splicing factor, arginine/serine-rich 1 (splicing factor 2, alternate splicing factor) variant
Homo sapiens
State in the Current Structure
| Assembly | Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Associated Components | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|
| 1 | Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count | Chain A； UniProt 33–128 | Fragment：RRM domain | No other associated polymer | SOLUTION NMR NMR measurement conditions：pH 7；298 K；Ionic strength (raw mmCIF value) 120mM；Pressure ambient NMR sample composition：0.8mM U-15,13C; 20mM phosphate buffer NA; 100mM NaCl; 1mM d-DTT; 0.02% NaN3 | 90% H2O/10% D2O | Resolution not provided |
Other States of the Same Protein in the Database
Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.
| Other PDB | Difference from Current Entry 1X4A | Assembly / Oligomeric State | Construct | Mutations and Modifications | Ligands, Ions and Non-polymers | Method and Experimental Conditions | Structure Quality |
|---|---|---|---|---|---|---|---|
| 2M7S NMR structure of RNA recognition motif 2 (RRM2) of Homo sapiens splicing factor, arginine/serine-rich 1 Deposited 2013-04-29 | Different construct Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–195（90 aa）
Fragment：UNP residues 106-195
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6；298 K；Ionic strength (raw mmCIF value) 0.0798；Pressure ambient
NMR sample composition
1.2 mM [U-99% 13C; U-99% 15N] protein, 20 mM sodium phosphate, 50 mM sodium chloride, 5 mM sodium azide, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2M8D Structure of SRSF1 RRM2 in complex with the RNA 5'-UGAAGGAC-3' Deposited 2013-05-17 | Different construct Different oligomeric state Different experimental conditions | Assembly 1 Protein–RNA Monomer；Protein × 1 PDB declaration: dimeric |
Chain B
107–196（90 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 5.5；313 K；Ionic strength (raw mmCIF value) 120；Pressure ambient
NMR sample composition
0.8 mM [U-100% 15N] RNA, 0.8 mM [U-100% 13C; U-100% 15N] protein, 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 2O3D Structure of human SF2/ASF RNA recognition motif 2 (RRM2) Deposited 2006-12-01 | Different construct Different experimental conditions | Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric |
Chain A
106–214（109 aa）
Fragment：RNA Recognition motif 2 of SF2/ASF
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 6；303 K；Ionic strength (raw mmCIF value) 20mM phosphate, 50mM NaCl, 50mM L-Arg, 50mM L-Glu；Pressure ambient
NMR sample composition
1mM SF2/ASF RRM2 U-15N,13C; 20mM phophate buffer Na; 50mM L-Arg; 50mM L-Glu; 5mM EDTA; 1mM DTT; 90% H2O, 10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 3BEG Crystal structure of SR protein kinase 1 complexed to its substrate ASF/SF2 Deposited 2007-11-18 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain B
105–219（115 aa）
Fragment：UNP residues 105-219
|
Not recorded | SEP PHOSPHOSERINE × 1 ALA ALANINE × 1 ANP PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
VAPOR DIFFUSION, HANGING DROP；pH 5.6；291 K；0.1M sodium citrate, 0.2M sodium acetate, 5% ethanol, 15% PEGMME 5000, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K
|
Resolution 2.90 Å R-free 0.298 |
| 4C0O Transportin 3 in complex with phosphorylated ASF/SF2 Deposited 2013-08-06 | Different construct Different mutation/modification Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain C
106–230（125 aa）
Fragment：RESIDUES 106-230 (SECOND RRM DOMAIN AND RS DOMAIN)
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | No recorded non-water small molecule |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6；16% (W/V) PEG3350, 0.1 M KSCN, 0.1 M BIS-TRIS PROPANE, PH6.0
|
Resolution 2.56 Å R-free 0.269 |
| 4C0O Transportin 3 in complex with phosphorylated ASF/SF2 Deposited 2013-08-06 | Different construct Different mutation/modification Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric |
Chain D
106–230（125 aa）
Fragment：RESIDUES 106-230 (SECOND RRM DOMAIN AND RS DOMAIN)
|
Non-standard monomer：Yes (specific site not provided by mmCIF) | K POTASSIUM ION × 1 |
X-RAY DIFFRACTION
X-ray crystallization conditions
pH 6；16% (W/V) PEG3350, 0.1 M KSCN, 0.1 M BIS-TRIS PROPANE, PH6.0
|
Resolution 2.56 Å R-free 0.269 |
| 6HPJ Structure of human SRSF1 RRM1 bound to AACAAA RNA Deposited 2018-09-21 | Different construct Different oligomeric state Different experimental conditions | Assembly 1 Insufficient information Monomer；Protein × 1 PDB declaration: dimeric |
Chain B
1–97（97 aa）
|
Not recorded | No recorded non-water small molecule |
SOLUTION NMR
NMR measurement conditions
pH 7；313 K；Ionic strength (raw mmCIF value) 120；Pressure atmospheric
NMR sample composition
0.5 mM [U-99% 15N] SRSF1 RRM1, 0.5 mM RNA (5'-R(*AP*AP*CP*AP*AP*A)-3'), 100% D2O | 100% D2O
NMR sample composition
0.5 mM [U-99% 15N] SRSF1 RRM1, 0.5 mM NA RNA (5'-R(*AP*AP*CP*AP*AP*A)-3'), 90% H2O/10% D2O | 90% H2O/10% D2O
NMR sample composition
0.5 mM [U-99% 13C; U-99% 15N] SRSF1 RRM1, 0.5 mM NA RNA (5'-R(*AP*AP*CP*AP*AP*A)-3'), 90% H2O/10% D2O | 90% H2O/10% D2O
|
Resolution not provided |
| 7ABG Human pre-Bact-1 spliceosome Deposited 2020-09-07 | Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 54 PDB declaration: 58-meric |
Chain A6
1–248（248 aa）
|
Not recorded | IHP INOSITOL HEXAKISPHOSPHATE × 1 GTP GUANOSINE-5'-TRIPHOSPHATE × 1 MG MAGNESIUM ION × 1 GTG 7-METHYL-GUANOSINE-5'-TRIPHOSPHATE-5'-GUANOSINE × 1 |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 7.80 Å |
| 8QO9 Cryo-EM structure of a human spliceosomal B complex protomer Deposited 2023-09-28 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 66 PDB declaration: 71-meric |
Chain z
1–248（248 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 5.29 Å |
| 9R8V Cryo-EM structure of the human pre-Bact-OTS complex (whole map) Deposited 2025-05-17 | Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics | Assembly 1 Protein–RNA Heteromer；Protein × 59 PDB declaration: 63-meric |
Chain A6
1–248（248 aa）
|
Not recorded | No recorded non-water small molecule |
ELECTRON MICROSCOPY
cryo-EM buffer
pH 7.9
cryo-EM vitrification conditions
Cryogen ETHANE
|
Resolution 8.50 Å |
9 other PDB entries and 10 assemblies. Open the comparison page and filter oligomeric states
View Construct and Data Evidence
| UniProt name | SFRS1_HUMAN |
| Isoform | — |
| PDB entities | 1 |
| Chains and sequence ranges | Author chain A； PDBConstruct 8–103； UniProt 33–128 |
