2ex3

Bacteriophage phi29 DNA polymerase bound to terminal protein

Method: X-RAY DIFFRACTION Dmax: 212.4 Å Quality: GOOD

SAXS 散射曲线 SAXS Profile

SAXS profile for 2ex3

P(r) 距离分布 P(r) Distribution

P(r) distribution for 2ex3
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1. 结构基本信息 1. Structure Basics

条目编号 entry_id2ex3
沉积日期 deposition_date2005-11-07
结构标题 titleBacteriophage phi29 DNA polymerase bound to terminal protein
关键词 keywordsDNA POLYMERASE: PROTEIN PRIMER COMPLEX, transferase-replication COMPLEX; transferase/replication
实验方法 methodX-RAY DIFFRACTION

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier72.29
回转半径 Rg (电子) rg_electron72.48
零角强度 I(0) i03694740000.00
分子量 molecular_weight528670.0 kDa
排除体积 excluded_volume665720 ų
包络体积 envelope_volume1092500 ų
水化壳体积 shell_volume128610 ų
包络直径 envelope_diameter253.2
壳层 Rg shell_rg67.83
包络 Rg envelope_rg70.13
形状 Rg shape_rg72.44
总 Rg total_rg72.53
总原子数 total_atoms37108
残基数 n_residues4596
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax212.4
Rg (实空间) rg_real72.19
Rg 误差 (实空间) rg_real_error0.94
I(0) (实空间) i0_real3.6820e+09
I(0) 误差 (实空间) i0_real_error6.5570e+07
Rg (倒空间) rg_reciprocal71.44
I(0) (倒空间) i0_reciprocal3687000000.0000
解质量估计 total_estimate0.8403
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks1
主峰位置 r_peak_primary77.2
偏度 Skewness skewness0.339
峰度 Kurtosis kurtosis-0.636
角度范围 angular_range— – 0.1100 −1
当前正则化参数 α current_alpha0.1267
最高正则化参数 α highest_alpha158900000.0000
实空间数据点数 n_real_points23
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.980; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.974; Smooth: 0.001

4. 晶体学与实验 4. Crystallography & Experiment

5. 实体与聚合体信息 (3)

6. 折叠分类 (SCOP + CATH) 66 domains

SCOP 2.08 (18 domains)

