1g8x

STRUCTURE OF A GENETICALLY ENGINEERED MOLECULAR MOTOR

Method: X-RAY DIFFRACTION D_max: 210.4 Å Quality: REASONABLE

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1. 蛋白身份与相关结构 Protein Identity & Related Structures

MYOSIN II HEAVY CHAIN FUSED TO ALPHA-ACTININ 3

Dictyostelium discoideum

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	信息不足同源多聚体蛋白 × 2 PDB 声明：dimeric（2）与蛋白拷贝数一致	链 A； UniProt 266–503 链 B； UniProt 266–503	片段：MYOSIN II HEAVY CHAIN, MOTOR DOMAIN RESIDUES 1-761, AND ALPHA-ACTININ 3, REPEATS 1 AND 2 RESIDUES 765-1002 突变：ARG238GLU	MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, SITTING DROP；pH 7.2；280 K；12% PEGM 5000; 170 mM NaCl; 50 mM HEPES (NaOH) pH 7.2; 5 mM MgCl2; 5 mM DTT; 0.5 mM EGTA; and 2% 2-methyl-1,3-propanediol, at 280 K, VAPOR DIFFUSION, SITTING DROP	分辨率 2.80 Å R-free 0.290

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly， “相对当前条目”直接指出证据层面的不同；没有差异标签表示当前已读取字段一致。

其他 PDB	相对当前条目 1G8X	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
4PD3 Crystal Structure of Rigor-Like Human Nonmuscle Myosin-2B 提交 2014-04-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 信息不足单体；蛋白 × 1 PDB 声明：monomeric	链 A 265–502（238 aa）片段：UNP P35580 residues 1-782, UNP P05095 residues 265-502	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 EVAPORATION；pH 7.5；291.15 K；10% PEG-6000, 0.1 M Tris pH 7.5	分辨率 2.84 Å R-free 0.288
4PD3 Crystal Structure of Rigor-Like Human Nonmuscle Myosin-2B 提交 2014-04-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 信息不足单体；蛋白 × 1 PDB 声明：monomeric	链 B 265–502（238 aa）片段：UNP P35580 residues 1-782, UNP P05095 residues 265-502	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 EVAPORATION；pH 7.5；291.15 K；10% PEG-6000, 0.1 M Tris pH 7.5	分辨率 2.84 Å R-free 0.288
5I4E Crystal Structure of Human Nonmuscle Myosin 2C motor domain 提交 2016-02-11	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 信息不足单体；蛋白 × 1 PDB 声明：monomeric	链 A 265–489（225 aa）片段：UNP residues 265-489,UNP residues 265-489	未记录	AOV ADP ORTHOVANADATE × 1 MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；281.15 K；TRIS PH 8.2, PEG 5K MME, MPD, NACL.	分辨率 2.25 Å R-free 0.241
5JLH Cryo-EM structure of a human cytoplasmic actomyosin complex at near-atomic resolution 提交 2016-04-27	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验方法不同实验环境不同结构质量不同	Assembly 1 信息不足异源复合物；蛋白 × 11 PDB 声明：undecameric	链 F 265–502（238 aa）链 G 265–502（238 aa）	未记录	ADP ADENOSINE-5'-DIPHOSPHATE × 5 MG MAGNESIUM ION × 5	ELECTRON MICROSCOPY cryo-EM缓冲液 pH 7.5；5 mM Tris-HCl pH 7.5, 1 mM DTT, 100 mM KCl, and 2 mM MgCl2 cryo-EM玻璃化条件冷冻剂 ETHANE；Sample (2 uL of F-actin-tropomyosin solution) was applied to a glow-discharged holey carbon grid, incubated for 20 s and manually blotted from the backside for less than a second with filter paper. Afterwards 1.5 uL of myosin solution (3 uM without nucleotide) were added directly on the grid, incubated for 10 s and then manually blotted for 5 s from the backside with filter paper.	分辨率 3.90 Å
9IHL Crystal Structure of the Human Nonmuscle Myosin 2A Motor Domain 提交 2025-02-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 信息不足单体；蛋白 × 1 PDB 声明：monomeric	链 A 265–502（238 aa）	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；291 K；16% PEG3350, 240 mM Sodium thiocyanate	分辨率 2.02 Å R-free 0.259

共 4 个其他 PDB 条目、5 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	AACT_DICDI
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 765–1002； UniProt 266–503 作者链 B； PDB构建体 765–1002； UniProt 266–503

MYOSIN II HEAVY CHAIN FUSED TO ALPHA-ACTININ 3

Dictyostelium discoideum

UniProt P08799

当前结构中的状态

Assembly	聚集状态	构建体	突变与修饰	配体、离子与共同组分	实验方法与环境	结构质量
1	信息不足同源多聚体蛋白 × 2 PDB 声明：dimeric（2）与蛋白拷贝数一致	链 A； UniProt 1–761 链 B； UniProt 1–761	片段：MYOSIN II HEAVY CHAIN, MOTOR DOMAIN RESIDUES 1-761, AND ALPHA-ACTININ 3, REPEATS 1 AND 2 RESIDUES 765-1002 突变：ARG238GLU	MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2	X-RAY DIFFRACTION X-ray结晶条件：VAPOR DIFFUSION, SITTING DROP；pH 7.2；280 K；12% PEGM 5000; 170 mM NaCl; 50 mM HEPES (NaOH) pH 7.2; 5 mM MgCl2; 5 mM DTT; 0.5 mM EGTA; and 2% 2-methyl-1,3-propanediol, at 280 K, VAPOR DIFFUSION, SITTING DROP	分辨率 2.80 Å R-free 0.290

数据库中的同蛋白其他状态

其他 PDB	相对当前条目 1G8X	Assembly / 聚集状态	构建体	突变与修饰	配体、离子与非聚合物	实验方法与环境	结构质量
1D0X DICTYOSTELIUM MYOSIN S1DC (MOTOR DOMAIN FRAGMENT) COMPLEXED WITH M-NITROPHENYL AMINOETHYLDIPHOSPHATE BERYLLIUM TRIFLUORIDE. 提交 1999-09-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–761（761 aa）片段：MYOSIN MOTOR DOMAIN	突变：Q760P, R761N	MG MAGNESIUM ION × 1 MNQ M-NITROPHENYL AMINOETHYLDIPHOSPHATE BERYLLIUM TRIFLUORIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 MICROBATCH；pH 7；277 K；8.3 % PEG 8000 125 MM NACL 3 MM DTT, pH 7.0, MICROBATCH, temperature 4.0K	分辨率 2.00 Å
1D0Y DICTYOSTELIUM MYOSIN S1DC (MOTOR DOMAIN FRAGMENT) COMPLEXED WITH O-NITROPHENYL AMINOETHYLDIPHOSPHATE BERYLLIUM FLUORIDE. 提交 1999-09-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–761（761 aa）片段：S1DC MOTOR DOMAIN	突变：Q760P, R761N	MG MAGNESIUM ION × 1 ONP O-NITROPHENYL AMINOETHYLDIPHOSPHATE BERYLLIUM TRIFLUORIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 MICROBATCH；pH 7；277 K；8.3 % PEG 8000 125 MM NACL 3 MM DTT, pH 7.0, MICROBATCH, temperature 4.0K	分辨率 2.00 Å
1D0Z DICTYOSTELIUM MYOSIN S1DC (MOTOR DOMAIN FRAGMENT) COMPLEXED WITH P-NITROPHENYL AMINOETHYLDIPHOSPHATE BERYLLIUM TRIFLUORIDE. 提交 1999-09-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–761（761 aa）片段：S1DC MOTOR DOMAIN	突变：Q760P, R761N	MG MAGNESIUM ION × 1 PNQ P-NITROPHENYL AMINOETHYLDIPHOSPHATE BERYLLIUM TRIFLUORIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 MICROBATCH；pH 7；277 K；8.3 % PEG 8000 125 mM NaCl 3 mM DTT, pH 7.0, microbatch, temperature 4.0K	分辨率 2.00 Å
1D1A DICTYOSTELIUM MYOSIN S1DC (MOTOR DOMAIN FRAGMENT) COMPLEXED WITH O,P-DINITROPHENYL AMINOETHYLDIPHOSPHATE BERYLLIUM TRIFLUORIDE. 提交 1999-09-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–761（761 aa）片段：S1DC MOTOR DOMAIN	突变：Q760P, R761N	MG MAGNESIUM ION × 1 DAE O,P-DINITROPHENYL AMINOETHYLDIPHOSPHATE-BERYLLIUM TRIFLUORIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 MICROBATCH；pH 7；277 K；8.3 % PEG 8000 125 MM NACL 3 MM DTT, pH 7.0, MICROBATCH, temperature 4.0K	分辨率 2.00 Å
1D1B DICTYOSTELIUM MYOSIN S1DC (MOTOR DOMAIN FRAGMENT) COMPLEXED WITH O,P-DINITROPHENYL AMINOPROPYLDIPHOSPHATE BERYLLIUM TRIFLUORIDE. 提交 1999-09-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–761（761 aa）片段：S1DC MOTOR DOMAIN	突变：Q760P, R761N	MG MAGNESIUM ION × 1 DAQ O,P-DINITROPHENYL AMINOPROPYLDIPHOSPHATE BERYLLIUM TRIFLUORIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 MICROBATCH；pH 7；277 K；8.3 % PEG 8000 125 mM NaCl 3 mM DTT, pH 7.0, microbatch, temperature 4.0K	分辨率 2.00 Å
1D1C DICTYOSTELIUM MYOSIN S1DC (MOTOR DOMAIN FRAGMENT) COMPLEXED WITH N-METHYL-O-NITROPHENYL AMINOETHYLDIPHOSPHATE BERYLLIUM TRIFLUORIDE. 提交 1999-09-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–761（761 aa）片段：S1DC MOTOR DOMAIN	突变：Q760P, R761N	MG MAGNESIUM ION × 1 NMQ N-METHYL O-NITROPHENYL AMINOETHYLDIPHOSPHATE BERYLLIUM TRIFLUORIDE × 1	X-RAY DIFFRACTION X-ray结晶条件 MICROBATCH；pH 7；277 K；8.3 % PEG 8000 125 MM NACL 3 MM DTT, pH 7.0, MICROBATCH, temperature 4.0K	分辨率 2.30 Å
1FMV CRYSTAL STRUCTURE OF THE APO MOTOR DOMAIN OF DICTYOSTELLIUM MYOSIN II 提交 2000-08-18	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–759（759 aa）片段：MOTOR DOMAIN	未记录	CL CHLORIDE ION × 1	X-RAY DIFFRACTION X-ray结晶条件 MICROBATCH；pH 7.5；277 K；8% Peg 8000, 50 mM Hepes, 1 mM DTT, pH 7.5, microbatch, temperature 4K	分辨率 2.10 Å R-free 0.280
1FMW CRYSTAL STRUCTURE OF THE MGATP COMPLEX FOR THE MOTOR DOMAIN OF DICTYOSTELIUM MYOSIN II 提交 2000-08-18	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–759（759 aa）片段：MOTOR DOMAIN	未记录	MG MAGNESIUM ION × 1 ATP ADENOSINE-5'-TRIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 7.5；277 K；8% Peg 8000, 50 mM Hepes, 1 mM DTT, pH 7.5, microbatch, temperature 277K	分辨率 2.15 Å R-free 0.286
1JWY CRYSTAL STRUCTURE OF THE DYNAMIN A GTPASE DOMAIN COMPLEXED WITH GDP, DETERMINED AS MYOSIN FUSION 提交 2001-09-05	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 信息不足单体；蛋白 × 1 PDB 声明：monomeric	链 A 3–765（763 aa）片段：catalytic domain	未记录	BGC beta-D-glucopyranose × 1 MG MAGNESIUM ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 1 GDP GUANOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 8.5；277 K；PEG 8000, Tris, potassium chloride, magnesium chloride, glucose, methyl-propane-diol, dithiothreitol, EGTA, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.30 Å R-free 0.257
1JX2 CRYSTAL STRUCTURE OF THE NUCLEOTIDE-FREE DYNAMIN A GTPASE DOMAIN, DETERMINED AS MYOSIN FUSION 提交 2001-09-05	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 信息不足单体；蛋白 × 1 PDB 声明：monomeric	链 A 3–765（763 aa）片段：catalytic domain	未记录	BGC beta-D-glucopyranose × 1 MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 8.5；277 K；PEG 8000, Tris, potassium chloride, magnesium chloride, glucose, methyl-propane-diol, dithiothreitol, EGTA, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.30 Å R-free 0.255
1LVK X-RAY CRYSTAL STRUCTURE OF THE MG (DOT) 2'(3')-O-(N-METHYLANTHRANILOYL) NUCLEOTIDE BOUND TO DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN 提交 1997-09-05	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–759（759 aa）片段：MOTOR DOMAIN	突变：Q760L, R761P, I762N	MG MAGNESIUM ION × 1 MNT 2'(3')-O-N-METHYLANTHRANILOYL-ADENOSINE-5'-DIPHOSPHATE × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 1.90 Å
1MMA X-RAY STRUCTURES OF THE MGADP, MGATPGAMMAS, AND MGAMPPNP COMPLEXES OF THE DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN 提交 1997-07-18	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–759（759 aa）片段：MOTOR DOMAIN	突变：Q760L, R761P, I762N	MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 7；pH 7.0	分辨率 2.10 Å
1MMD TRUNCATED HEAD OF MYOSIN FROM DICTYOSTELIUM DISCOIDEUM COMPLEXED WITH MGADP-BEF3 提交 1995-03-21	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–759（759 aa）片段：MOTOR DOMAIN	突变：Q760L, R761P, I762N	MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.00 Å
1MMG X-RAY STRUCTURES OF THE MGADP, MGATPGAMMAS, AND MGAMPPNP COMPLEXES OF THE DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN 提交 1997-07-18	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–759（759 aa）片段：MOTOR DOMAIN	突变：Q760L, R761P, I762N	MG MAGNESIUM ION × 1 AGS PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 7；pH 7.0	分辨率 2.10 Å
1MMN X-RAY STRUCTURES OF THE MGADP, MGATPGAMMAS, AND MGAMPPNP COMPLEXES OF THE DICTYOSTELIUM DISCOIDEUM MYOSIN MOTOR DOMAIN 提交 1997-07-18	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–759（759 aa）片段：MOTOR DOMAIN	突变：Q760L, R761P, I762N	MG MAGNESIUM ION × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 7；pH 7.0	分辨率 2.10 Å
1MND TRUNCATED HEAD OF MYOSIN FROM DICTYOSTELIUM DISCOIDEUM COMPLEXED WITH MGADP-ALF4 提交 1995-04-15	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–759（759 aa）片段：MOTOR DOMAIN	突变：Q760L, R761P, I762N	MG MAGNESIUM ION × 1 ALF TETRAFLUOROALUMINATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.60 Å
1MNE TRUNCATED HEAD OF MYOSIN FROM DICTYOSTELIUM DISCOIDEUM COMPLEXED WITH MG-PYROPHOSPHATE 提交 1995-04-20	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–759（759 aa）片段：MOTOR DOMAIN	突变：Q760L, R761P, I762N	MG MAGNESIUM ION × 1 POP PYROPHOSPHATE 2- × 1	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 2.70 Å
1VOM COMPLEX BETWEEN DICTYOSTELIUM MYOSIN AND MGADP AND VANADATE AT 1.9A RESOLUTION 提交 1995-11-09	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–759（759 aa）片段：TRUNCATED AT RESIDUE 762	未记录	MG MAGNESIUM ION × 1 VO4 VANADATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION mmCIF 未提供已读取条件	分辨率 1.90 Å
1W9I Myosin II Dictyostelium discoideum motor domain S456Y bound with MgADP-BeFx 提交 2004-10-13	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–759（759 aa）片段：MOTOR DOMAIN, RESIDUES 1-754	突变：YES	MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 EDO 1,2-ETHANEDIOL × 15	X-RAY DIFFRACTION X-ray结晶条件 pH 7；10% PEG 8000, 50 MM HEPES PH 7.0, 100 MM NACL, 2 MM DTT, 2 MM NAN3 IN THE RESERVOIR AND 7% PEG 8000, 50 MM HEPES PH 7.0, 100 MM NACL, 2 MM DTT, 2 MM NAN3 IN THE DROP	分辨率 1.75 Å R-free 0.207
1W9J Myosin II Dictyostelium discoideum motor domain S456Y bound with MgADP-AlF4 提交 2004-10-13	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–758（758 aa）片段：MOTOR DOMAIN, RESIDUES 1-755	突变：YES	MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ALF TETRAFLUOROALUMINATE ION × 1 EDO 1,2-ETHANEDIOL × 5	X-RAY DIFFRACTION X-ray结晶条件 pH 7；12% PEG 8000, 50 MM HEPES PH 7, 100 MM NACL, 2 MM DTT, 2 MM NAN3	分辨率 2.00 Å R-free 0.225
1W9K Dictyostelium discoideum Myosin II motor domain S456E with bound MgADP-BeFx 提交 2004-10-13	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–759（759 aa）片段：MOTOR DOMAIN, RESIDUES 1-759	突变：YES	MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 EDO 1,2-ETHANEDIOL × 7	X-RAY DIFFRACTION X-ray结晶条件 pH 7；10% PEG 8000, 50 MM HEPES PH 7, 100 MM NACL, 2 MM DTT, 2 MM NAN3 BY MICROSEEDING	分辨率 2.05 Å R-free 0.232
1W9L Myosin II Dictyostelium discoideum motor domain S456E bound with MgADP-AlF4 提交 2004-10-13	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–759（759 aa）片段：MOTOR DOMAIN, RESIDUES 1-759	突变：YES	MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 ALF TETRAFLUOROALUMINATE ION × 1 EDO 1,2-ETHANEDIOL × 6	X-RAY DIFFRACTION X-ray结晶条件 pH 7；12% PEG 8000, 50 MM HEPES PH 7, 100 MM NACL, 2 MM DTT, 2 MM NAN3	分辨率 1.95 Å R-free 0.225
1YV3 The structural basis of blebbistatin inhibition and specificity for myosin II 提交 2005-02-14	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 1–762（762 aa）	未记录	MG MAGNESIUM ION × 2 VO4 VANADATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 BIT (-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE × 1 EDO 1,2-ETHANEDIOL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；278 K；100 mM MOPS (pH 7.0), 250 mM MgCl2, 11% PEG 8000, 1 mM TCEP, 2 mM Thymol, 1 mM MgCl2, 2 mM ADP, and 3 mM sodium vanadate, VAPOR DIFFUSION, HANGING DROP, temperature 278K	分辨率 2.00 Å R-free 0.215
