SAXSdb — 1t3l

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Dihydropyridine-sensitive L-type, calcium channel beta-2 subunit

Oryctolagus cuniculus

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count	Chain A； UniProt 25–422	Fragment：Functional Core (Residues 25-422) Mutation：P122R	Voltage-dependent L-type calcium channel alpha-1S subunit × 1 （P07293）	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；PEG 20K, Bicine, sodium chloride, beta-mercaptoethanol, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.20 Å R-free 0.282

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 1T3L	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
1T3S Structural Analysis of the Voltage-Dependent Calcium Channel Beta Subunit Functional Core Deposited 2004-04-27	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 25–137（113 aa） Fragment：Functional Core (Residues 25-422) Chain A 203–422（220 aa） Fragment：Functional Core (Residues 25-422)	Mutation：P122R Mutation：P122R	HG MERCURY (II) ION × 1	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 9；292 K；PEG 20K, BICINE, SODIUM CHLORIDE, BETA-MERCAPTOETHANOL, MPD, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 292K	Resolution 2.30 Å R-free 0.277
4DEX Crystal structure of the Voltage Dependent Calcium Channel beta-2 Subunit in Complex With The CaV2.2 I-II Linker. Deposited 2012-01-22	Different construct Different mutation/modification Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 51–163（113 aa） Chain A 229–448（220 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION；pH 8；292 K；0.1 M NaCl, 0.1 M Bicine, 23-26% PEG 400 , pH 8.0, VAPOR DIFFUSION, temperature 292K X-ray crystallization conditions VAPOR DIFFUSION；pH 8；292 K；0.1 M NaCl, 0.1 M Bicine, 23-26% PEG 400 Soaked with NaBr before freezing, pH 8.0, VAPOR DIFFUSION, temperature 292K X-ray crystallization conditions VAPOR DIFFUSION；pH 8；292 K；0.1 M NaCl, 0.1 M Bicine, 23-26% PEG 400 Soaked with NaBr before freezing, pH 8.0, VAPOR DIFFUSION, temperature 292K	Resolution 2.00 Å R-free 0.238
4DEY Crystal structure of the Voltage Dependent Calcium Channel beta-2 Subunit in Complex With The CaV1.2 I-II Linker. Deposited 2012-01-22	Different construct Different mutation/modification Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 51–163（113 aa） Chain A 229–448（220 aa）	Not recorded	BR BROMIDE ION × 17	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION；pH 7.5；292 K；1.1-1.25 M potassium sodium tartrate, 0.1 M Tris Soaked with KI before freezing , pH 7.5, VAPOR DIFFUSION, temperature 292K X-ray crystallization conditions VAPOR DIFFUSION；pH 7.5；292 K；1.1-1.25 M potassium sodium tartrate, 0.1 M Tris Soaked with KI before freezing, pH 7.5, VAPOR DIFFUSION, temperature 292K X-ray crystallization conditions VAPOR DIFFUSION；pH 7.5；292 K；1.1-1.25 M potassium sodium tartrate, 0.1 M Tris Soaked with NaBr before freezing, pH 7.5, VAPOR DIFFUSION, temperature 292K	Resolution 1.95 Å R-free 0.229

3 other PDB entries and 3 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	CACB2_RABIT
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–337； UniProt 25–422

Voltage-dependent L-type calcium channel alpha-1S subunit

OrganismNot specified

UniProt P07293

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein heterocomplex Heteromer Protein × 2 PDB declaration: dimeric（2） Consistent with protein copy count	Chain B； UniProt 357–374	Fragment：AID	Dihydropyridine-sensitive L-type, calcium channel beta-2 subunit × 1 （P54288）	X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, HANGING DROP；pH 9；293 K；PEG 20K, Bicine, sodium chloride, beta-mercaptoethanol, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 293K	Resolution 2.20 Å R-free 0.282

