← Back to search

1e8h

STRUCTURE OF THE H61T MUTANT OF THE FLAVOENZYME VANILLYL-ALCOHOL OXIDASE IN THE APO FORM COMPLEXED BY ADP

Method: X-RAY DIFFRACTION D_max: 94.4 Å Quality: GOOD

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	1e8h
deposition_date	2000-09-20
title	STRUCTURE OF THE H61T MUTANT OF THE FLAVOENZYME VANILLYL-ALCOHOL OXIDASE IN THE APO FORM COMPLEXED BY ADP
keywords	OXIDOREDUCTASE, FLAVOPROTEIN, METHANOL UTILIZATION, PEROXISOME, FLAVOENZYME, OXIDASE, CATALYSIS; OXIDOREDUCTASE
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	30.45 Å
rg_electron	29.58 Å
i0	231047000.00
molecular_weight	123150.0 kDa
excluded_volume	154680 Å³
envelope_volume	181320 Å³
shell_volume	48588 Å³
envelope_diameter	99.9 Å
shell_rg	38.78 Å
envelope_rg	29.83 Å
shape_rg	29.57 Å
total_rg	30.36 Å
total_atoms	8676
n_residues	1090
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	94.4 Å
rg_real	30.30 Å
rg_real_error	0.49 Å
i0_real	2.3100e+08
i0_real_error	3.3830e+06
rg_reciprocal	30.37 Å
i0_reciprocal	231100000.0000
total_estimate	0.8970
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	38.1 Å
skewness	0.215
kurtosis	-0.396
angular_range	— – 0.2600 Å⁻¹
current_alpha	0.0000
highest_alpha	115700000.0000
n_real_points	53
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.913; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.988; Smooth: 0.929

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (3)

Entity 1 polymer

description	VANILLYL-ALCOHOL OXIDASE
formula_weight	62969.9 kDa
ec_number	1.1.3.7
mutation	YES
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	8765

MSKTQEFRPLTLPPKLSLSDFNEFIQDIIRIVGSENVEVISSKDQIVDGSYMKPTHTHDPTHVMDQDYFLASAIVAPRNVADVQSIVGLANKFSFPLWPISIGRNSGYGGAAPRVSGSVVLDMGKNMNRVLEVNVEGAYCVVEPGVTYHDLHNYLEANNLRDKLWLDVPDLGGGSVLGNAVERGVGYTPYGDHWMMHSGMEVVLANGELLRTGMGALPDPKRPETMGLKPEDQPWSKIAHLFPYGFGPYIDGLFSQSNMGIVTKIGIWLMPNPGGYQSYLITLPKDGDLKQAVDIIRPLRLGMALQNVPTIRHILLDAAVLGDKRSYSSRTEPLSDEELDKIAKQLNLGRWNFYGALYGPEPIRRVLWETIKDAFSAIPGVKFYFPEDTPENSVLRVRDKTMQGIPTYDELKWIDWLPNGAHLFFSPIAKVSGEDAMMQYAVTKKRCQEAGLDFIGTFTVGMREMHHIVCIVFNKKDLIQKRKVQWLMRTLIDDCAANGWGEYRTHLAFMDQIMETYNWNNSSFLRFNEVLKNAVDPNGIIAPGKSGVWPSQYSHVTWKL

Source

organism	PENICILLIUM SIMPLICISSIMUM
ncbi_taxonomy_id	69488
host_organism	ESCHERICHIA COLI

Entity 2 non-polymer

description	ADENOSINE-5'-DIPHOSPHATE
formula_weight	427.2 kDa
src_method	syn
entity_count	2

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	89

▶

6. Citations (1)

▶

length_a	130.340 Å
length_b	130.340 Å
length_c	134.000 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	I 4
int_tables_number	79
crystal_system	—

resolution	2.600 Å
resolution_low	20.000 Å
r_factor	0.2370
r_work	0.2370
r_free	0.3030

fit	/app/data/saxs_computation/e8/1e8h/1e8h.fit
pddf	/app/data/pr_computation/e8/1e8h/1e8h.pr.out
profile	/app/data/saxs_computation/e8/1e8h/1e8h.dat

STRUCTURE OF THE H61T MUTANT OF THE FLAVOENZYME VANILLYL-ALCOHOL OXIDASE IN THE APO FORM COMPLEXED BY ADP

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction