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1egw

CRYSTAL STRUCTURE OF MEF2A CORE BOUND TO DNA

Method: X-RAY DIFFRACTION D_max: 89.2 Å Quality: EXCELLENT

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1egw
deposition_date	2000-02-17
title	CRYSTAL STRUCTURE OF MEF2A CORE BOUND TO DNA
keywords	MADS-box transcription factor, DNA-protein complex, transcription/DNA, TRANSCRIPTION-DNA complex; TRANSCRIPTION/DNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	28.06 Å
rg_electron	27.26 Å
i0	70050300.00
molecular_weight	53671.0 kDa
excluded_volume	61997 Å³
envelope_volume	82547 Å³
shell_volume	25960 Å³
envelope_diameter	95.3 Å
shell_rg	33.49 Å
envelope_rg	27.03 Å
shape_rg	27.25 Å
total_rg	27.84 Å
total_atoms	3682
n_residues	354
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	89.2 Å
rg_real	28.13 Å
rg_real_error	0.66 Å
i0_real	7.0050e+07
i0_real_error	1.0340e+06
rg_reciprocal	28.11 Å
i0_reciprocal	70050000.0000
total_estimate	0.9007
solution_quality	EXCELLENT a EXCELLENT solution
n_peaks	2
r_peak_primary	31.3 Å
skewness	0.332
kurtosis	-0.505
angular_range	— – 0.2850 Å⁻¹
current_alpha	0.0000
highest_alpha	2928000.0000
n_real_points	58
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.947; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.965; Smooth: 0.900

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4. Crystallography & Experiment

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5. Entities & Polymer Info (4)

Entity 1 polymer

description	;DNA (5'-D(APAPAPGPCPTPAPTPTPAPTPTPAPGPCPTP*T)-3') ;
formula_weight	5200.4 kDa
src_method	syn
entity_count	2

Polymer

type	polydeoxyribonucleotide
strand_id	E,H
atom_count	5926

AAAGCTATTATTAGCTT

Entity 2 polymer

description	;DNA (5'-D(TPAPAPGPCPTPAPAPTPAPAPTPAPGPCPTP*T)-3') ;
formula_weight	5209.4 kDa
src_method	syn
entity_count	2

Polymer

type	polydeoxyribonucleotide
strand_id	F,G

TAAGCTAATAATAGCTT

Entity 3 polymer

description	MADS BOX TRANSCRIPTION ENHANCER FACTOR 2, POLYPEPTIDE A
formula_weight	9155.6 kDa
src_method	man
entity_count	4

Polymer

type	polypeptide(L)
strand_id	A,B,C,D

GRKKIQITRIMDERNRQVTFTKRKFGLMKKAYELSVLCDCEIALIIFNSSNKLFQYASTDMDKVLLKYTEYNEPHES

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	656

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6. Citations (1)

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length_a	41.371 Å
length_b	60.696 Å
length_c	63.987 Å
angle_alpha	115.18 °
angle_beta	89.99 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 1
int_tables_number	1
crystal_system	—

resolution	1.500 Å
resolution_low	20.000 Å
r_factor	—
r_work	0.2060
r_free	0.2290

fit	/app/data/saxs_computation/eg/1egw/1egw.fit
pddf	/app/data/pr_computation/eg/1egw/1egw.pr.out
profile	/app/data/saxs_computation/eg/1egw/1egw.dat

CRYSTAL STRUCTURE OF MEF2A CORE BOUND TO DNA

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

Source

Entity 4 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)