SAXSdb — 9gc3

▼

1. 结构基本信息 1. Structure Basics

条目编号 entry_id	9gc3
沉积日期 deposition_date	2024-08-01
结构标题 title	yeast TFIIIC TauB subcomplex bound to a tRNA gene
关键词 keywords	Base-specific contacts, B-box, tRNA gene, DNA BINDING PROTEIN; DNA BINDING PROTEIN
实验方法 method	ELECTRON MICROSCOPY

▼

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier	42.60 Å
回转半径 Rg (电子) rg_electron	41.92 Å
零角强度 I(0) i0	758360000.00
分子量 molecular_weight	215010.0 kDa
排除体积 excluded_volume	264210 Å³
包络体积 envelope_volume	360850 Å³
水化壳体积 shell_volume	70954 Å³
包络直径 envelope_diameter	148.1 Å
壳层 Rg shell_rg	47.62 Å
包络 Rg envelope_rg	41.88 Å
形状 Rg shape_rg	41.88 Å
总 Rg total_rg	42.30 Å
总原子数 total_atoms	15046
残基数 n_residues	1753
球谐函数阶数 n_harmonics	20
q 范围 q_range	— – 0.5000 Å⁻¹
数据点数 n_points	101
壳层类型 shell_type	directional
溶剂电子密度 solvent_density	0.3340 e/Å³
壳层衬度 contrast_shell	0.0300 e/Å³
CRYSOL 版本 crysol_version	4.1.3

▼

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax	141.5 Å
Rg (实空间) rg_real	42.54 Å
Rg 误差 (实空间) rg_real_error	1.71 Å
I(0) (实空间) i0_real	7.5840e+08
I(0) 误差 (实空间) i0_real_error	1.5300e+07
Rg (倒空间) rg_reciprocal	42.60 Å
I(0) (倒空间) i0_reciprocal	758400000.0000
解质量估计 total_estimate	0.8924
解质量评级 solution_quality	GOOD a GOOD solution
P(r) 峰数 n_peaks	2
主峰位置 r_peak_primary	49.7 Å
偏度 Skewness skewness	0.277
峰度 Kurtosis kurtosis	-0.456
角度范围 angular_range	— – 0.1850 Å⁻¹
当前正则化参数 α current_alpha	0.0000
最高正则化参数 α highest_alpha	78150000.0000
实空间数据点数 n_real_points	38
GNOM 版本 gnom_version	4.1.3
质量判据 quality_criteria	AN1: 0.000; Oscil: 0.890; Stabil: 0.999; Sysdev: 1.000; Positv: 1.000; Valcen: 1.000; Smooth: 0.929

▶

4. 晶体学与实验 4. Crystallography & Experiment

▶

5. 实体与聚合体信息 (5)

Entity 1 polymer

描述 description	Transcription factor tau 138 kDa subunit
分子量 formula_weight	136602.5 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	A
原子数 atom_count	15046

MVLTIYPDELVQIVSDKIASNKGKITLNQLWDISGKYFDLSDKKVKQFVLSCVILKKDIEVYCDGAITTKNVTDIIGDANHSYSVGITEDSLWTLLTGYTKKESTIGNSAFELLLEVAKSGEKGINTMDLAQVTGQDPRSVTGRIKKINHLLTSSQLIYKGHVVKQLKLKKFSHDGVDSNPYINIRDHLATIVEVVKRSKNGIRQIIDLKRELKFDKEKRLSKAFIAAIAWLDEKEYLKKVLVVSPKNPAIKIRCVKYVKDIPDSKGSPSFEYDSNSADEDSVSDSKAAFEDEDLVEGLDNFNATDLLQNQGLVMEEKEDAVKNEVLLNRFYPLQNQTYDIADKSGLKGISTMDVVNRITGKEFQRAFTKSSEYYLESVDKQKENTGGYRLFRIYDFEGKKKFFRLFTAQNFQKLTNAEDEISVPKGFDELGKSRTDLKTLNEDNFVALNNTVRFTTDSDGQDIFFWHGELKIPPNSKKTPNKNKRKRQVKNSTNASVAGNISNPKRIKLEQHVSTAQEPKSAEDSPSSNGGTVVKGKVVNFGGFSARSLRSLQRQRAILKVMNTIGGVAYLREQFYESVSKYMGSTTTLDKKTVRGDVDLMVESEKLGARTEPVSGRKIIFLPTVGEDAIQRYILKEKDSKKATFTDVIHDTEIYFFDQTEKNRFHRGKKSVERIRKFQNRQKNAKIKASDDAISKKSTSVNVSDGKIKRRDKKVSAGRTTVVVENTKEDKTVYHAGTKDGVQALIRAVVVTKSIKNEIMWDKITKLFPNNSLDNLKKKWTARRVRMGHSGWRAYVDKWKKMLVLAIKSEKISLRDVEELDLIKLLDIWTSFDEKEIKRPLFLYKNYEENRKKFTLVRDDTLTHSGNDLAMSSMIQREISSLKKTYTRKISASTKDLSKSQSDDYIRTVIRSILIESPSTTRNEIEALKNVGNESIDNVIMDMAKEKQIYLHGSKLECTDTLPDILENRGNYKDFGVAFQYRCKVNELLEAGNAIVINQEPSDISSWVLIDLISGELLNMDVIPMVRNVRPLTYTSRRFEIRTLTPPLIIYANSQTKLNTARKSAVKVPLGKPFSRLWVNGSGSIRPNIWKQVVTMVVNEIIFHPGITLSRLQSRCREVLSLHEISEICKWLLERQVLITTDFDGYWVNHNWYSIYESTENLYFQSMSASAWSHPQFEKGGGSGGGSGGSAWSHPQFEKS

来源 Source

来源生物 organism	Saccharomyces cerevisiae
通用名 common_name	;brewer's yeast ;
NCBI 分类编号 ncbi_taxonomy_id	4932
宿主生物 host_organism	Spodoptera frugiperda

Entity 2 polymer

描述 description	Transcription factor tau 91 kDa subunit
分子量 formula_weight	76484.1 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	B

HHHHHHENLYFQGMAVIPAKKRGRPRKSVVAEVPYDSLASPVSENSGSKRPRRNASKKAVANFAQLVHAGRDDVINTTQVNNVDDTDDDDFVLNDEGDGEESDNVEIEFENELESTKNEVADLNSSGSGASVRPSGRRNTVQKLRLKKNSTKNMKSSSPGSSLGQKGRPIRLLKDLSSARDKIERIYGLNKEKLLLLAKVKEGFETSVFDFPFKNIQPDSPYFVCLDPPCKKESAYNKVIGDKNRTVYHEINKTEFENMIKLRTKRLKLLIGEVDAEVSTGDKIEFPVLANGKRRGFIYNVGGLVTDIAWLNIEENTDIGKDIQYLAVAVSQYMDEPLNEHLEMFDKEKHSSCIQIFKMNTSTLHCVKVQTIVHSFGEVWDLKWHEGCHAPHLVGCLSFVSQEGTINFLEIIDNATDVHVFKMCEKPSLTLSLADSLITTFDFLSPTTVVCGFKNGFVAEFDLTDPEVPSFYDQVHDSYILSVSTAYSDFEDTVVSTVAVDGYFYIFNPKDIATTKTTVSRFRGSNLVPVVYCPQIYSYIYSDGASSLRAVPSRAAFAVHPLVSRETTITAIGVSRLHPMVLAGSADGSLIITNAARRLLHGIKNSSATQKSLRLWKWDYSIKDDKYRIDSSYEVYPLTVNDVSKAKIDAHGINITCTKWNETSAGGKCYAFSNSAGLLTLEYLS

来源 Source

来源生物 organism	Saccharomyces cerevisiae
通用名 common_name	;brewer's yeast ;
NCBI 分类编号 ncbi_taxonomy_id	4932
宿主生物 host_organism	Spodoptera frugiperda

Entity 3 polymer

描述 description	Transcription factor tau 60 kDa subunit
分子量 formula_weight	67755.2 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polypeptide(L)
链编号 strand_id	C

MKLLKDLLVDRKEFEDWKNNLTWARDGTLYLTTFPDISIGQPKYAKDINCNSKNLFHVKEFPLEFENKLDFELAQQNGLLNSQPVCYPRVCKPSPIDDWMAVLSNNGNVSVFKDNKMLTNLDSKGNLSSRTYHCFEWNPIESSIVVGNEDGELQFFSIRKNSENTPEFYFESSIRLSDAGSKDWVTHIVWYEDVLVAALSNNSVFSMTVSASSHQPVSRMIQNASRRKITDLKIVDYKVVLTCPGYVHKIDLKNYSISSLKTGSLENFHIIPLNHEKESTILLMSNKTSYKVLLEDELHVTADNIIAPYLEKKFKKWSTIWNEFNNYETTLVIHGISLSPDGYSIAIVYDMERVAFKYKIASEQSFNIMFAPLYHTWTISERAVGLAWYQTYQIYNQSLPKLPENFSMNKKLLNGNYPISLDFQSYLNALMKSEEMRIIMFLNMTIDKPSILSFLEALYEYAINKKSELTNSFDLACVLSIAAILKREAPIYNGTLLMKNSFLEETFNLESFTADPETVTSTTNNTWKRCGVTLLPILTTHVKICPVSKQRVIDIKRDDLNDYGWFTRGLLERFNEISVYCGTTLEVM

来源 Source

来源生物 organism	Saccharomyces cerevisiae
通用名 common_name	;brewer's yeast ;
NCBI 分类编号 ncbi_taxonomy_id	4932
宿主生物 host_organism	Spodoptera frugiperda

Entity 4 polymer

描述 description	DNA (40-MER)
分子量 formula_weight	12358.9 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polydeoxyribonucleotide
链编号 strand_id	D

GCCGATGAAACCCTGGTTCGATTCTAGGAGATGGCATTTT

来源 Source

来源生物 organism	Saccharomyces cerevisiae
NCBI 分类编号 ncbi_taxonomy_id	4932
宿主生物 host_organism	Spodoptera frugiperda

Entity 5 polymer

描述 description	DNA (40-MER)
分子量 formula_weight	12265.9 kDa
来源方法 src_method	man
拷贝数 entity_count	1

聚合体 Polymer

聚合体类型 type	polydeoxyribonucleotide
链编号 strand_id	E

AAAATGCCATCTCCTAGAATCGAACCAGGGTTTCATCGGC

来源 Source

来源生物 organism	Saccharomyces cerevisiae
NCBI 分类编号 ncbi_taxonomy_id	4932
宿主生物 host_organism	Spodoptera frugiperda

▶

7. 引用文献 (1)

▶

晶胞边长 a length_a	1.000 Å
晶胞边长 b length_b	1.000 Å
晶胞边长 c length_c	1.000 Å
晶胞夹角 α angle_alpha	90.00 °
晶胞夹角 β angle_beta	90.00 °
晶胞夹角 γ angle_gamma	90.00 °
晶胞分子数 Z z_pdb	—

空间群 space_group	P 1
空间群编号 int_tables_number	1
晶系 crystal_system	—

分辨率 resolution	2.460 Å
最低分辨率 resolution_low	2.460 Å
R 因子 r_factor	0.3223
R_work r_work	0.3223
R_free r_free	—

分辨率 resolution	2.46 Å
分辨率测定方法 resolution_method	—
颗粒数 num_particles	570437

fit	/app/data/saxs_computation/gc/9gc3/9gc3.fit
pddf	/app/data/pr_computation/gc/9gc3/9gc3.pr.out
profile	/app/data/saxs_computation/gc/9gc3/9gc3.dat

yeast TFIIIC TauB subcomplex bound to a tRNA gene

SAXS 散射曲线 SAXS Profile

P(r) 距离分布 P(r) Distribution

1. 结构基本信息 1. Structure Basics

2. SAXS 参数 (CRYSOL 理论计算) 2. SAXS Parameters (CRYSOL)

3. P(r) 距离分布 (GNOM 反演) 3. P(r) Analysis (GNOM)

4. 晶体学与实验 4. Crystallography & Experiment

晶胞参数 Unit Cell

对称性 Symmetry

精修参数 Refinement

冷冻电镜 EM 3D

5. 实体与聚合体信息 (5)

Entity 1 polymer

聚合体 Polymer

来源 Source

Entity 2 polymer

聚合体 Polymer

来源 Source

Entity 3 polymer

聚合体 Polymer

来源 Source

Entity 4 polymer

聚合体 Polymer

来源 Source

Entity 5 polymer

聚合体 Polymer

来源 Source

7. 引用文献 (1)

8. 文件与曲线 (10)

Files (7)

Curves (3)