2AKA Structure of the nucleotide-free myosin II motor domain from Dictyostelium discoideum fused to the GTPase domain of dynamin 1 from Rattus norvegicus 提交 2005-08-03	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白异源复合物异源复合物；蛋白 × 3 PDB 声明：trimeric	链 A 2–765（764 aa）	未记录	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；Tris, PEG 8000, MPD, DTT, magnesium chloride, pH 8.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 1.90 Å R-free 0.224
2JHR Crystal structure of myosin-2 motor domain in complex with ADP- metavanadate and pentabromopseudilin 提交 2008-03-25	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 2–761（760 aa）片段：MOTOR-DOMAIN, RESIDUES 2-761	未记录	AD9 ADP METAVANADATE × 1 MG MAGNESIUM ION × 1 PBQ PENTABROMOPSEUDILIN × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 7.4；50 MM HEPES PH 7.4, 140 MM NACL, 11% W/V PEG8000, 2% (V/V) MPD, 5 MM MGCL2, 5 MM DTT, 1 MM EGTA.	分辨率 2.80 Å R-free 0.265
2JJ9 Crystal structure of myosin-2 in complex with ADP-metavanadate 提交 2008-03-25	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 2–761（760 aa）片段：MOTOR-DOMAIN, RESIDUES 2-761	未记录	AD9 ADP METAVANADATE × 1 MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 7.4；50 MM HEPES PH 7.4, 140 MM NACL, 11% W/V PEG8000, 2% (V/V) MPD, 5 MM MGCL2, 5 MM DTT, 1 MM EGTA	分辨率 2.30 Å R-free 0.275
2X9H CRYSTAL STRUCTURE OF MYOSIN-2 MOTOR DOMAIN IN COMPLEX WITH ADP- METAVANADATE AND PENTACHLOROCARBAZOLE 提交 2010-03-19	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 2–696（695 aa）片段：MYOSIN MOTOR DOMAIN, RESIDUES 2-696	未记录	AD9 ADP METAVANADATE × 1 MG MAGNESIUM ION × 1 KI9 2,3,4,6,8-PENTACHLORO-9H-CARBAZOL-1-OL × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 7.4；50 MM TRIS-HCL PH 7.4, 140 MM NACL, 9% W/V PEG8000, 2% (V/V) MPD, 5 MM MGCL2, 5 MM DTT, 1 MM EGTA	分辨率 2.70 Å R-free 0.289
2XEL Molecular Mechanism of Pentachloropseudilin Mediated Inhibition of Myosin Motor Activity 提交 2010-05-16	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 2–761（760 aa）片段：MOTOR DOMAIN, RESUDES 2-761	未记录	AD9 ADP METAVANADATE × 1 MG MAGNESIUM ION × 1 IA2 2,4-DICHLORO-6-(3,4,5-TRICHLORO-1H-PYRROL-2YL)PHENOL × 1	X-RAY DIFFRACTION X-ray结晶条件 50 MM HEPES PH 7.4, 140 MM NACL, 11% W/V PEG8000, 2% (V/V) MPD, 5 MM MGCL2, 5 MM DTT, 1 MM EGTA.	分辨率 2.50 Å R-free 0.253
2XO8 Crystal Structure of Myosin-2 in Complex with Tribromodichloropseudilin 提交 2010-08-10	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 3–761（759 aa）片段：MOTOR DOMAIN, RESIDUES 3-761	未记录	H70 2,4-DICHLORO-6-(3,4,5-TRIBROMO-1H-PYRROL-2-YL)PHENOL × 1 AD9 ADP METAVANADATE × 1 MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 6.8；50 MM HEPES(PH 6.8), 110 MM NACL, 11% PEG 5K-MME, 2% MPD, 5MM DTT, 1MM EGTA,5MM MGCL2	分辨率 2.40 Å R-free 0.254
2Y0R Structural basis for the allosteric interference of myosin function by mutants G680A and G680V of Dictyostelium myosin-2 提交 2010-12-07	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 X 2–759（758 aa）片段：MOTOR DOMAIN, RESIDUES 2-759	突变：YES	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 6.5；100 MM MES (PH 6.5), 25% PEG 8000.	分辨率 2.85 Å R-free 0.301
2Y8I Structural basis for the allosteric interference of myosin function by mutants G680A and G680V of Dictyostelium myosin-2 提交 2011-02-07	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 X 2–759（758 aa）片段：MOTOR DOMAIN, RESIDUES 2-759	突变：YES	ADP ADENOSINE-5'-DIPHOSPHATE × 1 MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray结晶条件 pH 7；13% PEG 6000, 4% GLYCEROL., pH 7.0	分辨率 3.13 Å R-free 0.337
2Y9E Structural basis for the allosteric interference of myosin function by mutants G680A and G680V of Dictyostelium myosin-2 提交 2011-02-14	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 X 2–759（758 aa）片段：MOTOR DOMAIN, RESIDUES 2-759	突变：YES	未记录非水小分子	X-RAY DIFFRACTION X-ray结晶条件 pH 7.5；100 MM HEPES (PH 7.5), 20% PEG10000	分辨率 3.40 Å R-free 0.372
3BZ7 Crystal Structures of (S)-(-)-Blebbistatin Analogs bound to Dictyostelium discoideum myosin II 提交 2008-01-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 2–759（758 aa）片段：motor domain, myosine head-like domain	未记录	MG MAGNESIUM ION × 2 VO4 VANADATE ION × 1 BL4 (3aS)-3a-hydroxy-5-methyl-1-phenyl-1,2,3,3a-tetrahydro-4H-pyrrolo[2,3-b]quinolin-4-one × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；278 K；mixing 5 ul of protein with an equal volume of well solution containing 100 mM MOPS, 250 mM MgCl2, 12% PEG 8000, 1 mM TCEP, and 2 mM Thymol, pH 7.0, vapor diffusion, hanging drop, temperature 278K	分辨率 2.00 Å R-free 0.223
3BZ7 Crystal Structures of (S)-(-)-Blebbistatin Analogs bound to Dictyostelium discoideum myosin II 提交 2008-01-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 2–759（758 aa）片段：motor domain, myosine head-like domain	未记录	MG MAGNESIUM ION × 4 VO4 VANADATE ION × 2 BL4 (3aS)-3a-hydroxy-5-methyl-1-phenyl-1,2,3,3a-tetrahydro-4H-pyrrolo[2,3-b]quinolin-4-one × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；278 K；mixing 5 ul of protein with an equal volume of well solution containing 100 mM MOPS, 250 mM MgCl2, 12% PEG 8000, 1 mM TCEP, and 2 mM Thymol, pH 7.0, vapor diffusion, hanging drop, temperature 278K	分辨率 2.00 Å R-free 0.223
3BZ8 Crystal Structures of (S)-(-)-Blebbistatin Analogs bound to Dictyostelium discoideum myosin II 提交 2008-01-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 2–759（758 aa）片段：Motor domain, Myosine head-like domain	未记录	MG MAGNESIUM ION × 2 VO4 VANADATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 EDO 1,2-ETHANEDIOL × 1 BL6 (3aS)-3a-hydroxy-7-methyl-1-phenyl-1,2,3,3a-tetrahydro-4H-pyrrolo[2,3-b]quinolin-4-one × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；278 K；mixing 5 ul of protein with an equal volume of well solution containing 100 mM MOPS, 250 mM MgCl2, 12% PEG 8000, 1 mM TCEP, and 2 mM Thymol, pH 7.0, vapor diffusion, hanging drop, temperature 278K	分辨率 2.20 Å R-free 0.245
3BZ8 Crystal Structures of (S)-(-)-Blebbistatin Analogs bound to Dictyostelium discoideum myosin II 提交 2008-01-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 2–759（758 aa）片段：Motor domain, Myosine head-like domain	未记录	MG MAGNESIUM ION × 4 VO4 VANADATE ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 EDO 1,2-ETHANEDIOL × 2 BL6 (3aS)-3a-hydroxy-7-methyl-1-phenyl-1,2,3,3a-tetrahydro-4H-pyrrolo[2,3-b]quinolin-4-one × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；278 K；mixing 5 ul of protein with an equal volume of well solution containing 100 mM MOPS, 250 mM MgCl2, 12% PEG 8000, 1 mM TCEP, and 2 mM Thymol, pH 7.0, vapor diffusion, hanging drop, temperature 278K	分辨率 2.20 Å R-free 0.245
3BZ9 Crystal Structures of (S)-(-)-Blebbistatin Analogs bound to Dictyostelium discoideum myosin II 提交 2008-01-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 2–759（758 aa）片段：Motor domain, Myosine head-like domain	未记录	MG MAGNESIUM ION × 2 VO4 VANADATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 BL7 (3aS)-3a-hydroxy-1-phenyl-1,2,3,3a-tetrahydro-4H-pyrrolo[2,3-b]quinolin-4-one × 1 EDO 1,2-ETHANEDIOL × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；278 K；mixing 5 ul of protein with an equal volume of well solution containing 100 mM MOPS, 250 mM MgCl2, 12% PEG 8000, 1 mM TCEP, and 2 mM Thymol, pH 7.0, vapor diffusion, hanging drop, temperature 278K	分辨率 2.10 Å R-free 0.238
3BZ9 Crystal Structures of (S)-(-)-Blebbistatin Analogs bound to Dictyostelium discoideum myosin II 提交 2008-01-17	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白同源多聚体同源多聚体；蛋白 × 2 PDB 声明：dimeric	链 A 2–759（758 aa）片段：Motor domain, Myosine head-like domain	未记录	MG MAGNESIUM ION × 4 VO4 VANADATE ION × 2 ADP ADENOSINE-5'-DIPHOSPHATE × 2 BL7 (3aS)-3a-hydroxy-1-phenyl-1,2,3,3a-tetrahydro-4H-pyrrolo[2,3-b]quinolin-4-one × 2 EDO 1,2-ETHANEDIOL × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；278 K；mixing 5 ul of protein with an equal volume of well solution containing 100 mM MOPS, 250 mM MgCl2, 12% PEG 8000, 1 mM TCEP, and 2 mM Thymol, pH 7.0, vapor diffusion, hanging drop, temperature 278K	分辨率 2.10 Å R-free 0.238
3MJX Crystal structure of myosin-2 motor domain in complex with ADP-Metavanadate and blebbistatin 提交 2010-04-13	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 2–761（760 aa）	未记录	AD9 ADP METAVANADATE × 1 MG MAGNESIUM ION × 1 BIT (-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；pH 7.4；277 K；50 MM HEPES PH 7.4, 140 MM NACL, 11% W/V PEG8000, 2% (V/V) MPD, 5 MM MGCL2, 5 MM DTT, 1 MM EGTA, 4mM ADP, 4mM meta-vanadate, 0.2 mM blebbistatin., VAPOR DIFFUSION, temperature 277K	分辨率 2.20 Å R-free 0.226
3MKD Crystal structure of myosin-2 dictyostelium discoideum motor domain S456Y mutant in complex with adp-orthovanadate 提交 2010-04-14	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 2–693（692 aa）片段：Myosin-2 motor domain, UNP residues 2-693	突变：S456Y	MG MAGNESIUM ION × 1 VO4 VANADATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7.4；278 K；50mM HEPES, 140mM sodium chloride, 11%(w/v) PEG 5000, 5mM magnesium chloride, 5mM DTT, 1mM EGTA, 2%(v/v) MPD, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 278K	分辨率 2.40 Å R-free 0.240
3MNQ Crystal structure of myosin-2 motor domain in complex with ADP-metavanadate and resveratrol 提交 2010-04-22	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 3–761（759 aa）片段：motor domain, residues 3-761	未记录	AD9 ADP METAVANADATE × 1 MG MAGNESIUM ION × 1 STL RESVERATROL × 1 EDO 1,2-ETHANEDIOL × 4	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.6；277 K；50mM Tris 7.6, 9% PEG 5000 MME, 140mM NaCl, 2% MPD, 5mM MgCl2, 5mM DTT, 1mM EGTA, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.20 Å R-free 0.250
