8ffz

TFIIIA-TFIIIC-Brf1-TBP complex bound to 5S rRNA gene

Method: ELECTRON MICROSCOPY
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Transcription factor IIIA

Saccharomyces cerevisiae

UniProt P39933

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Heteromer Protein 8 DNA 2 Transcription factor tau 138 kDa subunit × 1 (P34111) Transcription factor tau 131 kDa subunit × 1 (P33339) Transcription factor tau 95 kDa subunit × 1 (P32367) Transcription factor tau 91 kDa subunit × 1 (Q06339) Transcription factor tau 60 kDa subunit × 1 (Q12308) Transcription factor tau 55 kDa subunit × 1 (Q12415) Transcription factor IIIB 70 kDa subunit,TATA-box-binding protein × 1 (P13393,P29056) DNA (151-MER) × 1 DNA (151-MER) × 1 ZINC ION × 9 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name TF3A_YEAST
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A; PDBConstruct 1–429; UniProt 1–429

Transcription factor tau 138 kDa subunit

Saccharomyces cerevisiae

UniProt P34111

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Heteromer Protein 8 DNA 2 Transcription factor IIIA × 1 (P39933) Transcription factor tau 131 kDa subunit × 1 (P33339) Transcription factor tau 95 kDa subunit × 1 (P32367) Transcription factor tau 91 kDa subunit × 1 (Q06339) Transcription factor tau 60 kDa subunit × 1 (Q12308) Transcription factor tau 55 kDa subunit × 1 (Q12415) Transcription factor IIIB 70 kDa subunit,TATA-box-binding protein × 1 (P13393,P29056) DNA (151-MER) × 1 DNA (151-MER) × 1 ZINC ION × 9 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name TFC3_YEAST
Isoform —
PDB entities 2
Chains and sequence ranges Author chain B; PDBConstruct 1–1160; UniProt 1–1160

Transcription factor tau 131 kDa subunit

Saccharomyces cerevisiae

UniProt P33339

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Heteromer Protein 8 DNA 2 Transcription factor IIIA × 1 (P39933) Transcription factor tau 138 kDa subunit × 1 (P34111) Transcription factor tau 95 kDa subunit × 1 (P32367) Transcription factor tau 91 kDa subunit × 1 (Q06339) Transcription factor tau 60 kDa subunit × 1 (Q12308) Transcription factor tau 55 kDa subunit × 1 (Q12415) Transcription factor IIIB 70 kDa subunit,TATA-box-binding protein × 1 (P13393,P29056) DNA (151-MER) × 1 DNA (151-MER) × 1 ZINC ION × 9 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name TFC4_YEAST
Isoform —
PDB entities 3
Chains and sequence ranges Author chain C; PDBConstruct 1–1025; UniProt 1–1025

Transcription factor tau 95 kDa subunit

Saccharomyces cerevisiae

UniProt P32367

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Heteromer Protein 8 DNA 2 Transcription factor IIIA × 1 (P39933) Transcription factor tau 138 kDa subunit × 1 (P34111) Transcription factor tau 131 kDa subunit × 1 (P33339) Transcription factor tau 91 kDa subunit × 1 (Q06339) Transcription factor tau 60 kDa subunit × 1 (Q12308) Transcription factor tau 55 kDa subunit × 1 (Q12415) Transcription factor IIIB 70 kDa subunit,TATA-box-binding protein × 1 (P13393,P29056) DNA (151-MER) × 1 DNA (151-MER) × 1 ZINC ION × 9 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name TFC1_YEAST
Isoform —
PDB entities 4
Chains and sequence ranges Author chain D; PDBConstruct 1–649; UniProt 1–649

Transcription factor tau 91 kDa subunit

Saccharomyces cerevisiae

UniProt Q06339

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Heteromer Protein 8 DNA 2 Transcription factor IIIA × 1 (P39933) Transcription factor tau 138 kDa subunit × 1 (P34111) Transcription factor tau 131 kDa subunit × 1 (P33339) Transcription factor tau 95 kDa subunit × 1 (P32367) Transcription factor tau 60 kDa subunit × 1 (Q12308) Transcription factor tau 55 kDa subunit × 1 (Q12415) Transcription factor IIIB 70 kDa subunit,TATA-box-binding protein × 1 (P13393,P29056) DNA (151-MER) × 1 DNA (151-MER) × 1 ZINC ION × 9 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name TFC6_YEAST
Isoform —
PDB entities 5
Chains and sequence ranges Author chain E; PDBConstruct 1–672; UniProt 1–672

