1pv4

X-ray crystal structure of the Rho transcription termination factor in complex with single stranded DNA

Method: X-RAY DIFFRACTION Dmax: 149.2 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1pv4

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1pv4
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1pv4
沉积日期 deposition_date2003-06-26
结构标题 titleX-ray crystal structure of the Rho transcription termination factor in complex with single stranded DNA
关键词 keywordsPROTEIN-ssDNA COMPLEX, Transcription-DNA COMPLEX; Transcription/DNA
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier46.94
回转半径 Rg (电子) rg_electron46.20
零角强度 I(0) i01151420000.00
分子量 molecular_weight275900.0 kDa
排除体积 excluded_volume341910 ų
包络体积 envelope_volume487760 ų
水化壳体积 shell_volume84092 ų
包络直径 envelope_diameter152.8
壳层 Rg shell_rg54.93
包络 Rg envelope_rg45.04
形状 Rg shape_rg46.16
总 Rg total_rg46.64
总原子数 total_atoms19036
残基数 n_residues2314
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax149.2
Rg (实空间) rg_real46.65
Rg 误差 (实空间) rg_real_error1.04
I(0) (实空间) i0_real1.1510e+09
I(0) 误差 (实空间) i0_real_error2.1000e+07
Rg (倒空间) rg_reciprocal46.94
I(0) (倒空间) i0_reciprocal1152000000.0000
解质量估计 total_estimate0.8948
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary65.8
偏度 Skewness skewness0.052
峰度 Kurtosis kurtosis-0.609
角度范围 angular_range— – 0.1700 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha128400000.0000
实空间数据点数 n_real_points35
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.907; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.978; Smooth: 0.929

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (3)

6. 折叠分类 (SCOP + CATH) 29 domains

SCOP 2.08 (17 domains)

结构域编号 domain_idd1pv4a1
类 Class classa — All alpha proteins
折叠类型 Fold folda.140 — LEM/SAP HeH motif
超家族 Superfamily superfamilya.140.3 — Rho N-terminal domain-like
家族 Family familya.140.3.1 — Rho termination factor, N-terminal domain
结构域编号 domain_idd1pv4a2
类 Class classb — All beta proteins
折叠类型 Fold foldb.40 — OB-fold
超家族 Superfamily superfamilyb.40.4 — Nucleic acid-binding proteins
家族 Family familyb.40.4.5 — Cold shock DNA-binding domain-like
结构域编号 domain_idd1pv4a3
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family familyc.37.1.11 — RecA protein-like (ATPase-domain)
结构域编号 domain_idd1pv4b1
类 Class classb — All beta proteins
折叠类型 Fold foldb.40 — OB-fold
超家族 Superfamily superfamilyb.40.4 — Nucleic acid-binding proteins
家族 Family familyb.40.4.5 — Cold shock DNA-binding domain-like
结构域编号 domain_idd1pv4b2
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family familyc.37.1.11 — RecA protein-like (ATPase-domain)
结构域编号 domain_idd1pv4c1
类 Class classa — All alpha proteins
折叠类型 Fold folda.140 — LEM/SAP HeH motif
超家族 Superfamily superfamilya.140.3 — Rho N-terminal domain-like
家族 Family familya.140.3.1 — Rho termination factor, N-terminal domain
结构域编号 domain_idd1pv4c2
类 Class classb — All beta proteins
折叠类型 Fold foldb.40 — OB-fold
超家族 Superfamily superfamilyb.40.4 — Nucleic acid-binding proteins
家族 Family familyb.40.4.5 — Cold shock DNA-binding domain-like
结构域编号 domain_idd1pv4c3
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family familyc.37.1.11 — RecA protein-like (ATPase-domain)
结构域编号 domain_idd1pv4d1
类 Class classa — All alpha proteins
折叠类型 Fold folda.140 — LEM/SAP HeH motif
超家族 Superfamily superfamilya.140.3 — Rho N-terminal domain-like
家族 Family familya.140.3.1 — Rho termination factor, N-terminal domain
结构域编号 domain_idd1pv4d2
类 Class classb — All beta proteins
折叠类型 Fold foldb.40 — OB-fold
超家族 Superfamily superfamilyb.40.4 — Nucleic acid-binding proteins
家族 Family familyb.40.4.5 — Cold shock DNA-binding domain-like
结构域编号 domain_idd1pv4d3
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family familyc.37.1.11 — RecA protein-like (ATPase-domain)
结构域编号 domain_idd1pv4e1
类 Class classa — All alpha proteins
折叠类型 Fold folda.140 — LEM/SAP HeH motif
超家族 Superfamily superfamilya.140.3 — Rho N-terminal domain-like
家族 Family familya.140.3.1 — Rho termination factor, N-terminal domain
结构域编号 domain_idd1pv4e2
类 Class classb — All beta proteins
折叠类型 Fold foldb.40 — OB-fold
超家族 Superfamily superfamilyb.40.4 — Nucleic acid-binding proteins
家族 Family familyb.40.4.5 — Cold shock DNA-binding domain-like
结构域编号 domain_idd1pv4e3
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family familyc.37.1.11 — RecA protein-like (ATPase-domain)
结构域编号 domain_idd1pv4f1
类 Class classa — All alpha proteins
折叠类型 Fold folda.140 — LEM/SAP HeH motif
超家族 Superfamily superfamilya.140.3 — Rho N-terminal domain-like
家族 Family familya.140.3.1 — Rho termination factor, N-terminal domain
结构域编号 domain_idd1pv4f2
类 Class classb — All beta proteins
折叠类型 Fold foldb.40 — OB-fold
超家族 Superfamily superfamilyb.40.4 — Nucleic acid-binding proteins
家族 Family familyb.40.4.5 — Cold shock DNA-binding domain-like
结构域编号 domain_idd1pv4f3
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.37 — P-loop containing nucleoside triphosphate hydrolases
超家族 Superfamily superfamilyc.37.1 — P-loop containing nucleoside triphosphate hydrolases
家族 Family familyc.37.1.11 — RecA protein-like (ATPase-domain)

CATH v4.4 (12 domains)

结构域编号 domain_id1pv4A01
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily140 — Nucleic acid-binding proteins
结构域编号 domain_id1pv4A02
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
结构域编号 domain_id1pv4B01
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily140 — Nucleic acid-binding proteins
结构域编号 domain_id1pv4B02
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
结构域编号 domain_id1pv4C01
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily140 — Nucleic acid-binding proteins
结构域编号 domain_id1pv4C02
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
结构域编号 domain_id1pv4D01
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily140 — Nucleic acid-binding proteins
结构域编号 domain_id1pv4D02
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
结构域编号 domain_id1pv4E01
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily140 — Nucleic acid-binding proteins
结构域编号 domain_id1pv4E02
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases
结构域编号 domain_id1pv4F01
类 Class class2 — Mainly Beta
架构 Architecture architecture40 — Beta Barrel
拓扑 Topology topology50 — OB fold (Dihydrolipoamide Acetyltransferase, E2P)
同源超家族 H-superfamily homologous superfamily140 — Nucleic acid-binding proteins
结构域编号 domain_id1pv4F02
类 Class class3 — Alpha Beta
架构 Architecture architecture40 — 3-Layer(aba) Sandwich
拓扑 Topology topology50 — Rossmann fold
同源超家族 H-superfamily homologous superfamily300 — P-loop containing nucleotide triphosphate hydrolases

7. 引用文献 (1)

8. 文件与曲线 (10)