SAXSdb — 2hrj

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1. Protein Identity and Related Structures Protein Identity & Related Structures

Talin-1

Gallus gallus

State in the Current Structure

Assembly	Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Associated Components	Method and Experimental Conditions	Structure Quality
1	Protein monomer Monomer Protein × 1 PDB declaration: monomeric（1） Consistent with protein copy count	Chain A； UniProt 189–309	Fragment：F2 subdomain	No other associated polymer	SOLUTION NMR NMR measurement conditions：pH 6.5；298 K；Pressure ambient NMR measurement conditions：pH 6.5；310 K；Pressure ambient	Resolution not provided

Other States of the Same Protein in the Database

Each row is a biological assembly of the same UniProt protein in another PDB entry. The “Difference from current entry” column identifies evidence-level differences; no tag means the currently parsed fields agree.

Other PDB	Difference from Current Entry 2HRJ	Assembly / Oligomeric State	Construct	Mutations and Modifications	Ligands, Ions and Non-polymers	Method and Experimental Conditions	Structure Quality
1MIX Crystal structure of a FERM domain of Talin Deposited 2002-08-23	Different construct Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein monomer Monomer；Protein × 1 PDB declaration: monomeric	Chain A 196–400（205 aa） Fragment：Second and third subdomains of FERM domain (Residues 196-400)	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；VAPOR DIFFUSION, HANGING DROP	Resolution 1.75 Å R-free 0.239
1MIZ Crystal structure of an integrin beta3-talin chimera Deposited 2002-08-23	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 200–400（201 aa） Fragment：Residues 200-400	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；pH 7.0, VAPOR DIFFUSION, HANGING DROP	Resolution 1.90 Å R-free 0.246
1MK7 CRYSTAL STRUCTURE OF AN INTEGRIN BETA3-TALIN CHIMERA Deposited 2002-08-28	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain D 209–400（192 aa） Fragment：F2 AND F3 SUBDOMAINS OF FERM DOMAIN (Residues 209-400)	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；0.1 M TRIS-HCL, 18% (W/V) PEG-MME 2000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.20 Å R-free 0.250
1MK7 CRYSTAL STRUCTURE OF AN INTEGRIN BETA3-TALIN CHIMERA Deposited 2002-08-28	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 209–400（192 aa） Fragment：F2 AND F3 SUBDOMAINS OF FERM DOMAIN (Residues 209-400)	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 7；277 K；0.1 M TRIS-HCL, 18% (W/V) PEG-MME 2000, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.20 Å R-free 0.250
1MK9 CRYSTAL STRUCTURE OF AN INTEGRIN BETA3-TALIN CHIMERA Deposited 2002-08-28	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain D 209–400（192 aa） Fragment：F2 AND F3 SUBDOMAINS OF FERM DOMAIN (Residues 209-400)	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.6；277 K；PEG 8000, MAGNESIUM CHLORIDE, TRIS-HCL, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.80 Å R-free 0.327
1MK9 CRYSTAL STRUCTURE OF AN INTEGRIN BETA3-TALIN CHIMERA Deposited 2002-08-28	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 2 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 209–400（192 aa） Fragment：F2 AND F3 SUBDOMAINS OF FERM DOMAIN (Residues 209-400)	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.6；277 K；PEG 8000, MAGNESIUM CHLORIDE, TRIS-HCL, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.80 Å R-free 0.327
1MK9 CRYSTAL STRUCTURE OF AN INTEGRIN BETA3-TALIN CHIMERA Deposited 2002-08-28	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 3 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain H 209–400（192 aa） Fragment：F2 AND F3 SUBDOMAINS OF FERM DOMAIN (Residues 209-400)	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.6；277 K；PEG 8000, MAGNESIUM CHLORIDE, TRIS-HCL, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.80 Å R-free 0.327
1MK9 CRYSTAL STRUCTURE OF AN INTEGRIN BETA3-TALIN CHIMERA Deposited 2002-08-28	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 4 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain F 209–400（192 aa） Fragment：F2 AND F3 SUBDOMAINS OF FERM DOMAIN (Residues 209-400)	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.6；277 K；PEG 8000, MAGNESIUM CHLORIDE, TRIS-HCL, pH 8.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K	Resolution 2.80 Å R-free 0.327
1RKC Human vinculin head (1-258) in complex with talin's vinculin binding site 3 (residues 1944-1969) Deposited 2003-11-21	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 1944–1969（26 aa） Fragment：binding site 3 (residues 1944-1969)	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions pH 4；293 K；2% MPD; 100 mM citric acid (pH 4); 100 mM CdCl2, pH 4.0, temperature 293K	Resolution 2.70 Å R-free 0.335
