2j04

The tau60-tau91 subcomplex of yeast transcription factor IIIC

Method: X-RAY DIFFRACTION D_max: 217.1 Å Quality: REASONABLE

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

下载 Download SAXS (.dat) P(r) (.pr.out) CIF

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1. 结构基本信息 1. Structure Basics

条目编号 entry_id	2j04
沉积日期 deposition_date	2006-07-31
结构标题 title	The tau60-tau91 subcomplex of yeast transcription factor IIIC
关键词 keywords	;BETA PROPELLER, TYPE 2 PROMOTERS, TRANSCRIPTION, HYPOTHETICAL PROTEIN, PREINITIATION COMPLEX, YEAST RNA POLYMERASE III, TRANSCRIPTION FACTOR IIIC ;; TRANSCRIPTION
实验方法 method	X-RAY DIFFRACTION

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2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	62.44 Å
回转半径 Rg (电子) rg_electron	64.87 Å
零角强度 I(0) i0	781375000.00
分子量 molecular_weight	240120.0 kDa
排除体积 excluded_volume	303180 Å³
包络体积 envelope_volume	423720 Å³
水化壳体积 shell_volume	63957 Å³
包络直径 envelope_diameter	245.1 Å
壳层 Rg shell_rg	49.65 Å
包络 Rg envelope_rg	65.85 Å
形状 Rg shape_rg	64.84 Å
总 Rg total_rg	64.55 Å
总原子数 total_atoms	16930
残基数 n_residues	2114
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

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3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	217.1 Å
Rg (实空间) rg_real	63.93 Å
Rg 误差 (实空间) rg_real_error	2.37 Å
I(0) (实空间) i0_real	7.8050e+08
I(0) 误差 (实空间) i0_real_error	1.6830e+07
Rg (倒空间) rg_reciprocal	60.92 Å
I(0) (倒空间) i0_reciprocal	777100000.0000
解质量估计 total_estimate	0.6527
解质量评级 solution_quality	REASONABLE a REASONABLE solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	34.7 Å
偏度 Skewness skewness	0.697
峰度 Kurtosis kurtosis	-0.315
角度范围 angular_range	— – 0.1250 Å⁻¹
当前正则化参数 α current_alpha	0.0029
最高正则化参数 α highest_alpha	39340000.0000
实空间数据点数 n_real_points	26
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.274; Stabil: 0.996; Sysdev: 1.000; Positv: 1.000; Valcen: 0.366; Smooth: 0.303

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4. 晶体学与实验 4. Crystallography & Experiment

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5. 实体与聚合体信息 (3)

Entity 1 polymer

描述 description	HYPOTHETICAL PROTEIN YPL007C
分子量 formula_weight	67755.2 kDa
来源方法 src_method	man
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A,C
原子数 atom_count	16935

MKLLKDLLVDRKEFEDWKNNLTWARDGTLYLTTFPDISIGQPKYAKDINCNSKNLFHVKEFPLEFENKLDFELAQQNGLLNSQPVCYPRVCKPSPIDDWMAVLSNNGNVSVFKDNKMLTNLDSKGNLSSRTYHCFEWNPIESSIVVGNEDGELQFFSIRKNSENTPEFYFESSIRLSDAGSKDWVTHIVWYEDVLVAALSNNSVFSMTVSASSHQPVSRMIQNASRRKITDLKIVDYKVVLTCPGYVHKIDLKNYSISSLKTGSLENFHIIPLNHEKESTILLMSNKTSYKVLLEDELHVTADNIIAPYLEKKFKKWSTIWNEFNNYETTLVIHGISLSPDGYSIAIVYDMERVAFKYKIASEQSFNIMFAPLYHTWTISERAVGLAWYQTYQIYNQSLPKLPENFSMNKKLLNGNYPISLDFQSYLNALMKSEEMRIIMFLNMTIDKPSILSFLEALYEYAINKKSELTNSFDLACVLSIAAILKREAPIYNGTLLMKNSFLEETFNLESFTADPETVTSTTNNTWKRCGVTLLPILTTHVKICPVSKQRVIDIKRDDLNDYGWFTRGLLERFNEISVYCGTTLEVM

来源 Source

来源生物 organism	SACCHAROMYCES CEREVISIAE
通用名 common_name	;BAKER'S YEAST ;
NCBI 分类编号 ncbi_taxonomy_id	4932
宿主生物 host_organism	SPODOPTERA FRUGIPERDA

Entity 2 polymer

描述 description	YDR362CP
分子量 formula_weight	58928.9 kDa
来源方法 src_method	man
拷贝数 entity_count	2

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	B,D

MGLLKDLSSARDKIERIYGLNKEKLLLLAKVKEGFETSVFDFPFKNIQPDSPYFVCLDPPCKKESAYNKVIGDKNRTVYHEINKTEFENMIKLRTKRLKLLIGEVDAEVSTGDKIEFPVLANGKRRGFIYNVGGLVTDIAWLNIEENTDIGKDIQYLAVAVSQYMDEPLNEHLEMFDKEKHSSCIQIFKMNTSTLHCVKVQTIVHSFGEVWDLKWHEGCHAPHLVGCLSFVSQEGTINFLEIIDNATDVHVFKMCEKPSLTLSLADSLITTFDFLSPTTVVCGFKNGFVAEFDLTDPEVPSFYDQVHDSYILSVSTAYSDFEDTVVSTVAVDGYFYIFNPKDIATTKTTVSRFRGSNLVPVVYCPQIYSYIYSDGASSLRAVPSRAAFAVHPLVSRETTITAIGVSRLHPMVLAGSADGSLIITNAARRLLHGIKNSSATQKSLRLWKWDYSIKDDKYRIDSSYEVYPLTVNDVSKAKIDAHGINITCTKWNETSAGGKCYAFSNSAGLLTLEYLSLEHHHHHH

来源 Source

来源生物 organism	SACCHAROMYCES CEREVISIAE
通用名 common_name	;BAKER'S YEAST ;
NCBI 分类编号 ncbi_taxonomy_id	4932
宿主生物 host_organism	ESCHERICHIA COLI BL21(DE3)

Entity 3 water

描述 description	water
分子量 formula_weight	18.0 kDa
来源方法 src_method	nat
拷贝数 entity_count	5

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7. 引用文献 (1)

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晶胞边长 a length_a	61.420 Å
晶胞边长 b length_b	125.800 Å
晶胞边长 c length_c	210.470 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	94.49 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	4

空间群 space_group	P 1 21 1
空间群编号 int_tables_number	4
晶系 crystal_system	—

分辨率 resolution	3.200 Å
最低分辨率 resolution_low	208.510 Å
R 因子 r_factor	0.2140
R_work r_work	0.2120
R_free r_free	0.2570

fit	/app/data/saxs_computation/j0/2j04/2j04.fit
pddf	/app/data/pr_computation/j0/2j04/2j04.pr.out
profile	/app/data/saxs_computation/j0/2j04/2j04.dat

The tau60-tau91 subcomplex of yeast transcription factor IIIC

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

衍射实验 Diffraction

5. 实体与聚合体信息 (3)

Entity 1 polymer

聚合体 Polymer

来源 Source

Entity 2 polymer

聚合体 Polymer

来源 Source

Entity 3 water

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)