3MYH Insights into the Importance of Hydrogen Bonding in the Gamma-Phosphate Binding Pocket of Myosin: Structural and Functional Studies of Ser236 提交 2010-05-10	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 X 2–759（758 aa）	突变：S236A	MG MAGNESIUM ION × 1 VO4 VANADATE ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 BIT (-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；12% PEG8K, 250mM MgCl2, 100mM MOPS, 2mM ADP, 3mM sodium vanadate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.01 Å R-free 0.240
3MYK Insights into the Importance of Hydrogen Bonding in the Gamma-Phosphate Binding Pocket of Myosin: Structural and Functional Studies of Ser236 提交 2010-05-10	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 X 2–759（758 aa）	突变：S236A	BIT (-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE × 1 MG MAGNESIUM ION × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；12% PEG8K, 250mM MgCl2, 100mM MOPS, 2mM AMPPNP, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 1.84 Å R-free 0.230
3MYL Insights into the Importance of Hydrogen Bonding in the Gamma-Phosphate Binding Pocket of Myosin: Structural and Functional Studies of Ser236 提交 2010-05-10	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 X 2–759（758 aa）	突变：S236A	MG MAGNESIUM ION × 1 POP PYROPHOSPHATE 2- × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；12.5% MePEG5K, 117mM ammonium acetate, 100mM Hepps, 2mM MgCl2, 2mM sodium pyrophosphate , pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K	分辨率 2.00 Å R-free 0.244
4AE3 Crystal structure of ammosamide 272:myosin-2 motor domain complex 提交 2012-01-05	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 2–761（760 aa）片段：MOTOR DOMAIN, RESIDUES 2-761	未记录	AOV ADP ORTHOVANADATE × 1 MG MAGNESIUM ION × 1 27X AMMOSAMIDE 272 × 2 EDO 1,2-ETHANEDIOL × 4	X-RAY DIFFRACTION X-ray结晶条件 50 MM HEPES PH 7.6, 140 MM NACL, 11% W/V PEG5K-MME, 2% (V/V) MPD	分辨率 2.50 Å R-free 0.254
4PJK Dicty myosin II R238E.E459R mutant (with ADP.Pi) in the Pi release state 提交 2014-05-12	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：Monomeric	链 A 1–761（761 aa）	突变：R238E, E459R	MG MAGNESIUM ION × 1 ADP ADENOSINE-5'-DIPHOSPHATE × 1 PO4 PHOSPHATE ION × 2 GOL GLYCEROL × 7	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 7.5；277 K；11,25% PEG8k, 100mM MOPS pH 7,5, 1mM TCEP, 250mM MgCl2, 50mM NaH2PO4	分辨率 2.15 Å R-free 0.213
6Z2S Crystal structure of adhibin analogue-bound myosin-2 提交 2020-05-18	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 2–761（760 aa）	未记录	MG MAGNESIUM ION × 1 Q5Q 3,6-dibromo-1-(hydroxymethyl)carbazole × 1 AD9 ADP METAVANADATE × 1 ACT ACETATE ION × 3 GOL GLYCEROL × 5	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION；277 K；0.2 M lithium acetate and 20 % PEG 3350	分辨率 3.20 Å R-free 0.262
6Z7T Nucleotide-free Myosin-II motor domain 提交 2020-06-01	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 2–761（760 aa）	未记录	MG MAGNESIUM ION × 2 EDO 1,2-ETHANEDIOL × 10 GOL GLYCEROL × 5	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；100 mM HEPES, pH7.5, 10% PEG8000, 8% Ethylenglycol	分辨率 1.88 Å R-free 0.217
6Z7T Nucleotide-free Myosin-II motor domain 提交 2020-06-01	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 2 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 B 2–761（760 aa）	未记录	MG MAGNESIUM ION × 2 EDO 1,2-ETHANEDIOL × 10 GOL GLYCEROL × 5 PGE TRIETHYLENE GLYCOL × 1 PEG DI(HYDROXYETHYL)ETHER × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；100 mM HEPES, pH7.5, 10% PEG8000, 8% Ethylenglycol	分辨率 1.88 Å R-free 0.217
6Z7U Myosin-II motor domain complexed with blebbistatin in a new ADP-release conformation 提交 2020-06-01	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 2–761（760 aa）	未记录	EDO 1,2-ETHANEDIOL × 9 ADP ADENOSINE-5'-DIPHOSPHATE × 1 BIT (-)-1-PHENYL-1,2,3,4-TETRAHYDRO-4-HYDROXYPYRROLO[2,3-B]-7-METHYLQUINOLIN-4-ONE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, SITTING DROP；pH 7.5；277 K；0.1 M HEPES, pH7.5, 10% PEG8000, 8% ethylene glycol	分辨率 2.58 Å R-free 0.231
7B19 Mutant Myosin-II-GGG motor domain 提交 2020-11-24	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 蛋白单体单体；蛋白 × 1 PDB 声明：monomeric	链 A 2–646（645 aa）链 A 649–761（113 aa）	未记录	AD9 ADP METAVANADATE × 1 MG MAGNESIUM ION × 1 GOL GLYCEROL × 6 GLC alpha-D-glucopyranose × 1 MLI MALONATE ION × 5 PEG DI(HYDROXYETHYL)ETHER × 2	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；0.24 M sodium malonate, pH8.0, 27% PEG 3350	分辨率 2.55 Å R-free 0.252
7B1A Myosin-II-AA mutant motor domain 提交 2020-11-24	构建体不同突变/修饰不同聚集状态不同配体/离子不同实验环境不同结构质量不同	Assembly 1 其他组合单体；蛋白 × 1 PDB 声明：monomeric	链 A 2–761（760 aa）	未记录	MG MAGNESIUM ION × 1 EDO 1,2-ETHANEDIOL × 3 MLI MALONATE ION × 1 AD9 ADP METAVANADATE × 1	X-RAY DIFFRACTION X-ray结晶条件 VAPOR DIFFUSION, HANGING DROP；pH 6.5；277 K；0.24 M sodium malonate pH6.5, 21% PEG 3350	分辨率 2.60 Å R-free 0.263

