2.1A X-RAY STRUCTURE OF TOLC: AN INTEGRAL OUTER MEMBRANE PROTEIN AND EFFLUX PUMP COMPONENT FROM ESCHERICHIA COLI

Method: X-RAY DIFFRACTION D_max: 149.7 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1ek9
deposition_date	2000-03-07
title	2.1A X-RAY STRUCTURE OF TOLC: AN INTEGRAL OUTER MEMBRANE PROTEIN AND EFFLUX PUMP COMPONENT FROM ESCHERICHIA COLI
keywords	Integral membrane protein, Alpha helical Barrel, Beta Barrel, MEMBRANE PROTEIN; MEMBRANE PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	41.14 Å
rg_electron	41.04 Å
i0	337129000.00
molecular_weight	141600.0 kDa
excluded_volume	174410 Å³
envelope_volume	258420 Å³
shell_volume	57331 Å³
envelope_diameter	148.6 Å
shell_rg	42.76 Å
envelope_rg	39.87 Å
shape_rg	41.02 Å
total_rg	41.21 Å
total_atoms	9918
n_residues	1269
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	149.7 Å
rg_real	41.48 Å
rg_real_error	1.74 Å
i0_real	3.3710e+08
i0_real_error	5.8330e+06
rg_reciprocal	41.15 Å
i0_reciprocal	337000000.0000
total_estimate	0.7825
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	43.4 Å
skewness	0.677
kurtosis	0.097
angular_range	— – 0.1900 Å⁻¹
current_alpha	0.0000
highest_alpha	33730000.0000
n_real_points	39
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.477; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.969; Smooth: 0.770

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

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6. Citations (2)

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length_a	265.049 Å
length_b	265.049 Å
length_c	95.959 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	120.00 °
z_pdb	27

space_group	H 3
int_tables_number	146
crystal_system	—

resolution	2.100 Å
resolution_low	20.000 Å
r_factor	0.2080
r_work	0.2080
r_free	0.2570

description	OUTER MEMBRANE PROTEIN TOLC
formula_weight	47238.3 kDa
src_method	man
entity_count	3

type	polypeptide(L)
strand_id	A,B,C
atom_count	11426

2.1A X-RAY STRUCTURE OF TOLC: AN INTEGRAL OUTER MEMBRANE PROTEIN AND EFFLUX PUMP COMPONENT FROM ESCHERICHIA COLI

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 water

6. Citations (2)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Escherichia coli
ncbi_taxonomy_id	562
host_organism	Escherichia coli

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	1508

fit	/app/data/saxs_computation/ek/1ek9/1ek9.fit
pddf	/app/data/pr_computation/ek/1ek9/1ek9.pr.out
profile	/app/data/saxs_computation/ek/1ek9/1ek9.dat