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1eop

ECORV BOUND TO COGNATE DNA

Method: X-RAY DIFFRACTION D_max: 76.3 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1eop
deposition_date	2000-03-23
title	ECORV BOUND TO COGNATE DNA
keywords	protein-DNA recognition, induced fit, endonuclease, hydrolase-DNA COMPLEX; hydrolase/DNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	23.96 Å
rg_electron	23.32 Å
i0	66316300.00
molecular_weight	60821.0 kDa
excluded_volume	74736 Å³
envelope_volume	87695 Å³
shell_volume	30555 Å³
envelope_diameter	77.8 Å
shell_rg	31.12 Å
envelope_rg	23.44 Å
shape_rg	23.33 Å
total_rg	24.07 Å
total_atoms	4289
n_residues	501
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	76.3 Å
rg_real	23.83 Å
rg_real_error	0.38 Å
i0_real	6.6320e+07
i0_real_error	8.0320e+05
rg_reciprocal	23.86 Å
i0_reciprocal	66320000.0000
total_estimate	0.8910
solution_quality	GOOD a GOOD solution
n_peaks	1
r_peak_primary	29.8 Å
skewness	0.243
kurtosis	-0.338
angular_range	— – 0.3300 Å⁻¹
current_alpha	0.0000
highest_alpha	16770000.0000
n_real_points	65
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.865; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.996; Smooth: 0.987

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	;DNA (5'-D(GPAPAPGPAPTPAPTPCPTPTPC)-3') ;
formula_weight	3685.4 kDa
src_method	syn
entity_count	2

Polymer

type	polydeoxyribonucleotide
strand_id	C,D
atom_count	4359

GAAGATATCTTA

Entity 2 polymer

description	TYPE II RESTRICTION ENZYME ECORV
formula_weight	28690.4 kDa
ec_number	3.1.21.4
src_method	man
entity_count	2

Polymer

type	polypeptide(L)
strand_id	A,B

MSLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPIINKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENEKIKFTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVATRKSSLKTYNINELNEIPKPYKGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKGIFDSEDEFLDYWRNYERTSQLRNDKYNNISEYRNWIYRGRK

Source

organism	Escherichia coli
ncbi_taxonomy_id	562
host_organism	Escherichia coli

Entity 3 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	70

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6. Citations (1)

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length_a	66.100 Å
length_b	66.100 Å
length_c	299.100 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	16

space_group	P 41 21 2
int_tables_number	92
crystal_system	—

resolution	2.600 Å
resolution_low	4.800 Å
r_factor	0.1870
r_work	0.1810
r_free	0.3020

fit	/app/data/saxs_computation/eo/1eop/1eop.fit
pddf	/app/data/pr_computation/eo/1eop/1eop.pr.out
profile	/app/data/saxs_computation/eo/1eop/1eop.dat

ECORV BOUND TO COGNATE DNA

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Source

Entity 3 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)