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2fug

Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus

Method: X-RAY DIFFRACTION Dmax: 288.0 Å Quality: GOOD
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1. Protein Identity and Related Structures Protein Identity & Related Structures

NADH-quinone oxidoreductase chain 1

OrganismNot specified

UniProt Q56222

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain 1； UniProt 1–438 Fragment：Hydrophilic domain NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
2 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain A； UniProt 1–438 Fragment：Hydrophilic domain NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
3 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain J； UniProt 1–438 Fragment：Hydrophilic domain NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
4 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain S； UniProt 1–438 Fragment：Hydrophilic domain NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 2FUG Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2YBB Fitted model for bovine mitochondrial supercomplex I1III2IV1 by single particle cryo-EM (EMD-1876) Deposited 2011-03-02 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric
Chain 1 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 7 FES FE2/S2 (INORGANIC) CLUSTER × 4 CA CALCIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 5 SMA STIGMATELLIN A × 2 UQ1 UBIQUINONE-1 × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 4 HEA HEME-A × 2 CU COPPER (II) ION × 3 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE；pH 7.7；10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 70, TEMPERATURE- 95, INSTRUMENT- CUSTOM-MADE HAND PLUNGER, METHOD- ONE- SIDED BLOTTING FOR 26 - 30 SECONDS BEFORE PLUNGING, DETAILS- SAMPLE WAS ADSORBED TO CARBON FILM FOR 30 SECONDS BEFORE BLOTTING
Resolution 19.00 Å
3I9V Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 2 mol/ASU Deposited 2009-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 1 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 MN MANGANESE (II) ION × 7 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M MnCl2, 6% PEG4000, 2.5% PEG400, 20 mM NAD+, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.283
3I9V Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 2 mol/ASU Deposited 2009-07-13 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 MN MANGANESE (II) ION × 5 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M MnCl2, 6% PEG4000, 2.5% PEG400, 20 mM NAD+, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.283
3IAM Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, reduced, 2 mol/ASU, with bound NADH Deposited 2009-07-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 1 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.05 M NaCl, 0.1 M MgCl2, 7% PEG4000, 3 mM NADH, 5 mM sodium dithionite, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.262
3IAM Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, reduced, 2 mol/ASU, with bound NADH Deposited 2009-07-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.05 M NaCl, 0.1 M MgCl2, 7% PEG4000, 3 mM NADH, 5 mM sodium dithionite, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.262
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 1 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain A 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain J 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different construct Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain S 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3M9S Crystal structure of respiratory complex I from Thermus thermophilus Deposited 2010-03-22 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain 1 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；16% PEG 4000, 100 mM Bis-Tris, 100 mM KCl, 100 mM glutaric acid and 2.04 mM n-octyl- -maltoside fluorinated, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 4.50 Å
3M9S Crystal structure of respiratory complex I from Thermus thermophilus Deposited 2010-03-22 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain A 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；16% PEG 4000, 100 mM Bis-Tris, 100 mM KCl, 100 mM glutaric acid and 2.04 mM n-octyl- -maltoside fluorinated, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 4.50 Å
4HEA Crystal structure of the entire respiratory complex I from Thermus thermophilus Deposited 2012-10-03 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 1 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 19% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 2.2 mM FOS-CHOLINE-8 fluorinated , VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 24% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201, VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.30 Å R-free 0.239
4HEA Crystal structure of the entire respiratory complex I from Thermus thermophilus Deposited 2012-10-03 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain B 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 19% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 2.2 mM FOS-CHOLINE-8 fluorinated , VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 24% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201, VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.30 Å R-free 0.239
6I0D Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone Deposited 2018-10-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 1 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 DCQ 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201
Resolution 3.60 Å R-free 0.225
6I0D Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone Deposited 2018-10-25 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain B 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 DCQ 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201
Resolution 3.60 Å R-free 0.225
6I1P Respiratory complex I from Thermus thermophilus with bound NADH Deposited 2018-10-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 1 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.01 mM n-tetradecyl-beta-D-maltopyranoside
Resolution 3.21 Å R-free 0.231
6I1P Respiratory complex I from Thermus thermophilus with bound NADH Deposited 2018-10-29 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain B 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.01 mM n-tetradecyl-beta-D-maltopyranoside
Resolution 3.21 Å R-free 0.231
6Q8O Respiratory complex I from Thermus thermophilus with bound Piericidin A Deposited 2018-12-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 1 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQH Piericidin A × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 24% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 0.59 mM n-undecyl-beta-maltoside
Resolution 3.60 Å R-free 0.235
6Q8O Respiratory complex I from Thermus thermophilus with bound Piericidin A Deposited 2018-12-15 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain B 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQH Piericidin A × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 24% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 0.59 mM n-undecyl-beta-maltoside
Resolution 3.60 Å R-free 0.235
6Q8W Respiratory complex I from Thermus thermophilus with bound Aureothin. Deposited 2018-12-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 1 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQW Aureothin × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 25% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 7.6 mM 4-cyclohexyl-1-butyl-beta-D-maltoside
Resolution 3.40 Å R-free 0.234
6Q8W Respiratory complex I from Thermus thermophilus with bound Aureothin. Deposited 2018-12-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain B 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQW Aureothin × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 25% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 7.6 mM 4-cyclohexyl-1-butyl-beta-D-maltoside
Resolution 3.40 Å R-free 0.234
6Q8X Respiratory complex I from Thermus thermophilus with bound Pyridaben. Deposited 2018-12-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 1 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQK Pyridaben × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 21% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 1.02 mM octyl-maltoside fluorinated
Resolution 3.51 Å R-free 0.232
6Q8X Respiratory complex I from Thermus thermophilus with bound Pyridaben. Deposited 2018-12-16 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain B 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQK Pyridaben × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 21% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 1.02 mM octyl-maltoside fluorinated
Resolution 3.51 Å R-free 0.232
6Y11 Respiratory complex I from Thermus thermophilus Deposited 2020-02-10 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 1 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM
Resolution 3.11 Å R-free 0.229
6Y11 Respiratory complex I from Thermus thermophilus Deposited 2020-02-10 Different construct Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain B 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM
Resolution 3.11 Å R-free 0.229
6ZIY Respiratory complex I from Thermus thermophilus, NADH dataset, major state Deposited 2020-06-26 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 1 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.25 Å
6ZJL Respiratory complex I from Thermus thermophilus, NAD+ dataset, major state Deposited 2020-06-29 Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 1 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
6ZJN Respiratory complex I from Thermus thermophilus, NADH dataset, minor state Deposited 2020-06-29 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 1 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.10 Å
6ZJY Respiratory complex I from Thermus thermophilus, NAD+ dataset, minor state Deposited 2020-06-29 Different construct Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 1 1–438（438 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.50 Å

16 other PDB entries and 29 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NQO1_THET8
Isoform —
PDB entities 1
Chains and sequence ranges Author chain 1； PDBConstruct 1–438； UniProt 1–438 Author chain A； PDBConstruct 1–438； UniProt 1–438 Author chain J； PDBConstruct 1–438； UniProt 1–438 Author chain S； PDBConstruct 1–438； UniProt 1–438

NADH-quinone oxidoreductase chain 2

OrganismNot specified

UniProt Q56221

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain 2； UniProt 1–181 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
2 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain B； UniProt 1–181 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
3 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain K； UniProt 1–181 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
4 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain T； UniProt 1–181 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 2FUG Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2YBB Fitted model for bovine mitochondrial supercomplex I1III2IV1 by single particle cryo-EM (EMD-1876) Deposited 2011-03-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric
Chain 2 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 7 FES FE2/S2 (INORGANIC) CLUSTER × 4 CA CALCIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 5 SMA STIGMATELLIN A × 2 UQ1 UBIQUINONE-1 × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 4 HEA HEME-A × 2 CU COPPER (II) ION × 3 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE；pH 7.7；10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 70, TEMPERATURE- 95, INSTRUMENT- CUSTOM-MADE HAND PLUNGER, METHOD- ONE- SIDED BLOTTING FOR 26 - 30 SECONDS BEFORE PLUNGING, DETAILS- SAMPLE WAS ADSORBED TO CARBON FILM FOR 30 SECONDS BEFORE BLOTTING
Resolution 19.00 Å
3I9V Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 2 mol/ASU Deposited 2009-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 2 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 MN MANGANESE (II) ION × 7 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M MnCl2, 6% PEG4000, 2.5% PEG400, 20 mM NAD+, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.283
3I9V Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 2 mol/ASU Deposited 2009-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 MN MANGANESE (II) ION × 5 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M MnCl2, 6% PEG4000, 2.5% PEG400, 20 mM NAD+, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.283
3IAM Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, reduced, 2 mol/ASU, with bound NADH Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 2 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.05 M NaCl, 0.1 M MgCl2, 7% PEG4000, 3 mM NADH, 5 mM sodium dithionite, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.262
3IAM Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, reduced, 2 mol/ASU, with bound NADH Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.05 M NaCl, 0.1 M MgCl2, 7% PEG4000, 3 mM NADH, 5 mM sodium dithionite, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.262
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 2 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain B 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain K 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain T 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3M9S Crystal structure of respiratory complex I from Thermus thermophilus Deposited 2010-03-22 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain 2 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；16% PEG 4000, 100 mM Bis-Tris, 100 mM KCl, 100 mM glutaric acid and 2.04 mM n-octyl- -maltoside fluorinated, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 4.50 Å
3M9S Crystal structure of respiratory complex I from Thermus thermophilus Deposited 2010-03-22 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain B 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；16% PEG 4000, 100 mM Bis-Tris, 100 mM KCl, 100 mM glutaric acid and 2.04 mM n-octyl- -maltoside fluorinated, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 4.50 Å
4HEA Crystal structure of the entire respiratory complex I from Thermus thermophilus Deposited 2012-10-03 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 2 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 19% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 2.2 mM FOS-CHOLINE-8 fluorinated , VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 24% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201, VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.30 Å R-free 0.239
4HEA Crystal structure of the entire respiratory complex I from Thermus thermophilus Deposited 2012-10-03 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain C 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 19% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 2.2 mM FOS-CHOLINE-8 fluorinated , VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 24% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201, VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.30 Å R-free 0.239
6I0D Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone Deposited 2018-10-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 2 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 DCQ 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201
Resolution 3.60 Å R-free 0.225
6I0D Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone Deposited 2018-10-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain C 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 DCQ 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201
Resolution 3.60 Å R-free 0.225
6I1P Respiratory complex I from Thermus thermophilus with bound NADH Deposited 2018-10-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 2 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.01 mM n-tetradecyl-beta-D-maltopyranoside
Resolution 3.21 Å R-free 0.231
6I1P Respiratory complex I from Thermus thermophilus with bound NADH Deposited 2018-10-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain C 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.01 mM n-tetradecyl-beta-D-maltopyranoside
Resolution 3.21 Å R-free 0.231
6Q8O Respiratory complex I from Thermus thermophilus with bound Piericidin A Deposited 2018-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 2 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQH Piericidin A × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 24% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 0.59 mM n-undecyl-beta-maltoside
Resolution 3.60 Å R-free 0.235
6Q8O Respiratory complex I from Thermus thermophilus with bound Piericidin A Deposited 2018-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain C 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQH Piericidin A × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 24% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 0.59 mM n-undecyl-beta-maltoside
Resolution 3.60 Å R-free 0.235
6Q8W Respiratory complex I from Thermus thermophilus with bound Aureothin. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 2 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQW Aureothin × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 25% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 7.6 mM 4-cyclohexyl-1-butyl-beta-D-maltoside
Resolution 3.40 Å R-free 0.234
6Q8W Respiratory complex I from Thermus thermophilus with bound Aureothin. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain C 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQW Aureothin × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 25% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 7.6 mM 4-cyclohexyl-1-butyl-beta-D-maltoside
Resolution 3.40 Å R-free 0.234
6Q8X Respiratory complex I from Thermus thermophilus with bound Pyridaben. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 2 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQK Pyridaben × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 21% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 1.02 mM octyl-maltoside fluorinated
Resolution 3.51 Å R-free 0.232
6Q8X Respiratory complex I from Thermus thermophilus with bound Pyridaben. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain C 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQK Pyridaben × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 21% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 1.02 mM octyl-maltoside fluorinated
Resolution 3.51 Å R-free 0.232
6Y11 Respiratory complex I from Thermus thermophilus Deposited 2020-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 2 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM
Resolution 3.11 Å R-free 0.229
6Y11 Respiratory complex I from Thermus thermophilus Deposited 2020-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain C 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM
Resolution 3.11 Å R-free 0.229
6ZIY Respiratory complex I from Thermus thermophilus, NADH dataset, major state Deposited 2020-06-26 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 2 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.25 Å
6ZJL Respiratory complex I from Thermus thermophilus, NAD+ dataset, major state Deposited 2020-06-29 Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 2 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
6ZJN Respiratory complex I from Thermus thermophilus, NADH dataset, minor state Deposited 2020-06-29 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 2 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.10 Å
6ZJY Respiratory complex I from Thermus thermophilus, NAD+ dataset, minor state Deposited 2020-06-29 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 2 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.50 Å

16 other PDB entries and 29 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NQO2_THET8
Isoform —
PDB entities 2
Chains and sequence ranges Author chain 2； PDBConstruct 1–181； UniProt 1–181 Author chain B； PDBConstruct 1–181； UniProt 1–181 Author chain K； PDBConstruct 1–181； UniProt 1–181 Author chain T； PDBConstruct 1–181； UniProt 1–181

NADH-quinone oxidoreductase chain 3

OrganismNot specified

UniProt Q56223

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain 3； UniProt 1–783 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
2 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain C； UniProt 1–783 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
3 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain L； UniProt 1–783 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
4 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain U； UniProt 1–783 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 2FUG Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2YBB Fitted model for bovine mitochondrial supercomplex I1III2IV1 by single particle cryo-EM (EMD-1876) Deposited 2011-03-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric
Chain 3 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 7 FES FE2/S2 (INORGANIC) CLUSTER × 4 CA CALCIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 5 SMA STIGMATELLIN A × 2 UQ1 UBIQUINONE-1 × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 4 HEA HEME-A × 2 CU COPPER (II) ION × 3 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE；pH 7.7；10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 70, TEMPERATURE- 95, INSTRUMENT- CUSTOM-MADE HAND PLUNGER, METHOD- ONE- SIDED BLOTTING FOR 26 - 30 SECONDS BEFORE PLUNGING, DETAILS- SAMPLE WAS ADSORBED TO CARBON FILM FOR 30 SECONDS BEFORE BLOTTING
Resolution 19.00 Å
3I9V Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 2 mol/ASU Deposited 2009-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 3 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 MN MANGANESE (II) ION × 7 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M MnCl2, 6% PEG4000, 2.5% PEG400, 20 mM NAD+, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.283
3I9V Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 2 mol/ASU Deposited 2009-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 MN MANGANESE (II) ION × 5 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M MnCl2, 6% PEG4000, 2.5% PEG400, 20 mM NAD+, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.283
3IAM Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, reduced, 2 mol/ASU, with bound NADH Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 3 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.05 M NaCl, 0.1 M MgCl2, 7% PEG4000, 3 mM NADH, 5 mM sodium dithionite, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.262
3IAM Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, reduced, 2 mol/ASU, with bound NADH Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.05 M NaCl, 0.1 M MgCl2, 7% PEG4000, 3 mM NADH, 5 mM sodium dithionite, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.262
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 3 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain C 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain L 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain U 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3M9S Crystal structure of respiratory complex I from Thermus thermophilus Deposited 2010-03-22 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain 3 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；16% PEG 4000, 100 mM Bis-Tris, 100 mM KCl, 100 mM glutaric acid and 2.04 mM n-octyl- -maltoside fluorinated, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 4.50 Å
3M9S Crystal structure of respiratory complex I from Thermus thermophilus Deposited 2010-03-22 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain C 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；16% PEG 4000, 100 mM Bis-Tris, 100 mM KCl, 100 mM glutaric acid and 2.04 mM n-octyl- -maltoside fluorinated, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 4.50 Å
4HEA Crystal structure of the entire respiratory complex I from Thermus thermophilus Deposited 2012-10-03 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 3 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 19% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 2.2 mM FOS-CHOLINE-8 fluorinated , VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 24% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201, VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.30 Å R-free 0.239
4HEA Crystal structure of the entire respiratory complex I from Thermus thermophilus Deposited 2012-10-03 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain D 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 19% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 2.2 mM FOS-CHOLINE-8 fluorinated , VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 24% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201, VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.30 Å R-free 0.239
6I0D Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone Deposited 2018-10-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 3 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 DCQ 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201
Resolution 3.60 Å R-free 0.225
6I0D Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone Deposited 2018-10-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain D 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 DCQ 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201
Resolution 3.60 Å R-free 0.225
6I1P Respiratory complex I from Thermus thermophilus with bound NADH Deposited 2018-10-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 3 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.01 mM n-tetradecyl-beta-D-maltopyranoside
Resolution 3.21 Å R-free 0.231
6I1P Respiratory complex I from Thermus thermophilus with bound NADH Deposited 2018-10-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain D 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.01 mM n-tetradecyl-beta-D-maltopyranoside
Resolution 3.21 Å R-free 0.231
6Q8O Respiratory complex I from Thermus thermophilus with bound Piericidin A Deposited 2018-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 3 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQH Piericidin A × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 24% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 0.59 mM n-undecyl-beta-maltoside
Resolution 3.60 Å R-free 0.235
6Q8O Respiratory complex I from Thermus thermophilus with bound Piericidin A Deposited 2018-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain D 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQH Piericidin A × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 24% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 0.59 mM n-undecyl-beta-maltoside
Resolution 3.60 Å R-free 0.235
6Q8W Respiratory complex I from Thermus thermophilus with bound Aureothin. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 3 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQW Aureothin × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 25% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 7.6 mM 4-cyclohexyl-1-butyl-beta-D-maltoside
Resolution 3.40 Å R-free 0.234
6Q8W Respiratory complex I from Thermus thermophilus with bound Aureothin. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain D 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQW Aureothin × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 25% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 7.6 mM 4-cyclohexyl-1-butyl-beta-D-maltoside
Resolution 3.40 Å R-free 0.234
6Q8X Respiratory complex I from Thermus thermophilus with bound Pyridaben. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 3 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQK Pyridaben × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 21% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 1.02 mM octyl-maltoside fluorinated
Resolution 3.51 Å R-free 0.232
6Q8X Respiratory complex I from Thermus thermophilus with bound Pyridaben. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain D 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQK Pyridaben × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 21% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 1.02 mM octyl-maltoside fluorinated
Resolution 3.51 Å R-free 0.232
6Y11 Respiratory complex I from Thermus thermophilus Deposited 2020-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 3 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM
Resolution 3.11 Å R-free 0.229
6Y11 Respiratory complex I from Thermus thermophilus Deposited 2020-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain D 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM
Resolution 3.11 Å R-free 0.229
6ZIY Respiratory complex I from Thermus thermophilus, NADH dataset, major state Deposited 2020-06-26 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 3 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.25 Å
6ZJL Respiratory complex I from Thermus thermophilus, NAD+ dataset, major state Deposited 2020-06-29 Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 3 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
6ZJN Respiratory complex I from Thermus thermophilus, NADH dataset, minor state Deposited 2020-06-29 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 3 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.10 Å
6ZJY Respiratory complex I from Thermus thermophilus, NAD+ dataset, minor state Deposited 2020-06-29 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 3 1–783（783 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.50 Å

