← Back to search

1f20

CRYSTAL STRUCTURE OF RAT NEURONAL NITRIC-OXIDE SYNTHASE FAD/NADP+ DOMAIN AT 1.9A RESOLUTION.

Method: X-RAY DIFFRACTION D_max: 84.2 Å Quality: GOOD

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	1f20
deposition_date	2000-05-22
title	CRYSTAL STRUCTURE OF RAT NEURONAL NITRIC-OXIDE SYNTHASE FAD/NADP+ DOMAIN AT 1.9A RESOLUTION.
keywords	Nitric-xoide synthase, reductase domain, FAD, NADP+, OXIDOREDUCTASE; OXIDOREDUCTASE
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	26.33 Å
rg_electron	25.66 Å
i0	46911600.00
molecular_weight	51796.0 kDa
excluded_volume	64285 Å³
envelope_volume	80169 Å³
shell_volume	26414 Å³
envelope_diameter	88.6 Å
shell_rg	32.51 Å
envelope_rg	25.95 Å
shape_rg	25.64 Å
total_rg	26.46 Å
total_atoms	3641
n_residues	435
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	84.2 Å
rg_real	26.37 Å
rg_real_error	0.65 Å
i0_real	4.6910e+07
i0_real_error	6.1770e+05
rg_reciprocal	26.36 Å
i0_reciprocal	46910000.0000
total_estimate	0.8997
solution_quality	GOOD a GOOD solution
n_peaks	2
r_peak_primary	26.6 Å
skewness	0.364
kurtosis	-0.470
angular_range	— – 0.3000 Å⁻¹
current_alpha	0.0000
highest_alpha	7826000.0000
n_real_points	61
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.918; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.967; Smooth: 0.970

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (6)

Entity 1 polymer

description	NITRIC-OXIDE SYNTHASE
formula_weight	49732.5 kDa
ec_number	1.14.13.39
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	4120

SWKRNKFRLTYVAEAPDLTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGNQELQYQPGDHLGVFPGNHEDLVNALIERLEDAPPANHVVKVEMLEERNTALGVISNWKDESRLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATNEKEKQRLLVLSKGLQEYEEWKWGKNPTMVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYHTRDGEGPVHHGVCSSWLNRIQADDVVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFRSFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSREPDRPKKYVQDVLQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLSEEDAGVFISRLRDDNRYHEDIFGV

Source

organism	Rattus norvegicus
common_name	Norway rat
ncbi_taxonomy_id	10116
host_organism	Escherichia coli

Entity 2 non-polymer

description	FLAVIN-ADENINE DINUCLEOTIDE
formula_weight	785.5 kDa
src_method	syn
entity_count	1

Entity 3 non-polymer

description	NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE
formula_weight	743.4 kDa
src_method	syn
entity_count	1

Entity 4 non-polymer

description	GLYCEROL
formula_weight	92.1 kDa
src_method	syn
entity_count	5

Entity 5 non-polymer

description	FORMIC ACID
formula_weight	46.0 kDa
src_method	syn
entity_count	3

Entity 6 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	479

▶

6. Citations (1)

▶

length_a	59.070 Å
length_b	64.370 Å
length_c	159.430 Å
angle_alpha	90.00 °
angle_beta	90.00 °
angle_gamma	90.00 °
z_pdb	4

space_group	P 21 21 21
int_tables_number	19
crystal_system	—

resolution	1.900 Å
resolution_low	32.000 Å
r_factor	—
r_work	0.1862
r_free	0.2060

fit	/app/data/saxs_computation/f2/1f20/1f20.fit
pddf	/app/data/pr_computation/f2/1f20/1f20.pr.out
profile	/app/data/saxs_computation/f2/1f20/1f20.dat