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 1T3L	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
1DU1 PEPTIDE FRAGMENT THR671-LEU690 OF THE RABBIT SKELETAL DIHYDROPYRIDINE RECEPTOR Deposited 2000-01-13	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 671–690（20 aa） Fragment：II-III LOOP REGION, FRAGMENT THR671-LEU690 A1	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 5；278 K；Ionic strength (raw mmCIF value) 0；Pressure 1 NMR sample composition 4 mM peptide, no buffer \| 90% H2O/10% D2O	Resolution not provided
3JBR Cryo-EM structure of the rabbit voltage-gated calcium channel Cav1.1 complex at 4.2 angstrom Deposited 2015-09-29	Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 4 PDB declaration: tetrameric	Chain A 1–1395（1395 aa） Chain A 1521–1873（353 aa）	Not recorded	CA CALCIUM ION × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 15 BMA beta-D-mannopyranose × 2	ELECTRON MICROSCOPY cryo-EM buffer 20mM MOPS, 200mM NaCl, 0.5mM CaCl2,0.1% digitonin；pH 7.4；20mM MOPS, 200mM NaCl, 0.5mM CaCl2,0.1% digitonin cryo-EM vitrification conditions Blot for 2 seconds before plunging；Cryogen ETHANE	Resolution 4.20 Å
5GJV Structure of the mammalian voltage-gated calcium channel Cav1.1 complex at near atomic resolution Deposited 2016-07-02	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric	Chain A 1–1873（1873 aa）	Not recorded	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 14 CA CALCIUM ION × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 8；100 mM Tris-HCl, pH 8.0,200 mM NaCl, 10 mM CaCl2,15 mM reduced glutathione, 0.1% digitonin, and protease inhibitors. cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2 s and flash-frozen in liquid ethane cooled by liquid nitrogen	Resolution 3.60 Å
5GJW Structure of the mammalian voltage-gated calcium channel Cav1.1 complex for ClassII map Deposited 2016-07-02	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric	Chain A 1–1873（1873 aa）	Not recorded	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 3	ELECTRON MICROSCOPY cryo-EM buffer pH 8；100 mM Tris-HCl, pH 8.0,200 mM NaCl, 10 mM CaCl2,15 mM reduced glutathione, 0.1% digitonin, and protease inhibitors. cryo-EM vitrification conditions Cryogen ETHANE；Grids were blotted for 2 s and flash-frozen in liquid ethane cooled by liquid nitrogen	Resolution 3.90 Å
6BYO Residue assignment correction to the voltage gated calcium Cav1.1 rabbit alpha 1 subunit PDB entries 3JBR & 5GJV Deposited 2017-12-21	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 32–1388（1357 aa）	Not recorded	No recorded non-water small molecule	ELECTRON MICROSCOPY cryo-EM buffer pH 6.5 cryo-EM vitrification conditions Cryogen NITROGEN	Resolution 3.60 Å
6JP5 Rabbit Cav1.1-Nifedipine Complex Deposited 2019-03-25	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric	Chain A 1–1873（1873 aa）	Not recorded	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 9 CA CALCIUM ION × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 C5U dimethyl 2,6-dimethyl-4-(2-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylate × 1 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 ETA ETHANOLAMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
6JP8 Rabbit Cav1.1-Bay K8644 Complex Deposited 2019-03-26	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric	Chain A 1–1873（1873 aa）	Not recorded	C8U methyl (4~{S})-2,6-dimethyl-5-nitro-4-[2-(trifluoromethyl)phenyl]-1,4-dihydropyridine-3-carboxylate × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 2 ETA ETHANOLAMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.70 Å
6JPA Rabbit Cav1.1-Verapamil Complex Deposited 2019-03-26	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric	Chain A 1–1506（1506 aa）	Not recorded	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 8 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 2 9Z9 (3beta,14beta,17beta,25R)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en × 1 4YH (2S)-2-(3,4-dimethoxyphenyl)-5-{[2-(3,4-dimethoxyphenyl)ethyl](methyl)amino}-2-(propan-2-yl)pentanenitrile × 2 ETA ETHANOLAMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.60 Å
6JPB Rabbit Cav1.1-Diltiazem Complex Deposited 2019-03-26	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 5 PDB declaration: pentameric	Chain A 1–1873（1873 aa）	Not recorded	NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 8 CA CALCIUM ION × 3 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 4 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 C9F Diltiazem × 1 ETA ETHANOLAMINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.90 Å
