AZURIN
Pseudomonas aeruginosa
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 21–148 | Mutation：F110S | CU COPPER (II) ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions | Resolution 2.30 Å |
| 10 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain K； UniProt 21–148 | Mutation：F110S | CU COPPER (II) ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions | Resolution 2.30 Å |
| 11 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain L； UniProt 21–148 | Mutation：F110S | CU COPPER (II) ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions | Resolution 2.30 Å |
| 12 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain M； UniProt 21–148 | Mutation：F110S | CU COPPER (II) ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions | Resolution 2.30 Å |
| 2 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain B； UniProt 21–148 | Mutation：F110S | CU COPPER (II) ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions | Resolution 2.30 Å |
| 3 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain C； UniProt 21–148 | Mutation：F110S | CU COPPER (II) ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions | Resolution 2.30 Å |
| 4 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain D； UniProt 21–148 | Mutation：F110S | CU COPPER (II) ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions | Resolution 2.30 Å |
| 5 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain E； UniProt 21–148 | Mutation：F110S | CU COPPER (II) ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions | Resolution 2.30 Å |
| 6 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain F； UniProt 21–148 | Mutation：F110S | CU COPPER (II) ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions | Resolution 2.30 Å |
| 7 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain G； UniProt 21–148 | Mutation：F110S | CU COPPER (II) ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions | Resolution 2.30 Å |
| 8 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain H； UniProt 21–148 | Mutation：F110S | CU COPPER (II) ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions | Resolution 2.30 Å |
| 9 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain I； UniProt 21–148 | Mutation：F110S | CU COPPER (II) ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions | Resolution 2.30 Å |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 1ILU | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 1AG0 STRUCTURE OF CYS 112 ASP AZURIN FROM PSEUDOMONAS AERUGINOSA Deposited 1997-03-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
20–148（129 aa）
|
Mutation：C113D | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.40 Å |
| 1AG0 STRUCTURE OF CYS 112 ASP AZURIN FROM PSEUDOMONAS AERUGINOSA Deposited 1997-03-26 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
20–148（129 aa）
|
Mutation：C113D | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6.5；pH 6.5
|
Resolution 2.40 Å |
| 1AZN CRYSTAL STRUCTURE OF THE AZURIN MUTANT PHE114ALA FROM PSEUDOMONAS AERUGINOSA AT 2.6 ANGSTROMS RESOLUTION Deposited 1994-05-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.60 Å |
| 1AZN CRYSTAL STRUCTURE OF THE AZURIN MUTANT PHE114ALA FROM PSEUDOMONAS AERUGINOSA AT 2.6 ANGSTROMS RESOLUTION Deposited 1994-05-27 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain C
21–148（128 aa）
Chain D
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.60 Å |
| 1AZR CRYSTAL STRUCTURE OF PSEUDOMONAS AERUGINOSA ZINC AZURIN MUTANT ASP47ASP AT 2.4 ANGSTROMS RESOLUTION Deposited 1993-03-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
Chain C
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 NO3 NITRATE ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.40 Å |
| 1AZR CRYSTAL STRUCTURE OF PSEUDOMONAS AERUGINOSA ZINC AZURIN MUTANT ASP47ASP AT 2.4 ANGSTROMS RESOLUTION Deposited 1993-03-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
21–148（128 aa）
Chain D
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.40 Å |
| 1AZU STRUCTURAL FEATURES OF AZURIN AT 2.7 ANGSTROMS RESOLUTION Deposited 1980-08-04 | Different mutation/modification Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.70 Å |
| 1BEX STRUCTURE OF RUTHENIUM-MODIFIED PSEUDOMONAS AERUGINOSA AZURIN Deposited 1998-05-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 4 RBU RUTHEMIUM BIS(2,2'-BIPYRIDINE)-2-IMIDAZOLE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；30% PEG 4000, 100 MM LINO3,20 MM CUCL2,100 MM TRIS PH 8.0
|
Resolution 2.30 Å R-free 0.289 |
| 1BEX STRUCTURE OF RUTHENIUM-MODIFIED PSEUDOMONAS AERUGINOSA AZURIN Deposited 1998-05-18 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 4 RBU RUTHEMIUM BIS(2,2'-BIPYRIDINE)-2-IMIDAZOLE × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8；30% PEG 4000, 100 MM LINO3,20 MM CUCL2,100 MM TRIS PH 8.0
|
Resolution 2.30 Å R-free 0.289 |
| 1CC3 PURPLE CUA CENTER Deposited 1999-03-03 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.1；pH 5.1
|
Resolution 1.65 Å R-free 0.263 |
| 1CC3 PURPLE CUA CENTER Deposited 1999-03-03 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.1；pH 5.1
|
Resolution 1.65 Å R-free 0.263 |
| 1E5Y Azurin from Pseudomonas aeruginosa, reduced form, pH 5.5 Deposited 2000-08-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
21–148（128 aa）
Chain D
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.5；PH 5.5
|
Resolution 2.00 Å |
| 1E5Y Azurin from Pseudomonas aeruginosa, reduced form, pH 5.5 Deposited 2000-08-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
Chain C
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.5；PH 5.5
|
Resolution 2.00 Å |
| 1E5Z Azurin from Pseudomonas aeruginosa, reduced form, pH 9.0 Deposited 2000-08-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
21–148（128 aa）
Chain D
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 9；PH 9.0
|
Resolution 2.00 Å |
| 1E5Z Azurin from Pseudomonas aeruginosa, reduced form, pH 9.0 Deposited 2000-08-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
Chain C
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 9；PH 9.0
|
Resolution 2.00 Å |
| 1E65 Azurin from Pseudomonas aeruginosa, apo form Deposited 2000-08-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
Chain C
21–148（128 aa）
Chain D
21–148（128 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.5；PH 5.5
|
Resolution 1.85 Å |
| 1E67 Zn-Azurin from Pseudomonas aeruginosa Deposited 2000-08-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | ZN ZINC ION × 1 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.7；pH 5.70
|
Resolution 2.14 Å |
| 1E67 Zn-Azurin from Pseudomonas aeruginosa Deposited 2000-08-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.7；pH 5.70
|
Resolution 2.14 Å |
| 1E67 Zn-Azurin from Pseudomonas aeruginosa Deposited 2000-08-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.7；pH 5.70
|
Resolution 2.14 Å |
| 1E67 Zn-Azurin from Pseudomonas aeruginosa Deposited 2000-08-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
|
Not recorded | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.7；pH 5.70
|
Resolution 2.14 Å |
| 1ETJ AZURIN MUTANT WITH MET 121 REPLACED BY GLU Deposited 1997-01-11 | Different mutation/modification Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：M121E | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；THE BLUISH WELL-FORMED PRISMATIC CRYSTALS OF THE TITLE PROTEIN WERE OBTAINED BY THE VAPOR-DIFFUSION HANGING-DROP TECHNIQUE FROM A SOLUTION CONTAINING 25% PEG4000, 0.24M CALCIUM DICHLORIDE AND 0.26M LITHIUM NITRATE BUFFER AT PH 6.0 AND AT THE TEMPERATURE OF 24 - 25 CENTIGRADE IN AROUND 10 DAYS., vapor diffusion - hanging drop
|
Resolution 2.30 Å |
| 1ETJ AZURIN MUTANT WITH MET 121 REPLACED BY GLU Deposited 1997-01-11 | Different mutation/modification Different experimental conditions | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：M121E | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；THE BLUISH WELL-FORMED PRISMATIC CRYSTALS OF THE TITLE PROTEIN WERE OBTAINED BY THE VAPOR-DIFFUSION HANGING-DROP TECHNIQUE FROM A SOLUTION CONTAINING 25% PEG4000, 0.24M CALCIUM DICHLORIDE AND 0.26M LITHIUM NITRATE BUFFER AT PH 6.0 AND AT THE TEMPERATURE OF 24 - 25 CENTIGRADE IN AROUND 10 DAYS., vapor diffusion - hanging drop
|
Resolution 2.30 Å |
| 1ETJ AZURIN MUTANT WITH MET 121 REPLACED BY GLU Deposited 1997-01-11 | Different mutation/modification Different experimental conditions | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
|
Mutation：M121E | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；THE BLUISH WELL-FORMED PRISMATIC CRYSTALS OF THE TITLE PROTEIN WERE OBTAINED BY THE VAPOR-DIFFUSION HANGING-DROP TECHNIQUE FROM A SOLUTION CONTAINING 25% PEG4000, 0.24M CALCIUM DICHLORIDE AND 0.26M LITHIUM NITRATE BUFFER AT PH 6.0 AND AT THE TEMPERATURE OF 24 - 25 CENTIGRADE IN AROUND 10 DAYS., vapor diffusion - hanging drop
|
Resolution 2.30 Å |
| 1ETJ AZURIN MUTANT WITH MET 121 REPLACED BY GLU Deposited 1997-01-11 | Different mutation/modification Different experimental conditions | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
|
Mutation：M121E | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；THE BLUISH WELL-FORMED PRISMATIC CRYSTALS OF THE TITLE PROTEIN WERE OBTAINED BY THE VAPOR-DIFFUSION HANGING-DROP TECHNIQUE FROM A SOLUTION CONTAINING 25% PEG4000, 0.24M CALCIUM DICHLORIDE AND 0.26M LITHIUM NITRATE BUFFER AT PH 6.0 AND AT THE TEMPERATURE OF 24 - 25 CENTIGRADE IN AROUND 10 DAYS., vapor diffusion - hanging drop
|
Resolution 2.30 Å |
| 1EZL CRYSTAL STRUCTURE OF THE DISULPHIDE BOND-DEFICIENT AZURIN MUTANT C3A/C26A: HOW IMPORTANT IS THE S-S BOND FOR FOLDING AND STABILITY? Deposited 2000-05-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
Chain C
21–148（128 aa）
Chain D
21–148（128 aa）
|
Mutation：C3A,C26A Mutation：C3A,C26A Mutation：C3A,C26A Mutation：C3A,C26A | CU COPPER (II) ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.7；295 K；ammonium sulphate, lithium nitrate, acetate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 2.00 Å R-free 0.256 |
| 1GR7 Crystal structure of the double mutant Cys3Ser/Ser100Pro from Pseudomonas Aeruginosa at 1.8 A resolution Deposited 2001-12-14 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
Chain C
21–148（128 aa）
Chain D
21–148（128 aa）
|
Mutation：YES Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：YES Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：YES Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：YES Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.5；3.2 M AMMONIUM SULPHATE, 1.0 M LITHIUM NITRATE, 0.2 M ACETATE BUFFER PH 5.5
|
Resolution 1.80 Å R-free 0.208 |
| 1I53 RE(I)-TRICARBONYL DIIMINE (Q107H)) AZURIN Deposited 2001-02-24 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：Q107H | CU COPPER (II) ION × 1 RTC RHENIUM (I) TRICARBONYL × 1 DPT 4,7-DIMETHYL-[1,10]PHENANTHROLINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；CuCl2, Imidazole, LiNo3, PEG 8K, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.80 Å R-free 0.259 |
| 1I53 RE(I)-TRICARBONYL DIIMINE (Q107H)) AZURIN Deposited 2001-02-24 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：Q107H | CU COPPER (II) ION × 1 RTC RHENIUM (I) TRICARBONYL × 1 DPT 4,7-DIMETHYL-[1,10]PHENANTHROLINE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；295 K；CuCl2, Imidazole, LiNo3, PEG 8K, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K
|
Resolution 1.80 Å R-free 0.259 |
| 1ILS X-RAY CRYSTAL STRUCTURE THE TWO SITE-SPECIFIC MUTANTS ILE7SER AND PHE110SER OF AZURIN FROM PSEUDOMONAS AERUGINOSA Deposited 1995-10-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
Chain C
21–148（128 aa）
|
Mutation：I7S Mutation：I7S | CU COPPER (II) ION × 2 NO3 NITRATE ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1ILS X-RAY CRYSTAL STRUCTURE THE TWO SITE-SPECIFIC MUTANTS ILE7SER AND PHE110SER OF AZURIN FROM PSEUDOMONAS AERUGINOSA Deposited 1995-10-12 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
21–148（128 aa）
Chain D
21–148（128 aa）
|
