1mih

A ROLE FOR CHEY GLU 89 IN CHEZ-MEDIATED DEPHOSPHORYLATION OF THE E. COLI CHEMOTAXIS RESPONSE REGULATOR CHEY

Method: X-RAY DIFFRACTION D_max: 95.0 Å Quality: REASONABLE

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Chemotaxis protein cheY

Escherichia coli

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count	Chain A； UniProt 0–128	Mutation：N59R	MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 8.4；293 K；1.8 M Ammonium sulfate, 1 mM MnCl2, 10 mM NaF, 1 mM BeCl2, 5% glycerol, 10 mg/ml CheY N59R, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.70 Å R-free 0.277
2	Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count	Chain B； UniProt 0–128	Mutation：N59R	MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 2 BEF BERYLLIUM TRIFLUORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 8.4；293 K；1.8 M Ammonium sulfate, 1 mM MnCl2, 10 mM NaF, 1 mM BeCl2, 5% glycerol, 10 mg/ml CheY N59R, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.70 Å R-free 0.277

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 1MIH	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
1A0O CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY Deposited 1997-12-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 1–128（128 aa）	Not recorded	MN MANGANESE (II) ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 5.5；PROTEIN WAS CRYSTALLIZED FROM 20% PEG MME 5K, 0.1 M MALONIC ACID, 0.1 M MES BUFFER PH 5.5, 0.02 M DTT, 0.01 M MANGANESE CHLORIDE	Resolution 2.95 Å R-free 0.235
1A0O CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY Deposited 1997-12-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain C 1–128（128 aa）	Not recorded	MN MANGANESE (II) ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 5.5；PROTEIN WAS CRYSTALLIZED FROM 20% PEG MME 5K, 0.1 M MALONIC ACID, 0.1 M MES BUFFER PH 5.5, 0.02 M DTT, 0.01 M MANGANESE CHLORIDE	Resolution 2.95 Å R-free 0.235
1A0O CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY Deposited 1997-12-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain E 1–128（128 aa）	Not recorded	MN MANGANESE (II) ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 5.5；PROTEIN WAS CRYSTALLIZED FROM 20% PEG MME 5K, 0.1 M MALONIC ACID, 0.1 M MES BUFFER PH 5.5, 0.02 M DTT, 0.01 M MANGANESE CHLORIDE	Resolution 2.95 Å R-free 0.235
1A0O CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY Deposited 1997-12-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain G 1–128（128 aa）	Not recorded	MN MANGANESE (II) ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 5.5；PROTEIN WAS CRYSTALLIZED FROM 20% PEG MME 5K, 0.1 M MALONIC ACID, 0.1 M MES BUFFER PH 5.5, 0.02 M DTT, 0.01 M MANGANESE CHLORIDE	Resolution 2.95 Å R-free 0.235
1AB5 STRUCTURE OF CHEY MUTANT F14N, V21T Deposited 1997-02-04	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 4–128（125 aa）	Mutation：F14N, V21T	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.2；PROTEIN WAS CRYSTALLIZED FROM AMMONIUM SULFATE, 2.6-3.0M IN TRIS-HCL BUFFER AT PH 7.2.	Resolution 2.40 Å R-free 0.264
1AB5 STRUCTURE OF CHEY MUTANT F14N, V21T Deposited 1997-02-04	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 4–128（125 aa）	Mutation：F14N, V21T	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.2；PROTEIN WAS CRYSTALLIZED FROM AMMONIUM SULFATE, 2.6-3.0M IN TRIS-HCL BUFFER AT PH 7.2.	Resolution 2.40 Å R-free 0.264
1AB6 STRUCTURE OF CHEY MUTANT F14N, V86T Deposited 1997-02-04	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 4–128（125 aa）	Mutation：F14N, V86T	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.2；293 K；THE PROTEIN WAS CRYSTALLIZED WITH 3.0M AMMONIUM SULFATE, PH7.2 AT 20C., temperature 293K	Resolution 2.20 Å R-free 0.280
