1rco

SPINACH RUBISCO IN COMPLEX WITH THE INHIBITOR D-XYLULOSE-2,2-DIOL-1,5-BISPHOSPHATE

Method: X-RAY DIFFRACTION Dmax: 135.8 Å Quality: GOOD

1. 蛋白身份与相关结构 Protein Identity & Related Structures

RIBULOSE BISPHOSPHATE CARBOXYLASE/OXYGENASE

物种未注明

UniProt P00875

当前结构中的状态

Assembly 聚集状态 构建体 突变与修饰 配体、离子与共同组分 实验方法与环境 结构质量
1 蛋白异源复合物 异源复合物 蛋白 × 16 PDB 声明:hexadecameric(16) 与蛋白拷贝数一致 链 B; UniProt 1–475 链 E; UniProt 1–475 链 H; UniProt 1–475 链 K; UniProt 1–475 链 L; UniProt 1–475 链 O; UniProt 1–475 链 R; UniProt 1–475 链 V; UniProt 1–475 未记录 RIBULOSE BISPHOSPHATE CARBOXYLASE/OXYGENASE × 8 (P00870) XDP D-XYLULOSE-2,2-DIOL-1,5-BISPHOSPHATE × 8 X-RAY DIFFRACTION X-ray结晶条件:pH 7.8;15% PEG 4000, 25 MM HEPES PH 7.8, 10 MM CACL2, 0.2 M NACL, 50 MM NAHCO3, 20 MM XUBP 分辨率 2.30 Å R-free 0.246

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly, “相对当前条目”直接指出证据层面的不同;没有差异标签表示当前已读取字段一致。

共 11 个其他 PDB 条目、11 个 assembly。 打开独立比较页并筛选聚集状态

查看构建体与数据证据
UniProt名称 RBL_SPIOL
Isoform
PDB实体 1
链与序列区间 作者链 B; PDB构建体 1–475; UniProt 1–475 作者链 E; PDB构建体 1–475; UniProt 1–475 作者链 H; PDB构建体 1–475; UniProt 1–475 作者链 K; PDB构建体 1–475; UniProt 1–475 作者链 L; PDB构建体 1–475; UniProt 1–475 作者链 O; PDB构建体 1–475; UniProt 1–475 作者链 R; PDB构建体 1–475; UniProt 1–475 作者链 V; PDB构建体 1–475; UniProt 1–475

RIBULOSE BISPHOSPHATE CARBOXYLASE/OXYGENASE

物种未注明

UniProt P00870

当前结构中的状态

Assembly 聚集状态 构建体 突变与修饰 配体、离子与共同组分 实验方法与环境 结构质量
1 蛋白异源复合物 异源复合物 蛋白 × 16 PDB 声明:hexadecameric(16) 与蛋白拷贝数一致 链 C; UniProt 58–180 链 F; UniProt 58–180 链 I; UniProt 58–180 链 M; UniProt 58–180 链 P; UniProt 58–180 链 S; UniProt 58–180 链 T; UniProt 58–180 链 W; UniProt 58–180 未记录 RIBULOSE BISPHOSPHATE CARBOXYLASE/OXYGENASE × 8 (P00875) XDP D-XYLULOSE-2,2-DIOL-1,5-BISPHOSPHATE × 8 X-RAY DIFFRACTION X-ray结晶条件:pH 7.8;15% PEG 4000, 25 MM HEPES PH 7.8, 10 MM CACL2, 0.2 M NACL, 50 MM NAHCO3, 20 MM XUBP 分辨率 2.30 Å R-free 0.246

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly, “相对当前条目”直接指出证据层面的不同;没有差异标签表示当前已读取字段一致。

共 4 个其他 PDB 条目、4 个 assembly。 打开独立比较页并筛选聚集状态

查看构建体与数据证据
UniProt名称 RBS1_SPIOL
Isoform
PDB实体 2
链与序列区间 作者链 C; PDB构建体 1–123; UniProt 58–180 作者链 F; PDB构建体 1–123; UniProt 58–180 作者链 I; PDB构建体 1–123; UniProt 58–180 作者链 M; PDB构建体 1–123; UniProt 58–180 作者链 P; PDB构建体 1–123; UniProt 58–180 作者链 S; PDB构建体 1–123; UniProt 58–180 作者链 T; PDB构建体 1–123; UniProt 58–180 作者链 W; PDB构建体 1–123; UniProt 58–180

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。 链映射与序列区间收在“数据证据”中;数据库内部编号、导入时间和 assembly 操作表达式仅用于维护,因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

SAXS profile for 1rco

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1rco
下载 Download

2. 结构基本信息 2. Structure Basics

条目编号 entry_id1rco
沉积日期 deposition_date1996-10-31
结构标题 titleSPINACH RUBISCO IN COMPLEX WITH THE INHIBITOR D-XYLULOSE-2,2-DIOL-1,5-BISPHOSPHATE
关键词 keywordsLYASE, CARBON-CARBON; LYASE
实验方法 methodX-RAY DIFFRACTION

3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier47.24
回转半径 Rg (电子) rg_electron46.53
零角强度 I(0) i04043900000.00
分子量 molecular_weight533660.0 kDa
排除体积 excluded_volume666530 ų
包络体积 envelope_volume804240 ų
水化壳体积 shell_volume129760 ų
包络直径 envelope_diameter137.6
壳层 Rg shell_rg59.98
包络 Rg envelope_rg46.22
形状 Rg shape_rg46.53
总 Rg total_rg46.90
总原子数 total_atoms37608
残基数 n_residues4720
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax135.8
Rg (实空间) rg_real46.76
Rg 误差 (实空间) rg_real_error0.57
I(0) (实空间) i0_real4.0440e+09
I(0) 误差 (实空间) i0_real_error6.8960e+07
Rg (倒空间) rg_reciprocal47.23
I(0) (倒空间) i0_reciprocal4046000000.0000
解质量估计 total_estimate0.8724
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary65.8
偏度 Skewness skewness-0.027
峰度 Kurtosis kurtosis-0.578
角度范围 angular_range— – 0.1650 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha2880000000.0000
实空间数据点数 n_real_points34
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.945; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.951; Smooth: 0.552

5. 晶体学与实验 5. Crystallography & Experiment

6. 实体与聚合物信息 Entities & Polymers (4)

7. 折叠分类 (SCOP + CATH) 48 domains

SCOP 2.08 (24 domains)

结构域编号 domain_idd1rcob1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1rcob2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.1 — Ribulose 1,5-bisphosphate carboxylase-oxygenase
结构域编号 domain_idd1rcoc_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1rcoe1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1rcoe2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.1 — Ribulose 1,5-bisphosphate carboxylase-oxygenase
结构域编号 domain_idd1rcof_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1rcoh1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1rcoh2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.1 — Ribulose 1,5-bisphosphate carboxylase-oxygenase
结构域编号 domain_idd1rcoi_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1rcok1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1rcok2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.1 — Ribulose 1,5-bisphosphate carboxylase-oxygenase
结构域编号 domain_idd1rcol1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1rcol2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.1 — Ribulose 1,5-bisphosphate carboxylase-oxygenase
结构域编号 domain_idd1rcom_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1rcoo1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1rcoo2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.1 — Ribulose 1,5-bisphosphate carboxylase-oxygenase
结构域编号 domain_idd1rcop_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1rcor1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1rcor2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.1 — Ribulose 1,5-bisphosphate carboxylase-oxygenase
结构域编号 domain_idd1rcos_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1rcot_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1rcov1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1rcov2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.1 — Ribulose 1,5-bisphosphate carboxylase-oxygenase
结构域编号 domain_idd1rcow_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit

CATH v4.4 (24 domains)

结构域编号 domain_id1rcoB01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1rcoB02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1rcoC00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1rcoE01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1rcoE02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1rcoF00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1rcoH01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1rcoH02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1rcoI00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1rcoK01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1rcoK02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1rcoL01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1rcoL02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1rcoM00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1rcoO01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1rcoO02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1rcoP00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1rcoR01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1rcoR02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1rcoS00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1rcoT00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1rcoV01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1rcoV02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1rcoW00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit

8. 引用文献 (2)

9. 文件与曲线 (10)