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1cjf

PROFILIN BINDS PROLINE-RICH LIGANDS IN TWO DISTINCT AMIDE BACKBONE ORIENTATIONS

Method: X-RAY DIFFRACTION D_max: 80.6 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1cjf
deposition_date	1999-04-13
title	PROFILIN BINDS PROLINE-RICH LIGANDS IN TWO DISTINCT AMIDE BACKBONE ORIENTATIONS
keywords	PROFILIN, ACTIN-BINDING PROTEIN, CYTOSKELETON, POLY-L-PROLINE, STRUCTURAL REGULATION PROTEIN; STRUCTURAL REGULATION PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	24.61 Å
rg_electron	24.14 Å
i0	18429600.00
molecular_weight	32998.0 kDa
excluded_volume	41487 Å³
envelope_volume	49958 Å³
shell_volume	18366 Å³
envelope_diameter	82.8 Å
shell_rg	29.69 Å
envelope_rg	24.18 Å
shape_rg	24.14 Å
total_rg	24.89 Å
total_atoms	2319
n_residues	306
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	80.6 Å
rg_real	24.83 Å
rg_real_error	0.54 Å
i0_real	1.8430e+07
i0_real_error	2.7610e+05
rg_reciprocal	24.78 Å
i0_reciprocal	18430000.0000
total_estimate	0.8250
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	21.7 Å
skewness	0.456
kurtosis	-0.592
angular_range	— – 0.3250 Å⁻¹
current_alpha	0.0000
highest_alpha	7471000.0000
n_real_points	64
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.695; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.690; Smooth: 0.944

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	PROTEIN (HUMAN PLATELET PROFILIN)
formula_weight	14940.0 kDa
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	2343

AGWNAYIDNLMADGTCQDAAIVGYKDSPSVWAAVPGKTFVNITPAEVGVLVGKDRSSFYVNGLTLGGQKCSVIRDSLLQDGEFSMDLRTKSTGGAPTFNVTVTKTDKTLVLLMGKEGVHGGLINKKCYEMASHLRRSQY

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606

Entity 2 polymer

description	PROTEIN (PROLINE PEPTIDE)
formula_weight	1474.7 kDa
src_method	syn
entity_count	2

Polymer

type	polypeptide(L)
strand_id	C,D

PPPPPPPPPPPPPPP

Entity 3 non-polymer

description	7-HYDROXY-4-METHYL-3-(2-HYDROXY-ETHYL)COUMARIN
formula_weight	220.2 kDa
src_method	syn
entity_count	2

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	24

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6. Citations (1)

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length_a	105.400 Å
length_b	51.800 Å
length_c	77.500 Å
angle_alpha	90.00 °
angle_beta	131.10 °
angle_gamma	90.00 °
z_pdb	8

space_group	C 1 2 1
int_tables_number	5
crystal_system	—

resolution	2.300 Å
resolution_low	8.000 Å
r_factor	—
r_work	0.1950
r_free	0.2930

fit	/app/data/saxs_computation/cj/1cjf/1cjf.fit
pddf	/app/data/pr_computation/cj/1cjf/1cjf.pr.out
profile	/app/data/saxs_computation/cj/1cjf/1cjf.dat

PROFILIN BINDS PROLINE-RICH LIGANDS IN TWO DISTINCT AMIDE BACKBONE ORIENTATIONS

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Source

Entity 2 polymer

Polymer

Entity 3 non-polymer

Entity 4 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)