TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 E35K

Method: X-RAY DIFFRACTION D_max: 39.4 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1ekl
deposition_date	1999-01-21
title	TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 E35K
keywords	ANTIFREEZE PROTEIN, MUTANT, ICE BINDING PROTEIN, THERMAL HYSTERESIS PROTEIN; ANTIFREEZE PROTEIN
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	11.76 Å
rg_electron	10.52 Å
i0	1018410.00
molecular_weight	6932.0 kDa
excluded_volume	8837 Å³
envelope_volume	9098 Å³
shell_volume	7676 Å³
envelope_diameter	38.1 Å
shell_rg	15.82 Å
envelope_rg	10.81 Å
shape_rg	10.52 Å
total_rg	11.99 Å
total_atoms	482
n_residues	66
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	39.4 Å
rg_real	11.68 Å
rg_real_error	0.28 Å
i0_real	1.0180e+06
i0_real_error	1.1120e+04
rg_reciprocal	11.68 Å
i0_reciprocal	1018000.0000
total_estimate	0.8554
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	15.5 Å
skewness	0.166
kurtosis	-0.150
angular_range	— – 0.5000 Å⁻¹
current_alpha	0.0000
highest_alpha	236600.0000
n_real_points	80
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.728; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.999; Smooth: 0.932

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4. Crystallography & Experiment

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5. Entities & Polymer Info (2)

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6. Citations (6)

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length_a	32.544 Å
length_b	38.893 Å
length_c	46.615 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	1.650 Å
resolution_low	8.000 Å
r_factor	—
r_work	0.2100
r_free	0.2230

description	PROTEIN (ANTIFREEZE PROTEIN TYPE III)
formula_weight	6979.3 kDa
mutation	E35K, P64A, P65A
src_method	man
entity_count	1

type	polypeptide(L)
strand_id	A
atom_count	543

TYPE III ANTIFREEZE PROTEIN ISOFORM HPLC 12 E35K

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (2)

Entity 1 polymer

Polymer

Source

Entity 2 water

6. Citations (6)

7. Files & Curves (10)

Files (7)

Curves (3)

organism	Macrozoarces americanus
common_name	ocean pout
ncbi_taxonomy_id	8199
host_organism	Escherichia coli

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	61

fit	/app/data/saxs_computation/ek/1ekl/1ekl.fit
pddf	/app/data/pr_computation/ek/1ekl/1ekl.pr.out
profile	/app/data/saxs_computation/ek/1ekl/1ekl.dat