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1bw8

MU2 ADAPTIN SUBUNIT (AP50) OF AP2 ADAPTOR (SECOND DOMAIN), COMPLEXED WITH EGFR INTERNALIZATION PEPTIDE FYRALM

Method: X-RAY DIFFRACTION D_max: 87.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1bw8
deposition_date	1998-09-30
title	MU2 ADAPTIN SUBUNIT (AP50) OF AP2 ADAPTOR (SECOND DOMAIN), COMPLEXED WITH EGFR INTERNALIZATION PEPTIDE FYRALM
keywords	ENDOCYTOSIS, ADAPTOR, PEPTIDE COMPLEX, PEPTIDE BINDING PROTEIN; PEPTIDE BINDING PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	24.05 Å
rg_electron	23.59 Å
i0	14338100.00
molecular_weight	29802.0 kDa
excluded_volume	37947 Å³
envelope_volume	46832 Å³
shell_volume	18084 Å³
envelope_diameter	88.8 Å
shell_rg	28.49 Å
envelope_rg	24.13 Å
shape_rg	23.58 Å
total_rg	24.33 Å
total_atoms	2092
n_residues	262
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	87.5 Å
rg_real	24.37 Å
rg_real_error	0.84 Å
i0_real	1.4340e+07
i0_real_error	2.0240e+05
rg_reciprocal	24.30 Å
i0_reciprocal	14340000.0000
total_estimate	0.7639
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	20.5 Å
skewness	0.608
kurtosis	-0.226
angular_range	— – 0.3300 Å⁻¹
current_alpha	0.0000
highest_alpha	5099000.0000
n_real_points	65
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.537; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.404; Smooth: 0.913

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	PROTEIN (MU2 ADAPTIN SUBUNIT)
formula_weight	36706.6 kDa
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	2143

MHHHHHHQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC

Source

organism	Rattus norvegicus
common_name	Norway rat
ncbi_taxonomy_id	10116
host_organism	Escherichia coli BL21

Entity 2 polymer

description	PROTEIN (INTERNALIZATION SIGNAL FROM EGFR)
formula_weight	801.0 kDa
src_method	syn
entity_count	1

Polymer

type	polypeptide(L)
strand_id	P

FYRALM

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	51

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6. Citations (1)

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length_a	125.680 Å
length_b	125.680 Å
length_c	73.160 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 64
int_tables_number	172
crystal_system	—

resolution	2.650 Å
resolution_low	35.000 Å
r_factor	0.2720
r_work	0.2500
r_free	0.2960

fit	/app/data/saxs_computation/bw/1bw8/1bw8.fit
pddf	/app/data/pr_computation/bw/1bw8/1bw8.pr.out
profile	/app/data/saxs_computation/bw/1bw8/1bw8.dat

MU2 ADAPTIN SUBUNIT (AP50) OF AP2 ADAPTOR (SECOND DOMAIN), COMPLEXED WITH EGFR INTERNALIZATION PEPTIDE FYRALM

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Entity 3 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)