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1vlz

UNCOUPLED PHOSPHORYLATION AND ACTIVATION IN BACTERIAL CHEMOTAXIS: THE 2.1 ANGSTROM STRUCTURE OF A THREONINE TO ISOLEUCINE MUTANT AT POSITION 87 OF CHEY

Method: X-RAY DIFFRACTION Dmax: 68.2 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

CHEY

Escherichia coli

UniProt P06143

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein homooligomer Homooligomer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count Chain A； UniProt 1–128 Chain B； UniProt 1–128 Not recorded No other associated polymer X-RAY DIFFRACTION mmCIF provides none of the parsed experimental conditions Resolution 2.05 Å

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 1VLZ Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
1A0O CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY Deposited 1997-12-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–128（128 aa）
Not recorded MN MANGANESE (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.5；PROTEIN WAS CRYSTALLIZED FROM 20% PEG MME 5K, 0.1 M MALONIC ACID, 0.1 M MES BUFFER PH 5.5, 0.02 M DTT, 0.01 M MANGANESE CHLORIDE
Resolution 2.95 Å R-free 0.235
1A0O CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY Deposited 1997-12-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1–128（128 aa）
Not recorded MN MANGANESE (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.5；PROTEIN WAS CRYSTALLIZED FROM 20% PEG MME 5K, 0.1 M MALONIC ACID, 0.1 M MES BUFFER PH 5.5, 0.02 M DTT, 0.01 M MANGANESE CHLORIDE
Resolution 2.95 Å R-free 0.235
1A0O CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY Deposited 1997-12-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain E 1–128（128 aa）
Not recorded MN MANGANESE (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.5；PROTEIN WAS CRYSTALLIZED FROM 20% PEG MME 5K, 0.1 M MALONIC ACID, 0.1 M MES BUFFER PH 5.5, 0.02 M DTT, 0.01 M MANGANESE CHLORIDE
Resolution 2.95 Å R-free 0.235
1A0O CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY Deposited 1997-12-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain G 1–128（128 aa）
Not recorded MN MANGANESE (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 5.5；PROTEIN WAS CRYSTALLIZED FROM 20% PEG MME 5K, 0.1 M MALONIC ACID, 0.1 M MES BUFFER PH 5.5, 0.02 M DTT, 0.01 M MANGANESE CHLORIDE
Resolution 2.95 Å R-free 0.235
1AB5 STRUCTURE OF CHEY MUTANT F14N, V21T Deposited 1997-02-04 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 4–128（125 aa）
Mutation：F14N, V21T No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.2；PROTEIN WAS CRYSTALLIZED FROM AMMONIUM SULFATE, 2.6-3.0M IN TRIS-HCL BUFFER AT PH 7.2.
Resolution 2.40 Å R-free 0.264
1AB5 STRUCTURE OF CHEY MUTANT F14N, V21T Deposited 1997-02-04 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 4–128（125 aa）
Mutation：F14N, V21T No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.2；PROTEIN WAS CRYSTALLIZED FROM AMMONIUM SULFATE, 2.6-3.0M IN TRIS-HCL BUFFER AT PH 7.2.
Resolution 2.40 Å R-free 0.264
1AB6 STRUCTURE OF CHEY MUTANT F14N, V86T Deposited 1997-02-04 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 4–128（125 aa）
Mutation：F14N, V86T No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.2；293 K；THE PROTEIN WAS CRYSTALLIZED WITH 3.0M AMMONIUM SULFATE, PH7.2 AT 20C., temperature 293K
Resolution 2.20 Å R-free 0.280
1AB6 STRUCTURE OF CHEY MUTANT F14N, V86T Deposited 1997-02-04 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 4–128（125 aa）
Mutation：F14N, V86T No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.2；293 K；THE PROTEIN WAS CRYSTALLIZED WITH 3.0M AMMONIUM SULFATE, PH7.2 AT 20C., temperature 293K
Resolution 2.20 Å R-free 0.280
1BDJ COMPLEX STRUCTURE OF HPT DOMAIN AND CHEY Deposited 1998-05-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–128（128 aa）
Not recorded SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；pH 7.0
Resolution 2.68 Å R-free 0.250
1C4W 1.9 A STRUCTURE OF A-THIOPHOSPHONATE MODIFIED CHEY D57C Deposited 1999-09-28 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–129（128 aa）
Mutation：D57(CYQ) Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 6.9；298 K；PEG 6000, SODIUM ACETATE, PIPES, pH 6.9, VAPOR DIFFUSION/HANGING DROP, temperature 298K
Resolution 1.84 Å
1CEY ASSIGNMENTS, SECONDARY STRUCTURE, GLOBAL FOLD, AND DYNAMICS OF CHEMOTAXIS Y PROTEIN USING THREE-AND FOUR-DIMENSIONAL HETERONUCLEAR (13C,15N) NMR SPECTROSCOPY Deposited 1994-11-23 Different construct Different mutation/modification Different oligomeric state Different experimental method Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–128（128 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR mmCIF provides none of the parsed conditions Resolution not provided