1AB6 STRUCTURE OF CHEY MUTANT F14N, V86T Deposited 1997-02-04	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 4–128（125 aa）	Mutation：F14N, V86T	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.2；293 K；THE PROTEIN WAS CRYSTALLIZED WITH 3.0M AMMONIUM SULFATE, PH7.2 AT 20C., temperature 293K	Resolution 2.20 Å R-free 0.280
1BDJ COMPLEX STRUCTURE OF HPT DOMAIN AND CHEY Deposited 1998-05-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 1–128（128 aa）	Not recorded	SO4 SULFATE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 7；pH 7.0	Resolution 2.68 Å R-free 0.250
1C4W 1.9 A STRUCTURE OF A-THIOPHOSPHONATE MODIFIED CHEY D57C Deposited 1999-09-28	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 2–129（128 aa）	Mutation：D57(CYQ) Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；298 K；PEG 6000, SODIUM ACETATE, PIPES, pH 6.9, VAPOR DIFFUSION/HANGING DROP, temperature 298K	Resolution 1.84 Å
1CEY ASSIGNMENTS, SECONDARY STRUCTURE, GLOBAL FOLD, AND DYNAMICS OF CHEMOTAXIS Y PROTEIN USING THREE-AND FOUR-DIMENSIONAL HETERONUCLEAR (13C,15N) NMR SPECTROSCOPY Deposited 1994-11-23	Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–128（128 aa）	Not recorded	No recorded non-water small molecule	SOLUTION NMR mmCIF provides none of the parsed conditions	Resolution not provided
1CHN MAGNESIUM BINDING TO THE BACTERIAL CHEMOTAXIS PROTEIN CHEY RESULTS IN LARGE CONFORMATIONAL CHANGES INVOLVING ITS FUNCTIONAL SURFACE Deposited 1994-04-20	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–128（128 aa）	Not recorded	MG MAGNESIUM ION × 1	X-RAY DIFFRACTION mmCIF provides none of the parsed conditions	Resolution 1.76 Å
1CYE THREE DIMENSIONAL STRUCTURE OF CHEMOTACTIC CHE Y PROTEIN IN AQUEOUS SOLUTION BY NUCLEAR MAGNETIC RESONANCE METHODS Deposited 1994-10-21	Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 2–128（127 aa）	Not recorded	No recorded non-water small molecule	SOLUTION NMR mmCIF provides none of the parsed conditions	Resolution not provided
1D4Z CRYSTAL STRUCTURE OF CHEY-95IV, A HYPERACTIVE CHEY MUTANT Deposited 1999-10-06	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–128（128 aa）	Mutation：I95V	SO4 SULFATE ION × 3	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；278 K；AMMONIUM SULPHATE, PEG4000, SODIUM ACETATE, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 278K	Resolution 1.90 Å R-free 0.227
1DJM SOLUTION STRUCTURE OF BEF3-ACTIVATED CHEY FROM ESCHERICHIA COLI Deposited 1999-12-03	Different construct Different mutation/modification Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–129（129 aa）	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 6.7；298 K；Ionic strength (raw mmCIF value) 16 mM BECL2, 100 mM NAF, 20 mM MGCL2；Pressure AMBIENT NMR measurement conditions pH 6.7；298 K；Ionic strength (raw mmCIF value) 16 mM BECL2, 100 mM NAF, 20 mM MGCL2；Pressure AMBIENT NMR measurement conditions pH 6.7；298 K；Ionic strength (raw mmCIF value) 16 mM BECL2, 100 mM NAF, 20 mM MGCL2；Pressure AMBIENT NMR sample composition 4 MM CHEY U-15N, 13C, 16 MM BECL2, 100 MM NAF, 20 MM MGCL2, PH 6.7 NMR sample composition 3 MM U-15N, 16 MM BECL2, 100 MM NAF, 20 MM MGCL2, PH 6.7 NMR sample composition 3 MM U-10% 13C, 16 MM BECL2, 100 MM NAF, 20 MM MGCL2, PH 6.7 NMR sample composition 2 MM CHEY, 16 MM BECL2, 100 MM NAF, 20 MM MGCL2, PH 6.7	Resolution not provided
1EAY CHEY-BINDING (P2) DOMAIN OF CHEA IN COMPLEX WITH CHEY FROM ESCHERICHIA COLI Deposited 1998-04-23	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 1–128（128 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7；PROTEIN WAS CRYSTALLIZED FROM .63 M NAH2PO4/1.17 M K2HPO4, 10 MM NH4CL, PH 7.0	Resolution 2.00 Å