Transcription factor tau 60 kDa subunit

Saccharomyces cerevisiae

UniProt Q12308

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Heteromer Protein 8 DNA 2 Transcription factor IIIA × 1 (P39933) Transcription factor tau 138 kDa subunit × 1 (P34111) Transcription factor tau 131 kDa subunit × 1 (P33339) Transcription factor tau 95 kDa subunit × 1 (P32367) Transcription factor tau 91 kDa subunit × 1 (Q06339) Transcription factor tau 55 kDa subunit × 1 (Q12415) Transcription factor IIIB 70 kDa subunit,TATA-box-binding protein × 1 (P13393,P29056) DNA (151-MER) × 1 DNA (151-MER) × 1 ZINC ION × 9 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name TFC8_YEAST
Isoform —
PDB entities 6
Chains and sequence ranges Author chain F; PDBConstruct 1–588; UniProt 1–588

Transcription factor tau 55 kDa subunit

Saccharomyces cerevisiae

UniProt Q12415

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Heteromer Protein 8 DNA 2 Transcription factor IIIA × 1 (P39933) Transcription factor tau 138 kDa subunit × 1 (P34111) Transcription factor tau 131 kDa subunit × 1 (P33339) Transcription factor tau 95 kDa subunit × 1 (P32367) Transcription factor tau 91 kDa subunit × 1 (Q06339) Transcription factor tau 60 kDa subunit × 1 (Q12308) Transcription factor IIIB 70 kDa subunit,TATA-box-binding protein × 1 (P13393,P29056) DNA (151-MER) × 1 DNA (151-MER) × 1 ZINC ION × 9 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name TFC7_YEAST
Isoform —
PDB entities 7
Chains and sequence ranges Author chain G; PDBConstruct 1–435; UniProt 1–435

Transcription factor IIIB 70 kDa subunit,TATA-box-binding protein

Saccharomyces cerevisiae S288C

UniProt P13393

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Heteromer Protein 8 DNA 2 Transcription factor IIIA × 1 (P39933) Transcription factor tau 138 kDa subunit × 1 (P34111) Transcription factor tau 131 kDa subunit × 1 (P33339) Transcription factor tau 95 kDa subunit × 1 (P32367) Transcription factor tau 91 kDa subunit × 1 (Q06339) Transcription factor tau 60 kDa subunit × 1 (Q12308) Transcription factor tau 55 kDa subunit × 1 (Q12415) DNA (151-MER) × 1 DNA (151-MER) × 1 ZINC ION × 9 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name TBP_YEAST
Isoform —
PDB entities 8
Chains and sequence ranges Author chain H; PDBConstruct 387–566; UniProt 61–240

Transcription factor IIIB 70 kDa subunit,TATA-box-binding protein

Saccharomyces cerevisiae S288C

UniProt P29056

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Heteromer Protein 8 DNA 2 Transcription factor IIIA × 1 (P39933) Transcription factor tau 138 kDa subunit × 1 (P34111) Transcription factor tau 131 kDa subunit × 1 (P33339) Transcription factor tau 95 kDa subunit × 1 (P32367) Transcription factor tau 91 kDa subunit × 1 (Q06339) Transcription factor tau 60 kDa subunit × 1 (Q12308) Transcription factor tau 55 kDa subunit × 1 (Q12415) DNA (151-MER) × 1 DNA (151-MER) × 1 ZINC ION × 9 Consistent with all polymers

Other States of the Same Protein in the Database

View Construct and Data Evidence
UniProt name TF3B_YEAST
Isoform —
PDB entities 8
Chains and sequence ranges Author chain H; PDBConstruct 1–382; UniProt 1–382 Author chain H; PDBConstruct 579–736; UniProt 439–596

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id8ffz
Deposition date deposition_date2022-12-11
Structure title titleTFIIIA-TFIIIC-Brf1-TBP complex bound to 5S rRNA gene
Keywords keywordstranscription factor, TRANSCRIPTION, TRANSCRIPTION-DNA complex; TRANSCRIPTION/DNA
Experimental Method methodELECTRON MICROSCOPY
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

8ffz__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

8ffz__assembly_1__model_1 | I(q)

10-2 10-1 106 107 108 109 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

8ffz__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg(Guinier)75.19 Å
Rg (electron density)74.46 Å
Total Rg74.63 Å
Atom count38962
Residues4347
Excluded volume677080 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 8ffz__assembly_1__model_1 decameric (10) Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (11)

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6. Fold Classification (SCOP + CATH) 1 domains

CATH v4.4 (1 domains)

Domain ID domain_id8ffzG01
Class class3 — Alpha Beta
Architecture architecture40 — 3-Layer(aba) Sandwich
Topology topology50 — Rossmann fold
Homologous superfamily homologous superfamily1240 — Phosphoglycerate mutase-like
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7. Citations (2)