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1bsv

GDP-FUCOSE SYNTHETASE FROM ESCHERICHIA COLI COMPLEX WITH NADPH

Method: X-RAY DIFFRACTION D_max: 67.7 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1bsv
deposition_date	1998-08-31
title	GDP-FUCOSE SYNTHETASE FROM ESCHERICHIA COLI COMPLEX WITH NADPH
keywords	EPIMERASE-REDUCTASE, NADPH, GDP-FUCOSE, OXIDOREDUCTASE; OXIDOREDUCTASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	20.83 Å
rg_electron	19.80 Å
i0	23614800.00
molecular_weight	36192.0 kDa
excluded_volume	44903 Å³
envelope_volume	52351 Å³
shell_volume	22016 Å³
envelope_diameter	68.0 Å
shell_rg	26.50 Å
envelope_rg	19.95 Å
shape_rg	19.82 Å
total_rg	20.59 Å
total_atoms	2546
n_residues	317
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	67.7 Å
rg_real	20.77 Å
rg_real_error	0.36 Å
i0_real	2.3610e+07
i0_real_error	2.5500e+05
rg_reciprocal	20.78 Å
i0_reciprocal	23610000.0000
total_estimate	0.8816
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	25.5 Å
skewness	0.315
kurtosis	-0.195
angular_range	— – 0.3800 Å⁻¹
current_alpha	0.0000
highest_alpha	4954000.0000
n_real_points	70
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.829; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.973

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	PROTEIN (GDP-FUCOSE SYNTHETASE)
formula_weight	36187.1 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2630

MSKQRVFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDSRAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKLLFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRSVMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMAAASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASKPDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG

Source

organism	Escherichia coli
ncbi_taxonomy_id	83333
host_organism	Escherichia coli BL21

Entity 2 non-polymer

description	NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
formula_weight	745.4 kDa
src_method	syn
entity_count	1

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	84

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6. Citations (1)

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length_a	104.300 Å
length_b	104.300 Å
length_c	74.900 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 32 2 1
int_tables_number	154
crystal_system	—

resolution	2.200 Å
resolution_low	14.000 Å
r_factor	0.1700
r_work	—
r_free	—

fit	/app/data/saxs_computation/bs/1bsv/1bsv.fit
pddf	/app/data/pr_computation/bs/1bsv/1bsv.pr.out
profile	/app/data/saxs_computation/bs/1bsv/1bsv.dat

GDP-FUCOSE SYNTHETASE FROM ESCHERICHIA COLI COMPLEX WITH NADPH

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction