← Back to search

1euy

GLUTAMINYL-TRNA SYNTHETASE COMPLEXED WITH A TRNA MUTANT AND AN ACTIVE SITE INHIBITOR

Method: X-RAY DIFFRACTION D_max: 101.7 Å Quality: GOOD

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	1euy
deposition_date	2000-04-19
title	GLUTAMINYL-TRNA SYNTHETASE COMPLEXED WITH A TRNA MUTANT AND AN ACTIVE SITE INHIBITOR
keywords	TRNA SYNTHETASE, GLUTAMINE, TRNAGLN, E. COLI, COMPLEX, ligase-RNA COMPLEX; ligase/RNA
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	30.61 Å
rg_electron	30.74 Å
i0	166580000.00
molecular_weight	84692.0 kDa
excluded_volume	98004 Å³
envelope_volume	125520 Å³
shell_volume	35402 Å³
envelope_diameter	108.0 Å
shell_rg	36.40 Å
envelope_rg	30.74 Å
shape_rg	30.73 Å
total_rg	31.13 Å
total_atoms	5861
n_residues	602
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	101.7 Å
rg_real	30.67 Å
rg_real_error	0.69 Å
i0_real	1.6660e+08
i0_real_error	2.5610e+06
rg_reciprocal	30.65 Å
i0_reciprocal	166600000.0000
total_estimate	0.8901
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	33.2 Å
skewness	0.388
kurtosis	-0.349
angular_range	— – 0.2600 Å⁻¹
current_alpha	0.0000
highest_alpha	13770000.0000
n_real_points	53
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.886; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.959; Smooth: 0.950

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (4)

Entity 1 polymer

description	GLUTAMINYL TRNA
formula_weight	23817.2 kDa
src_method	syn
entity_count	1

Polymer

type	polyribonucleotide
strand_id	B
atom_count	5915

UGGGGUAUCGCCAAGCGGUAAGGCACCGGAUUCUGAUUCCGGCAAGCGAGGUUCGAAUCCUCGUACCCCAGCCA

Entity 2 polymer

description	GLUTAMINYL-TRNA SYNTHETASE
formula_weight	62894.1 kDa
ec_number	6.1.1.18
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A

MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGHAKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSDYFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEKMRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCISDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNLLVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIREDLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRADFREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADGRKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSLKDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDT

Source

organism	Escherichia coli
ncbi_taxonomy_id	562
host_organism	Escherichia coli

Entity 3 non-polymer

description	5'-O-[N-(L-GLUTAMINYL)-SULFAMOYL]ADENOSINE
formula_weight	474.4 kDa
src_method	syn
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	54

▶

6. Citations (1)

▶

length_a	235.200 Å
length_b	94.200 Å
length_c	113.410 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	8

space_group	C 2 2 21
int_tables_number	20
crystal_system	—

resolution	2.600 Å
resolution_low	30.000 Å
r_factor	—
r_work	0.2340
r_free	0.2770

fit	/app/data/saxs_computation/eu/1euy/1euy.fit
pddf	/app/data/pr_computation/eu/1euy/1euy.pr.out
profile	/app/data/saxs_computation/eu/1euy/1euy.dat

GLUTAMINYL-TRNA SYNTHETASE COMPLEXED WITH A TRNA MUTANT AND AN ACTIVE SITE INHIBITOR

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction

5. Entities & Polymer Info (4)

Entity 1 polymer

Polymer

Entity 2 polymer

Polymer

Source

Entity 3 non-polymer

Entity 4 water

6. Citations (1)

7. Files & Curves (10)

Files (7)

Curves (3)