STRUCTURE OF THE H61T MUTANT OF THE FLAVOENZYME VANILLYL-ALCOHOL OXIDASE IN THE APO FORM

Method: X-RAY DIFFRACTION D_max: 94.6 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1e8f
deposition_date	2000-09-20
title	STRUCTURE OF THE H61T MUTANT OF THE FLAVOENZYME VANILLYL-ALCOHOL OXIDASE IN THE APO FORM
keywords	OXIDOREDUCTASE, FLAVOPROTEIN, METHANOL UTILIZATION, PEROXISOME, FLAVOENZYME, OXIDASE, CATALYSIS; OXIDOREDUCTASE
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	30.36 Å
rg_electron	29.44 Å
i0	224467000.00
molecular_weight	122300.0 kDa
excluded_volume	153960 Å³
envelope_volume	177870 Å³
shell_volume	47978 Å³
envelope_diameter	100.8 Å
shell_rg	38.62 Å
envelope_rg	29.68 Å
shape_rg	29.43 Å
total_rg	30.23 Å
total_atoms	8622
n_residues	1090
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	94.6 Å
rg_real	30.21 Å
rg_real_error	0.57 Å
i0_real	2.2450e+08
i0_real_error	3.1140e+06
rg_reciprocal	30.28 Å
i0_reciprocal	224500000.0000
total_estimate	0.8910
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	38.2 Å
skewness	0.214
kurtosis	-0.393
angular_range	— – 0.2600 Å⁻¹
current_alpha	0.0000
highest_alpha	96590000.0000
n_real_points	53
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.906; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.988; Smooth: 0.871

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

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6. Citations (1)

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length_a	129.280 Å
length_b	129.280 Å
length_c	130.400 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	I 4
int_tables_number	79
crystal_system	—

resolution	2.900 Å
resolution_low	20.000 Å
r_factor	0.2390
r_work	0.2390
r_free	0.3120

description	VANILLYL-ALCOHOL OXIDASE
formula_weight	62969.9 kDa
ec_number	1.1.3.7
mutation	YES
src_method	man
entity_count	2

type	polypeptide(L)
strand_id	A,B
atom_count	8642

STRUCTURE OF THE H61T MUTANT OF THE FLAVOENZYME VANILLYL-ALCOHOL OXIDASE IN THE APO FORM

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	PENICILLIUM SIMPLICISSIMUM
ncbi_taxonomy_id	69488
host_organism	ESCHERICHIA COLI

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	20

fit	/app/data/saxs_computation/e8/1e8f/1e8f.fit
pddf	/app/data/pr_computation/e8/1e8f/1e8f.pr.out
profile	/app/data/saxs_computation/e8/1e8f/1e8f.dat