Mutation：I7S Mutation：I7S | CU COPPER (II) ION × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1JVL Azurin dimer, covalently crosslinked through bis-maleimidomethylether Deposited 2001-08-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
Fragment：Azurin
Chain B
21–148（128 aa）
Fragment：Azurin
|
Mutation：N42C Mutation：N42C | CU COPPER (II) ION × 2 NI NICKEL (II) ION × 2 144 TRIS-HYDROXYMETHYL-METHYL-AMMONIUM × 2 OPP 1-[PYRROL-1-YL-2,5-DIONE-METHOXYMETHYL]-PYRROLE-2,5-DIONE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；PEG 2000 MME, nickel chloride, Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 293K
|
Resolution 2.00 Å R-free 0.227 |
| 1JVO Azurin dimer, crosslinked via disulfide bridge Deposited 2001-08-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
21–148（128 aa）
Fragment：Azurin
Chain B
21–148（128 aa）
Fragment：Azurin
Chain C
21–148（128 aa）
Fragment：Azurin
Chain D
21–148（128 aa）
Fragment：Azurin
|
Mutation：N42C Mutation：N42C Mutation：N42C Mutation：N42C | CU COPPER (II) ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；PEG 8000, Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 293K, temperature 293.0K
|
Resolution 2.75 Å R-free 0.289 |
| 1JVO Azurin dimer, crosslinked via disulfide bridge Deposited 2001-08-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain E
21–148（128 aa）
Fragment：Azurin
Chain F
21–148（128 aa）
Fragment：Azurin
Chain G
21–148（128 aa）
Fragment：Azurin
Chain H
21–148（128 aa）
Fragment：Azurin
|
Mutation：N42C Mutation：N42C Mutation：N42C Mutation：N42C | CU COPPER (II) ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；PEG 8000, Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 293K, temperature 293.0K
|
Resolution 2.75 Å R-free 0.289 |
| 1JVO Azurin dimer, crosslinked via disulfide bridge Deposited 2001-08-30 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain I
21–148（128 aa）
Fragment：Azurin
Chain J
21–148（128 aa）
Fragment：Azurin
Chain K
21–148（128 aa）
Fragment：Azurin
Chain L
21–148（128 aa）
Fragment：Azurin
|
Mutation：N42C Mutation：N42C Mutation：N42C Mutation：N42C | CU COPPER (II) ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.5；293 K；PEG 8000, Tris/HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP at 293K, temperature 293.0K
|
Resolution 2.75 Å R-free 0.289 |
| 1JZE Pseudomonas aeruginosa Azurin Ru(bpy)2(im)(His83) Deposited 2001-09-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 DRU DELTA-BIS(2,2'-BIPYRIDINE)IMIDAZOLE RUTHENIUM (II) × 1 LRU LAMBDA-BIS(2,2'-BIPYRIDINE)IMIDAZOLE RUTHENIUM (II) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG, Imidazole, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.60 Å R-free 0.274 |
| 1JZF Pseudomonas aeruginosa Oxidized Azurin(Cu2+) Ru(tpy)(phen)(His83) Deposited 2001-09-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 RTB (2,2':6',2'-TERPYRIDINE)-(1,10-PHENANTHROLINE) RUTHENIUM (II) × 1 IME TETRA(IMIDAZOLE)DIAQUACOPPER (II) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG, Imidazole, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.50 Å R-free 0.220 |
| 1JZG Pseudomonas aeruginosa Reduced Azurin (Cu1+) Ru(tpy)(phen)(His83) Deposited 2001-09-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU1 COPPER (I) ION × 1 RTB (2,2':6',2'-TERPYRIDINE)-(1,10-PHENANTHROLINE) RUTHENIUM (II) × 1 IMF TETRA(IMIDAZOLE)DIAQUACOPPER (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG, Imidazole, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.40 Å R-free 0.232 |
| 1JZH Pseudomonas aeruginosa Azurin Ru(tpy)(bpy)(His83) Deposited 2001-09-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 RTA (2,2':6',2''-TERPYRIDINE)-(2,2''-BIPYRIDINE) RUTHENIUM (II) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；PEG, Imidazole, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.277 |
| 1JZI Pseudomonas aeruginosa Azurin Re(phen)(CO)3(His83) Deposited 2001-09-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 REP (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 IME TETRA(IMIDAZOLE)DIAQUACOPPER (II) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；PEG, Imidazole, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.62 Å R-free 0.247 |
| 1JZI Pseudomonas aeruginosa Azurin Re(phen)(CO)3(His83) Deposited 2001-09-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 REP (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；293 K；PEG, Imidazole, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.62 Å R-free 0.247 |
| 1JZJ Pseudomonas aeruginosa Azurin Os(bpy)2(im)(His83) Deposited 2001-09-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 DOS DELTA-BIS(2,2'-BIPYRIDINE)IMIDAZOLE OSMIUM (II) × 1 LOS LAMBDA-BIS(2,2'-BIPYRIDINE)IMIDAZOLE OSMIUM (II) × 1 IME TETRA(IMIDAZOLE)DIAQUACOPPER (II) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；PEG, Imidazole, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.80 Å R-free 0.260 |
| 1JZJ Pseudomonas aeruginosa Azurin Os(bpy)2(im)(His83) Deposited 2001-09-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 DOS DELTA-BIS(2,2'-BIPYRIDINE)IMIDAZOLE OSMIUM (II) × 1 LOS LAMBDA-BIS(2,2'-BIPYRIDINE)IMIDAZOLE OSMIUM (II) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；PEG, Imidazole, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.80 Å R-free 0.260 |
| 1JZJ Pseudomonas aeruginosa Azurin Os(bpy)2(im)(His83) Deposited 2001-09-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 6 DOS DELTA-BIS(2,2'-BIPYRIDINE)IMIDAZOLE OSMIUM (II) × 4 LOS LAMBDA-BIS(2,2'-BIPYRIDINE)IMIDAZOLE OSMIUM (II) × 4 IME TETRA(IMIDAZOLE)DIAQUACOPPER (II) × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；PEG, Imidazole, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.80 Å R-free 0.260 |
| 1JZJ Pseudomonas aeruginosa Azurin Os(bpy)2(im)(His83) Deposited 2001-09-16 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 3 DOS DELTA-BIS(2,2'-BIPYRIDINE)IMIDAZOLE OSMIUM (II) × 2 LOS LAMBDA-BIS(2,2'-BIPYRIDINE)IMIDAZOLE OSMIUM (II) × 2 IME TETRA(IMIDAZOLE)DIAQUACOPPER (II) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；PEG, Imidazole, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.80 Å R-free 0.260 |
| 1JZJ Pseudomonas aeruginosa Azurin Os(bpy)2(im)(His83) Deposited 2001-09-16 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 4 DOS DELTA-BIS(2,2'-BIPYRIDINE)IMIDAZOLE OSMIUM (II) × 2 LOS LAMBDA-BIS(2,2'-BIPYRIDINE)IMIDAZOLE OSMIUM (II) × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 6；298 K；PEG, Imidazole, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.80 Å R-free 0.260 |
| 1NZR CRYSTAL STRUCTURE OF THE AZURIN MUTANT NICKEL-TRP48MET FROM PSEUDOMONAS AERUGINOSA AT 2.2 ANGSTROMS RESOLUTION Deposited 1994-12-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
Chain C
21–148（128 aa）
|
Mutation：W48M Mutation：W48M | NI NICKEL (II) ION × 2 NO3 NITRATE ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1NZR CRYSTAL STRUCTURE OF THE AZURIN MUTANT NICKEL-TRP48MET FROM PSEUDOMONAS AERUGINOSA AT 2.2 ANGSTROMS RESOLUTION Deposited 1994-12-09 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
21–148（128 aa）
Chain D
21–148（128 aa）
|
Mutation：W48M Mutation：W48M | NI NICKEL (II) ION × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 1R1C PSEUDOMONAS AERUGINOSA W48F/Y72F/H83Q/Y108W-AZURIN RE(PHEN)(CO)3(HIS107) Deposited 2003-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：W48F/Y72F/H83Q/Q107H/Y108W | CU1 COPPER (I) ION × 1 REP (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；PEG 4000, lithium nitrate, imidazole, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.260 |
| 1R1C PSEUDOMONAS AERUGINOSA W48F/Y72F/H83Q/Y108W-AZURIN RE(PHEN)(CO)3(HIS107) Deposited 2003-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：W48F/Y72F/H83Q/Q107H/Y108W | CU1 COPPER (I) ION × 1 REP (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；PEG 4000, lithium nitrate, imidazole, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.260 |
| 1R1C PSEUDOMONAS AERUGINOSA W48F/Y72F/H83Q/Y108W-AZURIN RE(PHEN)(CO)3(HIS107) Deposited 2003-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
|
Mutation：W48F/Y72F/H83Q/Q107H/Y108W | CU1 COPPER (I) ION × 1 REP (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；PEG 4000, lithium nitrate, imidazole, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.260 |
| 1R1C PSEUDOMONAS AERUGINOSA W48F/Y72F/H83Q/Y108W-AZURIN RE(PHEN)(CO)3(HIS107) Deposited 2003-09-23 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
|
Mutation：W48F/Y72F/H83Q/Q107H/Y108W | CU1 COPPER (I) ION × 1 REP (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.5；298 K；PEG 4000, lithium nitrate, imidazole, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.260 |
| 1VLX STRUCTURE OF ELECTRON TRANSFER (COBALT-PROTEIN) Deposited 1996-10-08 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
Chain C
21–148（128 aa）
Chain D
21–148（128 aa）
|
Not recorded | CO COBALT (II) ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.7；THE BLUISH WELL-FORMED PRISMATIC CRYSTALS OF THE TITLE PROTEIN WERE OBTAINED BY THE VAPOR-DIFFUSION HANGING-DROP TECHNIQUE FROM A SOLUTION CONTAINING 3.6M AMMONIUM SULFATE, 0.5M LITHIUM NITRATE AND 0.1M ACETATE BUFFER AT PH 5.7 AND AT THE TEMPERATURE OF 24 - 25 CENTIGRADE IN AROUND 10 DAYS., vapor diffusion - hanging drop
|
Resolution 1.90 Å |
| 1XB3 The D62C/K74C double mutant of Pseudomonas Aeruginosa Azurin Deposited 2004-08-27 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：D62C/K74C | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；20-23% PEG 3350, 0.25M MgCl2, 0.1M Sodium Acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.50 Å R-free 0.214 |
| 1XB3 The D62C/K74C double mutant of Pseudomonas Aeruginosa Azurin Deposited 2004-08-27 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：D62C/K74C | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；20-23% PEG 3350, 0.25M MgCl2, 0.1M Sodium Acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.50 Å R-free 0.214 |
| 1XB6 The K24R mutant of Pseudomonas Aeruginosa Azurin Deposited 2004-08-30 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：K24R | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；20-23% PEG 3350, 0.25M MgCl2, 0.1M Sodium Acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.82 Å R-free 0.187 |
| 1XB6 The K24R mutant of Pseudomonas Aeruginosa Azurin Deposited 2004-08-30 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：K24R | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.5；293 K；20-23% PEG 3350, 0.25M MgCl2, 0.1M Sodium Acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.82 Å R-free 0.187 |
| 1XB8 Zn substituted form of D62C/K74C double mutant of Pseudomonas Aeruginosa Azurin Deposited 2004-08-30 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：D62C/K74C | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.2 M Ammonium Sulfate, 0.1 M Cacodylate, 30% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.00 Å R-free 0.236 |
| 1XB8 Zn substituted form of D62C/K74C double mutant of Pseudomonas Aeruginosa Azurin Deposited 2004-08-30 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
|
Mutation：D62C/K74C | ZN ZINC ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；0.2 M Ammonium Sulfate, 0.1 M Cacodylate, 30% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
|
Resolution 2.00 Å R-free 0.236 |
| 2AZU X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35*GLN AND HIS35*LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA Deposited 1991-01-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
Chain C
21–148（128 aa）
|
Mutation：H35L Mutation：H35L | CU COPPER (II) ION × 2 NO3 NITRATE ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 2AZU X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35*GLN AND HIS35*LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA Deposited 1991-01-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
21–148（128 aa）
Chain D
21–148（128 aa）
|
Mutation：H35L Mutation：H35L | CU COPPER (II) ION × 2 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 2FNW Pseudomonas aeruginosa E2Q/H83Q/M109H-azurin RE(PHEN)(CO)3 Deposited 2006-01-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
17–45（29 aa）
|
Mutation：E2Q/H83Q/M109H | CU COPPER (II) ION × 1 REP (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG molecular weight 4000, 100 mM LiNO3 and 100 mM imidazole pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.40 Å R-free 0.227 |
| 2FNW Pseudomonas aeruginosa E2Q/H83Q/M109H-azurin RE(PHEN)(CO)3 Deposited 2006-01-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
17–45（29 aa）
|
Mutation：E2Q/H83Q/M109H | CU COPPER (II) ION × 1 REP (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG molecular weight 4000, 100 mM LiNO3 and 100 mM imidazole pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.40 Å R-free 0.227 |
| 2FT6 Structure of Cu(II)azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CTPHPM" Deposited 2006-01-24 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；0.1MM MES, 20% PEG6000, 0.2M LiCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.25 Å R-free 0.155 |
| 2FT7 Structure of Cu(I)azurin at pH 6, with the metal-binding loop sequence "CTFPGHSALM" replaced with "CTPHPM" Deposited 2006-01-24 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU1 COPPER (I) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；0.1MM MES, 20% PEG6000, 0.2M LiCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.40 Å R-free 0.178 |
| 2FT8 Structure of Cu(I)azurin, pH8, with the metal-binding loop sequence "CTFPGHSALM" replaced with "CTPHPM" Deposited 2006-01-24 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU1 COPPER (I) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；293 K；0.1MM MES, 20% PEG6000, 0.2M LiCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.55 Å R-free 0.191 |
| 2FTA Structure of Cu(II)azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CTPHPFM" Deposited 2006-01-24 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 2PE NONAETHYLENE GLYCOL × 1 EOH ETHANOL × 3 PEG DI(HYDROXYETHYL)ETHER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.1M Potassium Thiocyanate, 30% PEG MME 2000, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.61 Å R-free 0.269 |
| 2FTA Structure of Cu(II)azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CTPHPFM" Deposited 2006-01-24 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.1M Potassium Thiocyanate, 30% PEG MME 2000, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.61 Å R-free 0.269 |
| 2FTA Structure of Cu(II)azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CTPHPFM" Deposited 2006-01-24 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.1M Potassium Thiocyanate, 30% PEG MME 2000, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.61 Å R-free 0.269 |
| 2FTA Structure of Cu(II)azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CTPHPFM" Deposited 2006-01-24 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 2PE NONAETHYLENE GLYCOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.1M Potassium Thiocyanate, 30% PEG MME 2000, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.61 Å R-free 0.269 |
| 2GHZ Crystal structure of Azurin Phe114Pro mutant Deposited 2006-03-28 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：Phe114Pro | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；25% PEG1500, 100mM MMT buffer, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.60 Å R-free 0.190 |
| 2GHZ Crystal structure of Azurin Phe114Pro mutant Deposited 2006-03-28 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：Phe114Pro | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；25% PEG1500, 100mM MMT buffer, pH 4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.60 Å R-free 0.190 |
| 2GI0 Crystal structure of Cu(I) Phe114Pro Azurin mutant Deposited 2006-03-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：Phe114Pro | CU1 COPPER (I) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；25% PEG1500, 100mM MMT buffer, pH4. To generate Cu(I), Cu(II) containing crystals were reduced by soaking in reservoir solution with 10mM ascorbate, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.224 |
| 2GI0 Crystal structure of Cu(I) Phe114Pro Azurin mutant Deposited 2006-03-28 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：Phe114Pro | CU1 COPPER (I) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4；293 K；25% PEG1500, 100mM MMT buffer, pH4. To generate Cu(I), Cu(II) containing crystals were reduced by soaking in reservoir solution with 10mM ascorbate, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.224 |
| 2HX7 Crystal structure of Cu(II) Azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CSPHQGAGM" Deposited 2006-08-03 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：;Metal binding loop "CTFPGHSALM" mutated to "CSPHQGAGM" ; | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；29-31% PEG 4000, 100mM magnesium chloride, 100mM Sodium acetate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.55 Å R-free 0.190 |
| 2HX7 Crystal structure of Cu(II) Azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CSPHQGAGM" Deposited 2006-08-03 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：;Metal binding loop "CTFPGHSALM" mutated to "CSPHQGAGM" ; | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；29-31% PEG 4000, 100mM magnesium chloride, 100mM Sodium acetate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.55 Å R-free 0.190 |
| 2HX8 Crystal structure of Cu(I) Azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CSPHQGAGM", at pH5 Deposited 2006-08-03 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：Metal binding loop | CU1 COPPER (I) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；29-31% PEG 4000, 100mM magnesium chloride, 100mM Sodium acetate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.60 Å R-free 0.184 |
| 2HX8 Crystal structure of Cu(I) Azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CSPHQGAGM", at pH5 Deposited 2006-08-03 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：Metal binding loop | CU1 COPPER (I) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；29-31% PEG 4000, 100mM magnesium chloride, 100mM Sodium acetate, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.60 Å R-free 0.184 |
| 2HX9 Crystal structure of Cu(I) Azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CSPHQGAGM", at pH4 Deposited 2006-08-03 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：Metal binding loop | CU1 COPPER (I) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；29-31% PEG 4000, 100mM magnesium chloride, 100mM Sodium acetate. Crystal soaked in pH4 buffer and ascorbate following growth, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.218 |
| 2HX9 Crystal structure of Cu(I) Azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CSPHQGAGM", at pH4 Deposited 2006-08-03 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：Metal binding loop | CU1 COPPER (I) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；29-31% PEG 4000, 100mM magnesium chloride, 100mM Sodium acetate. Crystal soaked in pH4 buffer and ascorbate following growth, pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.70 Å R-free 0.218 |
| 2HXA Crystal structure of Cu(I) Azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CSPHQGAGM", at pH3.5 Deposited 2006-08-03 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：Metal binding loop | CU1 COPPER (I) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；29-31% PEG 4000, 100mM magnesium chloride, 100mM Sodium acetate. Crystal soaked in ascorbate and pH3.5 buffer following growth, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.21 Å R-free 0.267 |
| 2HXA Crystal structure of Cu(I) Azurin with the metal-binding loop sequence "CTFPGHSALM" replaced with "CSPHQGAGM", at pH3.5 Deposited 2006-08-03 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：Metal binding loop | CU1 COPPER (I) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5；293 K；29-31% PEG 4000, 100mM magnesium chloride, 100mM Sodium acetate. Crystal soaked in ascorbate and pH3.5 buffer following growth, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.21 Å R-free 0.267 |
| 2I7O Structure of Re(4,7-dimethyl-phen)(Thr124His)(Lys122Trp)(His83Gln)AzCu(II), a Rhenium modified Azurin mutant Deposited 2006-08-31 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：H83N, K122W, T124H | CU COPPER (II) ION × 1 REQ (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 3.2；298 K；20-24% PEG 4000, 100 mM LiNO3, 100 mM citric acid, pH 3.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.50 Å R-free 0.255 |
| 2I7S Crystal structure of Re(phen)(CO)3 (Thr124His)(His83Gln) Azurin Cu(II) from Pseudomonas aeruginosa Deposited 2006-08-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
Chain C
21–148（128 aa）
|
Mutation：H83Q, T124H Mutation：H83Q, T124H | CU COPPER (II) ION × 2 REP (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG4000, 100 mM LiNO3, 100 mM Imidazole pH 7.0. One fourth of the drop volume was saturated with [Co(NH3)5Cl]Cl2 solution, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.35 Å R-free 0.239 |
| 2I7S Crystal structure of Re(phen)(CO)3 (Thr124His)(His83Gln) Azurin Cu(II) from Pseudomonas aeruginosa Deposited 2006-08-31 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
21–148（128 aa）
Chain D
21–148（128 aa）
|
Mutation：H83Q, T124H Mutation：H83Q, T124H | CU COPPER (II) ION × 2 REP (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 2 CON COBALT TETRAAMMINE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG4000, 100 mM LiNO3, 100 mM Imidazole pH 7.0. One fourth of the drop volume was saturated with [Co(NH3)5Cl]Cl2 solution, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
|
Resolution 1.35 Å R-free 0.239 |
| 2IDF P. aeruginosa azurin N42C/M64E double mutant, BMME-linked dimer Deposited 2006-09-15 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
|
Mutation：N42C, M64E Mutation：N42C, M64E | CU COPPER (II) ION × 2 NI NICKEL (II) ION × 2 144 TRIS-HYDROXYMETHYL-METHYL-AMMONIUM × 2 OPP 1-[PYRROL-1-YL-2,5-DIONE-METHOXYMETHYL]-PYRROLE-2,5-DIONE × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 9；293 K；25% PEG 2000 MME, 10 mM NiCl2, 100 mM Tris/HCl, pH 9.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.25 Å R-free 0.261 |
| 2IWE Structure of a cavity mutant (H117G) of Pseudomonas aeruginosa azurin Deposited 2006-06-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
Chain J
21–148（128 aa）
|
Mutation：YES Mutation：YES | ZN ZINC ION × 2 2IH 1,1'-HEXANE-1,6-DIYLBIS(1H-IMIDAZOLE) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.5；100 MM TRIS-HCL PH 8.5 AND 20% (W/V) POLYETHYLENE GLYCOL (PEG) 8000
|
Resolution 2.83 Å R-free 0.233 |
| 2IWE Structure of a cavity mutant (H117G) of Pseudomonas aeruginosa azurin Deposited 2006-06-29 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain D
21–148（128 aa）
Chain G
21–148（128 aa）
|
Mutation：YES Mutation：YES | ZN ZINC ION × 2 2IH 1,1'-HEXANE-1,6-DIYLBIS(1H-IMIDAZOLE) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 8.5；100 MM TRIS-HCL PH 8.5 AND 20% (W/V) POLYETHYLENE GLYCOL (PEG) 8000
|
Resolution 2.83 Å R-free 0.233 |
| 2OJ1 Disulfide-linked dimer of azurin N42C/M64E double mutant Deposited 2007-01-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：N42C, M64E | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 3.1；293 K；30 % PEG 4000, 0.1M sodium citrate buffer, pH 3.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.281 |
| 2OJ1 Disulfide-linked dimer of azurin N42C/M64E double mutant Deposited 2007-01-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：N42C, M64E | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 3.1；293 K；30 % PEG 4000, 0.1M sodium citrate buffer, pH 3.1, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.281 |
| 2TSA AZURIN MUTANT M121A Deposited 1996-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
Chain C
21–148（128 aa）
Chain D
21–148（128 aa）
|
Mutation：M121A Mutation：M121A Mutation：M121A Mutation：M121A | CU COPPER (II) ION × 4 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.20 Å |
| 2TSB AZURIN MUTANT M121A-AZIDE Deposited 1996-05-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
Chain C
21–148（128 aa）
Chain D
21–148（128 aa）
|
Mutation：M121A Mutation：M121A Mutation：M121A Mutation：M121A | AZI AZIDE ION × 4 CU COPPER (II) ION × 4 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.30 Å |
| 2XV0 Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAHAAM), chemically reduced, pH4.8 Deposited 2010-10-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：YES | CU1 COPPER (I) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.8；293 K；0.1M POTASSIUM THIOCYANATE, 30% PEG2000, PH 7.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 1.60 Å R-free 0.199 |
| 2XV2 Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAHAAM), chemically reduced, pH4.2 Deposited 2010-10-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：YES | CU1 COPPER (I) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 4.2；293 K；0.1M POTASSIUM THIOCYANATE, 30% PEG2000, PH 7.0, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 1.60 Å R-free 0.200 |
| 2XV3 Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced, pH5.3 Deposited 2010-10-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：YES | CU1 COPPER (I) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.3；293 K；10MM TRI-SODIUM CITRATE, 33% PEG6000, PH 7.6, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.30 Å R-free 0.266 |
| 2XV3 Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced, pH5.3 Deposited 2010-10-22 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：YES | CU1 COPPER (I) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.3；293 K；10MM TRI-SODIUM CITRATE, 33% PEG6000, PH 7.6, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K
|
Resolution 2.30 Å R-free 0.266 |
| 3AZU X-RAY CRYSTAL STRUCTURE OF THE TWO SITE-SPECIFIC MUTANTS HIS35GLN AND HIS35LEU OF AZURIN FROM PSEUDOMONAS AERUGINOSA Deposited 1991-01-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
Chain C
21–148（128 aa）
Chain D
21–148（128 aa）
|
Mutation：H35Q Mutation：H35Q Mutation：H35Q Mutation：H35Q | CU COPPER (II) ION × 4 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 2.10 Å |
| 3FPY Azurin C112D/M121L Deposited 2009-01-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：C112D, M121L | CU COPPER (II) ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；25% PEG 4000, 0.1 M sodium acetate pH 5.6, 0.1 M tris pH 8, 0.1 M lithium nitrate, 0.01 M copper(II) sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.10 Å R-free 0.236 |
| 3FQ1 Azurin C112D/M121I Deposited 2009-01-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：C112D, M121I | CU COPPER (II) ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；25% PEG 4000, 0.1M sodium acetate pH 5.6, 0.1 M tris pH 8, 0.1 M lithium nitrate, 0.01 M copper(II) sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.256 |
| 3FQ2 Azurin C112D/M121F Deposited 2009-01-06 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：C112D, M121F | CU COPPER (II) ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；25% PEG 4000, 0.1 M sodium acetate pH 5.6, 0.1 M tris pH 8, 0.1 M lithium nitrate, 0.01 M copper(II) sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.91 Å R-free 0.245 |
| 3FQY Azurin C112D Deposited 2009-01-07 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：C112D | CU COPPER (II) ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；25% PEG 4000, 0.1M sodium acetate pH 5.6, 0.1 M tris pH 8, 0.1 M lithium nitrate, 0.01 M copper(II) sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.90 Å R-free 0.244 |
| 3FS9 Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAHAAM) Deposited 2009-01-09 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.1M potassium thiocyanate, 30% PEG 2000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.05 Å R-free 0.145 |
| 3FSA Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAHAAM); chemically reduced. Deposited 2009-01-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU1 COPPER (I) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；0.1M potassium thiocyanate, 30% PEG 2000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 0.98 Å R-free 0.135 |
| 3FSV Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAHAAAM) Deposited 2009-01-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；150mM potassium bromide, 30% PEG MME 2000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.308 |
| 3FSV Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAHAAAM) Deposited 2009-01-12 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；293 K；150mM potassium bromide, 30% PEG MME 2000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.30 Å R-free 0.308 |
| 3FSW Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAM) Deposited 2009-01-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100mM Hepes pH 8.0, 34% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.256 |
| 3FSW Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAM) Deposited 2009-01-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100mM Hepes pH 8.0, 34% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.256 |
| 3FSW Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAM) Deposited 2009-01-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100mM Hepes pH 8.0, 34% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.256 |
| 3FSW Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAM) Deposited 2009-01-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；293 K；100mM Hepes pH 8.0, 34% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.256 |
| 3FSZ Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM) Deposited 2009-01-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；293 K；10mM tri-sodium citrate, 33% PEG 6000, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.255 |
| 3FSZ Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM) Deposited 2009-01-12 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；293 K；10mM tri-sodium citrate, 33% PEG 6000, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 2.00 Å R-free 0.255 |
| 3FT0 Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced Deposited 2009-01-12 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU1 COPPER (I) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；293 K；10mM tri-sodium citrate, 33% PEG 6000, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.250 |
| 3FT0 Pseudomonas aeruginosa Azurin with mutated metal-binding loop sequence (CAAAAHAAAAM), chemically reduced Deposited 2009-01-12 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Not recorded | CU1 COPPER (I) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.6；293 K；10mM tri-sodium citrate, 33% PEG 6000, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K
|
Resolution 1.80 Å R-free 0.250 |
| 3IBO Pseudomonas aeruginosa E2Q/H83Q/T126H-azurin RE(PHEN)(CO)3 Deposited 2009-07-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：E2Q, H83Q, T126H | CU COPPER (II) ION × 1 REP (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG4000, 100 MM LINO3, 100 MM IMIDAZOLE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.45 Å R-free 0.217 |
| 3IBO Pseudomonas aeruginosa E2Q/H83Q/T126H-azurin RE(PHEN)(CO)3 Deposited 2009-07-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：E2Q, H83Q, T126H | CU COPPER (II) ION × 1 REP (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG4000, 100 MM LINO3, 100 MM IMIDAZOLE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.45 Å R-free 0.217 |
| 3IBO Pseudomonas aeruginosa E2Q/H83Q/T126H-azurin RE(PHEN)(CO)3 Deposited 2009-07-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
|
Mutation：E2Q, H83Q, T126H | CU COPPER (II) ION × 1 REP (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG4000, 100 MM LINO3, 100 MM IMIDAZOLE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.45 Å R-free 0.217 |
| 3IBO Pseudomonas aeruginosa E2Q/H83Q/T126H-azurin RE(PHEN)(CO)3 Deposited 2009-07-16 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
|
Mutation：E2Q, H83Q, T126H | CU COPPER (II) ION × 1 REP (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；20% PEG4000, 100 MM LINO3, 100 MM IMIDAZOLE, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K
|
Resolution 1.45 Å R-free 0.217 |
| 3IN0 Crystal structure of the F114P/M121Q variant of Pseudomonas aeruginosa azurin in the Cu(II) state Deposited 2009-08-11 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：F114P, M121Q | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；PEG 8000, 0.08M NaOAc, 0.2M Lithium nitrate, 0.2M Calcium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.35 Å R-free 0.286 |
| 3IN0 Crystal structure of the F114P/M121Q variant of Pseudomonas aeruginosa azurin in the Cu(II) state Deposited 2009-08-11 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：F114P, M121Q | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；PEG 8000, 0.08M NaOAc, 0.2M Lithium nitrate, 0.2M Calcium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.35 Å R-free 0.286 |
| 3IN0 Crystal structure of the F114P/M121Q variant of Pseudomonas aeruginosa azurin in the Cu(II) state Deposited 2009-08-11 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
|
Mutation：F114P, M121Q | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；PEG 8000, 0.08M NaOAc, 0.2M Lithium nitrate, 0.2M Calcium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.35 Å R-free 0.286 |
| 3IN0 Crystal structure of the F114P/M121Q variant of Pseudomonas aeruginosa azurin in the Cu(II) state Deposited 2009-08-11 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
|
Mutation：F114P, M121Q | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；PEG 8000, 0.08M NaOAc, 0.2M Lithium nitrate, 0.2M Calcium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.35 Å R-free 0.286 |
| 3IN2 Crystal structure of the N47S/M121L variant of Pseudomonas aeruginosa azurin in the Cu(II) state Deposited 2009-08-11 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：N47S, M121L | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；PEG 8000, 0.08M NaOAc, 0.2M Lithium nitrate, 0.2M Calcium chloride, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.60 Å R-free 0.266 |
| 3JT2 Cu(II) N47S/M121L variant of Pseudomonas Aeruginosa azurin Deposited 2009-09-11 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：N47S, M121L | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；80mM sodium acetate, 0.24M calcium chloride, 0.24M lithium nitrate, 25% PEG 8000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.10 Å R-free 0.288 |
| 3JT2 Cu(II) N47S/M121L variant of Pseudomonas Aeruginosa azurin Deposited 2009-09-11 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：N47S, M121L | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；80mM sodium acetate, 0.24M calcium chloride, 0.24M lithium nitrate, 25% PEG 8000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 2.10 Å R-free 0.288 |
| 3JTB Cu(II) N47S/F114N variant of Pseudomonas Aeruginosa Azurin Deposited 2009-09-11 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：N47S, F114N | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；80mM sodium acetate, 0.24M calcium chloride, 0.24M lithium nitrate, 25% PEG 8000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.259 |
| 3JTB Cu(II) N47S/F114N variant of Pseudomonas Aeruginosa Azurin Deposited 2009-09-11 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：N47S, F114N | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；80mM sodium acetate, 0.24M calcium chloride, 0.24M lithium nitrate, 25% PEG 8000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.259 |
| 3JTB Cu(II) N47S/F114N variant of Pseudomonas Aeruginosa Azurin Deposited 2009-09-11 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
|
Mutation：N47S, F114N | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；80mM sodium acetate, 0.24M calcium chloride, 0.24M lithium nitrate, 25% PEG 8000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.259 |
| 3JTB Cu(II) N47S/F114N variant of Pseudomonas Aeruginosa Azurin Deposited 2009-09-11 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
|
Mutation：N47S, F114N | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6；277 K；80mM sodium acetate, 0.24M calcium chloride, 0.24M lithium nitrate, 25% PEG 8000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.80 Å R-free 0.259 |
| 3N2J Azurin H117G, oxidized form Deposited 2010-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.7；293 K；31 % PEG 2000 MME
0.1 M Tris/HCl pH 8.7
, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.35 Å R-free 0.202 |
| 3N2J Azurin H117G, oxidized form Deposited 2010-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 10 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain J
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.7；293 K；31 % PEG 2000 MME
0.1 M Tris/HCl pH 8.7
, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.35 Å R-free 0.202 |
| 3N2J Azurin H117G, oxidized form Deposited 2010-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 11 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain K
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.7；293 K；31 % PEG 2000 MME
0.1 M Tris/HCl pH 8.7
, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.35 Å R-free 0.202 |
| 3N2J Azurin H117G, oxidized form Deposited 2010-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 12 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain L
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.7；293 K；31 % PEG 2000 MME
0.1 M Tris/HCl pH 8.7
, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.35 Å R-free 0.202 |
| 3N2J Azurin H117G, oxidized form Deposited 2010-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.7；293 K；31 % PEG 2000 MME
0.1 M Tris/HCl pH 8.7
, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.35 Å R-free 0.202 |
| 3N2J Azurin H117G, oxidized form Deposited 2010-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.7；293 K；31 % PEG 2000 MME
0.1 M Tris/HCl pH 8.7
, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.35 Å R-free 0.202 |
| 3N2J Azurin H117G, oxidized form Deposited 2010-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.7；293 K；31 % PEG 2000 MME
0.1 M Tris/HCl pH 8.7
, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.35 Å R-free 0.202 |
| 3N2J Azurin H117G, oxidized form Deposited 2010-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.7；293 K；31 % PEG 2000 MME
0.1 M Tris/HCl pH 8.7
, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.35 Å R-free 0.202 |
| 3N2J Azurin H117G, oxidized form Deposited 2010-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.7；293 K；31 % PEG 2000 MME
0.1 M Tris/HCl pH 8.7
, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.35 Å R-free 0.202 |
| 3N2J Azurin H117G, oxidized form Deposited 2010-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 7 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain G
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.7；293 K；31 % PEG 2000 MME
0.1 M Tris/HCl pH 8.7
, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.35 Å R-free 0.202 |
| 3N2J Azurin H117G, oxidized form Deposited 2010-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 8 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain H
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.7；293 K；31 % PEG 2000 MME
0.1 M Tris/HCl pH 8.7
, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.35 Å R-free 0.202 |
| 3N2J Azurin H117G, oxidized form Deposited 2010-05-18 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 9 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain I
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8.7；293 K；31 % PEG 2000 MME
0.1 M Tris/HCl pH 8.7
, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.35 Å R-free 0.202 |
| 3NP3 C112D/M121E Pseudomonas Aeruginosa Azurin Deposited 2010-06-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：C112D, M121E | CU COPPER (II) ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；25-30% PEG 4000
100 mM LiNO3
20 mM CuCl2
100 mM Tris pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.10 Å R-free 0.265 |
| 3NP4 C112D/M121E Pseudomonas aeruginosa Azurin Deposited 2010-06-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：C112D, M121E | CU COPPER (II) ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；25-30% PEG 4000
100 mM LiNO3
20 mM CuCl2
100 mM Tris pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.25 Å R-free 0.276 |
| 3OQR C112D/M121E Azurin, pH 10.0 Deposited 2010-09-03 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：C112D, M121E | CU COPPER (II) ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7；298 K；25% PEG 4000, 0.1 M sodium acetate pH 5.6, 0.1 M tris pH 8, 0.1 M lithium nitrate, 0.01 M copper(II) chloride, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 2.40 Å R-free 0.331 |
| 3U25 Crystal structure of P. aeruginoas azurin containing a Tyr-His hydrogen bonded pair Deposited 2011-09-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–148（127 aa）
Fragment：unp residues 22-148
|
Mutation：H40I, Y48W, F72Y, F108Y | CU COPPER (II) ION × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；25-30% of poly(ethylene glycol) (PEG) 4000, 100 mM lithium nitrate, 10 mM copper sulfate and 100 mM Tris HCl, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.18 Å R-free 0.217 |
| 3U25 Crystal structure of P. aeruginoas azurin containing a Tyr-His hydrogen bonded pair Deposited 2011-09-30 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–148（127 aa）
Fragment：unp residues 22-148
|
Mutation：H40I, Y48W, F72Y, F108Y | CU COPPER (II) ION × 1 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；298 K；25-30% of poly(ethylene glycol) (PEG) 4000, 100 mM lithium nitrate, 10 mM copper sulfate and 100 mM Tris HCl, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.18 Å R-free 0.217 |
| 3UGE Silver Metallated Pseudomonas aeruginosa Azurin at 1.70 A Deposited 2011-11-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
Fragment：UNP residues 21-148
|
Not recorded | AG SILVER ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；3.2 M ammonium sulfate, 0.5 M lithium nitrate, 50 mM sodium acetate, pH 5.5, soaked with 1 mM silver nitrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.70 Å R-free 0.245 |
| 3UGE Silver Metallated Pseudomonas aeruginosa Azurin at 1.70 A Deposited 2011-11-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
Fragment：UNP residues 21-148
|
Not recorded | AG SILVER ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；3.2 M ammonium sulfate, 0.5 M lithium nitrate, 50 mM sodium acetate, pH 5.5, soaked with 1 mM silver nitrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.70 Å R-free 0.245 |
| 3UGE Silver Metallated Pseudomonas aeruginosa Azurin at 1.70 A Deposited 2011-11-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
Fragment：UNP residues 21-148
|
Not recorded | AG SILVER ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；3.2 M ammonium sulfate, 0.5 M lithium nitrate, 50 mM sodium acetate, pH 5.5, soaked with 1 mM silver nitrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.70 Å R-free 0.245 |
| 3UGE Silver Metallated Pseudomonas aeruginosa Azurin at 1.70 A Deposited 2011-11-02 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
Fragment：UNP residues 21-148
|
Not recorded | AG SILVER ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 5.5；298 K；3.2 M ammonium sulfate, 0.5 M lithium nitrate, 50 mM sodium acetate, pH 5.5, soaked with 1 mM silver nitrate, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.70 Å R-free 0.245 |
| 4AZU CRYSTAL STRUCTURE ANALYSIS OF OXIDIZED PSEUDOMONAS AERUGINOSA AZURIN AT PH 5.5 AND PH 9.0. A PH-INDUCED CONFORMATIONAL TRANSITION INVOLVES A PEPTIDE BOND FLIP Deposited 1993-06-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
Chain C
21–148（128 aa）
Chain D
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 4 NO3 NITRATE ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 4BWW Crystal structure of spin labelled azurin T21R1. Deposited 2013-07-04 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
Chain C
21–148（128 aa）
Chain D
21–148（128 aa）
|
Mutation：YES Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：YES Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：YES Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：YES Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 4 GOL GLYCEROL × 8 NO3 NITRATE ION × 5 SO4 SULFATE ION × 5 |
X-RAY DIFFRACTION
X-ray crystallization conditions
2.14 M AMMONIUM SULFATE, 0.28 M AMMONIUM NITRATE, 0.1 M SODIUM CACODYLATE PH 6.0
|
Resolution 1.48 Å R-free 0.210 |
| 4HHG Crystal structure of the Pseudomonas aeruginosa azurin, RuH107NO YOH109 Deposited 2012-10-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：W68F,Y92F,H103Q,Y128F,M129NIY,Q127H Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 DRU DELTA-BIS(2,2'-BIPYRIDINE)IMIDAZOLE RUTHENIUM (II) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293.15 K；26-34% PEG 4000, 100mM Lithium Nitrate, 6.25mM Copper Sulfate and 100 mM Imidazole, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
Resolution 1.60 Å R-free 0.261 |
| 4HHG Crystal structure of the Pseudomonas aeruginosa azurin, RuH107NO YOH109 Deposited 2012-10-09 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
|
Mutation：W68F,Y92F,H103Q,Y128F,M129NIY,Q127H Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 2 DRU DELTA-BIS(2,2'-BIPYRIDINE)IMIDAZOLE RUTHENIUM (II) × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293.15 K；26-34% PEG 4000, 100mM Lithium Nitrate, 6.25mM Copper Sulfate and 100 mM Imidazole, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
Resolution 1.60 Å R-free 0.261 |
| 4HHW Crystal structure of the Pseudomonas aeruginosa azurin, H124NO YOH122 Deposited 2012-10-10 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：W68F,Y92F,H103Q,Y128F,K142NIY,T144H Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293.15 K；26-34% PEG 4000, 100 mM Lithium Nitrate, 6.25 mM Copper Sulfate and 100 mM Imidazole, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
Resolution 2.00 Å R-free 0.281 |
| 4HHW Crystal structure of the Pseudomonas aeruginosa azurin, H124NO YOH122 Deposited 2012-10-10 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：W68F,Y92F,H103Q,Y128F,K142NIY,T144H Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293.15 K；26-34% PEG 4000, 100 mM Lithium Nitrate, 6.25 mM Copper Sulfate and 100 mM Imidazole, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
Resolution 2.00 Å R-free 0.281 |
| 4HHW Crystal structure of the Pseudomonas aeruginosa azurin, H124NO YOH122 Deposited 2012-10-10 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
|
Mutation：W68F,Y92F,H103Q,Y128F,K142NIY,T144H Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293.15 K；26-34% PEG 4000, 100 mM Lithium Nitrate, 6.25 mM Copper Sulfate and 100 mM Imidazole, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
Resolution 2.00 Å R-free 0.281 |
| 4HHW Crystal structure of the Pseudomonas aeruginosa azurin, H124NO YOH122 Deposited 2012-10-10 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
21–148（128 aa）
|
Mutation：W68F,Y92F,H103Q,Y128F,K142NIY,T144H Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293.15 K；26-34% PEG 4000, 100 mM Lithium Nitrate, 6.25 mM Copper Sulfate and 100 mM Imidazole, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
Resolution 2.00 Å R-free 0.281 |
| 4HIP Crystal structure of the Pseudomonas aeruginosa azurin, H126NO YOH109 Deposited 2012-10-11 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：W68F,Y92F,H103Q,Y128F,K142NIY,T146H Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293.15 K；26-34% PEG 4000, 100 mM Lithium Nitrate, 6.25 mM Copper Sulfate and 100 mM Imidazole, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
Resolution 1.90 Å R-free 0.317 |
| 4HIP Crystal structure of the Pseudomonas aeruginosa azurin, H126NO YOH109 Deposited 2012-10-11 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：W68F,Y92F,H103Q,Y128F,K142NIY,T146H Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293.15 K；26-34% PEG 4000, 100 mM Lithium Nitrate, 6.25 mM Copper Sulfate and 100 mM Imidazole, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
Resolution 1.90 Å R-free 0.317 |
| 4HIP Crystal structure of the Pseudomonas aeruginosa azurin, H126NO YOH109 Deposited 2012-10-11 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
|
Mutation：W68F,Y92F,H103Q,Y128F,K142NIY,T146H Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293.15 K；26-34% PEG 4000, 100 mM Lithium Nitrate, 6.25 mM Copper Sulfate and 100 mM Imidazole, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
Resolution 1.90 Å R-free 0.317 |
| 4HIP Crystal structure of the Pseudomonas aeruginosa azurin, H126NO YOH109 Deposited 2012-10-11 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain B
21–148（128 aa）
|
Mutation：W68F,Y92F,H103Q,Y128F,K142NIY,T146H Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.2；293.15 K；26-34% PEG 4000, 100 mM Lithium Nitrate, 6.25 mM Copper Sulfate and 100 mM Imidazole, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K
|
Resolution 1.90 Å R-free 0.317 |
| 4HZ1 Crystal Structure of Pseudomonas aeruginosa azurin with iron(II) at the copper-binding site. Deposited 2012-11-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | ACT ACETATE ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；300 K；3.0 - 3.5 M ammonium sulfate, 0.5 M lithium nitrate, 0.1 M sodium acetate pH 5.2-5.4, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 2.20 Å R-free 0.277 |
| 4HZ1 Crystal Structure of Pseudomonas aeruginosa azurin with iron(II) at the copper-binding site. Deposited 2012-11-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Not recorded | ACT ACETATE ION × 1 FE2 FE (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；300 K；3.0 - 3.5 M ammonium sulfate, 0.5 M lithium nitrate, 0.1 M sodium acetate pH 5.2-5.4, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 2.20 Å R-free 0.277 |
| 4HZ1 Crystal Structure of Pseudomonas aeruginosa azurin with iron(II) at the copper-binding site. Deposited 2012-11-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
|
Not recorded | ACT ACETATE ION × 1 FE2 FE (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；300 K；3.0 - 3.5 M ammonium sulfate, 0.5 M lithium nitrate, 0.1 M sodium acetate pH 5.2-5.4, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 2.20 Å R-free 0.277 |
| 4HZ1 Crystal Structure of Pseudomonas aeruginosa azurin with iron(II) at the copper-binding site. Deposited 2012-11-14 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
|
Not recorded | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；300 K；3.0 - 3.5 M ammonium sulfate, 0.5 M lithium nitrate, 0.1 M sodium acetate pH 5.2-5.4, VAPOR DIFFUSION, HANGING DROP, temperature 300K
|
Resolution 2.20 Å R-free 0.277 |
| 4JKN Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A Deposited 2013-03-10 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
Fragment：UNP residues 21-148
|
Not recorded | HG MERCURY (II) ION × 2 NO3 NITRATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.5；298 K；3.2 M ammonium sulfate, 0.5 M lithium nitrate, 50 mM sodium acetate, pH 5.5, soaked with 3 fold excess Mercury Chloride , VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.54 Å R-free 0.239 |
| 4JKN Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A Deposited 2013-03-10 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
Fragment：UNP residues 21-148
|
Not recorded | HG MERCURY (II) ION × 1 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.5；298 K；3.2 M ammonium sulfate, 0.5 M lithium nitrate, 50 mM sodium acetate, pH 5.5, soaked with 3 fold excess Mercury Chloride , VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.54 Å R-free 0.239 |
| 4JKN Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A Deposited 2013-03-10 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
Fragment：UNP residues 21-148
|
Not recorded | HG MERCURY (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.5；298 K；3.2 M ammonium sulfate, 0.5 M lithium nitrate, 50 mM sodium acetate, pH 5.5, soaked with 3 fold excess Mercury Chloride , VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.54 Å R-free 0.239 |
| 4JKN Mercury Metallated Pseudomonas aeruginosa Azurin at 1.54 A Deposited 2013-03-10 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
Fragment：UNP residues 21-148
|
Not recorded | HG MERCURY (II) ION × 1 NO3 NITRATE ION × 4 SO4 SULFATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 5.5；298 K；3.2 M ammonium sulfate, 0.5 M lithium nitrate, 50 mM sodium acetate, pH 5.5, soaked with 3 fold excess Mercury Chloride , VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.54 Å R-free 0.239 |
| 4K9J Structure of Re(CO)3(4,7-dimethyl-phen)(Thr126His)(Lys122Trp)(His83Glu)(Trp48Phe)(Tyr72Phe)(Tyr108Phe)AzCu(II), a Rhenium modified Azurin mutant Deposited 2013-04-20 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：t126H, k122w, h83e,w48f, y72f | CU COPPER (II) ION × 1 REQ (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 7.4；298 K；protein buffer:40 mM imidazole, 2 mM NaCl. Reservoir: 100 mM imidazole, 100 mM LiNO3, 6.25 mM CuCl2, 27% PEG 4000, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 298K
|
Resolution 1.70 Å R-free 0.236 |
| 4KO5 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/W48L/V95I/Y108F) Deposited 2013-05-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V31I/W48L/V95I/Y108F | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.79 Å R-free 0.240 |
| 4KO5 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/W48L/V95I/Y108F) Deposited 2013-05-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V31I/W48L/V95I/Y108F | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.79 Å R-free 0.240 |
| 4KO6 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/V95K/Y108F) Deposited 2013-05-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V31I/V95K/Y108F | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.74 Å R-free 0.273 |
| 4KO6 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/V95K/Y108F) Deposited 2013-05-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V31I/V95K/Y108F | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.74 Å R-free 0.273 |
| 4KO6 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/V95K/Y108F) Deposited 2013-05-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V31I/V95K/Y108F | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.74 Å R-free 0.273 |
| 4KO6 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/V95K/Y108F) Deposited 2013-05-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V31I/V95K/Y108F | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.74 Å R-free 0.273 |
| 4KO7 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/W48F/V95I) Deposited 2013-05-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V31I/W48F/V95I | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.07 Å R-free 0.266 |
| 4KO7 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/W48F/V95I) Deposited 2013-05-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V31I/W48F/V95I | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.07 Å R-free 0.266 |
| 4KO7 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/W48F/V95I) Deposited 2013-05-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V31I/W48F/V95I | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.07 Å R-free 0.266 |
| 4KO7 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/W48F/V95I) Deposited 2013-05-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V31I/W48F/V95I | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.07 Å R-free 0.266 |
| 4KO9 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V95I/Y108F) Deposited 2013-05-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V95I/Y108F | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.05 Å R-free 0.226 |
| 4KO9 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V95I/Y108F) Deposited 2013-05-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V95I/Y108F | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.05 Å R-free 0.226 |
| 4KO9 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V95I/Y108F) Deposited 2013-05-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V95I/Y108F | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.05 Å R-free 0.226 |
| 4KO9 Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V95I/Y108F) Deposited 2013-05-11 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V95I/Y108F | CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 2.05 Å R-free 0.226 |
| 4KOB Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/V95I) Deposited 2013-05-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V31I/V95I | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.87 Å R-free 0.226 |
| 4KOB Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/V95I) Deposited 2013-05-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V31I/V95I | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.87 Å R-free 0.226 |
| 4KOB Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/V95I) Deposited 2013-05-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V31I/V95I | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.87 Å R-free 0.226 |
| 4KOB Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/V95I) Deposited 2013-05-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V31I/V95I | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.87 Å R-free 0.226 |
| 4KOC Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V31I/V95I/Y108F) Deposited 2013-05-11 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：V31I/V95I/Y108F | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；100 mM Tris-HCl, 100 mM lithium nitrate, 10 mM copper sulfate, 30% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
|
Resolution 1.46 Å R-free 0.238 |
| 4MFH Crystal Structure of M121G Azurin Deposited 2013-08-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：M121G | CU COPPER (II) ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；2 UL droplet containing 1 UL apo-M121G azurin (1.3 mM) in 100 mM NaOAc pH 5.6 buffer and 1 UL PEG buffer (25% PEG 4000 containing 100 mM LiNO3, 10 mM CuSO4 and 100 mM Tris pH 8.0) above 250 UL well buffer (25% PEG 4000 containing 100 mM LiNO3 10 mM CuSO4 and 100 mM Tris pH 8.0), VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.54 Å R-free 0.194 |
| 4MFH Crystal Structure of M121G Azurin Deposited 2013-08-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：M121G | CU COPPER (II) ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；2 UL droplet containing 1 UL apo-M121G azurin (1.3 mM) in 100 mM NaOAc pH 5.6 buffer and 1 UL PEG buffer (25% PEG 4000 containing 100 mM LiNO3, 10 mM CuSO4 and 100 mM Tris pH 8.0) above 250 UL well buffer (25% PEG 4000 containing 100 mM LiNO3 10 mM CuSO4 and 100 mM Tris pH 8.0), VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.54 Å R-free 0.194 |
| 4MFH Crystal Structure of M121G Azurin Deposited 2013-08-27 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
|
Mutation：M121G | CU COPPER (II) ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；2 UL droplet containing 1 UL apo-M121G azurin (1.3 mM) in 100 mM NaOAc pH 5.6 buffer and 1 UL PEG buffer (25% PEG 4000 containing 100 mM LiNO3, 10 mM CuSO4 and 100 mM Tris pH 8.0) above 250 UL well buffer (25% PEG 4000 containing 100 mM LiNO3 10 mM CuSO4 and 100 mM Tris pH 8.0), VAPOR DIFFUSION, HANGING DROP, temperature 277K
|
Resolution 1.54 Å R-free 0.194 |
| 4QKT Azurin mutant M121EM44K with copper Deposited 2014-06-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：M121E, M44K | CU COPPER (II) ION × 3 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；278 K；pH8.0 100mM LiNO3 100 mM Tris 25%PEG4000 1:1 mixing-medium, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 1.64 Å R-free 0.216 |
| 4QKT Azurin mutant M121EM44K with copper Deposited 2014-06-09 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：M121E, M44K | CU COPPER (II) ION × 3 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 ACT ACETATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；278 K；pH8.0 100mM LiNO3 100 mM Tris 25%PEG4000 1:1 mixing-medium, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 1.64 Å R-free 0.216 |
| 4QLW Azurin mutant M121E with iron Deposited 2014-06-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：M121E | SO4 SULFATE ION × 2 NO3 NITRATE ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.3；278 K；(NH4)2SO4 (3.25 M), LiNO3 (0.1 M), NaOAc (0.1 M), pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 2.00 Å R-free 0.262 |
| 4QLW Azurin mutant M121E with iron Deposited 2014-06-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：M121E | NO3 NITRATE ION × 2 FE FE (III) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.3；278 K；(NH4)2SO4 (3.25 M), LiNO3 (0.1 M), NaOAc (0.1 M), pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 2.00 Å R-free 0.262 |
| 4QLW Azurin mutant M121E with iron Deposited 2014-06-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
|
Mutation：M121E | SO4 SULFATE ION × 1 NO3 NITRATE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.3；278 K；(NH4)2SO4 (3.25 M), LiNO3 (0.1 M), NaOAc (0.1 M), pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 2.00 Å R-free 0.262 |
| 4QLW Azurin mutant M121E with iron Deposited 2014-06-13 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
|
Mutation：M121E | NO3 NITRATE ION × 2 FE FE (III) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.3；278 K；(NH4)2SO4 (3.25 M), LiNO3 (0.1 M), NaOAc (0.1 M), pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 2.00 Å R-free 0.262 |
| 4QLW Azurin mutant M121E with iron Deposited 2014-06-13 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 5 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
Chain C
21–148（128 aa）
Chain D
21–148（128 aa）
|
Mutation：M121E Mutation：M121E Mutation：M121E Mutation：M121E | SO4 SULFATE ION × 3 NO3 NITRATE ION × 10 FE FE (III) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.3；278 K；(NH4)2SO4 (3.25 M), LiNO3 (0.1 M), NaOAc (0.1 M), pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 278K
|
Resolution 2.00 Å R-free 0.262 |
| 4WKX Reversible S-Nitrosylation in an Engineered Mutant of Pseudomonas aeruginosa Azurin with Red Copper Site Deposited 2014-10-03 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Mutation：H66E, M141H | CU COPPER (II) ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；25% PEG 4000, 100 mM LiNO3, 10 mM CuSO4 and 100 mM Tris
|
Resolution 1.94 Å R-free 0.235 |
| 4WKX Reversible S-Nitrosylation in an Engineered Mutant of Pseudomonas aeruginosa Azurin with Red Copper Site Deposited 2014-10-03 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Mutation：H66E, M141H | CU COPPER (II) ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；25% PEG 4000, 100 mM LiNO3, 10 mM CuSO4 and 100 mM Tris
|
Resolution 1.94 Å R-free 0.235 |
| 4WKX Reversible S-Nitrosylation in an Engineered Mutant of Pseudomonas aeruginosa Azurin with Red Copper Site Deposited 2014-10-03 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
|
Mutation：H66E, M141H Mutation：H66E, M141H | CU COPPER (II) ION × 4 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；25% PEG 4000, 100 mM LiNO3, 10 mM CuSO4 and 100 mM Tris
|
Resolution 1.94 Å R-free 0.235 |
| 5AZU CRYSTAL STRUCTURE ANALYSIS OF OXIDIZED PSEUDOMONAS AERUGINOSA AZURIN AT PH 5.5 AND PH 9.0. A PH-INDUCED CONFORMATIONAL TRANSITION INVOLVES A PEPTIDE BOND FLIP Deposited 1993-06-23 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
Chain C
21–148（128 aa）
Chain D
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 4 NO3 NITRATE ION × 1 | X-RAY DIFFRACTION mmCIF provides none of the parsed conditions | Resolution 1.90 Å |
| 5I26 Azurin T30R1, crystal form I Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；Sodium malonate
|
Resolution 1.89 Å R-free 0.240 |
| 5I26 Azurin T30R1, crystal form I Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；Sodium malonate
|
Resolution 1.89 Å R-free 0.240 |
| 5I26 Azurin T30R1, crystal form I Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；Sodium malonate
|
Resolution 1.89 Å R-free 0.240 |
| 5I26 Azurin T30R1, crystal form I Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；Sodium malonate
|
Resolution 1.89 Å R-free 0.240 |
| 5I28 Azurin T30R1, crystal form II Deposited 2016-02-08 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 GOL GLYCEROL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；sodium malonate
|
Resolution 1.95 Å R-free 0.271 |
| 5I28 Azurin T30R1, crystal form II Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 10 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain J
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；sodium malonate
|
Resolution 1.95 Å R-free 0.271 |
| 5I28 Azurin T30R1, crystal form II Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 11 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain K
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；sodium malonate
|
Resolution 1.95 Å R-free 0.271 |
| 5I28 Azurin T30R1, crystal form II Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 12 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain L
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；sodium malonate
|
Resolution 1.95 Å R-free 0.271 |
| 5I28 Azurin T30R1, crystal form II Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 13 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain M
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；sodium malonate
|
Resolution 1.95 Å R-free 0.271 |
| 5I28 Azurin T30R1, crystal form II Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 14 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain N
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；sodium malonate
|
Resolution 1.95 Å R-free 0.271 |
| 5I28 Azurin T30R1, crystal form II Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 15 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain O
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；sodium malonate
|
Resolution 1.95 Å R-free 0.271 |
| 5I28 Azurin T30R1, crystal form II Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 16 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain P
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；sodium malonate
|
Resolution 1.95 Å R-free 0.271 |
| 5I28 Azurin T30R1, crystal form II Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；sodium malonate
|
Resolution 1.95 Å R-free 0.271 |
| 5I28 Azurin T30R1, crystal form II Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；sodium malonate
|
Resolution 1.95 Å R-free 0.271 |
| 5I28 Azurin T30R1, crystal form II Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；sodium malonate
|
Resolution 1.95 Å R-free 0.271 |
| 5I28 Azurin T30R1, crystal form II Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 5 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain E
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；sodium malonate
|
Resolution 1.95 Å R-free 0.271 |
| 5I28 Azurin T30R1, crystal form II Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 6 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain F
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；sodium malonate
|
Resolution 1.95 Å R-free 0.271 |
| 5I28 Azurin T30R1, crystal form II Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 7 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain G
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；sodium malonate
|
Resolution 1.95 Å R-free 0.271 |
| 5I28 Azurin T30R1, crystal form II Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 8 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain H
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；sodium malonate
|
Resolution 1.95 Å R-free 0.271 |
| 5I28 Azurin T30R1, crystal form II Deposited 2016-02-08 | Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 9 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain I
21–148（128 aa）
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；298 K；sodium malonate
|
Resolution 1.95 Å R-free 0.271 |
| 5SYD Circularly permutated azurin (cpAz) based on P. aeruginosa azurin sequence Deposited 2016-08-10 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
63–148（86 aa）
Fragment：unp residues 63-148; 1-38
|
Not recorded | CU COPPER (II) ION × 3 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；2 uL of 5 mM protein was mixed with equal amount of well buffer (0.1 M Tris, 0.1 M LiNO 3, 0.01 M CUSO 4 with 35% polyethylene glycol 10000), and crystalized using the hanging drop method, with 300 uL well buffer in the crystallization tray.
|
Resolution 2.40 Å R-free 0.251 |
| 5SYD Circularly permutated azurin (cpAz) based on P. aeruginosa azurin sequence Deposited 2016-08-10 | Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics | Assembly 2 Insufficient information Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
63–148（86 aa）
Fragment：unp residues 63-148; 1-38
|
Not recorded | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；2 uL of 5 mM protein was mixed with equal amount of well buffer (0.1 M Tris, 0.1 M LiNO 3, 0.01 M CUSO 4 with 35% polyethylene glycol 10000), and crystalized using the hanging drop method, with 300 uL well buffer in the crystallization tray.
|
Resolution 2.40 Å R-free 0.251 |
| 5YT7 crystal structure of circularly permutated Azurin 3 Deposited 2017-11-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–134（114 aa）
|
Not recorded | CU COPPER (II) ION × 1 CA CALCIUM ION × 1 NO3 NITRATE ION × 1 CL CHLORIDE ION × 1 NA SODIUM ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；0.08 M NaOAc, 0.24 M LiNO3, 0.24 M CaCl2, 20% polyethylene glycol 8000
|
Resolution 1.66 Å R-free 0.217 |
| 5YT7 crystal structure of circularly permutated Azurin 3 Deposited 2017-11-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–134（114 aa）
|
Not recorded | CU COPPER (II) ION × 1 CA CALCIUM ION × 2 NO3 NITRATE ION × 2 CL CHLORIDE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；0.08 M NaOAc, 0.24 M LiNO3, 0.24 M CaCl2, 20% polyethylene glycol 8000
|
Resolution 1.66 Å R-free 0.217 |
| 5YT7 crystal structure of circularly permutated Azurin 3 Deposited 2017-11-17 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–134（114 aa）
|
Not recorded | CU COPPER (II) ION × 1 NO3 NITRATE ION × 1 NA SODIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；0.08 M NaOAc, 0.24 M LiNO3, 0.24 M CaCl2, 20% polyethylene glycol 8000
|
Resolution 1.66 Å R-free 0.217 |
| 5YT7 crystal structure of circularly permutated Azurin 3 Deposited 2017-11-17 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–134（114 aa）
|
Not recorded | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；293 K；0.08 M NaOAc, 0.24 M LiNO3, 0.24 M CaCl2, 20% polyethylene glycol 8000
|
Resolution 1.66 Å R-free 0.217 |
| 6GYI Azurin fom Pseudomonas aeruginosa treated with hydrosulfide Deposited 2018-06-29 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；30% PEG 4000, 100 mM NaHEPES pH 6,5, 200 mM CaCl2, 10 mM Na2S. Cryoprotectant: 10% PEG 4000, 20% PEG 400, 100 mM NaHEPES pH 6,5, 200 mM CaCl2
|
Resolution 1.60 Å R-free 0.243 |
| 6GYI Azurin fom Pseudomonas aeruginosa treated with hydrosulfide Deposited 2018-06-29 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；30% PEG 4000, 100 mM NaHEPES pH 6,5, 200 mM CaCl2, 10 mM Na2S. Cryoprotectant: 10% PEG 4000, 20% PEG 400, 100 mM NaHEPES pH 6,5, 200 mM CaCl2
|
Resolution 1.60 Å R-free 0.243 |
| 6GYI Azurin fom Pseudomonas aeruginosa treated with hydrosulfide Deposited 2018-06-29 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；30% PEG 4000, 100 mM NaHEPES pH 6,5, 200 mM CaCl2, 10 mM Na2S. Cryoprotectant: 10% PEG 4000, 20% PEG 400, 100 mM NaHEPES pH 6,5, 200 mM CaCl2
|
Resolution 1.60 Å R-free 0.243 |
| 6GYI Azurin fom Pseudomonas aeruginosa treated with hydrosulfide Deposited 2018-06-29 | Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 6.5；293 K；30% PEG 4000, 100 mM NaHEPES pH 6,5, 200 mM CaCl2, 10 mM Na2S. Cryoprotectant: 10% PEG 4000, 20% PEG 400, 100 mM NaHEPES pH 6,5, 200 mM CaCl2
|
Resolution 1.60 Å R-free 0.243 |
| 6IAV CO-AZURIN FROM PSEUDOMONAS AERUGINOSA TREATED WITH HYDROSULFIDE Deposited 2018-11-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–148（127 aa）
|
Not recorded | CO COBALT (II) ION × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；30% PEG 4000, 100 MM TRIS PH 7.5, 200 MM CACL2, 10 MM NA2S. CRYOPROTECTANT: 10% PEG 4000, 20% PEG 400, 100 MM TRIS PH 7.5, 200 MM CACL2, PH 7.5
|
Resolution 2.00 Å R-free 0.310 |
| 6IAV CO-AZURIN FROM PSEUDOMONAS AERUGINOSA TREATED WITH HYDROSULFIDE Deposited 2018-11-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–148（127 aa）
|
Not recorded | CO COBALT (II) ION × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；30% PEG 4000, 100 MM TRIS PH 7.5, 200 MM CACL2, 10 MM NA2S. CRYOPROTECTANT: 10% PEG 4000, 20% PEG 400, 100 MM TRIS PH 7.5, 200 MM CACL2, PH 7.5
|
Resolution 2.00 Å R-free 0.310 |
| 6IAV CO-AZURIN FROM PSEUDOMONAS AERUGINOSA TREATED WITH HYDROSULFIDE Deposited 2018-11-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
22–148（127 aa）
|
Not recorded | CO COBALT (II) ION × 1 CA CALCIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；30% PEG 4000, 100 MM TRIS PH 7.5, 200 MM CACL2, 10 MM NA2S. CRYOPROTECTANT: 10% PEG 4000, 20% PEG 400, 100 MM TRIS PH 7.5, 200 MM CACL2, PH 7.5
|
Resolution 2.00 Å R-free 0.310 |
| 6IAV CO-AZURIN FROM PSEUDOMONAS AERUGINOSA TREATED WITH HYDROSULFIDE Deposited 2018-11-27 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
22–148（127 aa）
|
Not recorded | CO COBALT (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.5；293 K；30% PEG 4000, 100 MM TRIS PH 7.5, 200 MM CACL2, 10 MM NA2S. CRYOPROTECTANT: 10% PEG 4000, 20% PEG 400, 100 MM TRIS PH 7.5, 200 MM CACL2, PH 7.5
|
Resolution 2.00 Å R-free 0.310 |
| 6MJR Azurin 122W/124F/126Re Deposited 2018-09-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–148（127 aa）
|
Mutation：W48F, Y72F, H83Q, Y108F, K122W, T124F, T126H | CU COPPER (II) ION × 1 REQ (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 4.5；294 K；0.1 M NaOAc, 120 mM/150 mM Li2SO4, and 48.6% PEG 400/27.7% PEG 8000 at pH 4.5
|
Resolution 2.01 Å R-free 0.189 |
| 6MJR Azurin 122W/124F/126Re Deposited 2018-09-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–148（127 aa）
|
Mutation：W48F, Y72F, H83Q, Y108F, K122W, T124F, T126H | CU COPPER (II) ION × 1 REQ (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 4.5；294 K；0.1 M NaOAc, 120 mM/150 mM Li2SO4, and 48.6% PEG 400/27.7% PEG 8000 at pH 4.5
|
Resolution 2.01 Å R-free 0.189 |
| 6MJR Azurin 122W/124F/126Re Deposited 2018-09-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
22–148（127 aa）
|
Mutation：W48F, Y72F, H83Q, Y108F, K122W, T124F, T126H | CU COPPER (II) ION × 1 REQ (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 4.5；294 K；0.1 M NaOAc, 120 mM/150 mM Li2SO4, and 48.6% PEG 400/27.7% PEG 8000 at pH 4.5
|
Resolution 2.01 Å R-free 0.189 |
| 6MJR Azurin 122W/124F/126Re Deposited 2018-09-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
22–148（127 aa）
|
Mutation：W48F, Y72F, H83Q, Y108F, K122W, T124F, T126H | CU COPPER (II) ION × 1 REQ (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 4.5；294 K；0.1 M NaOAc, 120 mM/150 mM Li2SO4, and 48.6% PEG 400/27.7% PEG 8000 at pH 4.5
|
Resolution 2.01 Å R-free 0.189 |
| 6MJS Azurin 122W/124W/126Re Deposited 2018-09-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–148（127 aa）
|
Mutation：W48F, Y72F, H83Q, Y108F, K122W, T124W, T126H | CU COPPER (II) ION × 1 REQ (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.2；294 K；100 mM imidiazole, 100 mM LiNO3, 6.25 mM CuCl2, 28% PEG 4000
|
Resolution 1.85 Å R-free 0.198 |
| 6MJS Azurin 122W/124W/126Re Deposited 2018-09-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–148（127 aa）
|
Mutation：W48F, Y72F, H83Q, Y108F, K122W, T124W, T126H | CU COPPER (II) ION × 1 REQ (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.2；294 K；100 mM imidiazole, 100 mM LiNO3, 6.25 mM CuCl2, 28% PEG 4000
|
Resolution 1.85 Å R-free 0.198 |
| 6MJS Azurin 122W/124W/126Re Deposited 2018-09-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
22–148（127 aa）
|
Mutation：W48F, Y72F, H83Q, Y108F, K122W, T124W, T126H | CU COPPER (II) ION × 1 REQ (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.2；294 K；100 mM imidiazole, 100 mM LiNO3, 6.25 mM CuCl2, 28% PEG 4000
|
Resolution 1.85 Å R-free 0.198 |
| 6MJS Azurin 122W/124W/126Re Deposited 2018-09-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
22–148（127 aa）
|
Mutation：W48F, Y72F, H83Q, Y108F, K122W, T124W, T126H | CU COPPER (II) ION × 1 REQ (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 7.2；294 K；100 mM imidiazole, 100 mM LiNO3, 6.25 mM CuCl2, 28% PEG 4000
|
Resolution 1.85 Å R-free 0.198 |
| 6MJT Azurin 122F/124W/126Re Deposited 2018-09-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
22–148（127 aa）
|
Mutation：W48F, Y72F, H83Q, Y108F, K122F, T124W, T126H | CU COPPER (II) ION × 1 REQ (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 4.5；294 K；0.1 M NaOAc, 120 mM/150 mM Li2SO4, and 48.6% PEG 400/27.7% PEG 8000 at pH 4.5
|
Resolution 1.89 Å R-free 0.245 |
| 6MJT Azurin 122F/124W/126Re Deposited 2018-09-21 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
22–148（127 aa）
|
Mutation：W48F, Y72F, H83Q, Y108F, K122F, T124W, T126H | CU COPPER (II) ION × 1 REQ (1,10 PHENANTHROLINE)-(TRI-CARBON MONOXIDE) RHENIUM (I) × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION；pH 4.5；294 K；0.1 M NaOAc, 120 mM/150 mM Li2SO4, and 48.6% PEG 400/27.7% PEG 8000 at pH 4.5
|
Resolution 1.89 Å R-free 0.245 |
| 7TC6 All Phe-Azurin variant - F15W Deposited 2021-12-22 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric |
Chain A
22–148（127 aa）
Chain B
22–148（127 aa）
|
Mutation：F15W, W48F, Y72F, H83Q, Y108F, T124H Mutation：F15W, W48F, Y72F, H83Q, Y108F, T124H | CU COPPER (II) ION × 3 NO3 NITRATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, SITTING DROP；293 K；20 mg/mL protein in 0.1 M NaOAc (pH 5.0), 26.5-29% PEG 4000, 100 mM lithium nitrate, 10 mM copper sulfate, and 100 mM tris (pH 8.0)
|
Resolution 1.85 Å R-free 0.243 |
| 7TNC M13F/G116F Pseudomonas aeruginosa azurin Deposited 2022-01-20 | Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
21–148（128 aa）
|
Mutation：M13F, G116F | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 4 CU COPPER (II) ION × 8 CL CHLORIDE ION × 4 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；The 1.4 mM apo-protein was prepared in 100 mM pH=5.6 NaOAc buffer. For each well, add 300 uL reservoir buffer containing 100 mM pH=8.0 Tris-HCl, 10 mM CuSO4 and 20% PEG-4000. 3 uL of protein stock was mixed with 1 uL reservoir buffer on the glass slides and sealed on the wells. Diamond-shaped blue crystals grew within 4 days.
|
Resolution 1.47 Å R-free 0.237 |
| 7U2F G116F Pseudomonas aeruginosa azurin Deposited 2022-02-23 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
21–148（128 aa）
Fragment：UNP residues 21-148
|
Mutation：G116F | CU COPPER (II) ION × 2 TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 8；277 K；3 uL 1.4 mM in 100 mM sodium acetate, pH 5.6 + 1 uL reservoir buffer (20% PEG4000, 100 mM Tris-HCl, pH 8.0, 10 mM copper(II) sulfate, 100 mM lithium nitrate) against 250 uL reservoir buffer
|
Resolution 2.20 Å R-free 0.256 |
| 7YGI Crystal structure of p53 DBD domain in complex with azurin Deposited 2022-07-11 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric |
Chain C
23–145（123 aa）
Chain D
23–145（123 aa）
|
Not recorded | NA SODIUM ION × 2 ZN ZINC ION × 2 K POTASSIUM ION × 2 PO4 PHOSPHATE ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
BATCH MODE；298 K；0.05M Na2HPO4+19.5% PEG3350
|
Resolution 2.10 Å R-free 0.270 |
| 8F5K Azurin from Pseudomonas aeruginosa, Y72F/Y108F/F110A mutant Deposited 2022-11-14 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–148（148 aa）
|
Mutation：Y72F,Y108F,F110A | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；22-25 % (w/v) PEG 3350, 0.1 M Hepes, pH 7.0, 0.2 M sodium formate
|
Resolution 1.25 Å R-free 0.179 |
| 8F5K Azurin from Pseudomonas aeruginosa, Y72F/Y108F/F110A mutant Deposited 2022-11-14 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–148（148 aa）
|
Mutation：Y72F,Y108F,F110A | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；22-25 % (w/v) PEG 3350, 0.1 M Hepes, pH 7.0, 0.2 M sodium formate
|
Resolution 1.25 Å R-free 0.179 |
| 8F5K Azurin from Pseudomonas aeruginosa, Y72F/Y108F/F110A mutant Deposited 2022-11-14 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain C
1–148（148 aa）
|
Mutation：Y72F,Y108F,F110A | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；22-25 % (w/v) PEG 3350, 0.1 M Hepes, pH 7.0, 0.2 M sodium formate
|
Resolution 1.25 Å R-free 0.179 |
| 8F5K Azurin from Pseudomonas aeruginosa, Y72F/Y108F/F110A mutant Deposited 2022-11-14 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain D
1–148（148 aa）
|
Mutation：Y72F,Y108F,F110A | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；22-25 % (w/v) PEG 3350, 0.1 M Hepes, pH 7.0, 0.2 M sodium formate
|
Resolution 1.25 Å R-free 0.179 |
| 8F5L Azurin from Pseudomonas aeruginosa, Y72F/Y108F/F110L mutant Deposited 2022-11-14 | Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
1–148（148 aa）
|
Mutation：Y72F,Y108F,F110L | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 1 CU COPPER (II) ION × 2 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；22-25 % (w/v) PEG 3350, 0.1 M Hepes, pH 7.0, 0.2 M sodium formate
|
Resolution 1.15 Å R-free 0.174 |
| 8F5L Azurin from Pseudomonas aeruginosa, Y72F/Y108F/F110L mutant Deposited 2022-11-14 | Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics | Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain B
1–148（148 aa）
|
Mutation：Y72F,Y108F,F110L | CU COPPER (II) ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7；293 K；22-25 % (w/v) PEG 3350, 0.1 M Hepes, pH 7.0, 0.2 M sodium formate
|
Resolution 1.15 Å R-free 0.174 |
| 9L6D the crystal structure of Azurin-LBT Deposited 2024-12-24 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 3 PDB declaration: trimeric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
Chain C
21–148（128 aa）
|
Not recorded | CU COPPER (II) ION × 3 TB TERBIUM(III) ION × 7 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；277 K；0.1M NaOAc pH5.6, 10% 2-propanol with 16% PEG6000
|
Resolution 2.38 Å R-free 0.245 |
| 9OH6 H117A/M121H Azurin with Cu(II), pH 7.7 Deposited 2025-05-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain B
21–148（128 aa）
Chain E
21–148（128 aa）
Chain H
21–148（128 aa）
Chain K
21–148（128 aa）
|
Mutation：H117A, M121H Mutation：H117A, M121H Mutation：H117A, M121H Mutation：H117A, M121H | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 4 CU COPPER (II) ION × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.7；277 K；Well buffer: 27% PEG 4000, 100 mM LiNO3, 50 mM Tris at pH 7.7
Protein stock: 1.5 mM in 25 mM Tris pH 7.8, 1.8 mM CuSO4
Crystallization drop: 1 uL protein stock + 3 uL well buffer
|
Resolution 2.04 Å R-free 0.233 |
| 9OH7 M13F/H117A/M121H Azurin with Cu(II), pH 7.4 Deposited 2025-05-02 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics | Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric |
Chain A
21–148（128 aa）
Chain B
21–148（128 aa）
Chain E
21–148（128 aa）
Chain G
21–148（128 aa）
|
Mutation：M13F, H117A, M121H Mutation：M13F, H117A, M121H Mutation：M13F, H117A, M121H Mutation：M13F, H117A, M121H | TRS 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL × 4 CU COPPER (II) ION × 8 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 7.4；277.15 K；Well buffer: 30% PEG 4000, 100 mM LiNO3, 50 mM Tris at pH 7.4
Protein stock: 1.5 mM in 25 mM Tris pH 7.8, 1.8 mM CuSO4
Crystallization drop: 1.5 uL protein stock + 1.5 uL well buffer
|
Resolution 2.00 Å R-free 0.265 |
110 other PDB entries and 259 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | AZUR_PSEAE |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 1–128； UniProt 21–148 Author chain B； PDBConstruct 1–128； UniProt 21–148 Author chain C； PDBConstruct 1–128； UniProt 21–148 Author chain D； PDBConstruct 1–128； UniProt 21–148 Author chain E； PDBConstruct 1–128； UniProt 21–148 Author chain F； PDBConstruct 1–128； UniProt 21–148 Author chain G； PDBConstruct 1–128； UniProt 21–148 Author chain H； PDBConstruct 1–128； UniProt 21–148 Author chain I； PDBConstruct 1–128； UniProt 21–148 Author chain K； PDBConstruct 1–128； UniProt 21–148 Author chain L； PDBConstruct 1–128； UniProt 21–148 Author chain M； PDBConstruct 1–128； UniProt 21–148 |
