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1do0

ORTHORHOMBIC CRYSTAL FORM OF HEAT SHOCK LOCUS U (HSLU) FROM ESCHERICHIA COLI

Method: X-RAY DIFFRACTION D_max: 138.0 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1do0
deposition_date	1999-12-18
title	ORTHORHOMBIC CRYSTAL FORM OF HEAT SHOCK LOCUS U (HSLU) FROM ESCHERICHIA COLI
keywords	HSLU, CLPY, AAA-ATPASE, ATP-DEPENDENT PROTEOLYSIS, ATPASE, PROTEASOME, CHAPERONE; CHAPERONE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	45.19 Å
rg_electron	44.24 Å
i0	1151360000.00
molecular_weight	275970.0 kDa
excluded_volume	344230 Å³
envelope_volume	498210 Å³
shell_volume	89241 Å³
envelope_diameter	141.3 Å
shell_rg	52.88 Å
envelope_rg	43.47 Å
shape_rg	44.27 Å
total_rg	44.48 Å
total_atoms	19366
n_residues	2436
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	138.0 Å
rg_real	44.87 Å
rg_real_error	0.86 Å
i0_real	1.1510e+09
i0_real_error	1.8600e+07
rg_reciprocal	45.19 Å
i0_reciprocal	1152000000.0000
total_estimate	0.8978
solution_quality	GOOD a GOOD solution
n_peaks	0
r_peak_primary	— Å
skewness	0.029
kurtosis	-0.590
angular_range	— – 0.1750 Å⁻¹
current_alpha	0.0000
highest_alpha	154000000.0000
n_real_points	36
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.937; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.974; Smooth: 0.884

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	PROTEIN (HEAT SHOCK LOCUS U)
formula_weight	49528.5 kDa
src_method	man
entity_count	6

Polymer

type	polypeptide(L)
strand_id	A,B,C,D,E,F
atom_count	19366

SEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEELRHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRDLTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFRKKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKIKDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREGVQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQALTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGARRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL

Source

organism	Escherichia coli
ncbi_taxonomy_id	562
host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

description	ADENOSINE-5'-TRIPHOSPHATE
formula_weight	507.2 kDa
src_method	syn
entity_count	4

Entity 3 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	2

Entity 4 non-polymer

description	SULFATE ION
formula_weight	96.1 kDa
src_method	syn
entity_count	2

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6. Citations (4)

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length_a	208.150 Å
length_b	167.700 Å
length_c	108.190 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	24

space_group	P 21 21 2
int_tables_number	18
crystal_system	—

resolution	3.000 Å
resolution_low	25.000 Å
r_factor	0.2940
r_work	0.2940
r_free	0.3420

fit	/app/data/saxs_computation/do/1do0/1do0.fit
pddf	/app/data/pr_computation/do/1do0/1do0.pr.out
profile	/app/data/saxs_computation/do/1do0/1do0.dat

ORTHORHOMBIC CRYSTAL FORM OF HEAT SHOCK LOCUS U (HSLU) FROM ESCHERICHIA COLI

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction