1jqs
;Fitting of L11 protein and elongation factor G (domain G' and V) in the cryo-em map of E. coli 70S ribosome bound with EF-G and GMPPCP, a nonhydrolysable GTP analog ;
SAXS 散射曲线 SAXS Profile
P(r) 距离分布 P(r) Distribution
1. 结构基本信息 1. Structure Basics
| 条目编号 entry_id | 1jqs |
| 沉积日期 deposition_date | 2001-08-07 |
| 结构标题 title | ;Fitting of L11 protein and elongation factor G (domain G' and V) in the cryo-em map of E. coli 70S ribosome bound with EF-G and GMPPCP, a nonhydrolysable GTP analog ; |
| 关键词 keywords | L11, EF-G, cryo-EM, 70S E.coli ribosome, GTP state, RIBOSOME; RIBOSOME |
| 实验方法 method | ELECTRON MICROSCOPY |
2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)
| 回转半径 Rg (Guinier) rg_guinier | 27.94 Å |
| 回转半径 Rg (电子) rg_electron | 27.79 Å |
| 零角强度 I(0) i0 | 10000500.00 |
| 分子量 molecular_weight | 25500.0 kDa |
| 排除体积 excluded_volume | 31514 ų |
| 包络体积 envelope_volume | 27351 ų |
| 水化壳体积 shell_volume | 10463 ų |
| 包络直径 envelope_diameter | 91.0 Å |
| 壳层 Rg shell_rg | 27.84 Å |
| 包络 Rg envelope_rg | 26.12 Å |
| 形状 Rg shape_rg | 27.66 Å |
| 总 Rg total_rg | 27.80 Å |
| 总原子数 total_atoms | — |
| 残基数 n_residues | — |
| 球谐函数阶数 n_harmonics | 20 |
| q 范围 q_range | — – 0.5000 Å−1 |
| 数据点数 n_points | 101 |
| 壳层类型 shell_type | directional |
| 溶剂电子密度 solvent_density | 0.3340 e/ų |
| 壳层衬度 contrast_shell | 0.0300 e/ų |
| CRYSOL 版本 crysol_version | 4.1.3 |
3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)
| 最大尺寸 Dmax dmax | 89.3 Å |
| Rg (实空间) rg_real | 28.19 Å |
| Rg 误差 (实空间) rg_real_error | 0.68 Å |
| I(0) (实空间) i0_real | 1.0000e+07 |
| I(0) 误差 (实空间) i0_real_error | 1.3830e+05 |
| Rg (倒空间) rg_reciprocal | 28.12 Å |
| I(0) (倒空间) i0_reciprocal | 10000000.0000 |
| 解质量估计 total_estimate | 0.8037 |
| 解质量评级 solution_quality | GOOD a GOOD solution |
| P(r) 峰数 n_peaks | 3 |
| 主峰位置 r_peak_primary | 39.2 Å |
| 偏度 Skewness skewness | 0.326 |
| 峰度 Kurtosis kurtosis | -0.486 |
| 角度范围 angular_range | — – 0.2850 Å−1 |
| 当前正则化参数 α current_alpha | 0.0000 |
| 最高正则化参数 α highest_alpha | 400300.0000 |
| 实空间数据点数 n_real_points | 58 |
| GNOM 版本 gnom_version | 4.1.3 |
| 质量判据 quality_criteria | AN1: 0.000; Oscil: 0.650; Stabil: 0.998; Sysdev: 1.000; Positv: 1.000; Valcen: 0.646; Smooth: 0.854 |
4. 晶体学与实验 4. Crystallography & Experiment
5. 实体与聚合体信息 (3)
6. 折叠分类 (SCOP + CATH) 3 domains
SCOP 2.08 (3 domains)
| 结构域编号 domain_id | d1jqsa_ |
| 类 Class class | i — Low resolution protein structures |
| 折叠类型 Fold fold | i.1 — Ribosome and ribosomal fragments |
| 超家族 Superfamily superfamily | i.1.1 — Ribosome and ribosomal fragments |
| 家族 Family family | i.1.1.1 — Ribosome complexes |
| 结构域编号 domain_id | d1jqsb_ |
| 类 Class class | i — Low resolution protein structures |
| 折叠类型 Fold fold | i.1 — Ribosome and ribosomal fragments |
| 超家族 Superfamily superfamily | i.1.1 — Ribosome and ribosomal fragments |
| 家族 Family family | i.1.1.1 — Ribosome complexes |
| 结构域编号 domain_id | d1jqsc_ |
| 类 Class class | i — Low resolution protein structures |
| 折叠类型 Fold fold | i.1 — Ribosome and ribosomal fragments |
| 超家族 Superfamily superfamily | i.1.1 — Ribosome and ribosomal fragments |
| 家族 Family family | i.1.1.1 — Ribosome complexes |