← Back to search
1xfu

Crystal structure of anthrax edema factor (EF) truncation mutant, EF-delta 64 in complex with calmodulin

Method: X-RAY DIFFRACTION
▼

1. Protein Identity and Related Structures Protein Identity & Related Structures

Calmodulin-sensitive adenylate cyclase

Bacillus anthracis

UniProt P40136

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 2 Calmodulin 2 × 1 （P62158） MAGNESIUM ION × 1 CALCIUM ION × 3 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 2 Calmodulin 2 × 1 （P62158） MAGNESIUM ION × 1 CALCIUM ION × 3 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 2 Calmodulin 2 × 1 （P62158） MAGNESIUM ION × 1 CALCIUM ION × 3 Consistent with protein count
4 Protein heterocomplex Heteromer Protein 2 Calmodulin 2 × 1 （P62158） MAGNESIUM ION × 1 CALCIUM ION × 3 Consistent with protein count
5 Protein heterocomplex Heteromer Protein 2 Calmodulin 2 × 1 （P62158） MAGNESIUM ION × 1 CALCIUM ION × 3 Consistent with protein count
6 Protein heterocomplex Heteromer Protein 2 Calmodulin 2 × 1 （P62158） MAGNESIUM ION × 1 CALCIUM ION × 3 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1k8t

1 assemblies

Homodimer
1k8t

1 assemblies

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1k90 1k90 1k90 1k90 1k93 1k93 1k93 1lvc 1lvc 1lvc 1pk0 1pk0

69 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name CYAA_BACAN
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 11–747； UniProt 64–800 Author chain B； PDBConstruct 11–747； UniProt 64–800 Author chain C； PDBConstruct 11–747； UniProt 64–800 Author chain D； PDBConstruct 11–747； UniProt 64–800 Author chain E； PDBConstruct 11–747； UniProt 64–800 Author chain F； PDBConstruct 11–747； UniProt 64–800

Calmodulin 2

Homo sapiens

UniProt P62158

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Protein heterocomplex Heteromer Protein 2 Calmodulin-sensitive adenylate cyclase × 1 （P40136） MAGNESIUM ION × 1 CALCIUM ION × 3 Consistent with protein count
2 Protein heterocomplex Heteromer Protein 2 Calmodulin-sensitive adenylate cyclase × 1 （P40136） MAGNESIUM ION × 1 CALCIUM ION × 3 Consistent with protein count
3 Protein heterocomplex Heteromer Protein 2 Calmodulin-sensitive adenylate cyclase × 1 （P40136） MAGNESIUM ION × 1 CALCIUM ION × 3 Consistent with protein count
4 Protein heterocomplex Heteromer Protein 2 Calmodulin-sensitive adenylate cyclase × 1 （P40136） MAGNESIUM ION × 1 CALCIUM ION × 3 Consistent with protein count
5 Protein heterocomplex Heteromer Protein 2 Calmodulin-sensitive adenylate cyclase × 1 （P40136） MAGNESIUM ION × 1 CALCIUM ION × 3 Consistent with protein count
6 Protein heterocomplex Heteromer Protein 2 Calmodulin-sensitive adenylate cyclase × 1 （P40136） MAGNESIUM ION × 1 CALCIUM ION × 3 Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1cll 1ctr 1j7o 1j7p 1sw8 1y6w 2hf5 2k0e 2k0j 2k61 2kuh 2lqp

22 assemblies; showing the first 12

Homodimer
3uct 3uct 3ucw 3ucw 3ucy 4bw7

6 assemblies

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1cdl 1cdl 1cdl 1cdl 1iwq 1iwq 1l7z 1pk0 1pk0 1pk0 1pk0 1s26

149 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name CALM_HUMAN
Isoform —
PDB entities 2
Chains and sequence ranges Author chain O； PDBConstruct 1–149； UniProt 1–149 Author chain P； PDBConstruct 1–149； UniProt 1–149 Author chain Q； PDBConstruct 1–149； UniProt 1–149 Author chain R； PDBConstruct 1–149； UniProt 1–149 Author chain S； PDBConstruct 1–149； UniProt 1–149 Author chain T； PDBConstruct 1–149； UniProt 1–149

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

▼

2. Structure Basics 2. Structure Basics

Entry ID entry_id1xfu
Deposition date deposition_date2004-09-15
Structure title titleCrystal structure of anthrax edema factor (EF) truncation mutant, EF-delta 64 in complex with calmodulin
Keywords keywordsprotein-protein complex, LYASE-Metal binding protein COMPLEX; LYASE/Metal binding protein
Experimental Method methodX-RAY DIFFRACTION
▼

3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

1xfu__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

1xfu__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

1xfu__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）37.96 Å
Rg (electron density)37.63 Å
Total Rg37.91 Å
Atom count7142
Residues881
Excluded volume127370 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 1xfu__assembly_1__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
2 1 1xfu__assembly_2__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
3 1 1xfu__assembly_3__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
4 1 1xfu__assembly_4__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
5 1 1xfu__assembly_5__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
6 1 1xfu__assembly_6__model_1 dimeric （2） Success 4.1.3-1-20251215 (887e7ef) View Download
▶

4. Crystallography and Experiment 4. Crystallography & Experiment

▶

5. Entities and Polymers Entities & Polymers (4)

▼

6. Fold Classification (SCOP + CATH) 36 domains

SCOP 2.08 (6 domains)

Domain ID domain_idd1xfuo1
Class classa — All alpha proteins
Fold Fold folda.39 — EF Hand-like
Superfamily Superfamily superfamilya.39.1 — EF-hand
Family Family familya.39.1.5 — Calmodulin-like
Domain ID domain_idd1xfup1
Class classa — All alpha proteins
Fold Fold folda.39 — EF Hand-like
Superfamily Superfamily superfamilya.39.1 — EF-hand
Family Family familya.39.1.5 — Calmodulin-like
Domain ID domain_idd1xfuq1
Class classa — All alpha proteins
Fold Fold folda.39 — EF Hand-like
Superfamily Superfamily superfamilya.39.1 — EF-hand
Family Family familya.39.1.5 — Calmodulin-like
Domain ID domain_idd1xfur1
Class classa — All alpha proteins
Fold Fold folda.39 — EF Hand-like
Superfamily Superfamily superfamilya.39.1 — EF-hand
Family Family familya.39.1.5 — Calmodulin-like
Domain ID domain_idd1xfus1
Class classa — All alpha proteins
Fold Fold folda.39 — EF Hand-like
Superfamily Superfamily superfamilya.39.1 — EF-hand
Family Family familya.39.1.5 — Calmodulin-like
Domain ID domain_idd1xfut1
Class classa — All alpha proteins
Fold Fold folda.39 — EF Hand-like
Superfamily Superfamily superfamilya.39.1 — EF-hand
Family Family familya.39.1.5 — Calmodulin-like

CATH v4.4 (30 domains)

Domain ID domain_id1xfuA01
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology390 — Collagenase (Catalytic Domain)
Homologous superfamily homologous superfamily10 — Collagenase (Catalytic Domain)
Domain ID domain_id1xfuA03
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology1760 — Adenylylcyclase toxin fold
Homologous superfamily homologous superfamily10 — Anthrax toxin, edema factor, central domain
Domain ID domain_id1xfuA04
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology140 — Butyryl-CoA Dehydrogenase, subunit A; domain 3
Homologous superfamily homologous superfamily60 —
Domain ID domain_id1xfuB01
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology390 — Collagenase (Catalytic Domain)
Homologous superfamily homologous superfamily10 — Collagenase (Catalytic Domain)
Domain ID domain_id1xfuB03
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology1760 — Adenylylcyclase toxin fold
Homologous superfamily homologous superfamily10 — Anthrax toxin, edema factor, central domain
Domain ID domain_id1xfuB04
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology140 — Butyryl-CoA Dehydrogenase, subunit A; domain 3
Homologous superfamily homologous superfamily60 —
Domain ID domain_id1xfuC01
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology390 — Collagenase (Catalytic Domain)
Homologous superfamily homologous superfamily10 — Collagenase (Catalytic Domain)
Domain ID domain_id1xfuC03
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology1760 — Adenylylcyclase toxin fold
Homologous superfamily homologous superfamily10 — Anthrax toxin, edema factor, central domain
Domain ID domain_id1xfuC04
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology140 — Butyryl-CoA Dehydrogenase, subunit A; domain 3
Homologous superfamily homologous superfamily60 —
Domain ID domain_id1xfuD01
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology390 — Collagenase (Catalytic Domain)
Homologous superfamily homologous superfamily10 — Collagenase (Catalytic Domain)
Domain ID domain_id1xfuD03
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology1760 — Adenylylcyclase toxin fold
Homologous superfamily homologous superfamily10 — Anthrax toxin, edema factor, central domain
Domain ID domain_id1xfuD04
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology140 — Butyryl-CoA Dehydrogenase, subunit A; domain 3
Homologous superfamily homologous superfamily60 —
Domain ID domain_id1xfuE01
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology390 — Collagenase (Catalytic Domain)
Homologous superfamily homologous superfamily10 — Collagenase (Catalytic Domain)
Domain ID domain_id1xfuE03
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology1760 — Adenylylcyclase toxin fold
Homologous superfamily homologous superfamily10 — Anthrax toxin, edema factor, central domain
Domain ID domain_id1xfuE04
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology140 — Butyryl-CoA Dehydrogenase, subunit A; domain 3
Homologous superfamily homologous superfamily60 —
Domain ID domain_id1xfuF01
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology390 — Collagenase (Catalytic Domain)
Homologous superfamily homologous superfamily10 — Collagenase (Catalytic Domain)
Domain ID domain_id1xfuF03
Class class3 — Alpha Beta
Architecture architecture90 — Alpha-Beta Complex
Topology topology1760 — Adenylylcyclase toxin fold
Homologous superfamily homologous superfamily10 — Anthrax toxin, edema factor, central domain
Domain ID domain_id1xfuF04
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology140 — Butyryl-CoA Dehydrogenase, subunit A; domain 3
Homologous superfamily homologous superfamily60 —
Domain ID domain_id1xfuO01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1xfuO02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1xfuP01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1xfuP02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1xfuQ01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1xfuQ02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1xfuR01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1xfuR02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1xfuS01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1xfuS02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1xfuT01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
Domain ID domain_id1xfuT02
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology238 — Recoverin; domain 1
Homologous superfamily homologous superfamily10 — EF-hand
▶

7. Citations (1)