1CHN MAGNESIUM BINDING TO THE BACTERIAL CHEMOTAXIS PROTEIN CHEY RESULTS IN LARGE CONFORMATIONAL CHANGES INVOLVING ITS FUNCTIONAL SURFACE Deposited 1994-04-20 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.76 Å
1CYE THREE DIMENSIONAL STRUCTURE OF CHEMOTACTIC CHE Y PROTEIN IN AQUEOUS SOLUTION BY NUCLEAR MAGNETIC RESONANCE METHODS Deposited 1994-10-21 Different construct Different mutation/modification Different oligomeric state Different experimental method Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–128（127 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR mmCIF provides none of the parsed conditions Resolution not provided
1D4Z CRYSTAL STRUCTURE OF CHEY-95IV, A HYPERACTIVE CHEY MUTANT Deposited 1999-10-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–128（128 aa）
Mutation：I95V SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 4.8；278 K；AMMONIUM SULPHATE, PEG4000, SODIUM ACETATE, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 278K
Resolution 1.90 Å R-free 0.227
1DJM SOLUTION STRUCTURE OF BEF3-ACTIVATED CHEY FROM ESCHERICHIA COLI Deposited 1999-12-03 Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–129（129 aa）
Not recorded No recorded non-water small molecule SOLUTION NMR
NMR measurement conditions pH 6.7；298 K；Ionic strength (raw mmCIF value) 16 mM BECL2, 100 mM NAF, 20 mM MGCL2；Pressure AMBIENT
NMR measurement conditions pH 6.7；298 K；Ionic strength (raw mmCIF value) 16 mM BECL2, 100 mM NAF, 20 mM MGCL2；Pressure AMBIENT
NMR measurement conditions pH 6.7；298 K；Ionic strength (raw mmCIF value) 16 mM BECL2, 100 mM NAF, 20 mM MGCL2；Pressure AMBIENT
NMR sample composition 4 MM CHEY U-15N, 13C, 16 MM BECL2, 100 MM NAF, 20 MM MGCL2, PH 6.7
NMR sample composition 3 MM U-15N, 16 MM BECL2, 100 MM NAF, 20 MM MGCL2, PH 6.7
NMR sample composition 3 MM U-10% 13C, 16 MM BECL2, 100 MM NAF, 20 MM MGCL2, PH 6.7
NMR sample composition 2 MM CHEY, 16 MM BECL2, 100 MM NAF, 20 MM MGCL2, PH 6.7
Resolution not provided
1EAY CHEY-BINDING (P2) DOMAIN OF CHEA IN COMPLEX WITH CHEY FROM ESCHERICHIA COLI Deposited 1998-04-23 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–128（128 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；PROTEIN WAS CRYSTALLIZED FROM .63 M NAH2PO4/1.17 M K2HPO4, 10 MM NH4CL, PH 7.0
Resolution 2.00 Å
1EAY CHEY-BINDING (P2) DOMAIN OF CHEA IN COMPLEX WITH CHEY FROM ESCHERICHIA COLI Deposited 1998-04-23 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–128（128 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7；PROTEIN WAS CRYSTALLIZED FROM .63 M NAH2PO4/1.17 M K2HPO4, 10 MM NH4CL, PH 7.0
Resolution 2.00 Å
1EHC STRUCTURE OF SIGNAL TRANSDUCTION PROTEIN CHEY Deposited 1996-03-05 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–128（128 aa）
Mutation：D13K SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 8.3；pH 8.3
Resolution 2.26 Å
1F4V CRYSTAL STRUCTURE OF ACTIVATED CHEY BOUND TO THE N-TERMINUS OF FLIM Deposited 2000-06-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；ammonium sulfate, glycerol, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.22 Å R-free 0.258
1F4V CRYSTAL STRUCTURE OF ACTIVATED CHEY BOUND TO THE N-TERMINUS OF FLIM Deposited 2000-06-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 GOL GLYCEROL × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；ammonium sulfate, glycerol, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.22 Å R-free 0.258
1F4V CRYSTAL STRUCTURE OF ACTIVATED CHEY BOUND TO THE N-TERMINUS OF FLIM Deposited 2000-06-10 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1–128（128 aa）
Not recorded MG MAGNESIUM ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；ammonium sulfate, glycerol, Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.22 Å R-free 0.258
1FFG CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY AT 2.1 A RESOLUTION Deposited 2000-07-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–128（128 aa）
Not recorded MN MANGANESE (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；297 K；20% PEG MME 5K, 0.1 M MALONIC ACID, 0.1 M MES BUFFER 0.02 M DTT, 0.01 M MANGANESE CHLORIDE, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K
Resolution 2.10 Å R-free 0.220
1FFG CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY AT 2.1 A RESOLUTION Deposited 2000-07-25 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1–128（128 aa）
Not recorded MN MANGANESE (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；297 K；20% PEG MME 5K, 0.1 M MALONIC ACID, 0.1 M MES BUFFER 0.02 M DTT, 0.01 M MANGANESE CHLORIDE, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K
Resolution 2.10 Å R-free 0.220
1FFS CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY FROM CRYSTALS SOAKED IN ACETYL PHOSPHATE Deposited 2000-07-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–128（128 aa） Fragment：RESIDUES 124-257
Not recorded MN MANGANESE (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；297 K；20% PEG MME 5K, 0.1 M MALONIC ACID, 0.1 M MES BUFFER 0.02 M DTT, 0.01 M MANGANESE CHLORIDE , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K
Resolution 2.40 Å R-free 0.255
1FFS CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY FROM CRYSTALS SOAKED IN ACETYL PHOSPHATE Deposited 2000-07-26 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain C 1–128（128 aa） Fragment：RESIDUES 124-257
Not recorded MN MANGANESE (II) ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 5.5；297 K；20% PEG MME 5K, 0.1 M MALONIC ACID, 0.1 M MES BUFFER 0.02 M DTT, 0.01 M MANGANESE CHLORIDE , pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 297K
Resolution 2.40 Å R-free 0.255
1FQW CRYSTAL STRUCTURE OF ACTIVATED CHEY Deposited 2000-09-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–128（128 aa）
Not recorded MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；Ammonium sulfate, Tris buffer, MnCl2, glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.37 Å R-free 0.240
1FQW CRYSTAL STRUCTURE OF ACTIVATED CHEY Deposited 2000-09-07 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–128（128 aa）
Not recorded MN MANGANESE (II) ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；298 K；Ammonium sulfate, Tris buffer, MnCl2, glycerol, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.37 Å R-free 0.240
1HEY INVESTIGATING THE STRUCTURAL DETERMINANTS OF THE P21-LIKE TRIPHOSPHATE AND MG2+ BINDING SITE Deposited 1995-04-07 Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–128（128 aa）
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 2.24 Å
1KMI CRYSTAL STRUCTURE OF AN E.COLI CHEMOTAXIS PROTEIN, CHEZ Deposited 2001-12-16 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric
Chain Y 0–128（129 aa）
Not recorded MG MAGNESIUM ION × 2 BEF BERYLLIUM TRIFLUORIDE ION × 2 BCN BICINE × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；277 K；isopropanol, ammonium acetate, bicine, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K
Resolution 2.90 Å R-free 0.298
1MIH A ROLE FOR CHEY GLU 89 IN CHEZ-MEDIATED DEPHOSPHORYLATION OF THE E. COLI CHEMOTAXIS RESPONSE REGULATOR CHEY Deposited 2002-08-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 0–128（129 aa）
Mutation：N59R MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 1 BEF BERYLLIUM TRIFLUORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；293 K；1.8 M Ammonium sulfate, 1 mM MnCl2, 10 mM NaF, 1 mM BeCl2, 5% glycerol, 10 mg/ml CheY N59R, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.277
1MIH A ROLE FOR CHEY GLU 89 IN CHEZ-MEDIATED DEPHOSPHORYLATION OF THE E. COLI CHEMOTAXIS RESPONSE REGULATOR CHEY Deposited 2002-08-23 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 0–128（129 aa）
Mutation：N59R MN MANGANESE (II) ION × 1 SO4 SULFATE ION × 2 BEF BERYLLIUM TRIFLUORIDE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；293 K；1.8 M Ammonium sulfate, 1 mM MnCl2, 10 mM NaF, 1 mM BeCl2, 5% glycerol, 10 mg/ml CheY N59R, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K
Resolution 2.70 Å R-free 0.277
1U8T Crystal structure of CheY D13K Y106W alone and in complex with a FliM peptide Deposited 2004-08-06 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain A 1–128（128 aa）
Mutation：D13K Y106W Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.9；277 K；PEG 3350, ammonium sulfate, Tris, VAPOR DIFFUSION, temperature 277K, pH 7.90
Resolution 1.50 Å R-free 0.273
1U8T Crystal structure of CheY D13K Y106W alone and in complex with a FliM peptide Deposited 2004-08-06 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric
Chain B 1–128（128 aa）
Mutation：D13K Y106W Non-standard monomer：Yes (specific site not provided by mmCIF) No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.9；277 K；PEG 3350, ammonium sulfate, Tris, VAPOR DIFFUSION, temperature 277K, pH 7.90
Resolution 1.50 Å R-free 0.273
1U8T Crystal structure of CheY D13K Y106W alone and in complex with a FliM peptide Deposited 2004-08-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain C 1–128（128 aa）
Mutation：D13K Y106W Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.9；277 K；PEG 3350, ammonium sulfate, Tris, VAPOR DIFFUSION, temperature 277K, pH 7.90
Resolution 1.50 Å R-free 0.273
1U8T Crystal structure of CheY D13K Y106W alone and in complex with a FliM peptide Deposited 2004-08-06 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain D 1–128（128 aa）
Mutation：D13K Y106W Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.9；277 K；PEG 3350, ammonium sulfate, Tris, VAPOR DIFFUSION, temperature 277K, pH 7.90
Resolution 1.50 Å R-free 0.273
1U8T Crystal structure of CheY D13K Y106W alone and in complex with a FliM peptide Deposited 2004-08-06 Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 5 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain C 1–128（128 aa）
Chain D 1–128（128 aa）
Mutation：D13K Y106W Non-standard monomer：Yes (specific site not provided by mmCIF) Mutation：D13K Y106W Non-standard monomer：Yes (specific site not provided by mmCIF) SO4 SULFATE ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.9；277 K；PEG 3350, ammonium sulfate, Tris, VAPOR DIFFUSION, temperature 277K, pH 7.90
Resolution 1.50 Å R-free 0.273
1UDR CHEY MUTANT WITH LYS 91 REPLACED BY ASP, LYS 92 REPLACED BY ALA, ILE 96 REPLACED BY LYS AND ALA 98 REPLACED BY LEU (STABILIZING MUTATIONS IN HELIX 4) Deposited 1996-11-05 Different construct Different mutation/modification Different oligomeric state Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 4 PDB declaration: tetrameric
Chain A 2–128（127 aa）
Chain B 2–128（127 aa）
Chain C 2–128（127 aa）
Chain D 2–128（127 aa）
Mutation：K91D, K92A, I96K, A98L Mutation：K91D, K92A, I96K, A98L Mutation：K91D, K92A, I96K, A98L Mutation：K91D, K92A, I96K, A98L No recorded non-water small molecule X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.90 Å
1YMU SIGNAL TRANSDUCTION PROTEIN CHEY MUTANT WITH MET 17 REPLACED BY GLY (M17G) Deposited 1995-12-14 Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics Assembly 1 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain A 2–128（127 aa）
Chain B 2–128（127 aa）
Mutation：INS(M0), M1R, A2S, M17G Mutation：INS(M0), M1R, A2S, M17G No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions pH 7.2；pH 7.2
Resolution 2.30 Å
1YMV SIGNAL TRANSDUCTION PROTEIN CHEY MUTANT WITH PHE 14 REPLACED BY GLY, SER 15 REPLACED BY GLY, AND MET 17 REPLACED BY GLY Deposited 1995-12-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 2–128（127 aa）
Mutation：INS(M0), M1R, A2S, F14G, S15G, M17G MG MAGNESIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6.8；pH 6.8
Resolution 1.90 Å
1ZDM Crystal Structure of Activated CheY Bound to Xe Deposited 2005-04-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 0–128（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MN MANGANESE (II) ION × 1 XE XENON × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；1.8 M ammonium sulfate, 5-10% glycerol, 100 mM Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.40 Å R-free 0.267
1ZDM Crystal Structure of Activated CheY Bound to Xe Deposited 2005-04-14 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 0–128（129 aa）
Non-standard monomer：Yes (specific site not provided by mmCIF) MN MANGANESE (II) ION × 1 XE XENON × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.4；298 K；1.8 M ammonium sulfate, 5-10% glycerol, 100 mM Tris, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K
Resolution 2.40 Å R-free 0.267
3CHY CRYSTAL STRUCTURE OF ESCHERICHIA COLI CHEY REFINED AT 1.7-ANGSTROM RESOLUTION Deposited 1991-04-22 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–128（128 aa）
Not recorded SO4 SULFATE ION × 3 X-RAY DIFFRACTION mmCIF provides none of the parsed conditions Resolution 1.66 Å
5CHY STRUCTURE OF CHEMOTAXIS PROTEIN CHEY Deposited 1996-08-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–128（128 aa）
Mutation：Y106W CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions pH 6；28% PEG 8000, 0.12 M CALCIUM ACETATE, 0.1 M CACODYLATE BUFFER, PH 6.0
Resolution 2.00 Å
6CHY STRUCTURE OF CHEMOTAXIS PROTEIN CHEY Deposited 1996-08-29 Different construct Different mutation/modification Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–128（128 aa）
Mutation：T87I, Y106W No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions 18% PEG 3350 AND 0.2 M AMMONIUM SULFATE
Resolution 2.33 Å
6CHY STRUCTURE OF CHEMOTAXIS PROTEIN CHEY Deposited 1996-08-29 Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain B 1–128（128 aa）
Mutation：T87I, Y106W SO4 SULFATE ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions 18% PEG 3350 AND 0.2 M AMMONIUM SULFATE
Resolution 2.33 Å
6CHY STRUCTURE OF CHEMOTAXIS PROTEIN CHEY Deposited 1996-08-29 Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein homooligomer Homooligomer；Protein × 2 PDB declaration: dimeric
Chain B 1–128（128 aa）
Mutation：T87I, Y106W SO4 SULFATE ION × 2 X-RAY DIFFRACTION
X-ray crystallization conditions 18% PEG 3350 AND 0.2 M AMMONIUM SULFATE
Resolution 2.33 Å

27 other PDB entries and 46 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name CHEY_ECOLI
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 1–128； UniProt 1–128 Author chain B； PDBConstruct 1–128； UniProt 1–128

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 1vlz

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 1vlz
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2. Structure Basics 2. Structure Basics

Entry ID entry_id1vlz
Deposition date deposition_date1995-04-21
Structure title titleUNCOUPLED PHOSPHORYLATION AND ACTIVATION IN BACTERIAL CHEMOTAXIS: THE 2.1 ANGSTROM STRUCTURE OF A THREONINE TO ISOLEUCINE MUTANT AT POSITION 87 OF CHEY
Keywords keywordsCHEY, RESPONSE REGULATORS, TWO-COMPONENT SYSTEMS, SIGNAL TRANSDUCTION PROTEIN; SIGNAL TRANSDUCTION PROTEIN
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier20.76 Å
Radius of gyration Rg (electron density) rg_electron19.79 Å
Forward intensity I(0) i012267200.00
Molecular weight molecular_weight27043.0 kDa
Excluded volume excluded_volume34224 ų
Envelope volume envelope_volume40582 ų
Hydration-shell volume shell_volume17707 ų
Envelope diameter envelope_diameter66.9 Å
Shell Rg shell_rg25.26 Å
Envelope Rg envelope_rg19.78 Å
Shape Rg shape_rg19.81 Å
Total Rg total_rg20.55 Å
Total atoms total_atoms1898
Residues n_residues255
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax68.2 Å
Rg (real space) rg_real20.76 Å
Rg uncertainty (real space) rg_real_error0.49 Å
I(0) (real space) i0_real1.2270e+07
I(0) uncertainty (real space) i0_real_error1.6090e+05
Rg (reciprocal space) rg_reciprocal20.76 Å
I(0) (reciprocal space) i0_reciprocal12270000.0000
Solution quality estimate total_estimate0.8037
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks2
Primary peak position r_peak_primary22.7 Å
Skewness Skewness skewness0.334
Kurtosis Kurtosis kurtosis-0.502
Angular range angular_range— – 0.3850 Å−1
Current regularization parameter α current_alpha0.0000
Highest regularization parameter α highest_alpha3191000.0000
Real-space data points n_real_points70
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.822; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.979; Smooth: 0.000
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (2)

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7. Fold Classification (SCOP + CATH) 4 domains

SCOP 2.08 (2 domains)

Domain ID domain_idd1vlza_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.23 — Flavodoxin-like
Superfamily Superfamily superfamilyc.23.1 — CheY-like
Family Family familyc.23.1.1 — CheY-related
Domain ID domain_idd1vlzb_
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.23 — Flavodoxin-like
Superfamily Superfamily superfamilyc.23.1 — CheY-like
Family Family familyc.23.1.1 — CheY-related

CATH v4.4 (2 domains)

Domain ID domain_id1vlzA00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily2300 — Response regulator
Domain ID domain_id1vlzB00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily2300 — Response regulator
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8. Citations (3)

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9. Files and Curves (10)