7JPK Rabbit Cav1.1 in the presence of 100 micromolar (S)-(-)-Bay K8644 in nanodiscs at 3.0 Angstrom resolution Deposited 2020-08-09	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain A 1–1873（1873 aa）	Not recorded	CA CALCIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 C8U methyl (4~{S})-2,6-dimethyl-5-nitro-4-[2-(trifluoromethyl)phenyl]-1,4-dihydropyridine-3-carboxylate × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE；blot for 5 seconds before plunging	Resolution 3.00 Å
7JPL Rabbit Cav1.1 in the presence of 10 micromolar (S)-(-)-Bay K8644 in nanodiscs at 3.4 Angstrom resolution Deposited 2020-08-09	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain A 1–1873（1873 aa）	Not recorded	CA CALCIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 C8U methyl (4~{S})-2,6-dimethyl-5-nitro-4-[2-(trifluoromethyl)phenyl]-1,4-dihydropyridine-3-carboxylate × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE；blot for 5 seconds before plunging	Resolution 3.40 Å
7JPV Rabbit Cav1.1 in the presence of 1 micromolar (S)-(-)-Bay K8644 in nanodiscs at 3.4 Angstrom resolution Deposited 2020-08-10	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain A 1–1873（1873 aa）	Not recorded	CA CALCIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE；blot for 5 seconds before plunging	Resolution 3.40 Å
7JPW Rabbit Cav1.1 in the presence of 100 micromolar (R)-(+)-Bay K8644 in nanodiscs at 3.2 Angstrom resolution Deposited 2020-08-10	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain A 1–1873（1873 aa）	Not recorded	CA CALCIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 VFY methyl (4R)-2,6-dimethyl-5-nitro-4-[2-(trifluoromethyl)phenyl]-1,4-dihydropyridine-3-carboxylate × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE；blot for 5 seconds before plunging	Resolution 3.20 Å
7JPX Rabbit Cav1.1 in the presence of 100 micromolar amlodipine in nanodiscs at 2.9 Angstrom resolution Deposited 2020-08-10	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 3 PDB declaration: trimeric	Chain A 1–1873（1873 aa）	Not recorded	CA CALCIUM ION × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 9 PC1 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE × 1 POV (2S)-3-(hexadecanoyloxy)-2-[(9Z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate × 1 6UB amlodipine × 1	ELECTRON MICROSCOPY cryo-EM buffer pH 8 cryo-EM vitrification conditions Cryogen ETHANE；blot for 5 seconds before plunging	Resolution 2.90 Å
7RXQ Crystal structure of junctophilin-2 in complex with a CaV1.1 peptide Deposited 2021-08-23	Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 1594–1609（16 aa） Fragment：EERIFRRTGGLFGQVD correspond to UNP residues 1594-1609	Not recorded	EOH ETHANOL × 1 SO4 SULFATE ION × 3	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；298 K；PEG3350, potassium sulphate	Resolution 2.03 Å R-free 0.252
8E56 Rabbit L-type voltage-gated calcium channel Cav1.1 in the presence of Amiodarone at 2.8 Angstrom resolution Deposited 2022-08-20	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric	Chain A 1–1873（1873 aa）	Not recorded	CA CALCIUM ION × 2 BBI (2-butyl-1-benzofuran-3-yl){4-[2-(diethylamino)ethoxy]-3,5-diiodophenyl}methanone × 1 3PE 1,2-Distearoyl-sn-glycerophosphoethanolamine × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 7	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.80 Å
8E57 Rabbit L-type voltage-gated calcium channel Cav1.1 in the presence of Amiodarone and 100 microM MNI-1 at 2.8 Angstrom resolution Deposited 2022-08-20	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric	Chain A 1–1873（1873 aa）	Not recorded	CA CALCIUM ION × 2 BBI (2-butyl-1-benzofuran-3-yl){4-[2-(diethylamino)ethoxy]-3,5-diiodophenyl}methanone × 1 WFR propan-2-yl (2S)-2-{[(S)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-4-ethynyl-3-hydroxy-4-methyloxolan-2-yl]methoxy}(phenoxy)phosphoryl]amino}propanoate (non-preferred name) × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 7	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 2.80 Å
8E58 Rabbit L-type voltage-gated calcium channel Cav1.1 in the presence of Amiodarone and 1 mM MNI-1 at 3.0 Angstrom resolution Deposited 2022-08-20	Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Other combination Heteromer；Protein × 3 PDB declaration: trimeric	Chain A 1–1873（1873 aa）	Not recorded	CA CALCIUM ION × 2 BBI (2-butyl-1-benzofuran-3-yl){4-[2-(diethylamino)ethoxy]-3,5-diiodophenyl}methanone × 1 WFR propan-2-yl (2S)-2-{[(S)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-4-ethynyl-3-hydroxy-4-methyloxolan-2-yl]methoxy}(phenoxy)phosphoryl]amino}propanoate (non-preferred name) × 1 NAG 2-acetamido-2-deoxy-beta-D-glucopyranose × 7	ELECTRON MICROSCOPY cryo-EM buffer pH 7.4 cryo-EM vitrification conditions Cryogen ETHANE	Resolution 3.00 Å

18 other PDB entries and 18 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	CAC1S_RABIT
Isoform	—
PDB entities	2
Chains and sequence ranges	Author chain B； PDBConstruct 1–18； UniProt 357–374

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	1t3l
Deposition date deposition_date	2004-04-27
Structure title title	Structural Analysis of the Voltage-Dependent Calcium Channel Beta Subunit Functional Core in Complex with Alpha1 Interaction Domain
Keywords keywords	protein-peptide complex, SH3 domain, Guanylate Kinase domain, transport protein; TRANSPORT PROTEIN
Experimental Method method	X-RAY DIFFRACTION

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	24.74 Å
Radius of gyration Rg (electron density) rg_electron	23.92 Å
Forward intensity I(0) i0	22253300.00
Molecular weight molecular_weight	36108.0 kDa
Excluded volume excluded_volume	45332 Å³
Envelope volume envelope_volume	56843 Å³
Hydration-shell volume shell_volume	21016 Å³
Envelope diameter envelope_diameter	94.4 Å
Shell Rg shell_rg	29.43 Å
Envelope Rg envelope_rg	24.75 Å
Shape Rg shape_rg	23.89 Å
Total Rg total_rg	24.70 Å
Total atoms total_atoms	2543
Residues n_residues	323
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	95.6 Å
Rg (real space) rg_real	24.93 Å
Rg uncertainty (real space) rg_real_error	1.06 Å
I(0) (real space) i0_real	2.2250e+07
I(0) uncertainty (real space) i0_real_error	3.5080e+05
Rg (reciprocal space) rg_reciprocal	24.89 Å
I(0) (reciprocal space) i0_reciprocal	22250000.0000
Solution quality estimate total_estimate	0.7079
Solution quality rating solution_quality	REASONABLE a REASONABLE solution
P(r) peaks n_peaks	2
Primary peak position r_peak_primary	24.3 Å
Skewness Skewness skewness	0.519
Kurtosis Kurtosis kurtosis	-0.125
Angular range angular_range	— – 0.3200 Å⁻¹
Current regularization parameter α current_alpha	0.0000
Highest regularization parameter α highest_alpha	4567000.0000
Real-space data points n_real_points	64
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.000; Oscil: 0.572; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.484; Smooth: 0.000

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Description description	Dihydropyridine-sensitive L-type, calcium channel beta-2 subunit
Molecular weight formula_weight	38341.0 kDa
Mutation mutation	P122R
Source method src_method	man
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	2671

SRPSDSDVSLEEDREAVRREAERQAQAQLEKAKTKPVAFAVRTNVSYSAAHEDDVPVPGMAISFEAKDFLHVKEKFNNDWWIGRLVKEGCEIGFIPSRVKLENMRLQHEQRAKEFKLHSKEKRMPFFKKTEHTPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFEGRISITRVTADISLAKRSVLNNPSKHAIIERSNTRSSLAEVQSEIERIFELARTLQLVVLDADTINHPAQLSKTSLAPIVVYVKISSPKVLQRLIKSRGKSQAKHLNVQMVAADKLAQCPPELFDVILDENQLEDACEHLADYLEAYWKATHPPSSNLPNPLL

来源 Source

来源生物 organism	Oryctolagus cuniculus
通用名 common_name	rabbit
NCBI Class编号 ncbi_taxonomy_id	9986
宿主生物 host_organism	Escherichia coli BL21(DE3)

Entity 2 Entity 2 polymer

Description description	Voltage-dependent L-type calcium channel alpha-1S subunit
Molecular weight formula_weight	2184.4 kDa
Source method src_method	syn
Copy count entity_count	1

Polymer Polymer

Polymer type type	polypeptide(L)
Chain IDs strand_id	B

QQLEEDLRGYMSWITQGE

Entity 3 Entity 3 water

Description description	water
Molecular weight formula_weight	18.0 kDa
Source method src_method	nat
Copy count entity_count	128

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7. Fold Classification (SCOP + CATH) 5 domains

SCOP 2.08 (3 domains)

Domain ID domain_id	d1t3la1
Class class	b — All beta proteins
Fold Fold fold	b.34 — SH3-like barrel
Superfamily Superfamily superfamily	b.34.2 — SH3-domain
Family Family family	b.34.2.1 — SH3-domain

Domain ID domain_id	d1t3la2
Class class	c — Alpha and beta proteins (a/b)
Fold Fold fold	c.37 — P-loop containing nucleoside triphosphate hydrolases
Superfamily Superfamily superfamily	c.37.1 — P-loop containing nucleoside triphosphate hydrolases
Family Family family	c.37.1.1 — Nucleotide and nucleoside kinases

Domain ID domain_id	d1t3lb_
Class class	j — Peptides
Fold Fold fold	j.68 — Fragments of the L-type calcium channel alpha subunit Cav.1 (dihydropyridine receptor)
Superfamily Superfamily superfamily	j.68.1 — Fragments of the L-type calcium channel alpha subunit Cav.1 (dihydropyridine receptor)
Family Family family	j.68.1.1 — Fragments of the L-type calcium channel alpha subunit Cav.1 (dihydropyridine receptor)

CATH v4.4 (2 domains)

Domain ID domain_id	1t3lA01
Class class	2 — Mainly Beta
Architecture architecture	30 — Roll
Topology topology	30 — SH3 type barrels.
Homologous superfamily homologous superfamily	40 — SH3 Domains

Domain ID domain_id	1t3lA02
Class class	3 — Alpha Beta
Architecture architecture	40 — 3-Layer(aba) Sandwich
Topology topology	50 — Rossmann fold
Homologous superfamily homologous superfamily	300 — P-loop containing nucleotide triphosphate hydrolases

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8. Citations (2)

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Unit-cell length a length_a	72.775 Å
Unit-cell length b length_b	168.262 Å
Unit-cell length c length_c	34.233 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	4

Space group space_group	P 21 21 2
Space-group number int_tables_number	18
Crystal system crystal_system	—

Resolution resolution	2.200 Å
Low-resolution limit resolution_low	84.520 Å
R factor r_factor	0.2255
R_work r_work	0.2214
R_free r_free	0.2820

fit	/app/data/saxs_computation/t3/1t3l/1t3l.fit
pddf	/app/data/pr_computation/t3/1t3l/1t3l.pr.out
profile	/app/data/saxs_computation/t3/1t3l/1t3l.dat

Structural Analysis of the Voltage-Dependent Calcium Channel Beta Subunit Functional Core in Complex with Alpha1 Interaction Domain

1. Protein Identity and Related Structures Protein Identity & Related Structures

Dihydropyridine-sensitive L-type, calcium channel beta-2 subunit

State in the Current Structure

Other States of the Same Protein in the Database

Voltage-dependent L-type calcium channel alpha-1S subunit

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

Refinement Refinement

Diffraction experiment Diffraction

6. Entities and Polymers Entities & Polymers (3)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

Entity 2 Entity 2 polymer

Polymer Polymer

Entity 3 Entity 3 water

7. Fold Classification (SCOP + CATH) 5 domains

SCOP 2.08 (3 domains)

CATH v4.4 (2 domains)

8. Citations (2)

9. Files and Curves (10)

Files (7)

Curves (3)