1on1

Bacillus Subtilis Manganese Transport Regulator (Mntr) Bound To Manganese, AB Conformation.

Method: X-RAY DIFFRACTION Dmax: 63.8 Å Quality: EXCELLENT

SAXS 散射曲线 SAXS Profile

SAXS profile for 1on1

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1on1
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1on1
沉积日期 deposition_date2003-02-26
结构标题 titleBacillus Subtilis Manganese Transport Regulator (Mntr) Bound To Manganese, AB Conformation.
关键词 keywordsHelix-turn-helix, DNA-Binding Protein, Metalloregulatory Protein, TRANSCRIPTION; TRANSCRIPTION
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier21.64
回转半径 Rg (电子) rg_electron20.93
零角强度 I(0) i015683500.00
分子量 molecular_weight30458.0 kDa
排除体积 excluded_volume38368 ų
包络体积 envelope_volume46529 ų
水化壳体积 shell_volume19114 ų
包络直径 envelope_diameter65.2
壳层 Rg shell_rg26.59
包络 Rg envelope_rg21.04
形状 Rg shape_rg20.93
总 Rg total_rg21.74
总原子数 total_atoms2134
残基数 n_residues260
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax63.8
Rg (实空间) rg_real21.55
Rg 误差 (实空间) rg_real_error0.35
I(0) (实空间) i0_real1.5680e+07
I(0) 误差 (实空间) i0_real_error1.8120e+05
Rg (倒空间) rg_reciprocal21.57
I(0) (倒空间) i0_reciprocal15680000.0000
解质量估计 total_estimate0.9191
解质量评级 solution_quality EXCELLENT a EXCELLENT solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary22.8
偏度 Skewness skewness0.141
峰度 Kurtosis kurtosis-0.688
角度范围 angular_range— – 0.3650 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha2212000.0000
实空间数据点数 n_real_points68
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.985; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.990

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (3)

6. 折叠分类 (SCOP + CATH) 8 domains

SCOP 2.08 (4 domains)

结构域编号 domain_idd1on1a1
类 Class classa — All alpha proteins
折叠类型 Fold folda.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamilya.4.5 — 'Winged helix' DNA-binding domain
家族 Family familya.4.5.24 — Iron-dependent repressor protein
结构域编号 domain_idd1on1a2
类 Class classa — All alpha proteins
折叠类型 Fold folda.76 — Iron-dependent repressor protein, dimerization domain
超家族 Superfamily superfamilya.76.1 — Iron-dependent repressor protein, dimerization domain
家族 Family familya.76.1.1 — Iron-dependent repressor protein, dimerization domain
结构域编号 domain_idd1on1b1
类 Class classa — All alpha proteins
折叠类型 Fold folda.4 — DNA/RNA-binding 3-helical bundle
超家族 Superfamily superfamilya.4.5 — 'Winged helix' DNA-binding domain
家族 Family familya.4.5.24 — Iron-dependent repressor protein
结构域编号 domain_idd1on1b2
类 Class classa — All alpha proteins
折叠类型 Fold folda.76 — Iron-dependent repressor protein, dimerization domain
超家族 Superfamily superfamilya.76.1 — Iron-dependent repressor protein, dimerization domain
家族 Family familya.76.1.1 — Iron-dependent repressor protein, dimerization domain

CATH v4.4 (4 domains)

结构域编号 domain_id1on1A01
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
结构域编号 domain_id1on1A02
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology60 — Diphtheria Toxin Repressor; domain 2
同源超家族 H-superfamily homologous superfamily10 — Iron dependent repressor, metal binding and dimerisation domain
结构域编号 domain_id1on1B01
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology10 — Arc Repressor Mutant, subunit A
同源超家族 H-superfamily homologous superfamily10 — Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
结构域编号 domain_id1on1B02
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology60 — Diphtheria Toxin Repressor; domain 2
同源超家族 H-superfamily homologous superfamily10 — Iron dependent repressor, metal binding and dimerisation domain

7. 引用文献 (1)

8. 文件与曲线 (10)