1yyl

;crystal structure of CD4M33, a scorpion-toxin mimic of CD4, in complex with HIV-1 YU2 gp120 envelope glycoprotein and anti-HIV-1 antibody 17b ;

Method: X-RAY DIFFRACTION Dmax: 154.6 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 1yyl

P(r) 距离分布 P(r) Distribution

P(r) distribution for 1yyl
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id1yyl
沉积日期 deposition_date2005-02-25
结构标题 title;crystal structure of CD4M33, a scorpion-toxin mimic of CD4, in complex with HIV-1 YU2 gp120 envelope glycoprotein and anti-HIV-1 antibody 17b ;
关键词 keywordsHIV-1, gp120, YU2, scorpion toxin, CD4 mimic, CD4M33, antibody, Viral protein-Immune system COMPLEX; Viral protein/Immune system
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier45.00
回转半径 Rg (电子) rg_electron44.76
零角强度 I(0) i0454915000.00
分子量 molecular_weight171670.0 kDa
排除体积 excluded_volume213630 ų
包络体积 envelope_volume310980 ų
水化壳体积 shell_volume59617 ų
包络直径 envelope_diameter175.9
壳层 Rg shell_rg47.37
包络 Rg envelope_rg44.13
形状 Rg shape_rg44.72
总 Rg total_rg45.02
总原子数 total_atoms12046
残基数 n_residues1502
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax154.6
Rg (实空间) rg_real44.89
Rg 误差 (实空间) rg_real_error1.82
I(0) (实空间) i0_real4.5490e+08
I(0) 误差 (实空间) i0_real_error8.7630e+06
Rg (倒空间) rg_reciprocal45.00
I(0) (倒空间) i0_reciprocal455000000.0000
解质量估计 total_estimate0.8090
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks2
主峰位置 r_peak_primary61.9
偏度 Skewness skewness0.127
峰度 Kurtosis kurtosis-0.589
角度范围 angular_range— – 0.1750 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha37910000.0000
实空间数据点数 n_real_points36
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.848; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.970; Smooth: 0.000

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (6)

6. 折叠分类 (SCOP + CATH) 22 domains

SCOP 2.08 (12 domains)

结构域编号 domain_idd1yylg_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.172 — gp120 core
超家族 Superfamily superfamilyd.172.1 — gp120 core
家族 Family familyd.172.1.1 — gp120 core
结构域编号 domain_idd1yylh1
类 Class classb — All beta proteins
折叠类型 Fold foldb.1 — Immunoglobulin-like beta-sandwich
超家族 Superfamily superfamilyb.1.1 — Immunoglobulin
家族 Family familyb.1.1.1 — V set domains (antibody variable domain-like)
结构域编号 domain_idd1yylh2
类 Class classb — All beta proteins
折叠类型 Fold foldb.1 — Immunoglobulin-like beta-sandwich
超家族 Superfamily superfamilyb.1.1 — Immunoglobulin
家族 Family familyb.1.1.2 — C1 set domains (antibody constant domain-like)
结构域编号 domain_idd1yyll1
类 Class classb — All beta proteins
折叠类型 Fold foldb.1 — Immunoglobulin-like beta-sandwich
超家族 Superfamily superfamilyb.1.1 — Immunoglobulin
家族 Family familyb.1.1.1 — V set domains (antibody variable domain-like)
结构域编号 domain_idd1yyll2
类 Class classb — All beta proteins
折叠类型 Fold foldb.1 — Immunoglobulin-like beta-sandwich
超家族 Superfamily superfamilyb.1.1 — Immunoglobulin
家族 Family familyb.1.1.2 — C1 set domains (antibody constant domain-like)
结构域编号 domain_idd1yylm1
类 Class classk — Designed proteins
折叠类型 Fold foldk.23 — Scorpion toxin-based designs
超家族 Superfamily superfamilyk.23.1 — Scorpion toxin-based designs
家族 Family familyk.23.1.2 — Mini-protein reproducing the core of the CD4 surface
结构域编号 domain_idd1yylp_
类 Class classd — Alpha and beta proteins (a+b)
折叠类型 Fold foldd.172 — gp120 core
超家族 Superfamily superfamilyd.172.1 — gp120 core
家族 Family familyd.172.1.1 — gp120 core
结构域编号 domain_idd1yylq1
类 Class classb — All beta proteins
折叠类型 Fold foldb.1 — Immunoglobulin-like beta-sandwich
超家族 Superfamily superfamilyb.1.1 — Immunoglobulin
家族 Family familyb.1.1.1 — V set domains (antibody variable domain-like)
结构域编号 domain_idd1yylq2
类 Class classb — All beta proteins
折叠类型 Fold foldb.1 — Immunoglobulin-like beta-sandwich
超家族 Superfamily superfamilyb.1.1 — Immunoglobulin
家族 Family familyb.1.1.2 — C1 set domains (antibody constant domain-like)
结构域编号 domain_idd1yylr1
类 Class classb — All beta proteins
折叠类型 Fold foldb.1 — Immunoglobulin-like beta-sandwich
超家族 Superfamily superfamilyb.1.1 — Immunoglobulin
家族 Family familyb.1.1.1 — V set domains (antibody variable domain-like)
结构域编号 domain_idd1yylr2
类 Class classb — All beta proteins
折叠类型 Fold foldb.1 — Immunoglobulin-like beta-sandwich
超家族 Superfamily superfamilyb.1.1 — Immunoglobulin
家族 Family familyb.1.1.2 — C1 set domains (antibody constant domain-like)
结构域编号 domain_idd1yyls1
类 Class classk — Designed proteins
折叠类型 Fold foldk.23 — Scorpion toxin-based designs
超家族 Superfamily superfamilyk.23.1 — Scorpion toxin-based designs
家族 Family familyk.23.1.2 — Mini-protein reproducing the core of the CD4 surface

CATH v4.4 (10 domains)

结构域编号 domain_id1yylG00
类 Class class2 — Mainly Beta
架构 Architecture architecture170 — Beta Complex
拓扑 Topology topology40 — HIV Envelope Protein Gp120; Chain G
同源超家族 H-superfamily homologous superfamily20 — Human immunodeficiency virus 1, Gp160, envelope glycoprotein
结构域编号 domain_id1yylH01
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id1yylH02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id1yylL01
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id1yylL02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id1yylP00
类 Class class2 — Mainly Beta
架构 Architecture architecture170 — Beta Complex
拓扑 Topology topology40 — HIV Envelope Protein Gp120; Chain G
同源超家族 H-superfamily homologous superfamily20 — Human immunodeficiency virus 1, Gp160, envelope glycoprotein
结构域编号 domain_id1yylQ01
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id1yylQ02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id1yylR01
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id1yylR02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins

7. 引用文献 (1)

8. 文件与曲线 (10)