结构域编号 domain_idd2ex3a1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.55 — Ribonuclease H-like motif
超家族 Superfamily superfamilyc.55.3 — Ribonuclease H-like
家族 Family familyc.55.3.5 — DnaQ-like 3'-5' exonuclease
结构域编号 domain_idd2ex3a2
类 Class classe — Multi-domain proteins (alpha and beta)
折叠类型 Fold folde.8 — DNA/RNA polymerases
超家族 Superfamily superfamilye.8.1 — DNA/RNA polymerases
家族 Family familye.8.1.1 — DNA polymerase I
结构域编号 domain_idd2ex3b1
类 Class classa — All alpha proteins
折叠类型 Fold folda.263 — DNA terminal protein
超家族 Superfamily superfamilya.263.1 — DNA terminal protein
家族 Family familya.263.1.1 — DNA terminal protein
结构域编号 domain_idd2ex3c1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.55 — Ribonuclease H-like motif
超家族 Superfamily superfamilyc.55.3 — Ribonuclease H-like
家族 Family familyc.55.3.5 — DnaQ-like 3'-5' exonuclease
结构域编号 domain_idd2ex3c2
类 Class classe — Multi-domain proteins (alpha and beta)
折叠类型 Fold folde.8 — DNA/RNA polymerases
超家族 Superfamily superfamilye.8.1 — DNA/RNA polymerases
家族 Family familye.8.1.1 — DNA polymerase I
结构域编号 domain_idd2ex3d1
类 Class classa — All alpha proteins
折叠类型 Fold folda.263 — DNA terminal protein
超家族 Superfamily superfamilya.263.1 — DNA terminal protein
家族 Family familya.263.1.1 — DNA terminal protein
结构域编号 domain_idd2ex3e1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.55 — Ribonuclease H-like motif
超家族 Superfamily superfamilyc.55.3 — Ribonuclease H-like
家族 Family familyc.55.3.5 — DnaQ-like 3'-5' exonuclease
结构域编号 domain_idd2ex3e2
类 Class classe — Multi-domain proteins (alpha and beta)
折叠类型 Fold folde.8 — DNA/RNA polymerases
超家族 Superfamily superfamilye.8.1 — DNA/RNA polymerases
家族 Family familye.8.1.1 — DNA polymerase I
结构域编号 domain_idd2ex3f1
类 Class classa — All alpha proteins
折叠类型 Fold folda.263 — DNA terminal protein
超家族 Superfamily superfamilya.263.1 — DNA terminal protein
家族 Family familya.263.1.1 — DNA terminal protein
结构域编号 domain_idd2ex3g1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.55 — Ribonuclease H-like motif
超家族 Superfamily superfamilyc.55.3 — Ribonuclease H-like
家族 Family familyc.55.3.5 — DnaQ-like 3'-5' exonuclease
结构域编号 domain_idd2ex3g2
类 Class classe — Multi-domain proteins (alpha and beta)
折叠类型 Fold folde.8 — DNA/RNA polymerases
超家族 Superfamily superfamilye.8.1 — DNA/RNA polymerases
家族 Family familye.8.1.1 — DNA polymerase I
结构域编号 domain_idd2ex3h1
类 Class classa — All alpha proteins
折叠类型 Fold folda.263 — DNA terminal protein
超家族 Superfamily superfamilya.263.1 — DNA terminal protein
家族 Family familya.263.1.1 — DNA terminal protein
结构域编号 domain_idd2ex3i1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.55 — Ribonuclease H-like motif
超家族 Superfamily superfamilyc.55.3 — Ribonuclease H-like
家族 Family familyc.55.3.5 — DnaQ-like 3'-5' exonuclease
结构域编号 domain_idd2ex3i2
类 Class classe — Multi-domain proteins (alpha and beta)
折叠类型 Fold folde.8 — DNA/RNA polymerases
超家族 Superfamily superfamilye.8.1 — DNA/RNA polymerases
家族 Family familye.8.1.1 — DNA polymerase I
结构域编号 domain_idd2ex3j1
类 Class classa — All alpha proteins
折叠类型 Fold folda.263 — DNA terminal protein
超家族 Superfamily superfamilya.263.1 — DNA terminal protein
家族 Family familya.263.1.1 — DNA terminal protein
结构域编号 domain_idd2ex3k1
类 Class classc — Alpha and beta proteins (a/b)
折叠类型 Fold foldc.55 — Ribonuclease H-like motif
超家族 Superfamily superfamilyc.55.3 — Ribonuclease H-like
家族 Family familyc.55.3.5 — DnaQ-like 3'-5' exonuclease
结构域编号 domain_idd2ex3k2
类 Class classe — Multi-domain proteins (alpha and beta)
折叠类型 Fold folde.8 — DNA/RNA polymerases
超家族 Superfamily superfamilye.8.1 — DNA/RNA polymerases
家族 Family familye.8.1.1 — DNA polymerase I
结构域编号 domain_idd2ex3l1
类 Class classa — All alpha proteins
折叠类型 Fold folda.263 — DNA terminal protein
超家族 Superfamily superfamilya.263.1 — DNA terminal protein
家族 Family familya.263.1.1 — DNA terminal protein

CATH v4.4 (48 domains)