共 48 个其他 PDB 条目、52 个 assembly。打开独立比较页并筛选聚集状态

查看构建体与数据证据

UniProt名称	MYS2_DICDI
Isoform	—
PDB实体	1
链与序列区间	作者链 A； PDB构建体 1–761； UniProt 1–761 作者链 B； PDB构建体 1–761； UniProt 1–761

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。链映射与序列区间收在“数据证据”中；数据库内部编号、导入时间和 assembly 操作表达式仅用于维护，因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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2. 结构基本信息 2. Structure Basics

条目编号 entry_id	1g8x
沉积日期 deposition_date	2000-11-21
结构标题 title	STRUCTURE OF A GENETICALLY ENGINEERED MOLECULAR MOTOR
关键词 keywords	myosin, motor, alpha-actinin, dictyostelium, lever arm, protein engineering, structural protein; STRUCTURAL PROTEIN
实验方法 method	X-RAY DIFFRACTION

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3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	58.91 Å
回转半径 Rg (电子) rg_electron	60.03 Å
零角强度 I(0) i0	778198000.00
分子量 molecular_weight	230700.0 kDa
排除体积 excluded_volume	288590 Å³
包络体积 envelope_volume	493680 Å³
水化壳体积 shell_volume	76177 Å³
包络直径 envelope_diameter	294.2 Å
壳层 Rg shell_rg	54.26 Å
包络 Rg envelope_rg	63.17 Å
形状 Rg shape_rg	60.08 Å
总 Rg total_rg	59.66 Å
总原子数 total_atoms	16278
残基数 n_residues	2018
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	210.4 Å
Rg (实空间) rg_real	59.26 Å
Rg 误差 (实空间) rg_real_error	2.34 Å
I(0) (实空间) i0_real	7.7560e+08
I(0) 误差 (实空间) i0_real_error	1.7990e+07
Rg (倒空间) rg_reciprocal	57.83 Å
I(0) (倒空间) i0_reciprocal	775300000.0000
解质量估计 total_estimate	0.5338
解质量评级 solution_quality	REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	54.5 Å
偏度 Skewness skewness	0.731
峰度 Kurtosis kurtosis	0.036
角度范围 angular_range	— – 0.1350 Å⁻¹
当前正则化参数 α current_alpha	0.0060
最高正则化参数 α highest_alpha	45330000.0000
实空间数据点数 n_real_points	28
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.001; Oscil: 0.587; Stabil: 0.998; Sysdev: 0.009; Positv: 1.000; Valcen: 0.623; Smooth: 0.523

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5. 晶体学与实验 5. Crystallography & Experiment

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6. 实体与聚合物信息 Entities & Polymers (4)

实体 1 Entity 1 polymer

描述 description	MYOSIN II HEAVY CHAIN FUSED TO ALPHA-ACTININ 3
分子量 formula_weight	115208.9 kDa
突变 mutation	ARG238GLU
来源方法 src_method	man
拷贝数 entity_count	2

聚合物 Polymer

聚合物类型 type	polypeptide(L)
链编号 strand_id	A,B
原子数 atom_count	16292

MNPIHDRTSDYHKYLKVKQGDSDLFKLTVSDKRYIWYNPDPDERDSYECGEIVSETSDSFTFKTVDGQDRQVKKDDANQRNPIKFDGVEDMSELSYLNEPAVFHNLRVRYNQDLIYTYSGLFLVAVNPFKRIPIYTQEMVDIFKGRRRNEVAPHIFAISDVAYRSMLDDRQNQSLLITGESGAGKTENTKKVIQYLASVAGRNQANGSGVLEQQILQANPILEAFGNAKTTRNNNSSEFGKFIEIQFNNAGFISGASIQSYLLEKSRVVFQSETERNYHIFYQLLAGATAEEKKALHLAGPESFNYLNQSGCVDIKGVSDEDEFKITRQAMDIVGFSQEEQMSIFKIIAGILHLGNIKFEKGAGEGAVLKDKTALNAASTVFGVNPSVLEKALMEPRILAGRDLVAQHLNVEKSSSSRDALVKALYGRLFLWLVKKINNVLCSERKAYFIGVLDISGFEIFKVNSFEQLCINYTNEKLQQFFNHHMFKVEQEEYLKEKINWTFIDFGLDSQATIDLIDGRQPPGILALLDEQSVFPNATDNTLITKLHSHFSKKNAKYEEPRFSKTEFGVTHYAGQVMYEIQDWLEKNKDPLQQDLELCFKDSSDNVVTKLFNDPNIASRAKKGANFITVAAQYKEQLASLMATLETTNPHFVRCIIPNNKQLPAKLEDKVVLDQLRCNGVLEGIRITRKGFPNRIIYADFVKRYYLLAPNVPRDAEDSQKATDAVLKHLNIDPEQYRFGITKIFFRAGQLARIEEAREQRLGSEQTKSDYLKRANELVQWINDKQASLESRDFGDSIESVQSFMNAHKEYKKTEKPPKGQEVSELEAIYNSLQTKLRLIKREPFVAPAGLTPNEIDSTWSALEKAEQEHAEALRIELKRQKKIAVLLQKYNRILKKLENWATTKSVYLGSNETGDSITAVQAKLKNLEAFDGECQSLEGQSNSDLLSILAQLTELNYNGVPELTERKDTFFAQQWTGVKSSAETYKNTLLAELERLQKIEDLHHHHHHH