16 other PDB entries and 29 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NQO3_THET8
Isoform —
PDB entities 3
Chains and sequence ranges Author chain 3； PDBConstruct 1–783； UniProt 1–783 Author chain C； PDBConstruct 1–783； UniProt 1–783 Author chain L； PDBConstruct 1–783； UniProt 1–783 Author chain U； PDBConstruct 1–783； UniProt 1–783

NADH-quinone oxidoreductase chain 4

OrganismNot specified

UniProt Q56220

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain 4； UniProt 1–409 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
2 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain D； UniProt 1–409 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
3 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain M； UniProt 1–409 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
4 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain V； UniProt 1–409 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 2FUG Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2YBB Fitted model for bovine mitochondrial supercomplex I1III2IV1 by single particle cryo-EM (EMD-1876) Deposited 2011-03-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric
Chain 4 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 7 FES FE2/S2 (INORGANIC) CLUSTER × 4 CA CALCIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 5 SMA STIGMATELLIN A × 2 UQ1 UBIQUINONE-1 × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 4 HEA HEME-A × 2 CU COPPER (II) ION × 3 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE；pH 7.7；10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 70, TEMPERATURE- 95, INSTRUMENT- CUSTOM-MADE HAND PLUNGER, METHOD- ONE- SIDED BLOTTING FOR 26 - 30 SECONDS BEFORE PLUNGING, DETAILS- SAMPLE WAS ADSORBED TO CARBON FILM FOR 30 SECONDS BEFORE BLOTTING
Resolution 19.00 Å
3I9V Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 2 mol/ASU Deposited 2009-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 4 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 MN MANGANESE (II) ION × 7 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M MnCl2, 6% PEG4000, 2.5% PEG400, 20 mM NAD+, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.283
3I9V Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 2 mol/ASU Deposited 2009-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain D 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 MN MANGANESE (II) ION × 5 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M MnCl2, 6% PEG4000, 2.5% PEG400, 20 mM NAD+, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.283
3IAM Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, reduced, 2 mol/ASU, with bound NADH Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 4 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.05 M NaCl, 0.1 M MgCl2, 7% PEG4000, 3 mM NADH, 5 mM sodium dithionite, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.262
3IAM Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, reduced, 2 mol/ASU, with bound NADH Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain D 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.05 M NaCl, 0.1 M MgCl2, 7% PEG4000, 3 mM NADH, 5 mM sodium dithionite, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.262
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 4 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain D 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain M 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain V 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3M9S Crystal structure of respiratory complex I from Thermus thermophilus Deposited 2010-03-22 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain 4 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；16% PEG 4000, 100 mM Bis-Tris, 100 mM KCl, 100 mM glutaric acid and 2.04 mM n-octyl- -maltoside fluorinated, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 4.50 Å
3M9S Crystal structure of respiratory complex I from Thermus thermophilus Deposited 2010-03-22 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain D 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；16% PEG 4000, 100 mM Bis-Tris, 100 mM KCl, 100 mM glutaric acid and 2.04 mM n-octyl- -maltoside fluorinated, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 4.50 Å
4HEA Crystal structure of the entire respiratory complex I from Thermus thermophilus Deposited 2012-10-03 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 4 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 19% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 2.2 mM FOS-CHOLINE-8 fluorinated , VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 24% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201, VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.30 Å R-free 0.239
4HEA Crystal structure of the entire respiratory complex I from Thermus thermophilus Deposited 2012-10-03 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain E 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 19% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 2.2 mM FOS-CHOLINE-8 fluorinated , VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 24% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201, VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.30 Å R-free 0.239
6I0D Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone Deposited 2018-10-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 4 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 DCQ 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201
Resolution 3.60 Å R-free 0.225
6I0D Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone Deposited 2018-10-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain E 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 DCQ 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201
Resolution 3.60 Å R-free 0.225
6I1P Respiratory complex I from Thermus thermophilus with bound NADH Deposited 2018-10-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 4 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.01 mM n-tetradecyl-beta-D-maltopyranoside
Resolution 3.21 Å R-free 0.231
6I1P Respiratory complex I from Thermus thermophilus with bound NADH Deposited 2018-10-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain E 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.01 mM n-tetradecyl-beta-D-maltopyranoside
Resolution 3.21 Å R-free 0.231
6Q8O Respiratory complex I from Thermus thermophilus with bound Piericidin A Deposited 2018-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 4 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQH Piericidin A × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 24% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 0.59 mM n-undecyl-beta-maltoside
Resolution 3.60 Å R-free 0.235
6Q8O Respiratory complex I from Thermus thermophilus with bound Piericidin A Deposited 2018-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain E 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQH Piericidin A × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 24% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 0.59 mM n-undecyl-beta-maltoside
Resolution 3.60 Å R-free 0.235
6Q8W Respiratory complex I from Thermus thermophilus with bound Aureothin. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 4 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQW Aureothin × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 25% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 7.6 mM 4-cyclohexyl-1-butyl-beta-D-maltoside
Resolution 3.40 Å R-free 0.234
6Q8W Respiratory complex I from Thermus thermophilus with bound Aureothin. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain E 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQW Aureothin × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 25% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 7.6 mM 4-cyclohexyl-1-butyl-beta-D-maltoside
Resolution 3.40 Å R-free 0.234
6Q8X Respiratory complex I from Thermus thermophilus with bound Pyridaben. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 4 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQK Pyridaben × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 21% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 1.02 mM octyl-maltoside fluorinated
Resolution 3.51 Å R-free 0.232
6Q8X Respiratory complex I from Thermus thermophilus with bound Pyridaben. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain E 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQK Pyridaben × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 21% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 1.02 mM octyl-maltoside fluorinated
Resolution 3.51 Å R-free 0.232
6Y11 Respiratory complex I from Thermus thermophilus Deposited 2020-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 4 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM
Resolution 3.11 Å R-free 0.229
6Y11 Respiratory complex I from Thermus thermophilus Deposited 2020-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain E 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM
Resolution 3.11 Å R-free 0.229
6ZIY Respiratory complex I from Thermus thermophilus, NADH dataset, major state Deposited 2020-06-26 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 4 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.25 Å
6ZJL Respiratory complex I from Thermus thermophilus, NAD+ dataset, major state Deposited 2020-06-29 Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 4 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
6ZJN Respiratory complex I from Thermus thermophilus, NADH dataset, minor state Deposited 2020-06-29 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 4 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.10 Å
6ZJY Respiratory complex I from Thermus thermophilus, NAD+ dataset, minor state Deposited 2020-06-29 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 4 1–409（409 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.50 Å

16 other PDB entries and 29 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NQO4_THET8
Isoform —
PDB entities 4
Chains and sequence ranges Author chain 4； PDBConstruct 1–409； UniProt 1–409 Author chain D； PDBConstruct 1–409； UniProt 1–409 Author chain M； PDBConstruct 1–409； UniProt 1–409 Author chain V； PDBConstruct 1–409； UniProt 1–409