1ZVZ Vinculin Head (0-258) in Complex with the Talin Rod Residue 820-844 Deposited 2005-06-03	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 820–844（25 aa） Fragment：Residues 820-844	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, SITTING DROP；293 K；VAPOR DIFFUSION, SITTING DROP, temperature 293K	Resolution 1.80 Å R-free 0.276
2H7D Solution structure of the talin F3 domain in complex with a chimeric beta3 integrin-PIP kinase peptide Deposited 2006-06-02	Different construct Different mutation/modification Different oligomeric state Different experimental conditions	Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 309–405（97 aa） Fragment：F3 domain	Mutation：C336S	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 6.1；298 K；Ionic strength (raw mmCIF value) 100mM NaCl；Pressure ambient NMR sample composition 1.2mM Talin F3 U-15N; 1.4mM chimeric peptide; 50mM phosphate buffer pH 6.1, 100mM NaCl; 95% H2O, 5% D2O \| 95% H2O/5% D2O NMR sample composition 1.5mM Talin F3; 1.5mM chimeric peptide; 50mM phosphate buffer pH 6.1, 100mM NaCl; 99.96% D2O \| 99.96% D2O NMR sample composition 1.9mM Talin F3 U-15N,2H; 1.5mM chimeric peptide; 50mM phosphate buffer pH 6.1, 100mM NaCl; 95% H2O, 5% D2O \| 95% H2O/5% D2O NMR sample composition 1.9mM Talin F3 U-15N,2H; 1.5mM chimeric peptide; 50mM phosphate buffer pH 6.1, 100mM NaCl; 99.96% D2O \| 99.96% D2O NMR sample composition 0.9mM Talin F3 U-15N,13C; 1.1mM chimeric peptide; 50mM phosphate buffer pH 6.1, 100mM NaCl; 95% H2O, 5% D2O \| 95% H2O/5% D2O	Resolution not provided
2H7E Solution structure of the talin F3 domain in complex with a chimeric beta3 integrin-PIP kinase peptide- minimized average structure Deposited 2006-06-02	Different construct Different mutation/modification Different oligomeric state Different experimental conditions	Assembly 1 Insufficient information Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 309–405（97 aa） Fragment：F3 domain	Mutation：C336S	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 6.1；298 K；Ionic strength (raw mmCIF value) 100mM NaCl；Pressure ambient NMR sample composition 1.2mM Talin F3 U-15N; 1.4mM chimeric peptide; 50mM phosphate buffer pH 6.1, 100mM NaCl; 95% H2O, 5% D2O \| 95% H2O/5% D2O NMR sample composition 1.5mM Talin F3; 1.5mM chimeric peptide; 50mM phosphate buffer pH 6.1, 100mM NaCl; 99.96% D2O \| 99.96% D2O NMR sample composition 1.9mM Talin F3 U-15N,2H; 1.5mM chimeric peptide; 50mM phosphate buffer pH 6.1, 100mM NaCl; 95% H2O, 5% D2O \| 95% H2O/5% D2O NMR sample composition 1.9mM Talin F3 U-15N,2H; 1.5mM chimeric peptide; 50mM phosphate buffer pH 6.1, 100mM NaCl; 99.96% D2O \| 99.96% D2O NMR sample composition 0.9mM Talin F3 U-15N,13C; 1.1mM chimeric peptide; 50mM phosphate buffer pH 6.1, 100mM NaCl; 95% H2O, 5% D2O \| 95% H2O/5% D2O	Resolution not provided
2K00 Solution structure of the talin F3 in complex with layilin cytodomain Deposited 2008-01-23	Different construct Different oligomeric state Different experimental conditions	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain A 309–400（92 aa） Fragment：residues 309-400	Not recorded	No recorded non-water small molecule	SOLUTION NMR NMR measurement conditions pH 6.1；298 K；Ionic strength (raw mmCIF value) 150；Pressure ambient NMR sample composition 1 mM [U-13C; U-15N] protein, 3.5 mM peptide, 100 mM sodium chloride, 50 mM sodium phosphate, 90% H2O/10% D2O \| 90% H2O/10% D2O	Resolution not provided
8S4Y Crystal structure of an APP-talin (F2F3) chimera Deposited 2024-02-22	Different construct Different oligomeric state Different experimental method Different experimental conditions Different structure-quality metrics	Assembly 1 Protein heterocomplex Heteromer；Protein × 2 PDB declaration: dimeric	Chain B 209–400（192 aa）	Not recorded	No recorded non-water small molecule	X-RAY DIFFRACTION X-ray crystallization conditions VAPOR DIFFUSION, HANGING DROP；pH 8.5；291 K；0.1 M Tris pH 8.5, 10% v/v ethanol	Resolution 2.70 Å R-free 0.267

10 other PDB entries and 14 assemblies. Open the comparison page and filter oligomeric states

View Construct and Data Evidence

UniProt name	TLN1_CHICK
Isoform	—
PDB entities	1
Chains and sequence ranges	Author chain A； PDBConstruct 1–121； UniProt 189–309

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id	2hrj
Deposition date deposition_date	2006-07-20
Structure title title	NMR solution structure of the F2 subdomain of talin
Keywords keywords	ACBP-like, talin, STRUCTURAL PROTEIN; STRUCTURAL PROTEIN
Experimental Method method	SOLUTION NMR

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3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

