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2kbt

Attachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method

Method: SOLUTION NMR
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1. Protein Identity and Related Structures Protein Identity & Related Structures

Proto-oncogene vav,Immunoglobulin G-binding protein G

;Streptococcus sp. 'group G' ;

UniProt P06654

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Monomer Protein 1 No other associated polymer Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1em7 1gb1 1igd 1le3 1pga 1pgb 1pgx 2gb1 2igd 2igh 2j52 2j53

30 assemblies; showing the first 12

Homodimer
1q10 2rmm 3mp9

3 assemblies

Homotetramer
1mpe 1mvk 1mvk 1mvk

4 assemblies

Other homooligomers

None

Heteromeric or nucleic-acid complex
1igc 1pn5 2cwb 2den 2kbt 2mbb 6v9i 7rxc 7rxd 8jxr 8jxs 9bdt

18 assemblies; showing the first 12

View Construct and Data Evidence
UniProt name SPG1_STRSG
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 78–132； UniProt 228–282

Proto-oncogene vav,Immunoglobulin G-binding protein G

;Streptococcus sp. 'group G' ;

UniProt P27870

State in the Current Structure

Assembly Physical composition Protein state Molecular copy count Associated components Data consistency
1 Insufficient information Monomer Protein 1 No other associated polymer Consistent with protein count

Other States of the Same Protein in the Database

Monomer
1f5x 1gcp 1gcp 1gcp 1gcp 1k1z

6 assemblies

Homodimer

None

Homotetramer

None

Other homooligomers

None

Heteromeric or nucleic-acid complex
1gcq 2kbt 2vrw

3 assemblies

View Construct and Data Evidence
UniProt name VAV_MOUSE
Isoform —
PDB entities 1
Chains and sequence ranges Author chain A； PDBConstruct 5–65； UniProt 784–844

The page prioritizes protein identity, the current assembly, associated components, oligomeric state and cross-PDB links. Chain mapping and sequence ranges are retained as data evidence. Internal IDs, import timestamps and assembly operation expressions are maintenance fields and are not shown here.

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2. Structure Basics 2. Structure Basics

Entry ID entry_id2kbt
Deposition date deposition_date2008-12-07
Structure title titleAttachment of an NMR-invisible solubility enhancement tag (INSET) using a sortase-mediated protein ligation method
Keywords keywordssortase, protein ligation, GB1, intein, INSET, solubility enhancement, SIGNALING PROTEIN; SIGNALING PROTEIN
Experimental Method methodSOLUTION NMR
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3. Official assembly/model SAXS Official SAXS Profiles

This page reads only the latest assembly calculations. Every curve maps to an explicit PDB entry, official biological assembly and coordinate model.

2kbt__assembly_1__model_1

Assembly 1 · Model 1 · CRYSOL 4.1.3-1-20251215 (887e7ef)

Download this curve (.dat)

2kbt__assembly_1__model_1 | I(q)

10-2 10-1 105 106 q (1/Angstrom) I(q)
100 plotted points; both axes use logarithmic scales.

2kbt__assembly_1__model_1 | P(r) · Pending

The new assembly/model P(r) has not been calculated yet This placeholder does not display legacy data r (Angstrom) P(r)
P(r) will be calculated and displayed separately for the same assembly/model.
Rg（Guinier）13.67 Å
Rg (electron density)12.19 Å
Total Rg13.62 Å
Atom count1102
Residues69
Excluded volume9952 ų
Maximum q0.500 Å⁻¹
Assembly Model Structure unit Oligomeric description Status CRYSOL Actions
1 1 2kbt__assembly_1__model_1 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 2 2kbt__assembly_1__model_2 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 3 2kbt__assembly_1__model_3 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 4 2kbt__assembly_1__model_4 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 5 2kbt__assembly_1__model_5 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 6 2kbt__assembly_1__model_6 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 7 2kbt__assembly_1__model_7 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 8 2kbt__assembly_1__model_8 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 9 2kbt__assembly_1__model_9 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 10 2kbt__assembly_1__model_10 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 11 2kbt__assembly_1__model_11 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 12 2kbt__assembly_1__model_12 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 13 2kbt__assembly_1__model_13 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 14 2kbt__assembly_1__model_14 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 15 2kbt__assembly_1__model_15 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 16 2kbt__assembly_1__model_16 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 17 2kbt__assembly_1__model_17 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 18 2kbt__assembly_1__model_18 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 19 2kbt__assembly_1__model_19 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
1 20 2kbt__assembly_1__model_20 monomeric （1） Success 4.1.3-1-20251215 (887e7ef) View Download
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4. Crystallography and Experiment 4. Crystallography & Experiment

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5. Entities and Polymers Entities & Polymers (1)

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6. Fold Classification (SCOP + CATH) 1 domains

CATH v4.4 (1 domains)

Domain ID domain_id2kbtA00
Class class2 — Mainly Beta
Architecture architecture30 — Roll
Topology topology30 — SH3 type barrels.
Homologous superfamily homologous superfamily40 — SH3 Domains
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7. Citations (1)