NADH-quinone oxidoreductase chain 5

OrganismNot specified

UniProt Q56219

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain 5； UniProt 1–207 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
2 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain E； UniProt 1–207 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
3 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain N； UniProt 1–207 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
4 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain W； UniProt 1–207 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 2FUG Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2YBB Fitted model for bovine mitochondrial supercomplex I1III2IV1 by single particle cryo-EM (EMD-1876) Deposited 2011-03-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric
Chain 5 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 7 FES FE2/S2 (INORGANIC) CLUSTER × 4 CA CALCIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 5 SMA STIGMATELLIN A × 2 UQ1 UBIQUINONE-1 × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 4 HEA HEME-A × 2 CU COPPER (II) ION × 3 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE；pH 7.7；10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 70, TEMPERATURE- 95, INSTRUMENT- CUSTOM-MADE HAND PLUNGER, METHOD- ONE- SIDED BLOTTING FOR 26 - 30 SECONDS BEFORE PLUNGING, DETAILS- SAMPLE WAS ADSORBED TO CARBON FILM FOR 30 SECONDS BEFORE BLOTTING
Resolution 19.00 Å
3I9V Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 2 mol/ASU Deposited 2009-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 5 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 MN MANGANESE (II) ION × 7 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M MnCl2, 6% PEG4000, 2.5% PEG400, 20 mM NAD+, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.283
3I9V Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 2 mol/ASU Deposited 2009-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 MN MANGANESE (II) ION × 5 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M MnCl2, 6% PEG4000, 2.5% PEG400, 20 mM NAD+, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.283
3IAM Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, reduced, 2 mol/ASU, with bound NADH Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 5 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.05 M NaCl, 0.1 M MgCl2, 7% PEG4000, 3 mM NADH, 5 mM sodium dithionite, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.262
3IAM Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, reduced, 2 mol/ASU, with bound NADH Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.05 M NaCl, 0.1 M MgCl2, 7% PEG4000, 3 mM NADH, 5 mM sodium dithionite, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.262
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 5 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain E 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain N 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain W 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3M9S Crystal structure of respiratory complex I from Thermus thermophilus Deposited 2010-03-22 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain 5 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；16% PEG 4000, 100 mM Bis-Tris, 100 mM KCl, 100 mM glutaric acid and 2.04 mM n-octyl- -maltoside fluorinated, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 4.50 Å
3M9S Crystal structure of respiratory complex I from Thermus thermophilus Deposited 2010-03-22 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain E 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；16% PEG 4000, 100 mM Bis-Tris, 100 mM KCl, 100 mM glutaric acid and 2.04 mM n-octyl- -maltoside fluorinated, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 4.50 Å
4HEA Crystal structure of the entire respiratory complex I from Thermus thermophilus Deposited 2012-10-03 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 5 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 19% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 2.2 mM FOS-CHOLINE-8 fluorinated , VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 24% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201, VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.30 Å R-free 0.239
4HEA Crystal structure of the entire respiratory complex I from Thermus thermophilus Deposited 2012-10-03 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain F 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 19% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 2.2 mM FOS-CHOLINE-8 fluorinated , VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 24% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201, VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.30 Å R-free 0.239
5B3P Nqo5 of the trypsin-resistant fragment (1-134) in P212121 form Deposited 2016-03-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–134（134 aa） Fragment：UNP residues 1-134
Not recorded CA CALCIUM ION × 3 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；200mM calcium chloride, 25% PEG 4000
Resolution 1.65 Å R-free 0.209
5B3Q Nqo5 of the trypsin-resistant fragment (1-134) in P63 form Deposited 2016-03-09 Different construct Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric
Chain A 1–134（134 aa） Fragment：UNP residues 1-134
Not recorded No recorded non-water small molecule X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 8；293 K；10% PEG 1000, 10% PEG 8000
Resolution 3.00 Å R-free 0.234
6I0D Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone Deposited 2018-10-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 5 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 DCQ 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201
Resolution 3.60 Å R-free 0.225
6I0D Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone Deposited 2018-10-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain F 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 DCQ 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201
Resolution 3.60 Å R-free 0.225
6I1P Respiratory complex I from Thermus thermophilus with bound NADH Deposited 2018-10-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 5 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.01 mM n-tetradecyl-beta-D-maltopyranoside
Resolution 3.21 Å R-free 0.231
6I1P Respiratory complex I from Thermus thermophilus with bound NADH Deposited 2018-10-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain F 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.01 mM n-tetradecyl-beta-D-maltopyranoside
Resolution 3.21 Å R-free 0.231
6Q8O Respiratory complex I from Thermus thermophilus with bound Piericidin A Deposited 2018-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 5 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQH Piericidin A × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 24% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 0.59 mM n-undecyl-beta-maltoside
Resolution 3.60 Å R-free 0.235
6Q8O Respiratory complex I from Thermus thermophilus with bound Piericidin A Deposited 2018-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain F 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQH Piericidin A × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 24% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 0.59 mM n-undecyl-beta-maltoside
Resolution 3.60 Å R-free 0.235
6Q8W Respiratory complex I from Thermus thermophilus with bound Aureothin. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 5 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQW Aureothin × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 25% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 7.6 mM 4-cyclohexyl-1-butyl-beta-D-maltoside
Resolution 3.40 Å R-free 0.234
6Q8W Respiratory complex I from Thermus thermophilus with bound Aureothin. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain F 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQW Aureothin × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 25% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 7.6 mM 4-cyclohexyl-1-butyl-beta-D-maltoside
Resolution 3.40 Å R-free 0.234
6Q8X Respiratory complex I from Thermus thermophilus with bound Pyridaben. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 5 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQK Pyridaben × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 21% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 1.02 mM octyl-maltoside fluorinated
Resolution 3.51 Å R-free 0.232
6Q8X Respiratory complex I from Thermus thermophilus with bound Pyridaben. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain F 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQK Pyridaben × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 21% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 1.02 mM octyl-maltoside fluorinated
Resolution 3.51 Å R-free 0.232
6Y11 Respiratory complex I from Thermus thermophilus Deposited 2020-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 5 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM
Resolution 3.11 Å R-free 0.229
6Y11 Respiratory complex I from Thermus thermophilus Deposited 2020-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain F 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM
Resolution 3.11 Å R-free 0.229
6ZIY Respiratory complex I from Thermus thermophilus, NADH dataset, major state Deposited 2020-06-26 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 5 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.25 Å
6ZJL Respiratory complex I from Thermus thermophilus, NAD+ dataset, major state Deposited 2020-06-29 Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 5 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
6ZJN Respiratory complex I from Thermus thermophilus, NADH dataset, minor state Deposited 2020-06-29 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 5 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.10 Å
6ZJY Respiratory complex I from Thermus thermophilus, NAD+ dataset, minor state Deposited 2020-06-29 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 5 1–207（207 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.50 Å

18 other PDB entries and 31 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NQO5_THET8
Isoform —
PDB entities 5
Chains and sequence ranges Author chain 5； PDBConstruct 1–207； UniProt 1–207 Author chain E； PDBConstruct 1–207； UniProt 1–207 Author chain N； PDBConstruct 1–207； UniProt 1–207 Author chain W； PDBConstruct 1–207； UniProt 1–207

