1uw9

L290F-A222T chlamydomonas Rubisco mutant

Method: X-RAY DIFFRACTION Dmax: 135.2 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1uw9

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1uw9
下载 Download

1. 结构基本信息 1. Structure Basics

条目编号 entry_id1uw9
沉积日期 deposition_date2004-02-03
结构标题 titleL290F-A222T chlamydomonas Rubisco mutant
关键词 keywordsLYASE, RUBISCO, PHOTOSYNTHESIS, PHOTORESPIRATION, OXIDOREDUCTASE, MONOOXYGENASE, CARBON DIOXIDE FIXATION; LYASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier47.04
回转半径 Rg (电子) rg_electron46.22
零角强度 I(0) i04371220000.00
分子量 molecular_weight551160.0 kDa
排除体积 excluded_volume687120 ų
包络体积 envelope_volume813890 ų
水化壳体积 shell_volume131320 ų
包络直径 envelope_diameter136.6
壳层 Rg shell_rg60.14
包络 Rg envelope_rg46.17
形状 Rg shape_rg46.22
总 Rg total_rg46.57
总原子数 total_atoms38700
残基数 n_residues4815
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax135.2
Rg (实空间) rg_real46.57
Rg 误差 (实空间) rg_real_error0.46
I(0) (实空间) i0_real4.3710e+09
I(0) 误差 (实空间) i0_real_error5.6410e+07
Rg (倒空间) rg_reciprocal47.04
I(0) (倒空间) i0_reciprocal4374000000.0000
解质量估计 total_estimate0.8761
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary63.6
偏度 Skewness skewness-0.027
峰度 Kurtosis kurtosis-0.582
角度范围 angular_range— – 0.1700 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha4065000000.0000
实空间数据点数 n_real_points35
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.944; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.967; Smooth: 0.585

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (6)

6. 折叠分类 (SCOP + CATH) 48 domains

SCOP 2.08 (24 domains)

结构域编号 domain_idd1uw9a1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1uw9a2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.0 — automated matches
结构域编号 domain_idd1uw9b1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1uw9b2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.0 — automated matches
结构域编号 domain_idd1uw9c_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1uw9e1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1uw9e2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.0 — automated matches
结构域编号 domain_idd1uw9f_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1uw9h1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1uw9h2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.0 — automated matches
结构域编号 domain_idd1uw9i_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1uw9j_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1uw9k1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1uw9k2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.0 — automated matches
结构域编号 domain_idd1uw9m_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1uw9o1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1uw9o2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.0 — automated matches
结构域编号 domain_idd1uw9p_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1uw9r1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1uw9r2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.0 — automated matches
结构域编号 domain_idd1uw9t_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1uw9v1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1uw9v2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.0 — automated matches
结构域编号 domain_idd1uw9w_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit

CATH v4.4 (24 domains)

结构域编号 domain_id1uw9A01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1uw9A02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1uw9B01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1uw9B02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1uw9C00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1uw9E01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1uw9E02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1uw9F00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1uw9H01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1uw9H02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1uw9I00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1uw9J00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1uw9K01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1uw9K02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1uw9M00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1uw9O01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1uw9O02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1uw9P00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1uw9R01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1uw9R02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1uw9T00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1uw9V01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1uw9V02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1uw9W00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit

7. 引用文献 (1)

8. 文件与曲线 (10)