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1euq

CRYSTAL STRUCTURE OF GLUTAMINYL-TRNA SYNTHETASE COMPLEXED WITH A TRNA-GLN MUTANT AND AN ACTIVE-SITE INHIBITOR

Method: X-RAY DIFFRACTION D_max: 101.5 Å Quality: GOOD

SAXS Profile

P(r) Distribution

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1. Structure Basics

entry_id	1euq
deposition_date	2000-04-17
title	CRYSTAL STRUCTURE OF GLUTAMINYL-TRNA SYNTHETASE COMPLEXED WITH A TRNA-GLN MUTANT AND AN ACTIVE-SITE INHIBITOR
keywords	TRNA SYNTHETASE, GLUTAMINE, TRNAGLN, E. COLI, RNA-PROTEIN COMPLEX, ligase-RNA COMPLEX; ligase/RNA
method	X-RAY DIFFRACTION

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Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

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2. SAXS Parameters (CRYSOL)

rg_guinier	30.55 Å
rg_electron	30.66 Å
i0	165030000.00
molecular_weight	84434.0 kDa
excluded_volume	97791 Å³
envelope_volume	124180 Å³
shell_volume	35176 Å³
envelope_diameter	108.3 Å
shell_rg	36.31 Å
envelope_rg	30.63 Å
shape_rg	30.65 Å
total_rg	31.06 Å
total_atoms	5844
n_residues	601
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

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3. P(r) Analysis (GNOM)

dmax	101.5 Å
rg_real	30.61 Å
rg_real_error	0.78 Å
i0_real	1.6500e+08
i0_real_error	2.5270e+06
rg_reciprocal	30.59 Å
i0_reciprocal	165000000.0000
total_estimate	0.8900
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	33.2 Å
skewness	0.388
kurtosis	-0.351
angular_range	— – 0.2600 Å⁻¹
current_alpha	0.0000
highest_alpha	13860000.0000
n_real_points	53
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.885; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.959; Smooth: 0.952

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4. Crystallography & Experiment

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5. Entities & Polymer Info (3)

Entity 1 polymer

description	GLUTAMINYL TRNA
formula_weight	23181.8 kDa
src_method	syn
entity_count	1

Polymer

type	polyribonucleotide
strand_id	B
atom_count	5844

GGGGUAUCGCCAAGCGGUAAGGCACCGGAUUCUGAUUCCGGCAGCGAGGUUCGAAUCCUCGUACCCCAGCCA

Entity 2 polymer

description	GLUTAMINYL-TRNA SYNTHETASE
formula_weight	62894.1 kDa
ec_number	6.1.1.18
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A

MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSDYFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEKMRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNLLVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIREDLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRADFREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADGRKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSLKDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDT

Source

organism	Escherichia coli
ncbi_taxonomy_id	562
host_organism	Escherichia coli

Entity 3 non-polymer

description	5'-O-[N-(L-GLUTAMINYL)-SULFAMOYL]ADENOSINE
formula_weight	474.4 kDa
src_method	syn
entity_count	1

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6. Citations (1)

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length_a	230.910 Å
length_b	93.590 Å
length_c	113.110 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	C 2 2 21
int_tables_number	20
crystal_system	—

resolution	3.100 Å
resolution_low	30.000 Å
r_factor	—
r_work	0.2420
r_free	0.2850

fit	/app/data/saxs_computation/eu/1euq/1euq.fit
pddf	/app/data/pr_computation/eu/1euq/1euq.pr.out
profile	/app/data/saxs_computation/eu/1euq/1euq.dat

CRYSTAL STRUCTURE OF GLUTAMINYL-TRNA SYNTHETASE COMPLEXED WITH A TRNA-GLN MUTANT AND AN ACTIVE-SITE INHIBITOR

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (3)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)