NADH-quinone oxidoreductase chain 6

OrganismNot specified

UniProt Q56218

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain 6； UniProt 1–181 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
2 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain F； UniProt 1–181 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
3 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain O； UniProt 1–181 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
4 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain X； UniProt 1–181 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 9 × 1 （Q56224） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 2FUG Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2YBB Fitted model for bovine mitochondrial supercomplex I1III2IV1 by single particle cryo-EM (EMD-1876) Deposited 2011-03-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric
Chain 6 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 7 FES FE2/S2 (INORGANIC) CLUSTER × 4 CA CALCIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 5 SMA STIGMATELLIN A × 2 UQ1 UBIQUINONE-1 × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 4 HEA HEME-A × 2 CU COPPER (II) ION × 3 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE；pH 7.7；10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 70, TEMPERATURE- 95, INSTRUMENT- CUSTOM-MADE HAND PLUNGER, METHOD- ONE- SIDED BLOTTING FOR 26 - 30 SECONDS BEFORE PLUNGING, DETAILS- SAMPLE WAS ADSORBED TO CARBON FILM FOR 30 SECONDS BEFORE BLOTTING
Resolution 19.00 Å
3I9V Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 2 mol/ASU Deposited 2009-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 6 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 MN MANGANESE (II) ION × 7 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M MnCl2, 6% PEG4000, 2.5% PEG400, 20 mM NAD+, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.283
3I9V Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 2 mol/ASU Deposited 2009-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain F 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 MN MANGANESE (II) ION × 5 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M MnCl2, 6% PEG4000, 2.5% PEG400, 20 mM NAD+, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.283
3IAM Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, reduced, 2 mol/ASU, with bound NADH Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 6 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.05 M NaCl, 0.1 M MgCl2, 7% PEG4000, 3 mM NADH, 5 mM sodium dithionite, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.262
3IAM Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, reduced, 2 mol/ASU, with bound NADH Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain F 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.05 M NaCl, 0.1 M MgCl2, 7% PEG4000, 3 mM NADH, 5 mM sodium dithionite, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.262
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 6 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain F 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain O 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain X 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3M9S Crystal structure of respiratory complex I from Thermus thermophilus Deposited 2010-03-22 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain 6 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；16% PEG 4000, 100 mM Bis-Tris, 100 mM KCl, 100 mM glutaric acid and 2.04 mM n-octyl- -maltoside fluorinated, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 4.50 Å
3M9S Crystal structure of respiratory complex I from Thermus thermophilus Deposited 2010-03-22 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain F 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；16% PEG 4000, 100 mM Bis-Tris, 100 mM KCl, 100 mM glutaric acid and 2.04 mM n-octyl- -maltoside fluorinated, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 4.50 Å
4HEA Crystal structure of the entire respiratory complex I from Thermus thermophilus Deposited 2012-10-03 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 6 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 19% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 2.2 mM FOS-CHOLINE-8 fluorinated , VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 24% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201, VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.30 Å R-free 0.239
4HEA Crystal structure of the entire respiratory complex I from Thermus thermophilus Deposited 2012-10-03 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain G 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 19% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 2.2 mM FOS-CHOLINE-8 fluorinated , VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 24% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201, VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.30 Å R-free 0.239
6I0D Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone Deposited 2018-10-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 6 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 DCQ 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201
Resolution 3.60 Å R-free 0.225
6I0D Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone Deposited 2018-10-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain G 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 DCQ 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201
Resolution 3.60 Å R-free 0.225
6I1P Respiratory complex I from Thermus thermophilus with bound NADH Deposited 2018-10-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 6 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.01 mM n-tetradecyl-beta-D-maltopyranoside
Resolution 3.21 Å R-free 0.231
6I1P Respiratory complex I from Thermus thermophilus with bound NADH Deposited 2018-10-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain G 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.01 mM n-tetradecyl-beta-D-maltopyranoside
Resolution 3.21 Å R-free 0.231
6Q8O Respiratory complex I from Thermus thermophilus with bound Piericidin A Deposited 2018-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 6 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQH Piericidin A × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 24% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 0.59 mM n-undecyl-beta-maltoside
Resolution 3.60 Å R-free 0.235
6Q8O Respiratory complex I from Thermus thermophilus with bound Piericidin A Deposited 2018-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain G 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQH Piericidin A × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 24% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 0.59 mM n-undecyl-beta-maltoside
Resolution 3.60 Å R-free 0.235
6Q8W Respiratory complex I from Thermus thermophilus with bound Aureothin. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 6 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQW Aureothin × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 25% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 7.6 mM 4-cyclohexyl-1-butyl-beta-D-maltoside
Resolution 3.40 Å R-free 0.234
6Q8W Respiratory complex I from Thermus thermophilus with bound Aureothin. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain G 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQW Aureothin × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 25% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 7.6 mM 4-cyclohexyl-1-butyl-beta-D-maltoside
Resolution 3.40 Å R-free 0.234
6Q8X Respiratory complex I from Thermus thermophilus with bound Pyridaben. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 6 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQK Pyridaben × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 21% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 1.02 mM octyl-maltoside fluorinated
Resolution 3.51 Å R-free 0.232
6Q8X Respiratory complex I from Thermus thermophilus with bound Pyridaben. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain G 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQK Pyridaben × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 21% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 1.02 mM octyl-maltoside fluorinated
Resolution 3.51 Å R-free 0.232
6Y11 Respiratory complex I from Thermus thermophilus Deposited 2020-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 6 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM
Resolution 3.11 Å R-free 0.229
6Y11 Respiratory complex I from Thermus thermophilus Deposited 2020-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain G 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM
Resolution 3.11 Å R-free 0.229
6ZIY Respiratory complex I from Thermus thermophilus, NADH dataset, major state Deposited 2020-06-26 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 6 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.25 Å
6ZJL Respiratory complex I from Thermus thermophilus, NAD+ dataset, major state Deposited 2020-06-29 Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 6 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
6ZJN Respiratory complex I from Thermus thermophilus, NADH dataset, minor state Deposited 2020-06-29 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 6 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.10 Å
6ZJY Respiratory complex I from Thermus thermophilus, NAD+ dataset, minor state Deposited 2020-06-29 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 6 1–181（181 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.50 Å

16 other PDB entries and 29 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NQO6_THET8
Isoform —
PDB entities 6
Chains and sequence ranges Author chain 6； PDBConstruct 1–181； UniProt 1–181 Author chain F； PDBConstruct 1–181； UniProt 1–181 Author chain O； PDBConstruct 1–181； UniProt 1–181 Author chain X； PDBConstruct 1–181； UniProt 1–181

NADH-quinone oxidoreductase chain 9

OrganismNot specified

UniProt Q56224

State in the Current Structure

Assembly Oligomeric State Construct Mutations and Modifications Ligands, Ions and Associated Components Method and Experimental Conditions Structure Quality
1 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain 9； UniProt 1–182 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
2 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain G； UniProt 1–182 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
3 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain P； UniProt 1–182 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298
4 Protein heterocomplex Heteromer Protein × 8 PDB declaration: octameric（8） Consistent with protein copy count Chain Y； UniProt 1–182 Not recorded NADH-quinone oxidoreductase chain 1 × 1 （Q56222） NADH-quinone oxidoreductase chain 2 × 1 （Q56221） NADH-quinone oxidoreductase chain 3 × 1 （Q56223） NADH-quinone oxidoreductase chain 4 × 1 （Q56220） NADH-quinone oxidoreductase chain 5 × 1 （Q56219） NADH-quinone oxidoreductase chain 6 × 1 （Q56218） conserved hypothetical protein × 1 SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 FMN FLAVIN MONONUCLEOTIDE × 1 X-RAY DIFFRACTION X-ray crystallization conditions：VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1M HEPES pH 7.5, 0.4-0.5M NaCl, 0.1M CaCl2 and 8-10% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K Resolution 3.30 Å R-free 0.298