Radius of gyration Rg (Guinier) rg_guinier	15.90 Å
Radius of gyration Rg (electron density) rg_electron	16.12 Å
Forward intensity I(0) i0	1710020000.00
Molecular weight molecular_weight	353630.0 kDa
Excluded volume excluded_volume	443980 Å³
Envelope volume envelope_volume	78696 Å³
Hydration-shell volume shell_volume	26978 Å³
Envelope diameter envelope_diameter	93.7 Å
Shell Rg shell_rg	31.65 Å
Envelope Rg envelope_rg	26.45 Å
Shape Rg shape_rg	16.08 Å
Total Rg total_rg	16.59 Å
Total atoms total_atoms	49775
Residues n_residues	3025
Spherical-harmonic order n_harmonics	20
q range q_range	— – 0.5000 Å⁻¹
Data points n_points	101
Shell type shell_type	directional
Solvent electron density solvent_density	0.3340 e/Å³
Shell contrast contrast_shell	0.0300 e/Å³
CRYSOL version crysol_version	4.1.3

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4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

Maximum dimension Dmax dmax	40.1 Å
Rg (real space) rg_real	14.65 Å
Rg uncertainty (real space) rg_real_error	0.05 Å
I(0) (real space) i0_real	1.6130e+09
I(0) uncertainty (real space) i0_real_error	1.3770e+07
Rg (reciprocal space) rg_reciprocal	16.07 Å
I(0) (reciprocal space) i0_reciprocal	1710000000.0000
Solution quality estimate total_estimate	0.6876
Solution quality rating solution_quality	REASONABLE a REASONABLE solution
P(r) peaks n_peaks	1
Primary peak position r_peak_primary	16.8 Å
Skewness Skewness skewness	0.193
Kurtosis Kurtosis kurtosis	-0.451
Angular range angular_range	— – 0.5000 Å⁻¹
Current regularization parameter α current_alpha	3.1450
Highest regularization parameter α highest_alpha	604300.0000
Real-space data points n_real_points	80
GNOM version gnom_version	4.1.3
Quality Criteria quality_criteria	AN1: 0.033; Oscil: 0.994; Stabil: 0.988; Sysdev: 0.000; Positv: 1.000; Valcen: 0.994; Smooth: 0.000

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5. Crystallography and Experiment 5. Crystallography & Experiment

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6. Entities and Polymers Entities & Polymers (1)

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7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

Domain ID domain_id	d2hrja_
Class class	a — All alpha proteins
Fold Fold fold	a.11 — Acyl-CoA binding protein-like
Superfamily Superfamily superfamily	a.11.2 — Second domain of FERM
Family Family family	a.11.2.1 — Second domain of FERM

CATH v4.4 (1 domains)

Domain ID domain_id	2hrjA01
Class class	1 — Mainly Alpha
Architecture architecture	20 — Up-down Bundle
Topology topology	80 — Acyl-CoA Binding Protein
Homologous superfamily homologous superfamily	10 —

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8. Citations (1)

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Unit-cell length a length_a	1.000 Å
Unit-cell length b length_b	1.000 Å
Unit-cell length c length_c	1.000 Å
Unit-cell angle α angle_alpha	90.00 °
Unit-cell angle β angle_beta	90.00 °
Unit-cell angle γ angle_gamma	90.00 °
Molecules per unit cell Z z_pdb	1

Space group space_group	P 1
Space-group number int_tables_number	1
Crystal system crystal_system	—

Description description	Talin-1
Molecular weight formula_weight	14181.3 kDa
Source method src_method	man
Copy count entity_count	1

Polymer type type	polypeptide(L)
Chain IDs strand_id	A
Atom count atom_count	49775

来源生物 organism	Gallus gallus
通用名 common_name	chicken
NCBI Class编号 ncbi_taxonomy_id	9031
宿主生物 host_organism	Escherichia coli

NMR solution structure of the F2 subdomain of talin

1. Protein Identity and Related Structures Protein Identity & Related Structures

Talin-1

State in the Current Structure

Other States of the Same Protein in the Database

SAXS scattering curve SAXS Profile

P(r) Distance Distribution P(r) Distribution

2. Structure Basics 2. Structure Basics

3. SAXS Parameters (CRYSOL theoretical calculation) 3. SAXS Parameters (CRYSOL)

4. P(r) Distance Distribution (GNOM inversion) 4. P(r) Analysis (GNOM)

5. Crystallography and Experiment 5. Crystallography & Experiment

Unit cell Unit Cell

Symmetry Symmetry

6. Entities and Polymers Entities & Polymers (1)

Entity 1 Entity 1 polymer

Polymer Polymer

来源 Source

7. Fold Classification (SCOP + CATH) 2 domains

SCOP 2.08 (1 domains)

CATH v4.4 (1 domains)

8. Citations (1)

9. Files and Curves (10)

Files (7)

Curves (3)

fit	/app/data/saxs_computation/hr/2hrj/2hrj.fit
pddf	/app/data/pr_computation/hr/2hrj/2hrj.pr.out
profile	/app/data/saxs_computation/hr/2hrj/2hrj.dat