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1eiy

THE CRYSTAL STRUCTURE OF PHENYLALANYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED WITH COGNATE TRNAPHE

Method: X-RAY DIFFRACTION D_max: 166.6 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1eiy
deposition_date	2000-02-29
title	THE CRYSTAL STRUCTURE OF PHENYLALANYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED WITH COGNATE TRNAPHE
keywords	aminoacyl-tRNA synthetase, tRNA recognition, LIGASE-RNA COMPLEX; LIGASE/RNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	42.58 Å
rg_electron	43.55 Å
i0	407406000.00
molecular_weight	149850.0 kDa
excluded_volume	181270 Å³
envelope_volume	278430 Å³
shell_volume	56223 Å³
envelope_diameter	176.9 Å
shell_rg	44.85 Å
envelope_rg	45.53 Å
shape_rg	43.50 Å
total_rg	43.74 Å
total_atoms	10485
n_residues	1206
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	166.6 Å
rg_real	43.01 Å
rg_real_error	2.26 Å
i0_real	4.0740e+08
i0_real_error	8.4130e+06
rg_reciprocal	42.58 Å
i0_reciprocal	407200000.0000
total_estimate	0.7957
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	49.5 Å
skewness	0.733
kurtosis	0.594
angular_range	— – 0.1850 Å⁻¹
current_alpha	0.0000
highest_alpha	27420000.0000
n_real_points	38
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.528; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.806; Smooth: 0.950

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	TRNA(PHE)
formula_weight	24484.6 kDa
src_method	nat
entity_count	1

Polymer

type	polyribonucleotide
strand_id	C
atom_count	10485

GCCGAGGUAGCUCAGUUGGUAGAGCAUGCGACUGAAAAUCGCAGUGUCCGCGGUUCGAUUCCGCGCCUCGGCACCA

Entity 2 polymer

description	PHENYLALANYL-TRNA SYNTHETASE
formula_weight	39309.2 kDa
ec_number	6.1.1.20
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A

MLEEALAAIQNARDLEELKALKARYLGKKGLLTQEMKGLSALPLEERRKRGQELNAIKAALEAALEAREKALEEAALKEALERERVDVSLPGASLFSGGLHPITLMERELVEIFRALGYQAVEGPEVESEFFNFDALNIPEHHPARDMWDTFWLTGEGFRLEGPLGEEVEGRLLLRTHTSPMQVRYMVAHTPPFRIVVPGRVFRFEQTDATHEAVFHQLEGLVVGEGIAMAHLKGAIYELAQALFGPDSKVRFQPVYFPFVEPGAQFAVWWPEGGKWLELGGAGMVHPKVFQAVDAYRERLGLPPAYRGVTGFAFGLGVERLAMLRYGIPDIRYFFGGRLKFLEQFKGVL

Entity 3 polymer

description	PHENYLALANYL-TRNA SYNTHETASE
formula_weight	86720.7 kDa
ec_number	6.1.1.20
src_method	nat
entity_count	1

Polymer

type	polypeptide(L)
strand_id	B

MRVPFSWLKAYVPELESPEVLEERLAGLGFETDRIERVFPIPRGVVFARVLEAHPIPGTRLKRLVLDAGRTVEVVSGAENARKGIGVALALPGTELPGLGQKVGERVIQGVRSFGMALSPRELGVGEYGGGLLEFPEDALPPGTPLSEAWPEEVVLDLEVTPNRPDALGLLGLARDLHALGYALVEPEAALKAEALPLPFALKVEDPEGAPHFTLGYAFGLRVAPSPLWMQRALFAAGMRPINNVVDVTNYVMLERAQPMHAFDLRFVGEGIAVRRAREGERLKTLDGVERTLHPEDLVIAGWRGEESFPLGLAGVMGGAESEVREDTEAIALEVACFDPVSIRKTARRHGLRTEASHRFERGVDPLGQVPAQRRALSLLQALAGARVAEALLEAGSPKPPEAIPFRPEYANRLLGTSYPEAEQIAILKRLGCRVEGEGPTYRVTPPSHRLDLRLEEDLVEEVARIQGYETIPLALPAFFPAPDNRGVEAPYRKEQRLREVLSGLGFQEVYTYSFMDPEDARRFRLDPPRLLLLNPLAPEKAALRTHLFPGLVRVLKENLDLDRPERALLFEVGRVFREREETHLAGLLFGEGVGLPWAKERLSGYFLLKGYLEALFARLGLAFRVEAQAFPFLHPGVSGRVLVEGEEVGFLGALHPEIAQELELPPVHLFELRLPLPDKPLAFQDPSRHPAAFRDLAVVVPAPTPYGEVEALVREAAGPYLESLALFDLYQGPPLPEGHKSLAFHLRFRHPKRTLRDEEVEEAVSRVAEALRARGFGLRGLDTP

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6. Citations (1)

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length_a	175.000 Å
length_b	175.000 Å
length_c	140.600 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	6

space_group	P 32 2 1
int_tables_number	154
crystal_system	—

resolution	3.300 Å
resolution_low	28.000 Å
r_factor	—
r_work	0.2210
r_free	0.2870

fit	/app/data/saxs_computation/ei/1eiy/1eiy.fit
pddf	/app/data/pr_computation/ei/1eiy/1eiy.pr.out
profile	/app/data/saxs_computation/ei/1eiy/1eiy.dat

THE CRYSTAL STRUCTURE OF PHENYLALANYL-TRNA SYNTHETASE FROM THERMUS THERMOPHILUS COMPLEXED WITH COGNATE TRNAPHE

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Entity 3 polymer

Polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)