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB Difference from Current Entry 2FUG Assembly / Oligomeric State Construct Mutations and Modifications Ligands, Ions and Non-polymers Method and Experimental Conditions Structure Quality
2YBB Fitted model for bovine mitochondrial supercomplex I1III2IV1 by single particle cryo-EM (EMD-1876) Deposited 2011-03-02 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 48 PDB declaration: 48-meric
Chain 8 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 7 FES FE2/S2 (INORGANIC) CLUSTER × 4 CA CALCIUM ION × 1 HEM PROTOPORPHYRIN IX CONTAINING FE × 5 SMA STIGMATELLIN A × 2 UQ1 UBIQUINONE-1 × 2 HEC HEME C × 2 CDL CARDIOLIPIN × 4 HEA HEME-A × 2 CU COPPER (II) ION × 3 ZN ZINC ION × 1 ELECTRON MICROSCOPY
cryo-EM buffer 10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE；pH 7.7；10 MM KCL, 15 MM HEPES, RESIDUAL TREHALOSE
cryo-EM vitrification conditions Cryogen ETHANE；VITRIFICATION 1 -- CRYOGEN- ETHANE, HUMIDITY- 70, TEMPERATURE- 95, INSTRUMENT- CUSTOM-MADE HAND PLUNGER, METHOD- ONE- SIDED BLOTTING FOR 26 - 30 SECONDS BEFORE PLUNGING, DETAILS- SAMPLE WAS ADSORBED TO CARBON FILM FOR 30 SECONDS BEFORE BLOTTING
Resolution 19.00 Å
3I9V Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 2 mol/ASU Deposited 2009-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 9 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 MN MANGANESE (II) ION × 7 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M MnCl2, 6% PEG4000, 2.5% PEG400, 20 mM NAD+, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.283
3I9V Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 2 mol/ASU Deposited 2009-07-13 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 MN MANGANESE (II) ION × 5 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M MnCl2, 6% PEG4000, 2.5% PEG400, 20 mM NAD+, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.283
3IAM Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, reduced, 2 mol/ASU, with bound NADH Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 9 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 6 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.05 M NaCl, 0.1 M MgCl2, 7% PEG4000, 3 mM NADH, 5 mM sodium dithionite, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.262
3IAM Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, reduced, 2 mol/ASU, with bound NADH Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 MG MAGNESIUM ION × 4 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.05 M NaCl, 0.1 M MgCl2, 7% PEG4000, 3 mM NADH, 5 mM sodium dithionite, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.10 Å R-free 0.262
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain 9 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain G 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 3 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain P 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3IAS Crystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus, oxidized, 4 mol/ASU, re-refined to 3.15 angstrom resolution Deposited 2009-07-14 Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 4 Protein heterocomplex Heteromer；Protein × 8 PDB declaration: octameric
Chain Y 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 CA CALCIUM ION × 4 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 7.5；295 K；0.1 M HEPES pH 7.5, 0.4 M NaCl, 0.1 M CaCl2, 8% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.15 Å R-free 0.270
3M9S Crystal structure of respiratory complex I from Thermus thermophilus Deposited 2010-03-22 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain 9 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；16% PEG 4000, 100 mM Bis-Tris, 100 mM KCl, 100 mM glutaric acid and 2.04 mM n-octyl- -maltoside fluorinated, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 4.50 Å
3M9S Crystal structure of respiratory complex I from Thermus thermophilus Deposited 2010-03-22 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 13 PDB declaration: tridecameric
Chain G 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；16% PEG 4000, 100 mM Bis-Tris, 100 mM KCl, 100 mM glutaric acid and 2.04 mM n-octyl- -maltoside fluorinated, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 4.50 Å
4HEA Crystal structure of the entire respiratory complex I from Thermus thermophilus Deposited 2012-10-03 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 9 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 19% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 2.2 mM FOS-CHOLINE-8 fluorinated , VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 24% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201, VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.30 Å R-free 0.239
4HEA Crystal structure of the entire respiratory complex I from Thermus thermophilus Deposited 2012-10-03 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain O 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 19% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 2.2 mM FOS-CHOLINE-8 fluorinated , VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 24% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201, VAPOR DIFFUSION, SITTING DROP, temperature 295K
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM, VAPOR DIFFUSION, SITTING DROP, temperature 295K
Resolution 3.30 Å R-free 0.239
6I0D Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone Deposited 2018-10-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 9 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 DCQ 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201
Resolution 3.60 Å R-free 0.225
6I0D Respiratory complex I from Thermus thermophilus with bound Decyl-Ubiquinone Deposited 2018-10-25 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain O 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 DCQ 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 50 mM NDSB-201
Resolution 3.60 Å R-free 0.225
6I1P Respiratory complex I from Thermus thermophilus with bound NADH Deposited 2018-10-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 9 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.01 mM n-tetradecyl-beta-D-maltopyranoside
Resolution 3.21 Å R-free 0.231
6I1P Respiratory complex I from Thermus thermophilus with bound NADH Deposited 2018-10-29 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain O 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 23% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.01 mM n-tetradecyl-beta-D-maltopyranoside
Resolution 3.21 Å R-free 0.231
6Q8O Respiratory complex I from Thermus thermophilus with bound Piericidin A Deposited 2018-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 9 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQH Piericidin A × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 24% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 0.59 mM n-undecyl-beta-maltoside
Resolution 3.60 Å R-free 0.235
6Q8O Respiratory complex I from Thermus thermophilus with bound Piericidin A Deposited 2018-12-15 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain O 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQH Piericidin A × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 24% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 0.59 mM n-undecyl-beta-maltoside
Resolution 3.60 Å R-free 0.235
6Q8W Respiratory complex I from Thermus thermophilus with bound Aureothin. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 9 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQW Aureothin × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 25% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 7.6 mM 4-cyclohexyl-1-butyl-beta-D-maltoside
Resolution 3.40 Å R-free 0.234
6Q8W Respiratory complex I from Thermus thermophilus with bound Aureothin. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain O 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQW Aureothin × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 25% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 7.6 mM 4-cyclohexyl-1-butyl-beta-D-maltoside
Resolution 3.40 Å R-free 0.234
6Q8X Respiratory complex I from Thermus thermophilus with bound Pyridaben. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 9 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQK Pyridaben × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 21% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 1.02 mM octyl-maltoside fluorinated
Resolution 3.51 Å R-free 0.232
6Q8X Respiratory complex I from Thermus thermophilus with bound Pyridaben. Deposited 2018-12-16 Different oligomeric state Different ligand/ion Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain O 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 HQK Pyridaben × 1 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；pH 6；296 K；100 mM Bis-Tris pH 6.0, 21% (w/v) PEG 4000, 100 mM KCl, 100 mM glutaric acid and 1.02 mM octyl-maltoside fluorinated
Resolution 3.51 Å R-free 0.232
6Y11 Respiratory complex I from Thermus thermophilus Deposited 2020-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain 9 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM
Resolution 3.11 Å R-free 0.229
6Y11 Respiratory complex I from Thermus thermophilus Deposited 2020-02-10 Different oligomeric state Different experimental conditions Different structure-quality metrics Assembly 2 Protein heterocomplex Heteromer；Protein × 16 PDB declaration: hexadecameric
Chain O 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 X-RAY DIFFRACTION
X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；295 K；100 mM Bis-Tris pH 6.0, 26% (w/v) polyethylene glycol (PEG) 4000, 100 mM KCl, 100 mM glutaric acid pH 6.0 and 0.6% DDM
Resolution 3.11 Å R-free 0.229
6ZIY Respiratory complex I from Thermus thermophilus, NADH dataset, major state Deposited 2020-06-26 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 9 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 NAI 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.25 Å
6ZJL Respiratory complex I from Thermus thermophilus, NAD+ dataset, major state Deposited 2020-06-29 Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 9 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FMN FLAVIN MONONUCLEOTIDE × 1 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 4.30 Å
6ZJN Respiratory complex I from Thermus thermophilus, NADH dataset, minor state Deposited 2020-06-29 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 9 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 6.10 Å
6ZJY Respiratory complex I from Thermus thermophilus, NAD+ dataset, minor state Deposited 2020-06-29 Different oligomeric state Different ligand/ion Different experimental method Different experimental conditions Different structure-quality metrics Assembly 1 Protein heterocomplex Heteromer；Protein × 15 PDB declaration: pentadecameric
Chain 9 1–182（182 aa）
Not recorded SF4 IRON/SULFUR CLUSTER × 7 FES FE2/S2 (INORGANIC) CLUSTER × 2 ELECTRON MICROSCOPY
cryo-EM buffer pH 6
cryo-EM vitrification conditions Cryogen ETHANE
Resolution 5.50 Å

16 other PDB entries and 29 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence
UniProt name NQO9_THET8
Isoform —
PDB entities 7
Chains and sequence ranges Author chain 9； PDBConstruct 1–182； UniProt 1–182 Author chain G； PDBConstruct 1–182； UniProt 1–182 Author chain P； PDBConstruct 1–182； UniProt 1–182 Author chain Y； PDBConstruct 1–182； UniProt 1–182

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

SAXS scattering curve SAXS Profile

SAXS profile for 2fug

P(r) Distance Distribution P(r) Distribution

P(r) distribution for 2fug
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2. Structure Basics 2. Structure Basics

Entry ID entry_id2fug
Deposition date deposition_date2006-01-26
Structure title titleCrystal structure of the hydrophilic domain of respiratory complex I from Thermus thermophilus
Keywords keywordsOXIDOREDUCTASE, ELECTRON TRANSPORT, RESPIRATORY CHAIN; OXIDOREDUCTASE
Experimental Method methodX-RAY DIFFRACTION
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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier83.55 Å
Radius of gyration Rg (electron density) rg_electron83.84 Å
Forward intensity I(0) i015343400000.00
Molecular weight molecular_weight1056700.0 kDa
Excluded volume excluded_volume1321400 ų
Envelope volume envelope_volume2008400 ų
Hydration-shell volume shell_volume197520 ų
Envelope diameter envelope_diameter292.0 Å
Shell Rg shell_rg81.95 Å
Envelope Rg envelope_rg81.41 Å
Shape Rg shape_rg83.89 Å
Total Rg total_rg83.63 Å
Total atoms total_atoms73916
Residues n_residues9332
Spherical-harmonic order n_harmonics20
q range q_range— – 0.5000 Å−1
Data points n_points101
Shell type shell_typedirectional
Solvent electron density solvent_density0.3340 e/ų
Shell contrast contrast_shell0.0300 e/ų
CRYSOL version crysol_version4.1.3
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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax288.0 Å
Rg (real space) rg_real87.50 Å
Rg uncertainty (real space) rg_real_error1.67 Å
I(0) (real space) i0_real1.5370e+10
I(0) uncertainty (real space) i0_real_error3.1730e+08
Rg (reciprocal space) rg_reciprocal82.91 Å
I(0) (reciprocal space) i0_reciprocal15310000000.0000
Solution quality estimate total_estimate0.8835
Solution quality rating solution_quality GOOD a GOOD solution
P(r) peaks n_peaks1
Primary peak position r_peak_primary90.9 Å
Skewness Skewness skewness0.433
Kurtosis Kurtosis kurtosis-0.266
Angular range angular_range— – 0.0950 Å−1
Current regularization parameter α current_alpha1.3140
Highest regularization parameter α highest_alpha408400000.0000
Real-space data points n_real_points20
GNOM version gnom_version4.1.3
Quality Criteria quality_criteria AN1: 0.000; Oscil: 0.933; Stabil: 0.859; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.132
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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (11)

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7. Fold Classification (SCOP + CATH) 92 domains

SCOP 2.08 (52 domains)

Domain ID domain_idd2fug11
Class classa — All alpha proteins
Fold Fold folda.29 — Bromodomain-like
Superfamily Superfamily superfamilya.29.12 — Nqo1C-terminal domain-like
Family Family familya.29.12.1 — Nqo1C-terminal domain-like
Domain ID domain_idd2fug12
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.142 — Nqo1 FMN-binding domain-like
Superfamily Superfamily superfamilyc.142.1 — Nqo1 FMN-binding domain-like
Family Family familyc.142.1.1 — Nqo1 FMN-binding domain-like
Domain ID domain_idd2fug13
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.15 — beta-Grasp (ubiquitin-like)
Superfamily Superfamily superfamilyd.15.13 — Nqo1 middle domain-like
Family Family familyd.15.13.1 — Nqo1 middle domain-like
Domain ID domain_idd2fug21
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.47 — Thioredoxin fold
Superfamily Superfamily superfamilyc.47.1 — Thioredoxin-like
Family Family familyc.47.1.21 — NQO2-like
Domain ID domain_idd2fug31
Class classb — All beta proteins
Fold Fold foldb.52 — Double psi beta-barrel
Superfamily Superfamily superfamilyb.52.2 — ADC-like
Family Family familyb.52.2.2 — Formate dehydrogenase/DMSO reductase, C-terminal domain
Domain ID domain_idd2fug32
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.81 — Formate dehydrogenase/DMSO reductase, domains 1-3
Superfamily Superfamily superfamilyc.81.1 — Formate dehydrogenase/DMSO reductase, domains 1-3
Family Family familyc.81.1.1 — Formate dehydrogenase/DMSO reductase, domains 1-3
Domain ID domain_idd2fug33
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.15 — beta-Grasp (ubiquitin-like)
Superfamily Superfamily superfamilyd.15.4 — 2Fe-2S ferredoxin-like
Family Family familyd.15.4.2 — 2Fe-2S ferredoxin domains from multidomain proteins
Domain ID domain_idd2fug34
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.58 — Ferredoxin-like
Superfamily Superfamily superfamilyd.58.1 — 4Fe-4S ferredoxins
Family Family familyd.58.1.5 — Ferredoxin domains from multidomain proteins
Domain ID domain_idd2fug41
Class classe — Multi-domain proteins (alpha and beta)
Fold Fold folde.18 — HydB/Nqo4-like
Superfamily Superfamily superfamilye.18.1 — HydB/Nqo4-like
Family Family familye.18.1.2 — Nqo4-like
Domain ID domain_idd2fug51
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.307 — Nqo5-like
Superfamily Superfamily superfamilyd.307.1 — Nqo5-like
Family Family familyd.307.1.1 — Nqo5-like
Domain ID domain_idd2fug61
Class classe — Multi-domain proteins (alpha and beta)
Fold Fold folde.19 — HydA/Nqo6-like
Superfamily Superfamily superfamilye.19.1 — HydA/Nqo6-like
Family Family familye.19.1.2 — Nq06-like
Domain ID domain_idd2fug71
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.82 — N domain of copper amine oxidase-like
Superfamily Superfamily superfamilyd.82.2 — Frataxin/Nqo15-like
Family Family familyd.82.2.2 — Nqo15-like
Domain ID domain_idd2fug91
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.58 — Ferredoxin-like
Superfamily Superfamily superfamilyd.58.1 — 4Fe-4S ferredoxins
Family Family familyd.58.1.5 — Ferredoxin domains from multidomain proteins
Domain ID domain_idd2fuga1
Class classa — All alpha proteins
Fold Fold folda.29 — Bromodomain-like
Superfamily Superfamily superfamilya.29.12 — Nqo1C-terminal domain-like
Family Family familya.29.12.1 — Nqo1C-terminal domain-like
Domain ID domain_idd2fuga2
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.142 — Nqo1 FMN-binding domain-like
Superfamily Superfamily superfamilyc.142.1 — Nqo1 FMN-binding domain-like
Family Family familyc.142.1.1 — Nqo1 FMN-binding domain-like
Domain ID domain_idd2fuga3
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.15 — beta-Grasp (ubiquitin-like)
Superfamily Superfamily superfamilyd.15.13 — Nqo1 middle domain-like
Family Family familyd.15.13.1 — Nqo1 middle domain-like
Domain ID domain_idd2fugb1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.47 — Thioredoxin fold
Superfamily Superfamily superfamilyc.47.1 — Thioredoxin-like
Family Family familyc.47.1.21 — NQO2-like
Domain ID domain_idd2fugc1
Class classb — All beta proteins
Fold Fold foldb.52 — Double psi beta-barrel
Superfamily Superfamily superfamilyb.52.2 — ADC-like
Family Family familyb.52.2.2 — Formate dehydrogenase/DMSO reductase, C-terminal domain
Domain ID domain_idd2fugc2
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.81 — Formate dehydrogenase/DMSO reductase, domains 1-3
Superfamily Superfamily superfamilyc.81.1 — Formate dehydrogenase/DMSO reductase, domains 1-3
Family Family familyc.81.1.1 — Formate dehydrogenase/DMSO reductase, domains 1-3
Domain ID domain_idd2fugc3
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.15 — beta-Grasp (ubiquitin-like)
Superfamily Superfamily superfamilyd.15.4 — 2Fe-2S ferredoxin-like
Family Family familyd.15.4.2 — 2Fe-2S ferredoxin domains from multidomain proteins
Domain ID domain_idd2fugc4
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.58 — Ferredoxin-like
Superfamily Superfamily superfamilyd.58.1 — 4Fe-4S ferredoxins
Family Family familyd.58.1.5 — Ferredoxin domains from multidomain proteins
Domain ID domain_idd2fugd1
Class classe — Multi-domain proteins (alpha and beta)
Fold Fold folde.18 — HydB/Nqo4-like
Superfamily Superfamily superfamilye.18.1 — HydB/Nqo4-like
Family Family familye.18.1.2 — Nqo4-like
Domain ID domain_idd2fuge1
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.307 — Nqo5-like
Superfamily Superfamily superfamilyd.307.1 — Nqo5-like
Family Family familyd.307.1.1 — Nqo5-like
Domain ID domain_idd2fugf1
Class classe — Multi-domain proteins (alpha and beta)
Fold Fold folde.19 — HydA/Nqo6-like
Superfamily Superfamily superfamilye.19.1 — HydA/Nqo6-like
Family Family familye.19.1.2 — Nq06-like
Domain ID domain_idd2fugg1
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.58 — Ferredoxin-like
Superfamily Superfamily superfamilyd.58.1 — 4Fe-4S ferredoxins
Family Family familyd.58.1.5 — Ferredoxin domains from multidomain proteins
Domain ID domain_idd2fugh1
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.82 — N domain of copper amine oxidase-like
Superfamily Superfamily superfamilyd.82.2 — Frataxin/Nqo15-like
Family Family familyd.82.2.2 — Nqo15-like
Domain ID domain_idd2fugj1
Class classa — All alpha proteins
Fold Fold folda.29 — Bromodomain-like
Superfamily Superfamily superfamilya.29.12 — Nqo1C-terminal domain-like
Family Family familya.29.12.1 — Nqo1C-terminal domain-like
Domain ID domain_idd2fugj2
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.142 — Nqo1 FMN-binding domain-like
Superfamily Superfamily superfamilyc.142.1 — Nqo1 FMN-binding domain-like
Family Family familyc.142.1.1 — Nqo1 FMN-binding domain-like
Domain ID domain_idd2fugj3
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.15 — beta-Grasp (ubiquitin-like)
Superfamily Superfamily superfamilyd.15.13 — Nqo1 middle domain-like
Family Family familyd.15.13.1 — Nqo1 middle domain-like
Domain ID domain_idd2fugk1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.47 — Thioredoxin fold
Superfamily Superfamily superfamilyc.47.1 — Thioredoxin-like
Family Family familyc.47.1.21 — NQO2-like
Domain ID domain_idd2fugl1
Class classb — All beta proteins
Fold Fold foldb.52 — Double psi beta-barrel
Superfamily Superfamily superfamilyb.52.2 — ADC-like
Family Family familyb.52.2.2 — Formate dehydrogenase/DMSO reductase, C-terminal domain
Domain ID domain_idd2fugl2
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.81 — Formate dehydrogenase/DMSO reductase, domains 1-3
Superfamily Superfamily superfamilyc.81.1 — Formate dehydrogenase/DMSO reductase, domains 1-3
Family Family familyc.81.1.1 — Formate dehydrogenase/DMSO reductase, domains 1-3
Domain ID domain_idd2fugl3
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.15 — beta-Grasp (ubiquitin-like)
Superfamily Superfamily superfamilyd.15.4 — 2Fe-2S ferredoxin-like
Family Family familyd.15.4.2 — 2Fe-2S ferredoxin domains from multidomain proteins
Domain ID domain_idd2fugl4
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.58 — Ferredoxin-like
Superfamily Superfamily superfamilyd.58.1 — 4Fe-4S ferredoxins
Family Family familyd.58.1.5 — Ferredoxin domains from multidomain proteins
Domain ID domain_idd2fugm1
Class classe — Multi-domain proteins (alpha and beta)
Fold Fold folde.18 — HydB/Nqo4-like
Superfamily Superfamily superfamilye.18.1 — HydB/Nqo4-like
Family Family familye.18.1.2 — Nqo4-like
Domain ID domain_idd2fugn1
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.307 — Nqo5-like
Superfamily Superfamily superfamilyd.307.1 — Nqo5-like
Family Family familyd.307.1.1 — Nqo5-like
Domain ID domain_idd2fugo1
Class classe — Multi-domain proteins (alpha and beta)
Fold Fold folde.19 — HydA/Nqo6-like
Superfamily Superfamily superfamilye.19.1 — HydA/Nqo6-like
Family Family familye.19.1.2 — Nq06-like
Domain ID domain_idd2fugp1
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.58 — Ferredoxin-like
Superfamily Superfamily superfamilyd.58.1 — 4Fe-4S ferredoxins
Family Family familyd.58.1.5 — Ferredoxin domains from multidomain proteins
Domain ID domain_idd2fugq1
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.82 — N domain of copper amine oxidase-like
Superfamily Superfamily superfamilyd.82.2 — Frataxin/Nqo15-like
Family Family familyd.82.2.2 — Nqo15-like
Domain ID domain_idd2fugs1
Class classa — All alpha proteins
Fold Fold folda.29 — Bromodomain-like
Superfamily Superfamily superfamilya.29.12 — Nqo1C-terminal domain-like
Family Family familya.29.12.1 — Nqo1C-terminal domain-like
Domain ID domain_idd2fugs2
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.142 — Nqo1 FMN-binding domain-like
Superfamily Superfamily superfamilyc.142.1 — Nqo1 FMN-binding domain-like
Family Family familyc.142.1.1 — Nqo1 FMN-binding domain-like
Domain ID domain_idd2fugs3
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.15 — beta-Grasp (ubiquitin-like)
Superfamily Superfamily superfamilyd.15.13 — Nqo1 middle domain-like
Family Family familyd.15.13.1 — Nqo1 middle domain-like
Domain ID domain_idd2fugt1
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.47 — Thioredoxin fold
Superfamily Superfamily superfamilyc.47.1 — Thioredoxin-like
Family Family familyc.47.1.21 — NQO2-like
Domain ID domain_idd2fugu1
Class classb — All beta proteins
Fold Fold foldb.52 — Double psi beta-barrel
Superfamily Superfamily superfamilyb.52.2 — ADC-like
Family Family familyb.52.2.2 — Formate dehydrogenase/DMSO reductase, C-terminal domain
Domain ID domain_idd2fugu2
Class classc — Alpha and beta proteins (a/b)
Fold Fold foldc.81 — Formate dehydrogenase/DMSO reductase, domains 1-3
Superfamily Superfamily superfamilyc.81.1 — Formate dehydrogenase/DMSO reductase, domains 1-3
Family Family familyc.81.1.1 — Formate dehydrogenase/DMSO reductase, domains 1-3
Domain ID domain_idd2fugu3
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.15 — beta-Grasp (ubiquitin-like)
Superfamily Superfamily superfamilyd.15.4 — 2Fe-2S ferredoxin-like
Family Family familyd.15.4.2 — 2Fe-2S ferredoxin domains from multidomain proteins
Domain ID domain_idd2fugu4
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.58 — Ferredoxin-like
Superfamily Superfamily superfamilyd.58.1 — 4Fe-4S ferredoxins
Family Family familyd.58.1.5 — Ferredoxin domains from multidomain proteins
Domain ID domain_idd2fugv1
Class classe — Multi-domain proteins (alpha and beta)
Fold Fold folde.18 — HydB/Nqo4-like
Superfamily Superfamily superfamilye.18.1 — HydB/Nqo4-like
Family Family familye.18.1.2 — Nqo4-like
Domain ID domain_idd2fugw1
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.307 — Nqo5-like
Superfamily Superfamily superfamilyd.307.1 — Nqo5-like
Family Family familyd.307.1.1 — Nqo5-like
Domain ID domain_idd2fugx1
Class classe — Multi-domain proteins (alpha and beta)
Fold Fold folde.19 — HydA/Nqo6-like
Superfamily Superfamily superfamilye.19.1 — HydA/Nqo6-like
Family Family familye.19.1.2 — Nq06-like
Domain ID domain_idd2fugy1
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.58 — Ferredoxin-like
Superfamily Superfamily superfamilyd.58.1 — 4Fe-4S ferredoxins
Family Family familyd.58.1.5 — Ferredoxin domains from multidomain proteins
Domain ID domain_idd2fugz1
Class classd — Alpha and beta proteins (a+b)
Fold Fold foldd.82 — N domain of copper amine oxidase-like
Superfamily Superfamily superfamilyd.82.2 — Frataxin/Nqo15-like
Family Family familyd.82.2.2 — Nqo15-like

