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1ecf

ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE

Method: X-RAY DIFFRACTION D_max: 96.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1ecf
deposition_date	1996-04-23
title	ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE
keywords	PURINE BIOSYNTHESIS, TRANSFERASE, GLYCOSYLTRANSFERASE, GLUTAMINE AMIDOTRANSFERASE, TRANSFERASE (GLUTAMINE AMIDOTRANSFERASE); TRANSFERASE (GLUTAMINE AMIDOTRANSFERASE)
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	31.01 Å
rg_electron	30.07 Å
i0	207511000.00
molecular_weight	112130.0 kDa
excluded_volume	139300 Å³
envelope_volume	170090 Å³
shell_volume	45785 Å³
envelope_diameter	101.9 Å
shell_rg	38.30 Å
envelope_rg	29.88 Å
shape_rg	30.10 Å
total_rg	30.64 Å
total_atoms	7874
n_residues	992
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	96.2 Å
rg_real	30.87 Å
rg_real_error	0.51 Å
i0_real	2.0750e+08
i0_real_error	3.0050e+06
rg_reciprocal	30.93 Å
i0_reciprocal	207500000.0000
total_estimate	0.8985
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	37.7 Å
skewness	0.222
kurtosis	-0.436
angular_range	— – 0.2550 Å⁻¹
current_alpha	0.0000
highest_alpha	68490000.0000
n_real_points	52
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.932; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.996; Smooth: 0.883

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE AMIDOTRANSFERASE
formula_weight	56422.7 kDa
ec_number	2.4.2.14
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B
atom_count	9124

CGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFRLRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHNGNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAATNRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLGFDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSARVNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRTFIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVYLASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAENPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG

Source

organism	Escherichia coli
ncbi_taxonomy_id	562
host_organism	Escherichia coli BL21(DE3)

Entity 2 non-polymer

description	;PIPERAZINE-N,N'-BIS(2-ETHANESULFONIC ACID) ;
formula_weight	302.4 kDa
src_method	syn
entity_count	6

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	986

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6. Citations (1)

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length_a	116.900 Å
length_b	157.500 Å
length_c	106.300 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	C 2 2 21
int_tables_number	20
crystal_system	—

resolution	2.000 Å
resolution_low	30.000 Å
r_factor	0.1750
r_work	0.1750
r_free	0.2300

fit	/app/data/saxs_computation/ec/1ecf/1ecf.fit
pddf	/app/data/pr_computation/ec/1ecf/1ecf.pr.out
profile	/app/data/saxs_computation/ec/1ecf/1ecf.dat

ESCHERICHIA COLI GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction