1uwa

L290F mutant rubisco from chlamydomonas

Method: X-RAY DIFFRACTION Dmax: 136.8 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1uwa

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1uwa
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1uwa
沉积日期 deposition_date2004-02-03
结构标题 titleL290F mutant rubisco from chlamydomonas
关键词 keywordsLYASE, RUBISCO, PHOTOSYNTHESIS, PHOTORESPIRATION, OXIDOREDUCTASE, MONOOXYGENASE, CARBON DIOXIDE FIXATION; LYASE
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier47.18
回转半径 Rg (电子) rg_electron46.35
零角强度 I(0) i04359560000.00
分子量 molecular_weight550330.0 kDa
排除体积 excluded_volume685990 ų
包络体积 envelope_volume816370 ų
水化壳体积 shell_volume131560 ų
包络直径 envelope_diameter139.1
壳层 Rg shell_rg60.18
包络 Rg envelope_rg46.23
形状 Rg shape_rg46.36
总 Rg total_rg46.70
总原子数 total_atoms38644
残基数 n_residues4816
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax136.8
Rg (实空间) rg_real46.70
Rg 误差 (实空间) rg_real_error0.54
I(0) (实空间) i0_real4.3600e+09
I(0) 误差 (实空间) i0_real_error6.1150e+07
Rg (倒空间) rg_reciprocal47.18
I(0) (倒空间) i0_reciprocal4362000000.0000
解质量估计 total_estimate0.8786
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary66.3
偏度 Skewness skewness-0.029
峰度 Kurtosis kurtosis-0.583
角度范围 angular_range— – 0.1650 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha3646000000.0000
实空间数据点数 n_real_points34
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.936; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.947; Smooth: 0.662

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (6)

6. 折叠分类 (SCOP + CATH) 48 domains

SCOP 2.08 (24 domains)

结构域编号 domain_idd1uwaa1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1uwaa2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.0 — automated matches
结构域编号 domain_idd1uwab1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1uwab2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.0 — automated matches
结构域编号 domain_idd1uwac_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1uwae1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1uwae2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.0 — automated matches
结构域编号 domain_idd1uwaf_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1uwah1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1uwah2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.0 — automated matches
结构域编号 domain_idd1uwai_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1uwaj_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1uwak1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1uwak2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.0 — automated matches
结构域编号 domain_idd1uwam_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1uwao1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1uwao2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.0 — automated matches
结构域编号 domain_idd1uwap_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1uwar1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1uwar2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.0 — automated matches
结构域编号 domain_idd1uwat_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit
结构域编号 domain_idd1uwav1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.1 — TIM beta/alpha-barrel
超家族 Superfamily superfamilyc.1.14 — RuBisCo, C-terminal domain
家族 Family familyc.1.14.1 — RuBisCo, large subunit, C-terminal domain
结构域编号 domain_idd1uwav2
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.58 — Ferredoxin-like
超家族 Superfamily superfamilyd.58.9 — RuBisCO, large subunit, small (N-terminal) domain
家族 Family familyd.58.9.0 — automated matches
结构域编号 domain_idd1uwaw_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.73 — RuBisCO, small subunit
超家族 Superfamily superfamilyd.73.1 — RuBisCO, small subunit
家族 Family familyd.73.1.1 — RuBisCO, small subunit

CATH v4.4 (24 domains)

结构域编号 domain_id1uwaA01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1uwaA02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1uwaB01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1uwaB02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1uwaC00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1uwaE01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1uwaE02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1uwaF00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1uwaH01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1uwaH02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1uwaI00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1uwaJ00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1uwaK01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1uwaK02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1uwaM00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1uwaO01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1uwaO02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1uwaP00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1uwaR01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1uwaR02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1uwaT00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit
结构域编号 domain_id1uwaV01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology70 — Alpha-Beta Plaits
同源超家族 H-superfamily homologous superfamily150 — RuBisCO large subunit, N-terminal domain
结构域编号 domain_id1uwaV02
类 Class class3 — Alpha Beta
架构 Architecture architecture20 — Alpha-Beta Barrel
拓扑 Topology topology20 — TIM Barrel
同源超家族 H-superfamily homologous superfamily110 — Ribulose bisphosphate carboxylase, large subunit, C-terminal domain
结构域编号 domain_id1uwaW00
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology190 — Ribulose 1,5 Bisphosphate Carboxylase/Oxygenase
同源超家族 H-superfamily homologous superfamily10 — Ribulose bisphosphate carboxylase, small subunit

7. 引用文献 (1)

8. 文件与曲线 (10)