；300 K；AMMONIUM SULFATE, HEPES BUFFER. PROTEIN 20 MG/ML, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 1.96 Å |
| 2F5M Cross-linked barnase soaked in bromo-ethanol Deposited 2005-11-26 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
50–157（108 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；300 K；FROM AMMONIUM SULFATE, PROTEIN =20 MG/ML, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 1.95 Å |
| 2F5M Cross-linked barnase soaked in bromo-ethanol Deposited 2005-11-26 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
50–157（108 aa）
|
Not recorded | BRJ 2-BROMOETHANOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；300 K；FROM AMMONIUM SULFATE, PROTEIN =20 MG/ML, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 1.95 Å |
| 2F5M Cross-linked barnase soaked in bromo-ethanol Deposited 2005-11-26 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
50–157（108 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；300 K；FROM AMMONIUM SULFATE, PROTEIN =20 MG/ML, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 1.95 Å |
| 2F5W Cross-linked barnase soaked in 3 M thiourea Deposited 2005-11-28 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
50–157（108 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；300 K；PROTEIN 20 MG/ML, AMMONIUM SULFATE, ZINC CHLORIDE, HEPES BUFFER, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 2.00 Å |
| 2F5W Cross-linked barnase soaked in 3 M thiourea Deposited 2005-11-28 | Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
50–157（108 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；300 K；PROTEIN 20 MG/ML, AMMONIUM SULFATE, ZINC CHLORIDE, HEPES BUFFER, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 2.00 Å |
| 2F5W Cross-linked barnase soaked in 3 M thiourea Deposited 2005-11-28 | Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
50–157（108 aa）
|
Not recorded | ZN ZINC ION × 1 TOU THIOUREA × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；300 K；PROTEIN 20 MG/ML, AMMONIUM SULFATE, ZINC CHLORIDE, HEPES BUFFER, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 2.00 Å |
| 2KF3 Barnase, low pressure reference NMR structure Deposited 2009-02-11 | Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Mutation：H102A | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.7；298 K；Pressure 3
NMR sample composition
1.1 mM [U-100% 13C; U-100% 15N] barnase, 30 mM sodium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2KF4 Barnase high pressure structure Deposited 2009-02-11 | Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Mutation：H102A | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.7；298 K；Pressure 200
NMR sample composition
1.1 mM [U-100% 13C; U-100% 15N] barnase, 30 mM sodium phosphate, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2KF5 Barnase bound to d(CGAC), low pressure Deposited 2009-02-11 | Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Mutation：H102A | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.7；298 K；Pressure 3
NMR sample composition
1.1 mM [U-100% 13C; U-100% 15N] barnase, 30 mM sodium phosphate, 4.7 mM d(CGAC), 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2KF6 Barnase bound to d(CGAC) high pressure Deposited 2009-02-11 | Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Mutation：H102A | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6.7；298 K；Pressure 200
NMR sample composition
1.1 mM [U-100% 13C; U-100% 15N] barnase, 30 mM sodium phosphate, 4.7 mM d(CGAC), 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2ZA4 Crystal Structural Analysis of Barnase-barstar Complex Deposited 2007-10-01 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
48–157（110 aa）
|
Mutation：K98A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20% PEG 6000, 0.1M HEPES (pH 7.0), 1.0M lithium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.58 Å R-free 0.200 |
| 2ZA4 Crystal Structural Analysis of Barnase-barstar Complex Deposited 2007-10-01 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
48–157（110 aa）
|
Mutation：K98A | CL CHLORIDE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20% PEG 6000, 0.1M HEPES (pH 7.0), 1.0M lithium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.58 Å R-free 0.200 |
| 2ZA4 Crystal Structural Analysis of Barnase-barstar Complex Deposited 2007-10-01 | Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–157（110 aa）
|
Mutation：K98A | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20% PEG 6000, 0.1M HEPES (pH 7.0), 1.0M lithium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.58 Å R-free 0.200 |
| 2ZA4 Crystal Structural Analysis of Barnase-barstar Complex Deposited 2007-10-01 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