1EAY CHEY-BINDING (P2) DOMAIN OF CHEA IN COMPLEX WITH CHEY FROM ESCHERICHIA COLI Deposited 1998-04-23	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 1–128（128 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7；PROTEIN WAS CRYSTALLIZED FROM .63 M NAH2PO4/1.17 M K2HPO4, 10 MM NH4CL, PH 7.0	Resolution 2.00 Å
1EHC STRUCTURE OF SIGNAL TRANSDUCTION PROTEIN CHEY Deposited 1996-03-05	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–128（128 aa）	Mutation：D13K	SO4 SULFATE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 8.3；pH 8.3	Resolution 2.26 Å
1F4V CRYSTAL STRUCTURE OF ACTIVATED CHEY BOUND TO THE N-TERMINUS OF FLIM Deposited 2000-06-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 1–128（128 aa）	Not recorded	MG MAGNESIUM ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；ammonium sulfate, glycerol, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.22 Å R-free 0.258
1F4V CRYSTAL STRUCTURE OF ACTIVATED CHEY BOUND TO THE N-TERMINUS OF FLIM Deposited 2000-06-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 1–128（128 aa）	Not recorded	MG MAGNESIUM ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 GOL GLYCEROL × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；ammonium sulfate, glycerol, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.22 Å R-free 0.258
1F4V CRYSTAL STRUCTURE OF ACTIVATED CHEY BOUND TO THE N-TERMINUS OF FLIM Deposited 2000-06-10	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain C 1–128（128 aa）	Not recorded	MG MAGNESIUM ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；ammonium sulfate, glycerol, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.22 Å R-free 0.258
1FFG CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY AT 2.1 A RESOLUTION Deposited 2000-07-25	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 1–128（128 aa）	Not recorded	MN MANGANESE (II) ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；297 K；20% PEG MME 5K, 0.1 M MALONIC ACID, 0.1 M MES BUFFER 0.02 M DTT, 0.01 M MANGANESE CHLORIDE, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K	Resolution 2.10 Å R-free 0.220
1FFG CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY AT 2.1 A RESOLUTION Deposited 2000-07-25	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain C 1–128（128 aa）	Not recorded	MN MANGANESE (II) ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；297 K；20% PEG MME 5K, 0.1 M MALONIC ACID, 0.1 M MES BUFFER 0.02 M DTT, 0.01 M MANGANESE CHLORIDE, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K	Resolution 2.10 Å R-free 0.220
1FFS CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY FROM CRYSTALS SOAKED IN ACETYL PHOSPHATE Deposited 2000-07-26	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 1–128（128 aa） Fragment：RESIDUES 124-257	Not recorded	MN MANGANESE (II) ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；297 K；20% PEG MME 5K, 0.1 M MALONIC ACID, 0.1 M MES BUFFER 0.02 M DTT, 0.01 M MANGANESE CHLORIDE , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K	Resolution 2.40 Å R-free 0.255
1FFS CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY FROM CRYSTALS SOAKED IN ACETYL PHOSPHATE Deposited 2000-07-26	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain C 1–128（128 aa） Fragment：RESIDUES 124-257	Not recorded	MN MANGANESE (II) ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；297 K；20% PEG MME 5K, 0.1 M MALONIC ACID, 0.1 M MES BUFFER 0.02 M DTT, 0.01 M MANGANESE CHLORIDE , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K	Resolution 2.40 Å R-free 0.255
1FQW CRYSTAL STRUCTURE OF ACTIVATED CHEY Deposited 2000-09-07	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–128（128 aa）	Not recorded	MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；Ammonium sulfate, Tris buffer, MnCl2, glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.37 Å R-free 0.240
1FQW CRYSTAL STRUCTURE OF ACTIVATED CHEY Deposited 2000-09-07	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 1–128（128 aa）	Not recorded	MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；Ammonium sulfate, Tris buffer, MnCl2, glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.37 Å R-free 0.240
1HEY INVESTIGATING THE STRUCTURAL DETERMINANTS OF THE P21-LIKE TRIPHOSPHATE AND MG2+ BINDING SITE Deposited 1995-04-07	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–128（128 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION mmCIF provides none of the parsed conditions	Resolution 2.24 Å
1KMI CRYSTAL STRUCTURE OF AN E.COLI CHEMOTAXIS PROTEIN, CHEZ Deposited 2001-12-16	Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain Y 0–128（129 aa）	Not recorded	MG MAGNESIUM ION × 2 BEF BERYLLIUM TRIFLUORIDE ION × 2 BCN BICINE × 2	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；277 K；isopropanol, ammonium acetate, bicine, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.90 Å R-free 0.298
1U8T Crystal structure of CheY D13K Y106W alone and in complex with a FliM peptide Deposited 2004-08-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 1–128（128 aa）	Mutation：D13K Y106W Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.9；277 K；PEG 3350, ammonium sulfate, Tris, VAPOR DIFFUSION, temperature 277K, pH 7.90	Resolution 1.50 Å R-free 0.273
1U8T Crystal structure of CheY D13K Y106W alone and in complex with a FliM peptide Deposited 2004-08-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 1–128（128 aa）	Mutation：D13K Y106W Non-standard monomer：Yes (specific site not provided by mmCIF)	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.9；277 K；PEG 3350, ammonium sulfate, Tris, VAPOR DIFFUSION, temperature 277K, pH 7.90	Resolution 1.50 Å R-free 0.273
1U8T Crystal structure of CheY D13K Y106W alone and in complex with a FliM peptide Deposited 2004-08-06	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain C 1–128（128 aa）	Mutation：D13K Y106W Non-standard monomer：Yes (specific site not provided by mmCIF)	SO4 SULFATE ION × 3	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.9；277 K；PEG 3350, ammonium sulfate, Tris, VAPOR DIFFUSION, temperature 277K, pH 7.90	Resolution 1.50 Å R-free 0.273
1U8T Crystal structure of CheY D13K Y106W alone and in complex with a FliM peptide Deposited 2004-08-06	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain D 1–128（128 aa）	Mutation：D13K Y106W Non-standard monomer：Yes (specific site not provided by mmCIF)	SO4 SULFATE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.9；277 K；PEG 3350, ammonium sulfate, Tris, VAPOR DIFFUSION, temperature 277K, pH 7.90	Resolution 1.50 Å R-free 0.273
1U8T Crystal structure of CheY D13K Y106W alone and in complex with a FliM peptide Deposited 2004-08-06	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain C 1–128（128 aa） Chain D 1–128（128 aa）	Mutation：D13K Y106W Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：D13K Y106W Non-standard monomer：Yes (specific site not provided by mmCIF)	SO4 SULFATE ION × 4	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.9；277 K；PEG 3350, ammonium sulfate, Tris, VAPOR DIFFUSION, temperature 277K, pH 7.90	Resolution 1.50 Å R-free 0.273
1UDR CHEY MUTANT WITH LYS 91 REPLACED BY ASP, LYS 92 REPLACED BY ALA, ILE 96 REPLACED BY LYS AND ALA 98 REPLACED BY LEU (STABILIZING MUTATIONS IN HELIX 4) Deposited 1996-11-05	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric	Chain A 2–128（127 aa） Chain B 2–128（127 aa） Chain C 2–128（127 aa） Chain D 2–128（127 aa）	Mutation：K91D, K92A, I96K, A98L Mutation：K91D, K92A, I96K, A98L Mutation：K91D, K92A, I96K, A98L Mutation：K91D, K92A, I96K, A98L	No recorded non-water small molecule	X-RAY DIFFRACTION mmCIF provides none of the parsed conditions	Resolution 1.90 Å
1VLZ UNCOUPLED PHOSPHORYLATION AND ACTIVATION IN BACTERIAL CHEMOTAXIS: THE 2.1 ANGSTROM STRUCTURE OF A THREONINE TO ISOLEUCINE MUTANT AT POSITION 87 OF CHEY Deposited 1995-04-21	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 1–128（128 aa） Chain B 1–128（128 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION mmCIF provides none of the parsed conditions	Resolution 2.05 Å
1YMU SIGNAL TRANSDUCTION PROTEIN CHEY MUTANT WITH MET 17 REPLACED BY GLY (M17G) Deposited 1995-12-14	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain A 2–128（127 aa） Chain B 2–128（127 aa）	Mutation：INS(M0), M1R, A2S, M17G Mutation：INS(M0), M1R, A2S, M17G	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 7.2；pH 7.2	Resolution 2.30 Å
1YMV SIGNAL TRANSDUCTION PROTEIN CHEY MUTANT WITH PHE 14 REPLACED BY GLY, SER 15 REPLACED BY GLY, AND MET 17 REPLACED BY GLY Deposited 1995-12-14	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 2–128（127 aa）	Mutation：INS(M0), M1R, A2S, F14G, S15G, M17G	MG MAGNESIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 6.8；pH 6.8	Resolution 1.90 Å
1ZDM Crystal Structure of Activated CheY Bound to Xe Deposited 2005-04-14	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 0–128（129 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	MN MANGANESE (II) ION × 1 XE XENON × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；1.8 M ammonium sulfate, 5-10% glycerol, 100 mM Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.40 Å R-free 0.267
1ZDM Crystal Structure of Activated CheY Bound to Xe Deposited 2005-04-14	Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 0–128（129 aa）	Non-standard monomer：Yes (specific site not provided by mmCIF)	MN MANGANESE (II) ION × 1 XE XENON × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；1.8 M ammonium sulfate, 5-10% glycerol, 100 mM Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K	Resolution 2.40 Å R-free 0.267
3CHY CRYSTAL STRUCTURE OF ESCHERICHIA COLI CHEY REFINED AT 1.7-ANGSTROM RESOLUTION Deposited 1991-04-22	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–128（128 aa）	Not recorded	SO4 SULFATE ION × 3	X-RAY DIFFRACTION mmCIF provides none of the parsed conditions	Resolution 1.66 Å
5CHY STRUCTURE OF CHEMOTAXIS PROTEIN CHEY Deposited 1996-08-29	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–128（128 aa）	Mutation：Y106W	CA CALCIUM ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions pH 6；28% PEG 8000, 0.12 M CALCIUM ACETATE, 0.1 M CACODYLATE BUFFER, PH 6.0	Resolution 2.00 Å
6CHY STRUCTURE OF CHEMOTAXIS PROTEIN CHEY Deposited 1996-08-29	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 1–128（128 aa）	Mutation：T87I, Y106W	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions 18% PEG 3350 AND 0.2 M AMMONIUM SULFATE	Resolution 2.33 Å
6CHY STRUCTURE OF CHEMOTAXIS PROTEIN CHEY Deposited 1996-08-29	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain B 1–128（128 aa）	Mutation：T87I, Y106W	SO4 SULFATE ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions 18% PEG 3350 AND 0.2 M AMMONIUM SULFATE	Resolution 2.33 Å
6CHY STRUCTURE OF CHEMOTAXIS PROTEIN CHEY Deposited 1996-08-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric	Chain B 1–128（128 aa）	Mutation：T87I, Y106W	SO4 SULFATE ION × 2	X-RAY DIFFRACTION X-ray crystallization conditions 18% PEG 3350 AND 0.2 M AMMONIUM SULFATE	Resolution 2.33 Å

27 other PDB entries and 45 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	CHEY_ECOLI
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–129； UniProt 0–128 Author chain B； PDBConstruct 1–129； UniProt 0–128

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

Download Download SAXS (.dat) P(r) (.pr.out) CIF

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1mih
Deposition date deposition_date	2002-08-23
Structure title title	A ROLE FOR CHEY GLU 89 IN CHEZ-MEDIATED DEPHOSPHORYLATION OF THE E. COLI CHEMOTAXIS RESPONSE REGULATOR CHEY
Keywords keywords	Bacterial Chemotaxis, Response Regulator, Dephosphorylation, SIGNALING PROTEIN; SIGNALING PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	30.99 Å
Radius of gyration Rg (electron density) rg_electron	30.68 Å
Forward intensity I(0) i0	13140100.00
Molecular weight molecular_weight	28541.0 kDa
Excluded volume excluded_volume	35793 Å³
Envelope volume envelope_volume	48876 Å³
Hydration-shell volume shell_volume	13396 Å³
Envelope diameter envelope_diameter	92.3 Å
Shell Rg shell_rg	37.54 Å
Envelope Rg envelope_rg	29.56 Å
Shape Rg shape_rg	30.69 Å
Total Rg total_rg	31.31 Å
Total atoms total_atoms	1989
Residues n_residues	256
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	95.0 Å
Rg (real space) rg_real	31.34 Å
Rg uncertainty (real space) rg_real_error	0.90 Å
I(0) (real space) i0_real	1.3140e+07
I(0) uncertainty (real space) i0_real_error	2.0410e+05
Rg (reciprocal space) rg_reciprocal	31.20 Å
I(0) (reciprocal space) i0_reciprocal	13140000.0000
Solution quality estimate total_estimate	0.6191
Solution quality rating solution_quality	REASONABLE a REASONABLE solution
P(r) peaks n_peaks	3
Primary peak position r_peak_primary	18.6 Å
Skewness Skewness skewness	0.224
Kurtosis Kurtosis kurtosis	-1.322
Angular range angular_range	— – 0.2550 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	4182000.0000
Real-space data points n_real_points	52
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.024; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 0.089; Smooth: 0.886

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (5)

Entity 1 Entity 1 polymer

Description description	Chemotaxis protein cheY
Molecular weight formula_weight	14155.4 kDa
Mutation mutation	N59R
Source method src_method	man
Copy count entity_count	2

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A,B
Atom count atom_count	2033

MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWRMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAGASGYVVKPFTAATLEEKLNKIFEKLGM

来源 Source

来源生物 organism	Escherichia coli
NCBI Class编号 ncbi_taxonomy_id	562
宿主生物 host_organism	Escherichia coli

Entity 2 Entity 2 non-polymer

Description description	MANGANESE (II) ION
Molecular weight formula_weight	54.9 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 3 Entity 3 non-polymer

Description description	SULFATE ION
Molecular weight formula_weight	96.1 kDa
Source method src_method	syn
Copy count entity_count	3

Entity 4 Entity 4 non-polymer

Description description	BERYLLIUM TRIFLUORIDE ION
Molecular weight formula_weight	66.0 kDa
Source method src_method	syn
Copy count entity_count	2

Entity 5 Entity 5 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	44

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7. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

Domain ID domain_id	d1miha_
Class class	c — Alpha and beta proteins (a/b)
Fold Fold fold	c.23 — Flavodoxin-like
Superfamily Superfamily superfamily	c.23.1 — CheY-like
Family Family family	c.23.1.1 — CheY-related

Domain ID domain_id	d1mihb_
Class class	c — Alpha and beta proteins (a/b)
Fold Fold fold	c.23 — Flavodoxin-like
Superfamily Superfamily superfamily	c.23.1 — CheY-like
Family Family family	c.23.1.1 — CheY-related

CATH v4.4 (2 domains)

Domain ID domain_id	1mihA00
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	50 — Rossmann fold
Homologous superfamily homologous superfamily	2300 — Response regulator

Domain ID domain_id	1mihB00
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	50 — Rossmann fold
Homologous superfamily homologous superfamily	2300 — Response regulator

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8. Citations (1)

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Unit-cell length a length_a	53.504 Å
Unit-cell length b length_b	53.615 Å
Unit-cell length c length_c	160.890 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	8

Space group space_group	P 21 21 21
Space-group number int_tables_number	19
Crystal system crystal_system	—

Resolution resolution	2.700 Å
Low-resolution limit resolution_low	27.000 Å
R factor r_factor	0.2430
R_work r_work	0.2260
R_free r_free	0.2770

fit	/app/data/saxs_computation/mi/1mih/1mih.fit
pddf	/app/data/pr_computation/mi/1mih/1mih.pr.out
profile	/app/data/saxs_computation/mi/1mih/1mih.dat