结构域编号 domain_id2ex3A01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily10 — Ribonuclease H-like superfamily/Ribonuclease H
结构域编号 domain_id2ex3A02
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1600 — Palm domain of DNA polymerase
同源超家族 H-superfamily homologous superfamily10 — B family DNA polymerase, palm domain
结构域编号 domain_id2ex3A03
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology1770 — TPR 1 domain of DNA polymerase
同源超家族 H-superfamily homologous superfamily10 — TPR 1 domain of DNA polymerase
结构域编号 domain_id2ex3A04
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily690 — B family DNA polymerase, finger domain
结构域编号 domain_id2ex3A05
类 Class class4 — Few Secondary Structures
架构 Architecture architecture10 — Irregular
拓扑 Topology topology80 — Rhinovirus 14, subunit 4
同源超家族 H-superfamily homologous superfamily20 — DNA polymerase; domain 5
结构域编号 domain_id2ex3A06
类 Class class4 — Few Secondary Structures
架构 Architecture architecture10 — Irregular
拓扑 Topology topology80 — Rhinovirus 14, subunit 4
同源超家族 H-superfamily homologous superfamily30 — DNA polymerase; domain 6
结构域编号 domain_id2ex3B01
类 Class class6 — Special
架构 Architecture architecture10 — Helix non-globular
拓扑 Topology topology250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily960
结构域编号 domain_id2ex3B02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1270 — Substrate Binding Domain Of Dnak; Chain:A; Domain 2
同源超家族 H-superfamily homologous superfamily230 — DNA terminal protein Gp3, priming domain
结构域编号 domain_id2ex3C01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily10 — Ribonuclease H-like superfamily/Ribonuclease H
结构域编号 domain_id2ex3C02
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1600 — Palm domain of DNA polymerase
同源超家族 H-superfamily homologous superfamily10 — B family DNA polymerase, palm domain
结构域编号 domain_id2ex3C03
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology1770 — TPR 1 domain of DNA polymerase
同源超家族 H-superfamily homologous superfamily10 — TPR 1 domain of DNA polymerase
结构域编号 domain_id2ex3C04
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily690 — B family DNA polymerase, finger domain
结构域编号 domain_id2ex3C05
类 Class class4 — Few Secondary Structures
架构 Architecture architecture10 — Irregular
拓扑 Topology topology80 — Rhinovirus 14, subunit 4
同源超家族 H-superfamily homologous superfamily20 — DNA polymerase; domain 5
结构域编号 domain_id2ex3C06
类 Class class4 — Few Secondary Structures
架构 Architecture architecture10 — Irregular
拓扑 Topology topology80 — Rhinovirus 14, subunit 4
同源超家族 H-superfamily homologous superfamily30 — DNA polymerase; domain 6
结构域编号 domain_id2ex3D01
类 Class class6 — Special
架构 Architecture architecture10 — Helix non-globular
拓扑 Topology topology250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily960
结构域编号 domain_id2ex3D02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1270 — Substrate Binding Domain Of Dnak; Chain:A; Domain 2
同源超家族 H-superfamily homologous superfamily230 — DNA terminal protein Gp3, priming domain
结构域编号 domain_id2ex3E01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily10 — Ribonuclease H-like superfamily/Ribonuclease H
结构域编号 domain_id2ex3E02
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1600 — Palm domain of DNA polymerase
同源超家族 H-superfamily homologous superfamily10 — B family DNA polymerase, palm domain
结构域编号 domain_id2ex3E03
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology1770 — TPR 1 domain of DNA polymerase
同源超家族 H-superfamily homologous superfamily10 — TPR 1 domain of DNA polymerase
结构域编号 domain_id2ex3E04
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily690 — B family DNA polymerase, finger domain
结构域编号 domain_id2ex3E05
类 Class class4 — Few Secondary Structures
架构 Architecture architecture10 — Irregular
拓扑 Topology topology80 — Rhinovirus 14, subunit 4
同源超家族 H-superfamily homologous superfamily20 — DNA polymerase; domain 5
结构域编号 domain_id2ex3E06
类 Class class4 — Few Secondary Structures
架构 Architecture architecture10 — Irregular
拓扑 Topology topology80 — Rhinovirus 14, subunit 4
同源超家族 H-superfamily homologous superfamily30 — DNA polymerase; domain 6
结构域编号 domain_id2ex3F01
类 Class class6 — Special
架构 Architecture architecture10 — Helix non-globular
拓扑 Topology topology250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily960
结构域编号 domain_id2ex3F02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1270 — Substrate Binding Domain Of Dnak; Chain:A; Domain 2
同源超家族 H-superfamily homologous superfamily230 — DNA terminal protein Gp3, priming domain
结构域编号 domain_id2ex3G01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily10 — Ribonuclease H-like superfamily/Ribonuclease H
结构域编号 domain_id2ex3G02
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1600 — Palm domain of DNA polymerase
同源超家族 H-superfamily homologous superfamily10 — B family DNA polymerase, palm domain
结构域编号 domain_id2ex3G03
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology1770 — TPR 1 domain of DNA polymerase
同源超家族 H-superfamily homologous superfamily10 — TPR 1 domain of DNA polymerase
结构域编号 domain_id2ex3G04
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily690 — B family DNA polymerase, finger domain
结构域编号 domain_id2ex3G05
类 Class class4 — Few Secondary Structures
架构 Architecture architecture10 — Irregular
拓扑 Topology topology80 — Rhinovirus 14, subunit 4
同源超家族 H-superfamily homologous superfamily20 — DNA polymerase; domain 5
结构域编号 domain_id2ex3G06
类 Class class4 — Few Secondary Structures
架构 Architecture architecture10 — Irregular
拓扑 Topology topology80 — Rhinovirus 14, subunit 4
同源超家族 H-superfamily homologous superfamily30 — DNA polymerase; domain 6
结构域编号 domain_id2ex3H01
类 Class class6 — Special
架构 Architecture architecture10 — Helix non-globular
拓扑 Topology topology250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily960
结构域编号 domain_id2ex3H02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1270 — Substrate Binding Domain Of Dnak; Chain:A; Domain 2
同源超家族 H-superfamily homologous superfamily230 — DNA terminal protein Gp3, priming domain
结构域编号 domain_id2ex3I01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily10 — Ribonuclease H-like superfamily/Ribonuclease H
结构域编号 domain_id2ex3I02
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1600 — Palm domain of DNA polymerase
同源超家族 H-superfamily homologous superfamily10 — B family DNA polymerase, palm domain
结构域编号 domain_id2ex3I03
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology1770 — TPR 1 domain of DNA polymerase
同源超家族 H-superfamily homologous superfamily10 — TPR 1 domain of DNA polymerase
结构域编号 domain_id2ex3I04
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily690 — B family DNA polymerase, finger domain
结构域编号 domain_id2ex3I05
类 Class class4 — Few Secondary Structures
架构 Architecture architecture10 — Irregular
拓扑 Topology topology80 — Rhinovirus 14, subunit 4
同源超家族 H-superfamily homologous superfamily20 — DNA polymerase; domain 5
结构域编号 domain_id2ex3I06
类 Class class4 — Few Secondary Structures
架构 Architecture architecture10 — Irregular
拓扑 Topology topology80 — Rhinovirus 14, subunit 4
同源超家族 H-superfamily homologous superfamily30 — DNA polymerase; domain 6
结构域编号 domain_id2ex3J01
类 Class class6 — Special
架构 Architecture architecture10 — Helix non-globular
拓扑 Topology topology250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily960
结构域编号 domain_id2ex3J02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1270 — Substrate Binding Domain Of Dnak; Chain:A; Domain 2
同源超家族 H-superfamily homologous superfamily230 — DNA terminal protein Gp3, priming domain
结构域编号 domain_id2ex3K01
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology420 — Nucleotidyltransferase; domain 5
同源超家族 H-superfamily homologous superfamily10 — Ribonuclease H-like superfamily/Ribonuclease H
结构域编号 domain_id2ex3K02
类 Class class3 — Alpha Beta
架构 Architecture architecture90 — Alpha-Beta Complex
拓扑 Topology topology1600 — Palm domain of DNA polymerase
同源超家族 H-superfamily homologous superfamily10 — B family DNA polymerase, palm domain
结构域编号 domain_id2ex3K03
类 Class class3 — Alpha Beta
架构 Architecture architecture30 — 2-Layer Sandwich
拓扑 Topology topology1770 — TPR 1 domain of DNA polymerase
同源超家族 H-superfamily homologous superfamily10 — TPR 1 domain of DNA polymerase
结构域编号 domain_id2ex3K04
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily690 — B family DNA polymerase, finger domain
结构域编号 domain_id2ex3K05
类 Class class4 — Few Secondary Structures
架构 Architecture architecture10 — Irregular
拓扑 Topology topology80 — Rhinovirus 14, subunit 4
同源超家族 H-superfamily homologous superfamily20 — DNA polymerase; domain 5
结构域编号 domain_id2ex3K06
类 Class class4 — Few Secondary Structures
架构 Architecture architecture10 — Irregular
拓扑 Topology topology80 — Rhinovirus 14, subunit 4
同源超家族 H-superfamily homologous superfamily30 — DNA polymerase; domain 6
结构域编号 domain_id2ex3L01
类 Class class6 — Special
架构 Architecture architecture10 — Helix non-globular
拓扑 Topology topology250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily960
结构域编号 domain_id2ex3L02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology1270 — Substrate Binding Domain Of Dnak; Chain:A; Domain 2
同源超家族 H-superfamily homologous superfamily230 — DNA terminal protein Gp3, priming domain

7. 引用文献 (1)

8. 文件与曲线 (10)