3eff

The Crystal Structure of Full-Length KcsA in its Closed Conformation

Method: X-RAY DIFFRACTION Dmax: 159.0 Å Quality: GOOD

1. 蛋白身份与相关结构 Protein Identity & Related Structures

Voltage-gated potassium channel

物种未注明

UniProt P0A334

当前结构中的状态

Assembly 聚集状态 构建体 突变与修饰 配体、离子与共同组分 实验方法与环境 结构质量
1 蛋白异源复合物 异源复合物 蛋白 × 8 PDB 声明:octameric(8) 与蛋白拷贝数一致 链 K; UniProt 22–160 链 L; UniProt 22–160 链 M; UniProt 22–160 链 N; UniProt 22–160 片段:UNP residues 22-160 FAB × 2 FAB × 2 X-RAY DIFFRACTION X-ray结晶条件:VAPOR DIFFUSION, SITTING DROP;pH 5.6;298 K;340 mM (NH4)2SO4, 11% PEG4000, 100 mM Na3C6H5O7 pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K 分辨率 3.80 Å R-free 0.331

数据库中的同蛋白其他状态

以下每一行都是同一 UniProt 蛋白在另一个 PDB 条目中的 biological assembly, “相对当前条目”直接指出证据层面的不同;没有差异标签表示当前已读取字段一致。

共 86 个其他 PDB 条目、96 个 assembly。 打开独立比较页并筛选聚集状态

查看构建体与数据证据
UniProt名称 KCSA_STRLI
Isoform
PDB实体 3
链与序列区间 作者链 K; PDB构建体 1–139; UniProt 22–160 作者链 L; PDB构建体 1–139; UniProt 22–160 作者链 M; PDB构建体 1–139; UniProt 22–160 作者链 N; PDB构建体 1–139; UniProt 22–160

页面优先展示蛋白身份、当前 assembly、共同组分、聚集状态和跨 PDB 结构链接。 链映射与序列区间收在“数据证据”中;数据库内部编号、导入时间和 assembly 操作表达式仅用于维护,因此不在读者页面展示。

SAXS 散射曲线 SAXS Profile

SAXS profile for 3eff

P(r) 距离分布 P(r) Distribution

P(r) distribution for 3eff
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2. 结构基本信息 2. Structure Basics

条目编号 entry_id3eff
沉积日期 deposition_date2008-09-08
结构标题 titleThe Crystal Structure of Full-Length KcsA in its Closed Conformation
关键词 keywords;Full length KcsA, Bulge helix, Cell membrane, Ion transport, Ionic channel, Membrane, Transmembrane, Transport, Voltage-gated channel, MEMBRANE PROTEIN ;; MEMBRANE PROTEIN
实验方法 methodX-RAY DIFFRACTION

3. SAXS 参数 (CRYSOL 理论计算) 3. SAXS Parameters (CRYSOL)

回转半径 Rg (Guinier) rg_guinier53.54
回转半径 Rg (电子) rg_electron54.67
零角强度 I(0) i0344900000.00
分子量 molecular_weight154520.0 kDa
排除体积 excluded_volume193650 ų
包络体积 envelope_volume287150 ų
水化壳体积 shell_volume48201 ų
包络直径 envelope_diameter170.1
壳层 Rg shell_rg48.51
包络 Rg envelope_rg53.73
形状 Rg shape_rg54.62
总 Rg total_rg54.60
总原子数 total_atoms10912
残基数 n_residues1424
球谐函数阶数 n_harmonics20
q 范围 q_range— – 0.5000 −1
数据点数 n_points101
壳层类型 shell_typedirectional
溶剂电子密度 solvent_density0.3340 e/ų
壳层衬度 contrast_shell0.0300 e/ų
CRYSOL 版本 crysol_version4.1.3

4. P(r) 距离分布 (GNOM 反演) 4. P(r) Analysis (GNOM)

最大尺寸 Dmax dmax159.0
Rg (实空间) rg_real53.68
Rg 误差 (实空间) rg_real_error1.43
I(0) (实空间) i0_real3.4490e+08
I(0) 误差 (实空间) i0_real_error6.2870e+06
Rg (倒空间) rg_reciprocal53.38
I(0) (倒空间) i0_reciprocal344700000.0000
解质量估计 total_estimate0.8003
解质量评级 solution_quality GOOD a GOOD solution
P(r) 峰数 n_peaks3
主峰位置 r_peak_primary32.9
偏度 Skewness skewness0.153
峰度 Kurtosis kurtosis-0.970
角度范围 angular_range— – 0.1450 −1
当前正则化参数 α current_alpha0.0000
最高正则化参数 α highest_alpha12830000.0000
实空间数据点数 n_real_points30
GNOM 版本 gnom_version4.1.3
质量判据 quality_criteria AN1: 0.000; Oscil: 0.871; Stabil: 1.000; Sysdev: 1.000; Positv: 1.000; Valcen: 0.787; Smooth: 0.000

5. 晶体学与实验 5. Crystallography & Experiment

6. 实体与聚合物信息 Entities & Polymers (3)

7. 折叠分类 (SCOP + CATH) 16 domains

CATH v4.4 (16 domains)

结构域编号 domain_id3effA01
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id3effA02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id3effB01
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id3effB02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id3effC01
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id3effC02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id3effD01
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id3effD02
类 Class class2 — Mainly Beta
架构 Architecture architecture60 — Sandwich
拓扑 Topology topology40 — Immunoglobulin-like
同源超家族 H-superfamily homologous superfamily10 — Immunoglobulins
结构域编号 domain_id3effK01
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily70
结构域编号 domain_id3effK02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily440 — ATP synthase delta/epsilon subunit, C-terminal domain
结构域编号 domain_id3effL01
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily70
结构域编号 domain_id3effL02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily440 — ATP synthase delta/epsilon subunit, C-terminal domain
结构域编号 domain_id3effM01
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily70
结构域编号 domain_id3effM02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily440 — ATP synthase delta/epsilon subunit, C-terminal domain
结构域编号 domain_id3effN01
类 Class class1 — Mainly Alpha
架构 Architecture architecture10 — Orthogonal Bundle
拓扑 Topology topology287 — Helix Hairpins
同源超家族 H-superfamily homologous superfamily70
结构域编号 domain_id3effN02
类 Class class1 — Mainly Alpha
架构 Architecture architecture20 — Up-down Bundle
拓扑 Topology topology5 — Single alpha-helices involved in coiled-coils or other helix-helix interfaces
同源超家族 H-superfamily homologous superfamily440 — ATP synthase delta/epsilon subunit, C-terminal domain

8. 引用文献 (1)

9. 文件与曲线 (10)