48–157（110 aa）
|
Mutation：K98A | CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；20% PEG 6000, 0.1M HEPES (pH 7.0), 1.0M lithium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.58 Å R-free 0.200 |
| 3KCH Baranase crosslinked by glutaraldehyde Deposited 2009-10-21 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
48–157（110 aa）
|
Not recorded | PTD PENTANEDIAL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；290 K；ammonium sulfate, PEG 1000, HEPES, glutaraldehyde, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 1.94 Å R-free 0.185 |
| 3KCH Baranase crosslinked by glutaraldehyde Deposited 2009-10-21 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
48–157（110 aa）
|
Not recorded | PTD PENTANEDIAL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；290 K；ammonium sulfate, PEG 1000, HEPES, glutaraldehyde, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 1.94 Å R-free 0.185 |
| 3KCH Baranase crosslinked by glutaraldehyde Deposited 2009-10-21 | Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain C
48–157（110 aa）
|
Not recorded | PTD PENTANEDIAL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；290 K；ammonium sulfate, PEG 1000, HEPES, glutaraldehyde, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K
|
Resolution 1.94 Å R-free 0.185 |
| 3Q3F Engineering Domain-Swapped Binding Interfaces by Mutually Exclusive Folding: Insertion of Ubiquitin into position 103 of Barnase Deposited 2010-12-21 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
48–150（103 aa）
Fragment：UNP P00648 48-150 and 151-157, UNP P0CG48 residues 2-76
Chain A
151–157（7 aa）
Fragment：UNP P00648 48-150 and 151-157, UNP P0CG48 residues 2-76
|
Not recorded | SO4 SULFATE ION × 16 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；10 mM Tris (pH 8.0), 1 M (NH4)2SO4, 1.5% isopropanol (v/v), VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.17 Å R-free 0.233 |
| 6PQK Cryogenic crystal structure of barnase A43C/S80C bound to barstar C40A/S59C/A67C/C82A Deposited 2019-07-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
48–157（110 aa）
|
Mutation：A43C, S80C | PO4 PHOSPHATE ION × 3 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；50mM NaPO4 pH 6.5, 25% PEG 8K, 0.1M AmSO4
4uL protein at 10-15 mg/mL in H2O + 4uL motherliquor per hanging drop, over 1 mL motherliquor in well.
cryoprotectant used before freezing was: 12.5% glycerol + 12.5% ethylene glycol
|
Resolution 1.20 Å R-free 0.160 |
| 6PQK Cryogenic crystal structure of barnase A43C/S80C bound to barstar C40A/S59C/A67C/C82A Deposited 2019-07-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
48–157（110 aa）
|
Mutation：A43C, S80C | PO4 PHOSPHATE ION × 1 EDO 1,2-ETHANEDIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；298 K；50mM NaPO4 pH 6.5, 25% PEG 8K, 0.1M AmSO4
4uL protein at 10-15 mg/mL in H2O + 4uL motherliquor per hanging drop, over 1 mL motherliquor in well.
cryoprotectant used before freezing was: 12.5% glycerol + 12.5% ethylene glycol
|
Resolution 1.20 Å R-free 0.160 |
| 7MRX Cryogenic crystal structure of barnase A43C/S80C bound to barstar C40A/C82A Deposited 2021-05-10 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain A
48–157（110 aa）
|
Mutation：A43C, S80C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；50mM NaPO4, 25% PEG 8K, 0.1M AmSO4 4uL protein at 10-15 mg/mL in H2O + 4uL motherliquor per hanging drop, over 1 mL motherliquor in well. cryoprotectant used before freezing was: 25% MPD
|
Resolution 2.29 Å R-free 0.229 |
| 7MRX Cryogenic crystal structure of barnase A43C/S80C bound to barstar C40A/C82A Deposited 2021-05-10 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
48–157（110 aa）
|
Mutation：A43C, S80C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；50mM NaPO4, 25% PEG 8K, 0.1M AmSO4 4uL protein at 10-15 mg/mL in H2O + 4uL motherliquor per hanging drop, over 1 mL motherliquor in well. cryoprotectant used before freezing was: 25% MPD
|
Resolution 2.29 Å R-free 0.229 |
| 7MRX Cryogenic crystal structure of barnase A43C/S80C bound to barstar C40A/C82A Deposited 2021-05-10 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain E
48–157（110 aa）
|
Mutation：A43C, S80C | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；50mM NaPO4, 25% PEG 8K, 0.1M AmSO4 4uL protein at 10-15 mg/mL in H2O + 4uL motherliquor per hanging drop, over 1 mL motherliquor in well. cryoprotectant used before freezing was: 25% MPD
|
Resolution 2.29 Å R-free 0.229 |
51 other PDB entries and 135 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | RNBR_BACAM |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–110； UniProt 48–157 Author chain B； PDBConstruct 1–110； UniProt 48–157 Author chain C； PDBConstruct 1–110； UniProt 48–157 |
