← Back to search

1f5n

HUMAN GUANYLATE BINDING PROTEIN-1 IN COMPLEX WITH THE GTP ANALOGUE, GMPPNP.

Method: X-RAY DIFFRACTION D_max: 145.2 Å Quality: REASONABLE

SAXS Profile

P(r) Distribution

▼

1. Structure Basics

entry_id	1f5n
deposition_date	2000-06-15
title	HUMAN GUANYLATE BINDING PROTEIN-1 IN COMPLEX WITH THE GTP ANALOGUE, GMPPNP.
keywords	GBP, GTP Hydrolysis, GDP, GMP, Interferon Induced, Dynamin related, Large GTPase family. GMPPNP, GPPNHP., SIGNALING PROTEIN; SIGNALING PROTEIN
method	X-RAY DIFFRACTION

▼

Download Data

SAXS curve (.dat) P(r) distribution (.pr.out) Structure (CIF)

▼

2. SAXS Parameters (CRYSOL)

rg_guinier	38.06 Å
rg_electron	39.29 Å
i0	68464300.00
molecular_weight	65866.0 kDa
excluded_volume	82397 Å³
envelope_volume	108280 Å³
shell_volume	26955 Å³
envelope_diameter	143.5 Å
shell_rg	37.46 Å
envelope_rg	40.02 Å
shape_rg	39.30 Å
total_rg	39.14 Å
total_atoms	4618
n_residues	570
n_harmonics	20
q_range	— – 0.5000 Å⁻¹
n_points	101
shell_type	directional
solvent_density	0.3340 e/Å³
contrast_shell	0.0300 e/Å³
crysol_version	4.1.3

▼

3. P(r) Analysis (GNOM)

dmax	145.2 Å
rg_real	39.19 Å
rg_real_error	2.12 Å
i0_real	6.8460e+07
i0_real_error	1.2900e+06
rg_reciprocal	38.49 Å
i0_reciprocal	68420000.0000
total_estimate	0.5866
solution_quality	REASONABLE a REASONABLE solution
n_peaks	1
r_peak_primary	27.7 Å
skewness	0.747
kurtosis	-0.297
angular_range	— – 0.2100 Å⁻¹
current_alpha	0.0000
highest_alpha	6878000.0000
n_real_points	43
gnom_version	4.1.3
quality_criteria	AN1: 0.000; Oscil: 0.174; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.098; Smooth: 0.000

▶

4. Crystallography & Experiment

▶

5. Entities & Polymer Info (4)

Entity 1 polymer

description	INTERFERON-INDUCED GUANYLATE-BINDING PROTEIN 1
formula_weight	68003.6 kDa
mutation	Q507H
src_method	man
entity_count	1

Polymer

type	polypeptide(L)
strand_id	A
atom_count	5029

MASEIHMTGPMCLIENTNGRLMANPEALKILSAITQPMVVVAIVGLYRTGKSYLMNKLAGKKKGFSLGSTVQSHTKGIWMWCVPHPKKPGHILVLLDTEGLGDVEKGDNQNDSWIFALAVLLSSTFVYNSIGTINQQAMDQLYYVTELTHRIRSKSSPDENENEVEDSADFVSFFPDFVWTLRDFSLDLEADGQPLTPDEYLTYSLKLKKGTSQKDETFNLPRLCIRKFFPKKKCFVFDRPVHRRKLAQLEKLQDEELDPEFVQQVADFCSYIFSNSKTKTLSGGIQVNGPRLESLVLTYVNAISSGDLPCMENAVLALAQIENSAAVQKAIAHYEQQMGQKVQLPTESLQELLDLHRDSEREAIEVFIRSSFKDVDHLFQKELAAQLEKKRDDFCKQNQEASSDRCSGLLQVIFSPLEEEVKAGIYSKPGGYRLFVQKLQDLKKKYYEEPRKGIQAEEILQTYLKSKESMTDAILQTDQTLTEKEKEIEVERVKAESAQASAKMLHEMQRKNEQMMEQKERSYQEHLKQLTEKMENDRVQLLKEQERTLALKLQEQEQLLKEGFQKESRIMKNEIQDLQTKMRRRKACTIS

Source

organism	Homo sapiens
common_name	human
ncbi_taxonomy_id	9606
host_organism	Escherichia coli

Entity 2 non-polymer

description	MAGNESIUM ION
formula_weight	24.3 kDa
src_method	syn
entity_count	1

Entity 3 non-polymer

description	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER
formula_weight	522.2 kDa
src_method	syn
entity_count	1

Entity 4 water

description	water
formula_weight	18.0 kDa
src_method	nat
entity_count	411

▶

6. Citations (3)

▶

length_a	161.228 Å
length_b	42.652 Å
length_c	91.544 Å
angle_alpha	90.00 °
angle_beta	100.19 °
angle_gamma	90.00 °
z_pdb	4

space_group	C 1 2 1
int_tables_number	5
crystal_system	—

resolution	1.700 Å
resolution_low	41.190 Å
r_factor	0.2260
r_work	0.2260
r_free	0.2550

fit	/app/data/saxs_computation/f5/1f5n/1f5n.fit
pddf	/app/data/pr_computation/f5/1f5n/1f5n.pr.out
profile	/app/data/saxs_computation/f5/1f5n/1f5n.dat

HUMAN GUANYLATE BINDING PROTEIN-1 IN COMPLEX WITH THE GTP ANALOGUE, GMPPNP.

SAXS Profile

P(r) Distribution

1. Structure Basics

Download Data

2. SAXS Parameters (CRYSOL)

3. P(r) Analysis (GNOM)

4. Crystallography & Experiment

Unit Cell

Symmetry

Refinement

Diffraction