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1bff

THE 154 AMINO ACID FORM OF HUMAN BASIC FIBROBLAST GROWTH FACTOR

Method: X-RAY DIFFRACTION D_max: 44.8 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1bff
deposition_date	1996-12-06
title	THE 154 AMINO ACID FORM OF HUMAN BASIC FIBROBLAST GROWTH FACTOR
keywords	GROWTH FACTOR; GROWTH FACTOR
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	14.94 Å
rg_electron	13.47 Å
i0	4545160.00
molecular_weight	14958.0 kDa
excluded_volume	18667 Å³
envelope_volume	20382 Å³
shell_volume	12590 Å³
envelope_diameter	43.6 Å
shell_rg	19.60 Å
envelope_rg	13.79 Å
shape_rg	13.41 Å
total_rg	14.88 Å
total_atoms	1048
n_residues	129
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	44.8 Å
rg_real	14.79 Å
rg_real_error	0.19 Å
i0_real	4.5450e+06
i0_real_error	5.0020e+04
rg_reciprocal	14.80 Å
i0_reciprocal	4545000.0000
total_estimate	0.8931
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	21.0 Å
skewness	0.004
kurtosis	-0.429
angular_range	— – 0.5000 Å⁻¹
current_alpha	0.0000
highest_alpha	1267000.0000
n_real_points	80
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.891; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.977; Smooth: 0.955

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	BASIC FIBROBLAST GROWTH FACTOR
formula_weight	14800.1 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	1115

KDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 2 non-polymer

description	PHOSPHATE ION
formula_weight	95.0 kDa
src_method	syn
entity_count	1

Entity 3 non-polymer

description	BETA-MERCAPTOETHANOL
formula_weight	78.1 kDa
src_method	syn
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	67

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6. Citations (1)

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length_a	30.800 Å
length_b	33.300 Å
length_c	36.500 Å
angle_alpha	64.10 °
angle_beta	73.00 °
angle_gamma	76.10 °
z_pdb	1

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	2.000 Å
resolution_low	20.000 Å
r_factor	—
r_work	0.1900
r_free	—

fit	/app/data/saxs_computation/bf/1bff/1bff.fit
pddf	/app/data/pr_computation/bf/1bff/1bff.pr.out
profile	/app/data/saxs_computation/bf/1bff/1bff.dat

THE 154 AMINO ACID FORM OF HUMAN BASIC FIBROBLAST GROWTH FACTOR

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction