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1cew

;THE 2.0 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF CHICKEN EGG WHITE CYSTATIN AND ITS POSSIBLE MODE OF INTERACTION WITH CYSTEINE PROTEINASES ;

Method: X-RAY DIFFRACTION D_max: 52.0 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1cew
deposition_date	1993-04-21
title	;THE 2.0 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF CHICKEN EGG WHITE CYSTATIN AND ITS POSSIBLE MODE OF INTERACTION WITH CYSTEINE PROTEINASES ;
keywords	PROTEINASE INHIBITOR(CYSTEINE); PROTEINASE INHIBITOR(CYSTEINE)
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	15.74 Å
rg_electron	14.79 Å
i0	3167390.00
molecular_weight	12191.0 kDa
excluded_volume	15186 Å³
envelope_volume	18351 Å³
shell_volume	11075 Å³
envelope_diameter	50.0 Å
shell_rg	19.61 Å
envelope_rg	14.93 Å
shape_rg	14.79 Å
total_rg	15.83 Å
total_atoms	854
n_residues	108
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	52.0 Å
rg_real	15.71 Å
rg_real_error	0.28 Å
i0_real	3.1670e+06
i0_real_error	3.3600e+04
rg_reciprocal	15.71 Å
i0_reciprocal	3167000.0000
total_estimate	0.8909
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	17.8 Å
skewness	0.268
kurtosis	-0.358
angular_range	— – 0.5000 Å⁻¹
current_alpha	0.0000
highest_alpha	511700.0000
n_real_points	80
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.874; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.986; Smooth: 0.970

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

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6. Citations (7)

The 2.0 A X-ray crystal structure of chicken egg white cystatin and its possible mode of interaction with cysteine proteinases.

EMBO J. (1988)

PMID: 3191914 PRIMARY

Conformational Variability of Chicken Cystatin: Comparison of Structures Determined by X-Ray Diffraction and NMR-Spectroscopy

J.Mol.Biol. (1993)

The Structures of Native Phosphorylated Chicken Cystatin and of a Recombinant Unphosphorylated Variant in Solution

J.Mol.Biol. (1993)

The Cystatins: Protein Inhibitors of Cysteine Proteinases

FEBS Lett. (1991)

;Mechanism of Interaction of Cysteine Proteinases and Their Protein Inhibitors as Compared to the Serine Proteinase-Inhibitor Interaction ;

Biol.Chem.Hoppe-Seyler (1990)

;Mechanism of Inhibition of Papain by Chicken Egg White Cystatin: Inhibition Constants of N-Terminally Truncated Forms and Cyanogen Bromide Fragments of the Inhibitor ;

FEBS Lett. (1989)

The Cysteine Proteinase Inhibitor Chicken Cystatin is a Phophoprotein

FEBS Lett. (1989)

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length_a	47.900 Å
length_b	47.900 Å
length_c	87.500 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 31 2 1
int_tables_number	152
crystal_system	—

resolution	2.000 Å
resolution_low	— Å
r_factor	—
r_work	0.1980
r_free	—

description	CYSTATIN
formula_weight	12204.9 kDa
src_method	man
entity_count	1

type	polypeptide(L)
strand_id	I
atom_count	977

;THE 2.0 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF CHICKEN EGG WHITE CYSTATIN AND ITS POSSIBLE MODE OF INTERACTION WITH CYSTEINE PROTEINASES ;

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 water

6. Citations (7)

7. Files & Curves (10)

Files (7)

Curves (3)

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	123

fit	/app/data/saxs_computation/ce/1cew/1cew.fit
pddf	/app/data/pr_computation/ce/1cew/1cew.pr.out
profile	/app/data/saxs_computation/ce/1cew/1cew.dat

organism	Gallus gallus
common_name	chicken
ncbi_taxonomy_id	9031