CATH v4.4 (40 domains)

Domain ID domain_id2fug101
Class class6 — Special
Architecture architecture10 — Helix non-globular
Topology topology250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily1450 —
Domain ID domain_id2fug102
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily11540 — NADH-ubiquinone oxidoreductase 51kDa subunit
Domain ID domain_id2fug103
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily600 —
Domain ID domain_id2fug104
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1440 — de novo design (two linked rop proteins)
Homologous superfamily homologous superfamily230 — NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain
Domain ID domain_id2fug201
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology10 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily1590 — NADH-quinone oxidoreductase subunit E
Domain ID domain_id2fug202
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology30 — Glutaredoxin
Homologous superfamily homologous superfamily10 — Glutaredoxin
Domain ID domain_id2fug400
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology645 — Cytochrome-c3 Hydrogenase; chain B
Homologous superfamily homologous superfamily10 — Cytochrome-c3 Hydrogenase, chain B
Domain ID domain_id2fug600
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily12280 —
Domain ID domain_id2fug700
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology920 — Metal Transport, Frataxin; Chain A
Homologous superfamily homologous superfamily80 — NADH-quinone oxidoreductase, subunit 15
Domain ID domain_id2fug900
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily3270 —
Domain ID domain_id2fugA01
Class class6 — Special
Architecture architecture10 — Helix non-globular
Topology topology250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily1450 —
Domain ID domain_id2fugA02
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily11540 — NADH-ubiquinone oxidoreductase 51kDa subunit
Domain ID domain_id2fugA03
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily600 —
Domain ID domain_id2fugA04
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1440 — de novo design (two linked rop proteins)
Homologous superfamily homologous superfamily230 — NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain
Domain ID domain_id2fugB01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology10 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily1590 — NADH-quinone oxidoreductase subunit E
Domain ID domain_id2fugB02
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology30 — Glutaredoxin
Homologous superfamily homologous superfamily10 — Glutaredoxin
Domain ID domain_id2fugD00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology645 — Cytochrome-c3 Hydrogenase; chain B
Homologous superfamily homologous superfamily10 — Cytochrome-c3 Hydrogenase, chain B
Domain ID domain_id2fugF00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily12280 —
Domain ID domain_id2fugG00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily3270 —
Domain ID domain_id2fugH00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology920 — Metal Transport, Frataxin; Chain A
Homologous superfamily homologous superfamily80 — NADH-quinone oxidoreductase, subunit 15
Domain ID domain_id2fugJ01
Class class6 — Special
Architecture architecture10 — Helix non-globular
Topology topology250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily1450 —
Domain ID domain_id2fugJ02
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily11540 — NADH-ubiquinone oxidoreductase 51kDa subunit
Domain ID domain_id2fugJ03
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily600 —
Domain ID domain_id2fugJ04
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1440 — de novo design (two linked rop proteins)
Homologous superfamily homologous superfamily230 — NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain
Domain ID domain_id2fugK01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology10 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily1590 — NADH-quinone oxidoreductase subunit E
Domain ID domain_id2fugK02
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology30 — Glutaredoxin
Homologous superfamily homologous superfamily10 — Glutaredoxin
Domain ID domain_id2fugM00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology645 — Cytochrome-c3 Hydrogenase; chain B
Homologous superfamily homologous superfamily10 — Cytochrome-c3 Hydrogenase, chain B
Domain ID domain_id2fugO00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily12280 —
Domain ID domain_id2fugP00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily3270 —
Domain ID domain_id2fugQ00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology920 — Metal Transport, Frataxin; Chain A
Homologous superfamily homologous superfamily80 — NADH-quinone oxidoreductase, subunit 15
Domain ID domain_id2fugS01
Class class6 — Special
Architecture architecture10 — Helix non-globular
Topology topology250 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Homologous superfamily homologous superfamily1450 —
Domain ID domain_id2fugS02
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily11540 — NADH-ubiquinone oxidoreductase 51kDa subunit
Domain ID domain_id2fugS03
Class class3 — Alpha Beta
Architecture architecture10 — Roll
Topology topology20 — Ubiquitin-like (UB roll)
Homologous superfamily homologous superfamily600 —
Domain ID domain_id2fugS04
Class class1 — Mainly Alpha
Architecture architecture20 — Up-down Bundle
Topology topology1440 — de novo design (two linked rop proteins)
Homologous superfamily homologous superfamily230 — NADH-ubiquinone oxidoreductase 51kDa subunit, iron-sulphur binding domain
Domain ID domain_id2fugT01
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology10 — Arc Repressor Mutant, subunit A
Homologous superfamily homologous superfamily1590 — NADH-quinone oxidoreductase subunit E
Domain ID domain_id2fugT02
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology30 — Glutaredoxin
Homologous superfamily homologous superfamily10 — Glutaredoxin
Domain ID domain_id2fugV00
Class class1 — Mainly Alpha
Architecture architecture10 — Orthogonal Bundle
Topology topology645 — Cytochrome-c3 Hydrogenase; chain B
Homologous superfamily homologous superfamily10 — Cytochrome-c3 Hydrogenase, chain B
Domain ID domain_id2fugX00
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily12280 —
Domain ID domain_id2fugY00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology70 — Alpha-Beta Plaits
Homologous superfamily homologous superfamily3270 —
Domain ID domain_id2fugZ00
Class class3 — Alpha Beta
Architecture architecture30 — 2-Layer Sandwich
Topology topology920 — Metal Transport, Frataxin; Chain A
Homologous superfamily homologous superfamily80 — NADH-quinone oxidoreductase, subunit 15
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8. Citations (2)

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9. Files and Curves (10)