来源 Source

来源生物 organism	Dictyostelium discoideum
NCBI 分类编号 ncbi_taxonomy_id	44689
宿主生物 host_organism	Dictyostelium discoideum

实体 2 Entity 2 non-polymer

描述 description	MAGNESIUM ION
分子量 formula_weight	24.3 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

实体 3 Entity 3 non-polymer

描述 description	ADENOSINE-5'-DIPHOSPHATE
分子量 formula_weight	427.2 kDa
来源方法 src_method	syn
拷贝数 entity_count	2

实体 4 Entity 4 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	14

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7. 折叠分类 (SCOP + CATH) 16 domains

SCOP 2.08 (4 domains)

结构域编号 domain_id	d1g8xa1
类 Class class	k — Designed proteins
折叠类型 Fold fold	k.1 — Hybrid and chimeric proteins
超家族 Superfamily superfamily	k.1.1 — Hybrid and chimeric proteins
家族 Family family	k.1.1.1 — Hybrid and chimeric proteins

结构域编号 domain_id	d1g8xa2
类 Class class	l — Artifacts
折叠类型 Fold fold	l.1 — Tags
超家族 Superfamily superfamily	l.1.1 — Tags
家族 Family family	l.1.1.1 — Tags

结构域编号 domain_id	d1g8xb1
类 Class class	k — Designed proteins
折叠类型 Fold fold	k.1 — Hybrid and chimeric proteins
超家族 Superfamily superfamily	k.1.1 — Hybrid and chimeric proteins
家族 Family family	k.1.1.1 — Hybrid and chimeric proteins

结构域编号 domain_id	d1g8xb2
类 Class class	l — Artifacts
折叠类型 Fold fold	l.1 — Tags
超家族 Superfamily superfamily	l.1.1 — Tags
家族 Family family	l.1.1.1 — Tags

CATH v4.4 (12 domains)

结构域编号 domain_id	1g8xA01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	360 — Myosin S1 fragment, N-terminal

结构域编号 domain_id	1g8xA02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	850 — Kinesin
同源超家族 H-superfamily homologous superfamily	10 — Kinesin motor domain

结构域编号 domain_id	1g8xA03
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily	820 —

结构域编号 domain_id	1g8xA04
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	120 — Four Helix Bundle (Hemerythrin (Met), subunit A)
同源超家族 H-superfamily homologous superfamily	720 — Myosin VI head, motor domain, U50 subdomain

结构域编号 domain_id	1g8xA05
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily	530 —

结构域编号 domain_id	1g8xA06
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily	60 —

结构域编号 domain_id	1g8xB01
类 Class class	2 — Mainly Beta
架构 Architecture architecture	30 — Roll
拓扑 Topology topology	30 — SH3 type barrels.
同源超家族 H-superfamily homologous superfamily	360 — Myosin S1 fragment, N-terminal

结构域编号 domain_id	1g8xB02
类 Class class	3 — Alpha Beta
架构 Architecture architecture	40 — 3-Layer(aba) Sandwich
拓扑 Topology topology	850 — Kinesin
同源超家族 H-superfamily homologous superfamily	10 — Kinesin motor domain

结构域编号 domain_id	1g8xB03
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	10 — Orthogonal Bundle
拓扑 Topology topology	10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily	820 —

结构域编号 domain_id	1g8xB04
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	120 — Four Helix Bundle (Hemerythrin (Met), subunit A)
同源超家族 H-superfamily homologous superfamily	720 — Myosin VI head, motor domain, U50 subdomain

结构域编号 domain_id	1g8xB05
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily	530 —

结构域编号 domain_id	1g8xB06
类 Class class	1 — Mainly Alpha
架构 Architecture architecture	20 — Up-down Bundle
拓扑 Topology topology	58 — Methane Monooxygenase Hydroxylase; Chain G, domain 1
同源超家族 H-superfamily homologous superfamily	60 —

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8. 引用文献 (1)

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晶胞边长 a length_a	135.423 Å
晶胞边长 b length_b	155.420 Å
晶胞边长 c length_c	143.191 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	8

空间群 space_group	P 21 21 2
空间群编号 int_tables_number	18
晶系 crystal_system	—

分辨率 resolution	2.800 Å
最低分辨率 resolution_low	41.150 Å
R 因子 r_factor	0.2320
R_work r_work	0.2320
R_free r_free	0.2900

fit	/app/data/saxs_computation/g8/1g8x/1g8x.fit
pddf	/app/data/pr_computation/g8/1g8x/1g8x.pr.out
profile	/app/data/saxs_computation/g8/1g8x/1g8x.dat

STRUCTURE OF A GENETICALLY ENGINEERED MOLECULAR MOTOR

1. 蛋白身份与相关结构 Protein Identity & Related Structures

MYOSIN II HEAVY CHAIN FUSED TO ALPHA-ACTININ 3

当前结构中的状态

数据库中的同蛋白其他状态

MYOSIN II HEAVY CHAIN FUSED TO ALPHA-ACTININ 3

当前结构中的状态

数据库中的同蛋白其他状态

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

2. 结构基本信息 2. Structure Basics

3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

5. 晶体学与实验 5. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

6. 实体与聚合物信息 Entities & Polymers (4)

实体 1 Entity 1 polymer

聚合物 Polymer

来源 Source

实体 2 Entity 2 non-polymer

实体 3 Entity 3 non-polymer

实体 4 Entity 4 water

7. 折叠分类 (SCOP + CATH) 16 domains

SCOP 2.08 (4 domains)

CATH v4.4 (12 domains)

8. 引用文献 (1)

9. 文件与曲线 (10)

Files (7)

Curves (3)